FastQCFastQC Report
Wed 25 May 2016
SRR1780547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCGCAAGGCCGCGGACGTCGTCGCCGAGCGGCAGCGCCAGTGGCTG11.0No Hit
GGAGAGTCCTGAATGTACCCTGACAGCTGCAAGAAGGCAGGCTCTGAGAC11.0No Hit
GTTCACGAGGGCGCGGCACACGGCCACCCGCTGCGCCTGCCCGCCGGAGA11.0No Hit
TGTTTGTGCCTGTCCTTATACACATCTCCGAGCCCACGAGCTAACAACAA11.0No Hit
CTCACGTACCGCTCTTCAGGGACAGTGGAGTTGGCCCCGATGACGGGGAC11.0No Hit
AAGTCAGGTAGCGTGATGCCTCCAGCTTTCTTCTTTGGCTTAGGATTGAC11.0No Hit
CTCAAGGACCTCGAGGAGGACGAGACCCTCCTGGTGCAGTCCGGCAAGCC11.0No Hit
ATCTCCGAGCCCACGAACGATCCAAAATCTCGTATGCCGTCTTCTGCTTG11.0TruSeq Adapter, Index 6 (96% over 29bp)
CTATGGCTGAGGGGAGTCAGGGGTGGAGACCTAATTGGGCTGATTTGCCT11.0No Hit
ACGCCGGTGCGCAGGGGCCGGTGGAGGACGCCCGGCACCGGGTCTGTCTC11.0No Hit
GTGTCGACGTCGCCGCGGGCCACGGGGCCGGTGAGGGCGCCGTCGCCGTC11.0No Hit
GGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCT11.0No Hit
GCCCAGCTCGGCTCGAATAAGGAGGCTTAGAGCTGTGCCTAGGCTGTCTC11.0No Hit
GATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGCTGTCTCTTAT11.0No Hit
ACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGG11.0No Hit
CCCCGGTGACGTCGGCCTCGGCCATCCGGTCCAGCTCGGCCTCCATCAGC11.0No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG11.0No Hit
ATCATGATGGCGACGTCGTCGCCCACCACACGGCCTGTCTCTTATACACA11.0No Hit
CCCACCCGGTCACCGCCGAGGACCGCGCCTTCCTGGCCGCCGCCGGGGTC11.0No Hit
CTTATACACACTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC11.0No Hit
TCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCATGAGCC11.0No Hit
ACGCTGGAGGAGCACGGCGCTCTGGAGTACACCACCATCTGTCTCTTATA11.0No Hit
CGCGGTCACGCCCAGCTCCGCTTCGATGCGCGCGGCCAGGGCGGCGGCCC11.0No Hit
CGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAG11.0No Hit
TACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAACCCCTG11.0No Hit
CTCCACAGGGTCCTCGATCATGCCGCAGAAGAAGAACCCGGACGTCGCCG11.0No Hit
CTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAAC11.0No Hit
ACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCT11.0No Hit
CGGCGGACCGCTCGGACCTGGCGGTGCGCTCCCTGCCTGTCTCTTATACA11.0No Hit
AGTATGTTGAGTCCTGTAAGTAGGAGAGTGATATTTGATCAGGAGAACGT11.0No Hit
CAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATACACATCTCCG11.0No Hit
GCTGACGAGGGAGCCCACGGCGGCGGTGCGGATCTGTCTCTTATACACAT11.0No Hit
GGTACTCCTTTGTATACGAAGTGTTTCTTATCTCTTGCTGCTCTCGGAAT11.0No Hit
GACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCCCTGTCTCT11.0No Hit
GCCCGCGGCGGCGACGGCGACGTCGCCGGCTCCGACGACGAGGCCTCCGG11.0No Hit
ACCCGTGCCGCCGTCGCCCGGCCCCGCCACCCCGGACGCCGGGCGCTGTC11.0No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT11.0No Hit
CTGTGGGCCTGGTGCCGGCCATCCGGAAGCTGGCCGAGGAGGGGGTGCCG11.0No Hit
ACGAAGTCGACGTCCGAGTGCGCGGCGTCCAGGTTCTGTCTCTTATACAC11.0No Hit
GTCCCGAGCACCGCGCCCACCCGCGTGGACGACCTGACGTCTGTCTCTTA11.0No Hit
CAGTGGGGACCTACTAGAAAAAGGAACTGGCATGCACACGTGCCCAGCAG11.0No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACCTGTCTCTT11.0No Hit
CAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTC11.0No Hit
GGCCCTGGACGGTGAGCTCGCCGTCGGCGGCGCGGTCCACGCCCTGTCTC11.0No Hit
TTCTCTAACTGTGCAGCTGCAAGGTCACTAGACAGGATAAACTCAAGTCC11.0No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA11.0No Hit
AAATTATAACAAACTCTCTCTCCGAGCCCACGAGACGGTAAACCATCTCG11.0No Hit
CCTGCTGGCTGAGTTCTGCCGGCAGATCGGTGCAGATGCTGTCTCTTATA11.0No Hit
CTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGG11.0No Hit
ACTCGAGACCGCCGGCCAGGCGCTGCGGGAGCCGGGCGAGGGGTGTCTGT11.0No Hit
GCGAGGAGAAGGCCGCACGGATCGCCAACGCCTCCGCCCGGGACTGTCTC11.0No Hit
CGCCGATGACCAGCACGAGACCCTTGGGCCGCGGCCTGACCTCCGCGCTC11.0No Hit
GGCCCACACGGCGGCCGGCCAGCAGGATCAGGTCGCGGTTCGCGGAGAGG11.0No Hit
CCCTCACCGAGGGCGGCGTCCGGTTCGACCGGCTCACCGCGCCCGCCCTG11.0No Hit
GTAATAAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGT11.0No Hit
CTTATACACATCTCCGAGCCCACGACTTGACCCTATCTCGTATGCCGTCT11.0No Hit
CATAAACTGTGGGGGGTGTCTTTGGGGTTTGGTTGGTTCGGGGTATGGGG11.0No Hit
TCCCTGGAGGTAGCGGACGTAGAGCGCAACGAGCGGAAGCTGTCTCTTAT11.0No Hit
GCGATGAGTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGT11.0No Hit
GGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATG11.0No Hit
ACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCAC11.0No Hit
AATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATT11.0No Hit
ACTCCGGCCTCCGTCCGCGCCTGGGCCGAGGGGCTCCCTGTCTCTTATAC11.0No Hit
GCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGC11.0No Hit
GTCCAGGTGGGCATCGACACGGCGACGGGCGGACTCGTCTACGTGCAGGG11.0No Hit
GTATGGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGGACT11.0No Hit
CTGCGCGCCTGGGCCGAGGACGCGGGGCTCACCCCCGACTGTCTCTTATA11.0No Hit
CGGCCGGACCGCGCGCAGTAGACGGCGTCCGGCGCGTTCAGCCAGATCTC11.0No Hit
GCTCACGGTTGAGCAGCAGCAGCTCCTTCTTGGTGAACCTGTCTCTTATA11.0No Hit
GGTTAGTTGTGGCAATAAAAATGATTAAGGATACTAGTATAAGCTGTCTC11.0No Hit
CTTCTCCACCACCGCTGACCCGAAAATCCTCGCGCTACCCACCCACTGTC11.0No Hit
GGCGTGAGCCGCTGGTGCGGGTGGCGCTGCCGGATCGGGAGGACGTGGAC11.0No Hit
TTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGC11.0No Hit
TGTTAGAGAAGCCAGTATTTTGAGCAACTTTTAGGTTCCTAAGCACCATC11.0No Hit
ATCCAGTTCCGCGGCCGTGAGCAGCAGCGTCCCGAACCTGTCTCTTATAC11.0No Hit
ACACACAACTCTCCCTCCGAGCCCACGAGACTAACAACAATCTCGTATGC11.0No Hit
AGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.0TruSeq Adapter, Index 25 (96% over 29bp)
GCCCGCGGACCGCATCCCGGACCGTGTCCCGGACATGGCCCGCAAGCTGT11.0No Hit
CCAATATATCATTTATCCTACAGAATGTTTCATGTGTACTTAATACGGCT11.0No Hit
CCATTGAGCCACTCTACGTGCCATGCACCATATGAGAGAGCAAGACACAA11.0No Hit
CCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.0Illumina PCR Primer Index 1 (96% over 27bp)
ACGGCGAACACCCAGGTCTGCGGGTACTCGCGGCGCAGGCTGTCTCTTAT11.0No Hit
CTGCGGCCACGCCTTCGACGTCGTCCAGGCCCTGTCTCTTATACACATCT11.0No Hit
TACCATGAGCCCTACAAACAACTAACCTGCCACTAATAGTTATGTCATCC11.0No Hit
ATCTTCGGCGGCGAGGCCTGCCCGCCCGCTGTCTCTTATACACATCTCCG11.0No Hit
CTCTTATACACATCTCCGAGACCACGAGACTGGTCACAATCTCGTATGCC11.0No Hit
TCTTATACACATCTCCGACCCACGAGACTAACAACAATCTCGTATGCCGT11.0No Hit
CTCTTATACACATCTCTGAGCCCACGAGACTAACAACAATCTCGTATGCC11.0No Hit
CCCCGGCGTGTTCGACGCCCACGTGGAGGGCTCCGAGCCCACGAGACATG11.0No Hit
ACCTATAGTAGGTGGCCACCCTGGTTGTGCTCATTCCTGGCACAGGCCTC11.0No Hit
TCTAATACCTTTTTGAAAAAGTCATGGAGGCCATGGGGTTGGCTCTGTCT11.0No Hit
GTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATAT11.0No Hit
CGCCCGGACACCGGCACCATCACCGCCTACCGCTCGGCCGGCGGCCCCTG11.0No Hit
CTCTACCAGCGGCCCCCGGCGCGCTTTCGTTCGGGAAACGGCGGCCCCTG11.0No Hit
GTGCCACCGCCTCGAGTGCACCTACAACGCCGACCTCGCCTGCACCGCCG11.0No Hit
GGTGATGTTTTTGGTAAACAGGCGGGGGAAGATTTTTCGAGTTTCTTTTT11.0No Hit
ACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAA11.0No Hit
CTATATGATAGGCATGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGT11.0No Hit
CCGCAGGACTATTCCTAGCCATACACTACTCACCAGACGCCTCAACCGCC11.0No Hit
GCCTTGGCCAGCCCGCCCTCGCCCAGGGCCTCGCGCACGGCGGCCACCCT11.0No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers