FastQCFastQC Report
Wed 25 May 2016
SRR1780548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT126828.99611250857535TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT46910.724902812714383TruSeq Adapter, Index 5 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1633.7274182483420994TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1072.44683283786874RNA PCR Primer, Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC972.2181568717127833RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT882.0123485021724217No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT551.2577178138577636No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC461.0519094443174024TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT451.0290418477018064No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT441.0061742510862108RNA PCR Primer, Index 5 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGTCTT230.5259547221587012Illumina PCR Primer Index 5 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG220.5030871255431054TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGT210.48021952892750974No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGTCTT190.43448433569631834Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGCGACACAAAGTGATCTCGTATGCCGT190.43448433569631834Illumina PCR Primer Index 5 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC180.41161673908072266RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAAAAGTGATCTCGTATGCCGT180.41161673908072266No Hit
CTTATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGT150.3430139492339355No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT140.3201463526183398No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGTCTT140.3201463526183398Illumina PCR Primer Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCT140.3201463526183398RNA PCR Primer, Index 5 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCC130.2972787560027441No Hit
CTTATAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG130.2972787560027441TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCAAAGTGATCTCGTATGCCGT130.2972787560027441Illumina PCR Primer Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG120.27441115938714844No Hit
CTTATACACATCTCCGAGCCCAAGAGACACAAAGTGATCTCGTATGCCGT110.2515435627715527Illumina PCR Primer Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGCTCTCGTATGCCGT110.2515435627715527No Hit
CTTATACACATCTCCGAGCCCACGAGCCACAAAGTGATCTCGTATGCCGT100.22867596615595698TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGTCTT100.22867596615595698Illumina PCR Primer Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCG100.22867596615595698No Hit
CTTATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT100.22867596615595698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCC90.20580836954036133No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT90.20580836954036133No Hit
ATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTGCTT90.20580836954036133TruSeq Adapter, Index 5 (96% over 32bp)
CTTAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC90.20580836954036133TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGTGACACAAAGTGATCTCGTATGCCGT90.20580836954036133Illumina PCR Primer Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCACACGAGACACAAAGTGATCTCGTATGCCGT80.1829407729247656Illumina PCR Primer Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT80.1829407729247656No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCG80.1829407729247656No Hit
ATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGTCTT70.1600731763091699Illumina PCR Primer Index 5 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT60.13720557969357422TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG60.13720557969357422No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCC60.13720557969357422No Hit
CTTATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGT60.13720557969357422Illumina PCR Primer Index 5 (96% over 26bp)
CTTATCACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC60.13720557969357422TruSeq Adapter, Index 5 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCCT60.13720557969357422TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATATCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT60.13720557969357422RNA PCR Primer, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGC60.13720557969357422TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGACCACGAGACACAAAGTGATCTCGTATGCCGT60.13720557969357422Illumina PCR Primer Index 5 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG60.13720557969357422TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC60.13720557969357422TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT60.13720557969357422No Hit
CTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTGCTTGA60.13720557969357422RNA PCR Primer, Index 5 (96% over 33bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTCTGCCGT60.13720557969357422No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACATCGTATGCCGT60.13720557969357422No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC50.11433798307797849No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGCTCTCGTATGCCGTCTT50.11433798307797849Illumina PCR Primer Index 5 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT50.11433798307797849No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAAATCGTATGCCGT50.11433798307797849No Hit
CTTATACACATCTCCGAGCCCACGCGACACAAAGTGACCTCGTATGCCGT50.11433798307797849No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGCTATCGTATGCCGT50.11433798307797849No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGTCT50.11433798307797849No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT50.11433798307797849No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCG50.11433798307797849No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGTCTT50.11433798307797849Illumina PCR Primer Index 5 (95% over 21bp)
CCTATAATCACAGCACTTTGGGAGGCCAAGGCAGAAGTACTGTCTCTTAT50.11433798307797849No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA150.002078460870.038
ATCTAGT150.002078460870.037
TGATCTA150.002078460870.035
AGTATGC150.002078460870.041
TAGTATG150.002078460870.040
GATCTAG150.002078460870.036
CTAGTAT150.002078460870.039
GATATCG252.1352177E-455.99999636
TATCGTA252.1352177E-455.99999638
TGATATC252.1352177E-455.99999635
AGTGATA252.1352177E-455.99999633
GTGATAT252.1352177E-455.99999634
ATATCGT252.1352177E-455.99999637
AAAATAA200.00649780752.50000468
ATCGTAT351.7617058E-550.039
TTATACA2950.039.1525422
CTTATAC2950.039.1525421
TATACAC3100.038.3870963
TCTTCTG3450.034.49275250
CTTCTGC3450.034.49275251
Produced by FastQC (version 0.11.5)