FastQCFastQC Report
Wed 25 May 2016
SRR1780547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGCGCACCTCCGCGGTGTCCACGCTCGGGGTGCGGCTGCTCGCGGA11.0No Hit
GCGCCGGAGGCCTCGTCGTCGGAGCCGGCGACGTCGCCGTCGCCGCCGCG11.0No Hit
GGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAA11.0No Hit
GGCCAGCTCGGCGACGTCCGGGTTCTTCTTCTGCGGCATGATCGAGGACC11.0No Hit
TAGACACCTCTGTCGCGACCGACGGGCACGGCCGTAGAGAACTCGCGGGT11.0No Hit
GGTCTCCGGCGGGCAGGCGCAGCGGGTGGCCGTGTGCCGCGCCCTCGTGA11.0No Hit
TAGACTTCAACTACAACAAACTGTCCGAATCAAAGGAAGAGAGACGGACA11.0No Hit
TGGGTGGGTAGCGCGAGGATTTTCGGGTCAGCGGTGGTGGAGAAGCTGTC11.0No Hit
ATGGTGGTGTACTCCAGAGCGCCGTGCTCCTCCAGCGTCTGTCTCTTATA11.0No Hit
ATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCT11.0No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG11.0No Hit
GCAGGGAGCGCACCGCCAGGTCCGAGCGGTCCGCCGCTGTCTCTTATACA11.0No Hit
GATCACGGAGATCGTCTCGGTGCGCTCCGAGGTGGACCACGCGCTGCTGC11.0No Hit
ATCTCCTCCACGCCTGCTGCGTGGCCCTGCGGCGCGACGAGAGAAGAAAA11.0No Hit
ACACCAGCCTAACCAGATTTCAAATTTTATCTTTTGGCGGTATGCACTTT11.0No Hit
CATCTGCACCGATCTGCCGGCAGAACTCAGCCAGCAGGCTGTCTCTTATA11.0No Hit
ACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTCGA11.0No Hit
GTCGAGGCGCACACCGGAGCCGCCGGCCGAGCGGTAGGCGGGGGTGGTTC11.0No Hit
GGCGGGCACCTGGCGAGAAAGGACGGCAGGCCGGGCGTGGAGTTTGTTTT11.0No Hit
GTTCTACCGGTGCGCGGATAGGCCCCATCCTCCGTTTCCCATATCGCCCT11.0No Hit
CCACTGGCGCTGCCGCTCGGCGACGACGTCCGCGGCCTTGCGGATAGCTG11.0No Hit
CTATACACATCCGTCGTGGACGACGACCAAGGCCCGGGGCGGGGTGTGTT11.0No Hit
ACTTTAAAGTTCATATGGAACCAAAAAAGAGCCCGTATCACCAAGTCAAT11.0No Hit
GTTCACTCCTGTCCCTGCAGGCTCTTTTGGTTCCCGTTGTCAGTCTCCTT11.0No Hit
CTCACCGACGGCGACGGCGCCCTCACCGGCCCCGTGGCCCGCGGCGACGT11.0No Hit
GGCGGTGGGGGCTTCGACATGGGCTTTAGGGAGGCATAAGTCCTGTCTCT11.0No Hit
GGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAG11.0No Hit
TGCAGGGACAGGGCGAACGTCAGCGGCACCCCCTCCTCGGCCAGCTTCCG11.0No Hit
GCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAAC11.0No Hit
TCGTGGGGCTGCAGGGGCGCGGCTCCGGCCCGGGCCTGGCGCTTGCTCTT11.0No Hit
CACAATAATGAGGGTAGACAACCGATATTCTACAGGGTTACTGTGTTGCT11.0No Hit
CGTCCACGTCCTCCCGATCCGGCAGCGCCACCCGCACCAGCGGCTCACGC11.0No Hit
GGAGGCCGCCGTGCGCGAGGCCCTGGGCGAGGGCGGGCTGGCCAAGGCCT11.0No Hit
ACTAACAAGAGCATAATATAAAGCCGTATTAAGTACACATGAAACATTCT11.0No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG11.0No Hit
TCCCGGGCGGAGGCGTTGGCGATCCGTGCGGCCTTCTCCTCGCCTGTCTC11.0No Hit
TCCCCAGCCTGCGGGCTAGCGGCGGCAGGCTAACACGCCGACCCTAGTTC11.0No Hit
ATCATCGGGCAGACTTATGTCTCGGATCCTGATTACTAGGGGGGATGCCA11.0No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCCTGTCTCTTAT11.0No Hit
ATCCGCACCGCCGCCGTGGGCTCCCTCGTCAGCCTGTCTCTTATACACAT11.0No Hit
GGCCTGCGCCGGGAGCCGGGCGAACCGGAGGCCGCCCTCGGTGGGGGCTG11.0No Hit
ATACACATCTGACGCCGCCGACGAACTGCTAGGTGTAGATCTCGGTGGTC11.0No Hit
GACCCAGCCATCCCATTACTGGGTATATACCCAAAGGATTATAAATCATG11.0No Hit
ACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA11.0No Hit
GTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCGGGAGTACTA11.0No Hit
GATTGTCTTCGCACACCTACACCGCTGTTGAGCGCATGCCTCGTACCGCC11.0No Hit
GCACCAGGGGCCGCCGTTTCCCGAACGAAAGCGCGCCGGGGGCCGCTGGT11.0No Hit
ATGATGATTAGCCTGAGCTCCTTGACGGTGGGCTCCAGGCTACGTGAATC11.0No Hit
GGTTAAATACAGACCAATAGCCTTCACCGCCCTCAGTAAGTTGCAATCCT11.0No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA11.0No Hit
AGCCAACCCCATGGCCTCCATGACTTTTTCAAAAAGGTATTAGACTGTCT11.0No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT11.0No Hit
AGTCATATAACACTAGTGGAAAATTTGTCTCAAGAGGTTCTTTACACATT11.0No Hit
ACCACAGCCCCCGGCACCCCTGGCGGCGAGTGCTGGACGCAATCCGGCCG11.0No Hit
GATAGACTCCGTGGGGCCGGCTCCAGCAGCGCCTGGGGGGCTGCCTGGCT11.0No Hit
CGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATG11.0No Hit
GTGCAGCACGCCGTCCGCGTCGGGGACGACGGTGGCGCTGACCCCGGCGG11.0No Hit
ACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTT11.0No Hit
GGAGCCCCTCGGCCCAGGCGCGGACGGAGGCCGGAGTCTGTCTCTTATAC11.0No Hit
CCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCTGTCTC11.0No Hit
CGGGATCTTCGCGTCGGTCGAGCAACACGAACCTGACGTTCCCTGGACCC11.0No Hit
GGTCTCAGAGCCTGCCTTCTTGCAGCTGTCAGGGTACATTCAGGACTCTC11.0No Hit
TCGGGGGTGAGCCCCGCGTCCTCGGCCCAGGCGCGCAGCTGTCTCTTATA11.0No Hit
TGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAAGGTTTTC11.0No Hit
CGGGCGGGCAGGCCTCGCCGCCGAAGATCTGTCTCTTATACACATCTGAC11.0No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACCTGTCTCTT11.0No Hit
CCTGCGCCGCGAGTACCCGCAGACCTGGGTGTTCGCCGTCTGTCTCTTAT11.0No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG11.0No Hit
GTTCACCAAGAAGGAGCTGCTGCTGCTCAACCGTGAGCCTGTCTCTTATA11.0No Hit
GGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCAT11.0No Hit
TTCTTCTTGAGAGGTTTCGTTCCAGCCTTTTAAAGGGAAATGTAATGATA11.0No Hit
ACGTCAGGTCGTCCACGCGGGTGGGCGCGGTGCTCGGGACCTGTCTCTTA11.0No Hit
CTTGCGGGCCATGTCCGGGACACGGTCCGGGATGCGGTCCGCGGGCCTGT11.0No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT11.0No Hit
GTAATACAATTATCAAAAATCAAAGACAAAAATTCCGAGAGCAGCAAGAG11.0No Hit
ACCAGAGCAAAGGGGCCAGGGGACAAGGTCTAACGCCGAGACGACCGCGG11.0No Hit
CTCGAGTGATGTGGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAG11.0No Hit
GGGAAAACCACACAATACATTGACCACTCATTTTATAGTTTAGAGCTAGA11.0No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT11.0No Hit
GGCCTGGACGACGTCGAAGGCGTGGCCGCAGCTGTCTCTTATACACATCT11.0No Hit
CTCATCCAGGACGCGGCCGCGGACTACGAGTCCCGCGCGATGGTCAGCTC11.0No Hit
ACCCGGTGCCGGGCGACCTCCACCGGCCCCTGCGCACCGGCGTCTGTCTC11.0No Hit
GTTCGGGACGCTGCTGCTCACGGCCGCGGAACTGGATCTGTCTCTTATAC11.0No Hit
GCCTTACCCCCCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAAC11.0No Hit
GCGTGACGCTGGTCCCCTGCACGTAGACGAGTCCGCCCGTCGCCGTGTCG11.0No Hit
CTTCCGCTCGTTGCGCTCTACGTCCGCTACCTCCAGGGACTGTCTCTTAT11.0No Hit
GCCTGAGAGCAGCCTCGACGGCGTGAGGCTCCAGGGCCTCTTCTGGATGG11.0No Hit
AACCTGGACGCCGCGCACTCGGACGTCGACTTCGTCTGTCTCTTATACAC11.0No Hit
GCCGTGTGGTGGGCGACGACGTCGCCATCATGATCTGTCTCTTATACACA11.0No Hit
GGGTTGCGGACCGAGGCAGGGGCGGGGTTACCGCCCTGTGGCGTTTAGCC11.0No Hit
GGCTACCTCTCCGCGAACCGCGACCTGATCCTGCTGGCCGGCCGCCGTGT11.0No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCCTGTCTC11.0No Hit
CGCTGATGGAGGCCGAGCTGGACCGGATGGCCGAGGCCGACGTCACCGGG11.0No Hit
CATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCC11.0No Hit
GGCGTGGACCGCGCCGCCGACGGCGAGCTCACCGTCCAGGGCCCTGTCTC11.0No Hit
TTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTC11.0No Hit
CCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACA11.0No Hit
CATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTG11.0No Hit
GTGATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTAC11.0No Hit
GCACAAACACTGTCTCTTATACACATCTGACGCTGCCGACGAGCCGGTCG11.0No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers