##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4373 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.94168762863023 23.0 12.0 32.0 12.0 34.0 2 21.1651040475646 21.0 11.0 32.0 11.0 34.0 3 21.71324033844043 23.0 11.0 32.0 11.0 34.0 4 20.831694488909214 21.0 11.0 32.0 11.0 34.0 5 20.51703635947862 21.0 11.0 31.0 11.0 34.0 6 21.187514292247887 21.0 11.0 33.0 11.0 37.0 7 21.169448890921565 21.0 11.0 32.0 11.0 37.0 8 21.361765378458724 21.0 11.0 31.0 11.0 37.0 9 22.542876743654244 21.0 12.0 34.0 12.0 37.0 10-11 21.658358106563 21.0 11.0 33.5 11.0 37.0 12-13 21.739080722616052 21.0 11.0 34.0 11.0 37.0 14-15 21.43436999771324 21.0 11.0 33.0 11.0 37.0 16-17 20.844157329064714 21.0 11.0 31.0 11.0 37.0 18-19 21.291561856848844 21.0 11.0 32.0 11.0 37.0 20-21 21.486622455979877 21.0 11.0 32.0 11.0 37.0 22-23 20.907614909672994 21.0 11.0 31.5 11.0 37.0 24-25 21.009604390578552 21.0 11.0 31.0 11.0 37.0 26-27 20.82003201463526 21.0 11.0 31.0 11.0 37.0 28-29 20.22513148868054 16.0 11.0 31.0 11.0 36.0 30-31 20.110679167619484 11.0 11.0 29.5 11.0 37.0 32-33 20.52138120283558 21.0 11.0 29.5 11.0 36.5 34-35 20.977589755316714 21.0 11.0 31.0 11.0 36.0 36-37 20.586668191173107 21.0 11.0 29.0 11.0 36.0 38-39 19.857077521152526 11.0 11.0 27.0 11.0 36.0 40-41 20.122798993825747 16.0 11.0 27.0 11.0 36.0 42-43 20.57237594328836 21.0 11.0 29.0 11.0 36.5 44-45 19.992225017150698 15.5 11.0 27.0 11.0 36.0 46-47 20.302881317173565 16.0 11.0 27.0 11.0 36.0 48-49 20.5882689229362 21.0 11.0 29.0 11.0 36.0 50-51 20.825634575806085 21.0 11.0 31.0 11.0 36.0 52-53 20.816030185227532 21.0 11.0 31.0 11.0 36.0 54-55 20.796592728104276 21.0 11.0 31.0 11.0 36.0 56-57 20.076606448662247 16.0 11.0 27.0 11.0 36.0 58-59 20.23747999085296 16.0 11.0 29.0 11.0 36.0 60-61 20.466384632975075 21.0 11.0 29.0 11.0 36.0 62-63 19.84895952435399 19.0 11.0 27.0 11.0 36.0 64-65 20.245483649668422 20.0 11.0 28.5 11.0 36.0 66-67 20.619483192316487 20.0 11.0 30.0 11.0 36.0 68-69 20.185913560484792 19.0 11.0 28.5 11.0 36.0 70-71 19.771552709810198 19.0 11.0 27.0 11.0 35.0 72-73 20.223645094900526 19.0 11.0 28.0 11.0 35.0 74-75 19.916190258403844 19.5 11.0 27.5 11.0 34.5 76 19.18088268922936 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -3.7982547488902476 1101 2 -2.625671419201442 1101 3 0.321077138149807 1101 4 -0.6344956157985742 1101 5 -3.289623723030722 1101 6 1.0687680977704659 1101 7 -2.7689170373513328 1101 8 2.1473819440476625 1101 9 -0.22348273671090624 1101 10-11 -2.2976050117436344 1101 12-13 0.988885791032601 1101 14-15 -3.378696160639194 1101 16-17 -0.4224150101365183 1101 18-19 -5.064754453888497 1101 20-21 -2.5483984501842407 1101 22-23 -4.434667158855486 1101 24-25 -3.752228301397782 1101 26-27 -4.500573997646569 1101 28-29 -3.1040000628388675 1101 30-31 -2.1818162170013657 1101 32-33 -1.7376147713135452 1101 34-35 -0.39983781370350613 1101 36-37 -5.413092923520452 1101 38-39 -3.8615315885230928 1101 40-41 -4.617292355013859 1101 42-43 -4.052967718397696 1101 44-45 -1.5013684170260149 1101 46-47 -2.334534179553632 1101 48-49 -2.7103628663964514 1101 50-51 -5.344414515890303 1101 52-53 -2.9925041848981664 1101 54-55 -1.3207864290264055 1101 56-57 -3.903166485262787 1101 58-59 -3.275256286620248 1101 60-61 -2.3680849411031133 1101 62-63 -3.3965357421193865 1101 64-65 -3.397024054207435 1101 66-67 -0.3849918055235193 1101 68-69 -1.3109812833048977 1101 70-71 -2.9744746451631983 1101 72-73 -4.999397698410348 1101 74-75 -3.736476032115185 1101 76 -3.6158881098397586 1102 1 -0.7714690346045323 1102 2 -2.0899571334871574 1102 3 -2.928922861850193 1102 4 0.6958615270585682 1102 5 0.0675191341121355 1102 6 -0.7615890450866765 1102 7 -4.38498846592276 1102 8 -2.6383323416666222 1102 9 -1.259197022425191 1102 10-11 1.305073559684935 1102 12-13 -4.600399923253114 1102 14-15 -0.18226758921062114 1102 16-17 -1.060807867279376 1102 18-19 -2.7701115967456396 1102 20-21 -5.334112735898525 1102 22-23 -1.0060957302840556 1102 24-25 -0.3950854442549243 1102 26-27 0.017283145210576834 1102 28-29 -2.550428634267437 1102 30-31 -4.101459074144223 1102 32-33 -0.7465433427421182 1102 34-35 -4.524837813703506 1102 36-37 -2.5291643520918825 1102 38-39 -0.3481387313802351 1102 40-41 -3.0815780692995745 1102 42-43 0.03631799588801954 1102 44-45 -3.37190413131173 1102 46-47 -2.9238198938393474 1102 48-49 -3.045184294967882 1102 50-51 -3.1033430873188728 1102 52-53 -4.229111327755312 1102 54-55 -2.7270364290264055 1102 56-57 -1.8763807709770717 1102 58-59 -3.935970572334533 1102 60-61 -1.046656369674544 1102 62-63 0.5007856864520406 1102 64-65 -0.6380954827788621 1102 66-67 -2.84481323409495 1102 68-69 -2.9493741404477554 1102 70-71 -3.4923317880203406 1102 72-73 0.9202451587325058 1102 74-75 -2.423976032115185 1102 76 -3.0533881098397586 1103 1 -2.0413103044458047 1103 2 -3.1534491969792207 1103 3 -4.095589528516861 1103 4 -2.613662282465242 1103 5 -2.234068167475165 1103 6 -0.3965096800073127 1103 7 1.98802740709311 1103 8 -2.9637291670634482 1103 9 -0.3623716255997955 1103 10-11 -0.34968834507696656 1103 12-13 -1.1083364311896204 1103 14-15 -1.7814739384169727 1103 16-17 2.018557212085703 1103 18-19 1.4838566572226135 1103 20-21 2.340490438704645 1103 22-23 3.5097772855889566 1103 24-25 -1.1133394125088927 1103 26-27 -2.73668510875768 1103 28-29 0.9098888260500217 1103 30-31 -0.4387606614458086 1103 32-33 -2.702892549091324 1103 34-35 0.9196066307409367 1103 36-37 1.7605181875906588 1103 38-39 -2.9830593663008713 1103 40-41 1.4938187560972516 1103 42-43 0.7664767260467471 1103 44-45 -1.1020628614704577 1103 46-47 -0.5984230684425214 1103 48-49 -0.5055017552853407 1103 50-51 -0.03191451589030336 1103 52-53 1.3304124817685015 1103 54-55 -2.2825919845819627 1103 56-57 -0.840666485262787 1103 58-59 1.5441881578241947 1103 60-61 -0.06252938554755616 1103 62-63 -1.6777857421193865 1103 64-65 -0.5984129430963208 1103 66-67 1.8893137500320378 1103 68-69 -1.5922312833048977 1103 70-71 0.9560809103923589 1103 72-73 0.8964356349229874 1103 74-75 0.020468412329258 1103 76 -2.442276998728648 1104 1 2.3965981922862234 1104 2 1.0949168160926739 1104 3 0.04166537344392296 1104 4 1.5530043842014258 1104 5 -1.1131531347954287 1104 6 -0.8540260198765921 1104 7 -2.8093582138219197 1104 8 -2.440853350069986 1104 9 1.9088702044655648 1104 10-11 -1.6192961882142214 1104 12-13 -0.636114208967399 1104 14-15 0.4815979570078639 1104 16-17 2.355158519275246 1104 18-19 -0.8037250421237907 1104 20-21 -0.6513396266548277 1104 22-23 1.4476857823209848 1104 24-25 -0.14928712492719498 1104 26-27 1.624426002353431 1104 28-29 -0.052529474603574045 1104 30-31 0.4615661359398118 1104 32-33 -2.3515853595488387 1104 34-35 -0.6718966372329191 1104 36-37 -1.718239982343981 1104 38-39 -0.11704629440544778 1104 40-41 -0.6025864726609171 1104 42-43 -0.13385007133886972 1104 44-45 -2.157618417026015 1104 46-47 -2.680122414847748 1104 48-49 -0.3372011016905674 1104 50-51 -1.4436792217726584 1104 52-53 -0.40978359666286934 1104 54-55 -1.668212899614641 1104 56-57 -2.0171370734980805 1104 58-59 -1.0620209925026032 1104 60-61 -1.1622025881619358 1104 62-63 -0.7954328009429155 1104 64-65 -1.12128876008979 1104 66-67 -1.2397712172882258 1104 68-69 -0.10693716565783973 1104 70-71 1.2534665313073887 1104 72-73 0.3572199486484777 1104 74-75 0.38484749729657963 1104 76 5.06425894898377 1105 1 -1.6962810646797202 1105 2 0.4664338439564517 1105 3 0.1500245065708583 1105 4 -2.1312061421143653 1105 5 -0.6580447756623009 1105 6 -1.4082055864400616 1105 7 1.1225303310697186 1105 8 -2.431565424373389 1105 9 -2.1313774735530124 1105 10-11 -2.6874076433225795 1105 12-13 -0.3992721037042415 1105 14-15 -1.332643529060249 1105 16-17 -0.4405071153996758 1105 18-19 0.13590344084834527 1105 20-21 0.7542331287631292 1105 22-23 -2.1978250535923287 1105 24-25 0.10303485649695432 1105 26-27 1.571794423406061 1105 28-29 -0.17636848389149762 1105 30-31 -0.5864214801592631 1105 32-33 3.0617273339496123 1105 34-35 1.5804253441912302 1105 36-37 -0.07427713404676695 1105 38-39 0.18945525358217097 1105 40-41 -2.6041344602770167 1105 42-43 -3.033230876292432 1105 44-45 -0.07867104860495999 1105 46-47 -0.23584996902731703 1105 48-49 0.5544397651824973 1105 50-51 -0.37401977904819717 1105 52-53 0.3084826572070938 1105 54-55 -1.437562744815878 1105 56-57 0.4751229884214254 1105 58-59 2.4023752923271218 1105 60-61 -1.2628217832083735 1105 62-63 -0.49357521580359887 1105 64-65 -2.3937345805232226 1105 66-67 1.689021352371217 1105 68-69 2.736716085116157 1105 70-71 -1.0205272767421434 1105 72-73 0.19468124895807293 1105 74-75 1.168129231042709 1105 76 -1.158651267734495 1106 1 -1.8190880822235798 1106 2 -1.7756714192014407 1106 3 -2.29558952851686 1106 4 3.019671050868091 1106 5 -1.95629038969739 1106 6 -2.6853985688962005 1106 7 -0.12308370401800062 1106 8 3.214048610714329 1106 9 -1.9068160700442398 1106 10-11 -0.33302167841030084 1106 12-13 -3.5694475423007326 1106 14-15 -1.6203628273058612 1106 16-17 0.6296683231968139 1106 18-19 -0.6939211205551636 1106 20-21 1.0349348831490914 1106 22-23 1.431999507811181 1106 24-25 0.09777169860221946 1106 26-27 0.9910926690200981 1106 28-29 -0.1623333961722011 1106 30-31 -0.07764955033470144 1106 32-33 -0.44178143798021097 1106 34-35 1.4418288529631624 1106 36-37 1.8660737431462167 1106 38-39 0.6613850781435744 1106 40-41 -0.06729235501385844 1106 42-43 1.2720322816023035 1106 44-45 4.9423815829739866 1106 46-47 0.8904658204463693 1106 48-49 2.866720466936883 1106 50-51 1.068085484109698 1106 52-53 -3.1195875182315014 1106 54-55 0.8729635709735959 1106 56-57 -2.3073331519294555 1106 58-59 0.4164103800464183 1106 60-61 1.4652483922302224 1106 62-63 0.9555475912139464 1106 64-65 -0.3428573875407679 1106 66-67 -0.3162418055235179 1106 68-69 -0.2755646166382313 1106 70-71 5.253548368031602E-4 1106 72-73 1.5297689682563202 1106 74-75 -1.0739760321151834 1106 76 0.879945223493575 1107 1 -4.368107690066719 1107 2 -3.6109655368485 1107 3 -3.311275803026664 1107 4 1.6118279136131903 1107 5 2.0044939240281003 1107 6 -1.5010848434060051 1107 7 -1.8681817432336842 1107 8 3.5003231205182495 1107 9 1.3206349103479162 1107 10-11 1.3218802823740141 1107 12-13 2.9227093204443655 1107 14-15 3.3051273687725704 1107 16-17 2.6198644016281882 1107 18-19 -0.2449015127120262 1107 20-21 -0.5336925678312987 1107 22-23 0.7418034293798073 1107 24-25 -2.73752241904484 1107 26-27 -2.6991034094112756 1107 28-29 3.1533528783376035 1107 30-31 0.4321543712339313 1107 32-33 0.38370875809821925 1107 34-35 -1.5542495784093902 1107 36-37 -0.2182399823439809 1107 38-39 -0.5288110002877993 1107 40-41 -0.13199823736680116 1107 42-43 -0.07502654192710878 1107 44-45 1.548263935915159 1107 46-47 -2.856593003083045 1107 48-49 1.7804459571329616 1107 50-51 0.6151443076391097 1107 52-53 1.2960987562783046 1107 54-55 0.15531651215006548 1107 56-57 -0.01713707349808047 1107 58-59 -0.4443739336790742 1107 60-61 0.9260327059557127 1107 62-63 -1.1483739774135024 1107 64-65 -0.415406407148609 1107 66-67 -1.2397712172882258 1107 68-69 1.0401216578715733 1107 70-71 1.7534665313073887 1107 72-73 1.7689846545308292 1107 74-75 2.326023967884815 1107 76 -1.9357410510162296 1108 1 1.0698008066653095 1108 2 1.0132174696874472 1108 3 -1.0400339729613037 1108 4 0.3307821619792044 1108 5 -2.234068167475165 1108 6 -2.118731902229534 1108 7 4.043582962648667 1108 8 0.5918263884921053 1108 9 4.6376283744002045 1108 10-11 -1.6552439006325237 1108 12-13 0.14166356881037956 1108 14-15 -0.8092517161947512 1108 16-17 1.2685572120857032 1108 18-19 0.9560788794448349 1108 20-21 0.007157105371316419 1108 22-23 1.120888396700071 1108 24-25 -0.3077838569533391 1108 26-27 -0.40335177542434764 1108 28-29 -3.3123333961722032 1108 30-31 2.3667948941097485 1108 32-33 0.7971074509086762 1108 34-35 1.4196066307409367 1108 36-37 2.010518187590659 1108 38-39 -1.9275038107453142 1108 40-41 0.8271520894305837 1108 42-43 -0.8724121628421386 1108 44-45 -0.3798406392482363 1108 46-47 -0.2650897351091892 1108 48-49 -0.8388350886186728 1108 50-51 2.7736410396652538 1108 52-53 2.330412481768498 1108 54-55 0.18963023764026232 1108 56-57 0.38155573695943446 1108 58-59 -0.12247850884246958 1108 60-61 1.2430261700079974 1108 62-63 -1.2333412976749436 1108 64-65 -1.209524054207435 1108 66-67 -0.52735291663463 1108 68-69 0.15776871669510228 1108 70-71 0.06719202150346604 1108 72-73 -0.3535643650770126 1108 74-75 2.492690634551483 1108 76 -1.8311658876175372 1109 1 -2.544578278302012 1109 2 0.4478579925632644 1109 3 2.1593124322674555 1109 4 0.9059455606720128 1109 5 0.35743510049869087 1109 6 -0.7363789610530631 1109 7 -3.3387699785278038 1109 8 0.5003231205182495 1109 9 1.2618113809361517 1109 10-11 1.0277626353151916 1109 12-13 1.3050622616208365 1109 14-15 -0.34193145475683906 1109 16-17 -0.027194421901221233 1109 18-19 0.8433337814056188 1109 20-21 0.8192486086392883 1109 22-23 -0.6699612765025442 1109 24-25 0.586006992719863 1109 26-27 1.3303083552946084 1109 28-29 2.9180587606905455 1109 30-31 0.9321543712339313 1109 32-33 -0.46923241837236773 1109 34-35 -1.1718966372329191 1109 36-37 1.1935247235383706 1109 38-39 -0.7641051179348608 1109 40-41 2.1915311743979053 1109 42-43 1.0720322816023042 1109 44-45 2.312969818268101 1109 46-47 2.672818761622839 1109 48-49 0.4569165453682551 1109 50-51 1.3210266605802836 1109 52-53 1.090216403337127 1109 54-55 -0.5799776054969925 1109 56-57 -0.811254720556903 1109 58-59 1.7320966545562229 1109 60-61 -0.16220258816193578 1109 62-63 0.7928024931747295 1109 64-65 0.790475945792565 1109 66-67 0.6131699591823647 1109 68-69 0.9224745990480443 1109 70-71 1.4887606489544467 1109 72-73 0.3572199486484777 1109 74-75 0.09072985023775715 1109 76 1.2407295372190639 1110 1 5.014245251109752 1110 2 -2.188171419201442 1110 3 1.446077138149807 1110 4 0.9905043842014258 1110 5 -0.8521237230307221 1110 6 1.4437680977704659 1110 7 -0.8314170373513328 1110 8 -0.6026180559523375 1110 9 4.839017263289094 1110 10-11 -1.2351050117436344 1110 12-13 -0.761114208967399 1110 14-15 2.433803839360806 1110 16-17 1.5150849898634817 1110 18-19 3.653995546111503 1110 20-21 0.6703515498157593 1110 22-23 -2.2471671588554862 1110 24-25 -0.627228301397782 1110 26-27 1.561926002353431 1110 28-29 -1.6977500628388675 1110 30-31 -1.1193162170013657 1110 32-33 2.6686352286864548 1110 34-35 -0.7123378137035061 1110 36-37 -0.6005929235204519 1110 38-39 -2.2052815885230928 1110 40-41 0.03895764498614085 1110 42-43 -0.05296771839769576 1110 44-45 0.5923815829739851 1110 46-47 1.321715820446368 1110 48-49 -0.4291128663964514 1110 50-51 -0.6569145158903034 1110 52-53 -2.6487541848981664 1110 54-55 -1.3832864290264055 1110 56-57 -1.934416485262787 1110 58-59 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-0.770231031743684 2116 74-75 -1.7573093654485206 2116 76 3.7799452234935735 2117 1 0.5142452511097524 2117 2 2.4243285807985586 2117 3 -1.2289228618501937 2117 4 3.1530043842014273 2117 5 7.310376276969279 2117 6 10.181268097770467 2117 7 11.343582962648664 2117 8 14.547381944047661 2117 9 12.226517263289097 2117 10-11 6.533644988256366 2117 12-13 9.363885791032601 2117 14-15 3.746303839360806 2117 16-17 5.69633498986348 2117 18-19 5.572745546111502 2117 20-21 6.001601549815756 2117 22-23 2.865332841144511 2117 24-25 3.3977716986022166 2117 26-27 9.124426002353431 2117 28-29 5.370999937161134 2117 30-31 1.7556837829986343 2117 32-33 6.024885228686458 2117 34-35 3.6751621862964967 2117 36-37 6.899407076479548 2117 38-39 2.3947184114769087 2117 40-41 4.032707644986139 2117 42-43 5.7720322816023035 2117 44-45 7.542381582973984 2117 46-47 11.190465820446367 2117 48-49 5.73338713360355 2117 50-51 7.868085484109695 2117 52-53 1.7137458151018308 2117 54-55 0.9729635709735973 2117 56-57 8.859333514737212 2117 58-59 3.349743713379752 2117 60-61 7.531915058896885 2117 62-63 4.3222142578806135 2117 64-65 4.6904759457925636 2117 66-67 0.9837581944764793 2117 68-69 4.557768716695101 2117 70-71 6.600525354836801 2117 72-73 8.46310230158965 2117 74-75 2.4260239678848166 2117 76 4.946611890160241 2118 1 3.7142452511097517 2118 2 2.0243285807985565 2118 3 4.571077138149807 2118 4 -1.4469956157985742 2118 5 2.710376276969278 2118 6 -0.018731902229532693 2118 7 4.943582962648666 2118 8 3.547381944047661 2118 9 -0.773482736710907 2118 10-11 3.9336449882563684 2118 12-13 0.26388579103259957 2118 14-15 -1.253696160639194 2118 16-17 5.19633498986348 2118 18-19 -2.8272544538885 2118 20-21 1.4016015498157586 2118 22-23 3.0653328411445138 2118 24-25 3.997771698602218 2118 26-27 0.5244260023534295 2118 28-29 6.170999937161131 2118 30-31 2.3556837829986357 2118 32-33 -0.5751147713135438 2118 34-35 -1.5248378137035061 2118 36-37 1.1994070764795453 2118 38-39 2.2947184114769072 2118 40-41 0.8327076449861401 2118 42-43 0.8720322816023014 2118 44-45 2.9423815829739866 2118 46-47 2.3904658204463693 2118 48-49 0.43338713360354575 2118 50-51 6.668085484109696 2118 52-53 2.7137458151018308 2118 54-55 2.4729635709735938 2118 56-57 3.8593335147372123 2118 58-59 2.349743713379752 2118 60-61 3.4319150588968874 2118 62-63 0.8222142578806135 2118 64-65 -0.30952405420743645 2118 66-67 1.6837581944764821 2118 68-69 -2.7422312833048963 2118 70-71 6.600525354836805 2118 72-73 1.163102301589653 2118 74-75 0.8260239678848151 2118 76 2.34661189016024 2119 1 0.5142452511097524 2119 2 7.95766191413189 2119 3 2.571077138149807 2119 4 11.886337717534762 2119 5 10.377042943635946 2119 6 5.047934764437134 2119 7 4.876916295981999 2119 8 6.480715277380995 2119 9 6.026517263289094 2119 10-11 3.7336449882563656 2119 12-13 10.863885791032601 2119 14-15 10.41297050602747 2119 16-17 7.129668323196817 2119 18-19 8.372745546111503 2119 20-21 -0.6317317835175764 2119 22-23 11.231999507811178 2119 24-25 14.66443836526889 2119 26-27 7.957759335686767 2119 28-29 6.937666603827797 2119 30-31 6.9223504496652986 2119 32-33 4.324885228686455 2119 34-35 7.975162186296494 2119 36-37 7.399407076479548 2119 38-39 7.794718411476907 2119 40-41 7.799374311652805 2119 42-43 6.7386989482689685 2119 44-45 -2.8242850836926827 2119 46-47 7.457132487113032 2119 48-49 7.800053800270213 2119 50-51 4.301418817443029 2119 52-53 8.24707914843517 2119 54-55 3.1062969043069266 2119 56-57 9.326000181403877 2119 58-59 9.01641038004642 2119 60-61 7.965248392230219 2119 62-63 9.488880924547278 2119 64-65 11.790475945792565 2119 66-67 9.750424861143149 2119 68-69 4.157768716695102 2119 70-71 7.567192021503466 2119 72-73 3.3964356349229874 2119 74-75 11.326023967884815 2119 76 6.2799452234935735 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 8.0 13 93.0 14 204.0 15 346.0 16 464.0 17 474.0 18 513.0 19 506.0 20 374.0 21 268.0 22 212.0 23 153.0 24 87.0 25 80.0 26 46.0 27 37.0 28 34.0 29 39.0 30 43.0 31 25.0 32 44.0 33 42.0 34 54.0 35 81.0 36 84.0 37 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.172413793103448 28.52873563218391 8.505747126436782 53.79310344827586 2 3.613080265264121 45.62085524811342 24.30825520237823 26.457809284244227 3 5.168076835124628 28.79030413903499 19.1401783672536 46.901440658586786 4 4.321975760347588 28.081408643951523 31.214269380288133 36.38234621541276 5 4.047564600960439 31.420077749828497 36.08506745941001 28.44729018980105 6 6.70020580836954 31.077063800594555 27.98993825748914 34.232792133546766 7 16.80768351246284 34.118454150468786 16.00731763091699 33.06654470615138 8 6.723073404985136 34.25565973016236 26.846558426709354 32.17470843814315 9 11.227989938257489 27.258175165790078 17.425108621083922 44.08872627486851 10-11 8.689686713926365 29.453464440887263 27.864166476103364 33.99268236908301 12-13 7.946489823919506 27.32677795563686 25.165790075463068 39.56094214498056 14-15 12.87445689458038 30.43677109535788 20.546535559112737 36.14223645094901 16-17 17.139263663388977 29.84221358335239 19.6890006860279 33.329522067230734 18-19 13.446146809970273 27.589755316716214 18.44271667047793 40.521381202835585 20-21 10.804939400868967 28.710267550880403 20.58083695403613 39.9039560942145 22-23 18.900068602789847 29.659272810427623 16.796249714155042 34.64440887262749 24-25 15.389892522295906 34.36999771324034 15.652869883375256 34.5872398810885 26-27 11.205122341641893 34.850217242167844 21.43837182712097 32.506288589069285 28-29 15.995883832609191 29.922250171506974 24.23965241253144 29.84221358335239 30-31 15.389892522295906 29.430596844271665 25.165790075463068 30.013720557969357 32-33 10.564829636405214 36.027898467871026 22.741824834209925 30.665447061513834 34-35 10.290418477018065 36.873999542648065 20.192087811571007 32.643494168762864 36-37 12.748685113194602 30.25383032243311 22.718957237594328 34.278527326777954 38-39 13.480448204893666 25.12005488223188 27.441115938714837 33.95838097415961 40-41 10.656300022867597 28.252915618568487 24.66270294991996 36.428081408643955 42-43 6.7116396066773385 25.005716899153903 29.510633432426253 38.77201006174251 44-45 8.186599588383261 25.37159844500343 32.22044363137434 34.22135833523897 46-47 7.946489823919506 31.237136976903727 27.681225703178598 33.13514749599817 48-49 8.220900983306654 34.049851360622 21.484107020352162 36.24514063571919 50-51 7.489137891607592 32.83786873999542 21.083924079579237 38.58906928881775 52-53 7.008918362680082 33.46672764692431 21.907157557740682 37.61719643265493 54-55 6.963183169448891 30.939858220900984 20.935284701577864 41.16167390807226 56-57 11.182254745026297 31.500114337983078 20.306425794648984 37.011205122341636 58-59 10.004573519323118 30.814086439515208 19.700434484335698 39.48090555682598 60-61 8.198033386691058 27.53258632517722 20.340727189572373 43.92865309855934 62-63 11.571003887491425 28.13857763549051 20.42076377772696 39.869654699291104 64-65 11.308026526412075 29.922250171506974 15.607134690144067 43.16258861193688 66-67 8.015092613766294 36.73679396295449 13.19460324719872 42.05351017608049 68-69 11.125085753487308 34.86165104047564 14.486622455979878 39.52664075005717 70-71 10.919277383946948 35.03315801509261 16.098788017379373 37.948776583581065 72-73 9.547221587011204 36.565286988337526 13.217470843814317 40.670020580836955 74-75 12.108392407957924 33.54676423507889 13.66338897781843 40.681454379144746 76 10.153212897324492 36.49668419849073 13.034530070889549 40.31557283329522 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.0 18 0.5 19 1.5 20 4.0 21 6.0 22 5.5 23 7.5 24 10.0 25 10.0 26 10.5 27 16.5 28 26.0 29 30.0 30 35.5 31 46.5 32 57.0 33 62.0 34 74.0 35 99.0 36 124.0 37 136.0 38 142.5 39 157.0 40 190.5 41 229.5 42 243.0 43 240.5 44 232.0 45 229.0 46 232.0 47 235.5 48 252.0 49 261.0 50 257.0 51 248.5 52 230.5 53 199.5 54 178.0 55 163.0 56 152.5 57 129.5 58 102.0 59 102.0 60 92.0 61 77.0 62 72.0 63 73.5 64 57.5 65 37.5 66 32.5 67 30.0 68 24.0 69 16.5 70 12.5 71 10.0 72 10.0 73 6.5 74 3.0 75 3.0 76 1.5 77 2.0 78 3.0 79 2.0 80 1.5 81 0.5 82 0.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5259547221587012 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 4373.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.05625428767436 #Duplication Level Percentage of deduplicated Percentage of total 1 98.55410447761194 96.63846329750743 2 1.0494402985074627 2.058083695403613 3 0.2798507462686567 0.8232334781614453 4 0.09328358208955223 0.3658815458495312 5 0.023320895522388058 0.11433798307797849 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACTTCTGCCTTGGCCTCCCAAAGTGCTGTGATTATAGGCTGTCTCTTAT 5 0.11433798307797849 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0228675966155957 0.0 11 0.0 0.0 0.0 0.0228675966155957 0.0 12 0.0 0.0 0.0 0.0457351932311914 0.0 13 0.0 0.0 0.0 0.0686027898467871 0.0 14 0.0 0.0 0.0 0.0686027898467871 0.0 15 0.0 0.0 0.0 0.0686027898467871 0.0 16 0.0 0.0 0.0 0.0686027898467871 0.0 17 0.0 0.0 0.0 0.0686027898467871 0.0 18 0.0 0.0 0.0 0.1829407729247656 0.0 19 0.0 0.0 0.0 0.3658815458495312 0.0 20 0.0 0.0 0.0 0.4116167390807226 0.0 21 0.0 0.0 0.0 0.43448433569631834 0.0 22 0.0 0.0 0.0 0.43448433569631834 0.0 23 0.0 0.0 0.0 0.43448433569631834 0.0 24 0.0 0.0 0.0 0.48021952892750974 0.0 25 0.0 0.0 0.0 0.48021952892750974 0.0 26 0.0 0.0 0.0 0.48021952892750974 0.0 27 0.0 0.0 0.0 0.5259547221587011 0.0 28 0.0 0.0 0.0 0.5259547221587011 0.0 29 0.0 0.0 0.0 0.5259547221587011 0.0 30 0.0 0.0 0.0 0.5259547221587011 0.0 31 0.0 0.0 0.0 0.5716899153898926 0.0 32 0.0 0.0 0.0 0.5945575120054882 0.0 33 0.0 0.0 0.0 0.6631603018522754 0.0 34 0.0 0.0 0.0 0.7774982849302539 0.0 35 0.0 0.0 0.0 0.8918362680082323 0.0 36 0.0 0.0 0.0 0.9604390578550195 0.0 37 0.0 0.0 0.0 1.2805854104733592 0.0 38 0.0 0.0 0.0 1.5549965698605077 0.0 39 0.0 0.0 0.0 1.6464669563228904 0.0 40 0.0 0.0 0.0 2.0123485021724217 0.0 41 0.0 0.0 0.0 2.4697004344843356 0.0 42 0.0 0.0 0.0 2.4925680310999314 0.0 43 0.0 0.0 0.0 2.6526412074091015 0.0 44 0.0 0.0 0.0 2.9499199634118454 0.0 45 0.0 0.0 0.0 3.498742282186142 0.0 46 0.0 0.0 0.0 3.7045506517265037 0.0 47 0.0 0.0 0.0 4.024697004344843 0.0 48 0.0 0.0 0.0 4.1161673908072265 0.0 49 0.0 0.0 0.0 4.321975760347588 0.0 50 0.0 0.0 0.0 4.390578550194374 0.0 51 0.0 0.0 0.0 4.504916533272353 0.0 52 0.0 0.0 0.0 4.73359249942831 0.0 53 0.0 0.0 0.0 4.939400868968671 0.0 54 0.0 0.0 0.0 5.030871255431054 0.0 55 0.0 0.0 0.0 5.190944431740224 0.0 56 0.0 0.0 0.0 5.419620397896181 0.0 57 0.0 0.0 0.0 5.53395838097416 0.0 58 0.0 0.0 0.0 5.762634347130117 0.0 59 0.0 0.0 0.0 5.945575120054882 0.0 60 0.0 0.0 0.0 6.174251086210839 0.0 61 0.0 0.0 0.0 6.471529842213584 0.0 62 0.0 0.0 0.0 6.631603018522753 0.0 63 0.0 0.0 0.0 6.768808598216327 0.0 64 0.0 0.0 0.0 6.883146581294306 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE