FastQCFastQC Report
Wed 25 May 2016
SRR1780546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTC40.22026431718061676No Hit
TCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACGAAACG30.16519823788546256No Hit
TGCTCACCCTGATCAGAGGATTGAGTAAACGGCTAGGCTAGAGGTGGCTA30.16519823788546256No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA30.16519823788546256No Hit
GCGTTTAGAAGGGCTATTTGTTGTGGGTCTCATGAGTTGGAGTGTAGGAT30.16519823788546256No Hit
ATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAA30.16519823788546256No Hit
CACTAAAACACTCACCAAGACCTCAACCCCTGACCCCCATGCCTCAGGAT30.16519823788546256No Hit
GATAGTAATAGGGCAAGGACGCCTCCTAGCTTGTTAGGGACGGATCGGAG30.16519823788546256No Hit
CAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTA30.16519823788546256No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC30.16519823788546256No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATCTGTCTCT30.16519823788546256No Hit
GCTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACAC30.16519823788546256No Hit
CTGAAGTACCGTGAGGTTCCTGATGTGCGGGCGGTAGACGGTCTGTCTCT30.16519823788546256No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT30.16519823788546256No Hit
TAATTACTGTGGCCCCTCAGAATGATATTTGGCCTCACGGGAGGACATAG30.16519823788546256No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTCT30.16519823788546256No Hit
CTAGACGTAAGCCTTCTCCTCACTCTCTCAATCTTATCCATCATAGCAGG30.16519823788546256No Hit
GTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAG30.16519823788546256No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA30.16519823788546256No Hit
GTTAAGCTACACTCTGGTTCGTCCAAGTGCACTTTCCAGTACACTTACCA30.16519823788546256No Hit
CCTCTGGGACTCAGAAGTGAAAGGGGGCTATTCCTAGTTTTATTGCTATA30.16519823788546256No Hit
GCCATAGAAGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACCTG30.16519823788546256No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC30.16519823788546256No Hit
TGTTAGTTCAAGCCGCACCGATTTGTAAGCCCCCACCATTTCACAGACTC30.16519823788546256No Hit
ACAGTACATAGTACATAAAGCCATCTACCGTACATAGCACATTACAGTCA20.11013215859030838No Hit
AGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATA20.11013215859030838No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCT20.11013215859030838No Hit
AGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAG20.11013215859030838No Hit
TGACTGTAATGTGCTATGTACGGTAGATGGCTTTATGTACCTGTCTCTTA20.11013215859030838No Hit
GAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAAC20.11013215859030838No Hit
CACGTGCACCTTGAAGATCATATTTGCGTGATAGGTACCACACCTGTCTC20.11013215859030838No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC20.11013215859030838No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT20.11013215859030838No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT20.11013215859030838No Hit
GGTAAGAAGTGGGCTAGGGCATTTTTAATCTTAGAGCGAAAGCCTATAAT20.11013215859030838No Hit
CCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAG20.11013215859030838No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT20.11013215859030838No Hit
GTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGCTGTCT20.11013215859030838No Hit
GAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCT20.11013215859030838No Hit
ATAAACCCTAGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGCT20.11013215859030838No Hit
ACATAATGGGGGTATGAGTTTTTTTTTGTTAGGGTTAACGAGGGTGGTAA20.11013215859030838No Hit
CACAGGGTCTGAGTTTTGCTCAGCACATGGCATAATCCTCAAAGCTTTCA20.11013215859030838No Hit
ATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTG20.11013215859030838No Hit
GAGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGT20.11013215859030838No Hit
GTAGTAGGGTCGTGGTGCTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTA20.11013215859030838No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA20.11013215859030838No Hit
CTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCCTGCCATC20.11013215859030838No Hit
CTATGTGGGAGAAGGAGAGAGATTTGGGAGAGGAGAAAAAAGACTTCAAT20.11013215859030838No Hit
CCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAGTTATGTCAT20.11013215859030838No Hit
GACTAGTATGGCAATAGGCACAATATTGGCTAAGAGGGAGTGGGTGTTGA20.11013215859030838No Hit
AGCAGGTAATCCATGAGGGCAACTGGGACTCAACCTGCTAACTGTCTCTT20.11013215859030838No Hit
CAGGAAAAGAGAGCTGAAATAAGGAAGAATTTGATAACTTCTTTACATGT20.11013215859030838No Hit
ACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAA20.11013215859030838No Hit
AATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTCCTGTC20.11013215859030838No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC20.11013215859030838No Hit
ATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTGCCAAAGCCTCC20.11013215859030838No Hit
GATCAGGGCGTAGTTTGAGTTTGATGCTCACCCTGATCAGAGGATTGAGT20.11013215859030838No Hit
AGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCA20.11013215859030838No Hit
GCTTTAAGCAATTTCCCATACCAGAAGCAGGGCTTAGTCACCGTCCTGTC20.11013215859030838No Hit
CCCCCACTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCCTGTCTC20.11013215859030838No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT20.11013215859030838No Hit
AATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCG20.11013215859030838No Hit
CAACAGAAACAAAGCATACATCATTATTCTCGCACGGGCTACAACCACGA20.11013215859030838No Hit
GTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGA20.11013215859030838No Hit
CTCCTAGCTTGTTAGGGACGGATCGGAGAATTGTGTAGGCGAATAGGAAA20.11013215859030838No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG20.11013215859030838No Hit
GAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGT20.11013215859030838No Hit
TTTCTGCTCTATCTCCACCTCCTTTTCCCAGCCTTCAGAAGGCAGCTGCA20.11013215859030838No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC20.11013215859030838No Hit
AGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATA20.11013215859030838No Hit
CACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCCTGTC20.11013215859030838No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAA20.11013215859030838No Hit
ACACTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGCCAGA20.11013215859030838No Hit
ATATCTAACAACGTAAAAATAAAATGACAGTTTGAACATACAAAACCCAC20.11013215859030838No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTC20.11013215859030838No Hit
CTGCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACT20.11013215859030838No Hit
CACTACGCCGGTGATTGTTGAGGGCGTTGTCTACGCATTAGGTTACAACT20.11013215859030838No Hit
GGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGCCGATACGGTTG20.11013215859030838No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA20.11013215859030838No Hit
GTCGAAGAATTAGTGAAGATTTGACAGAGTTACCAGATAAGATGTCATTT20.11013215859030838No Hit
GTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTA20.11013215859030838No Hit
CTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCTGTCTCT20.11013215859030838No Hit
TACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTT20.11013215859030838No Hit
ATGTGAAAGGGGCGTCTGAAAGGTACAGCCAATGAAATAGCGACAAGAAT20.11013215859030838No Hit
ATCTCCGGCAATCAGATCTGCTCTCTGGTGGAAATGGCCTTTGGAAAGCG20.11013215859030838No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC20.11013215859030838No Hit
CCCTATCTAGGCCTTCTCGCGAGCCAAAACCTGCCCCTACTCCTCCTAGA20.11013215859030838No Hit
ACCTTTTCCTCCGACCCCCTAACAACCCCCCTCCTAATACTAACTACCTG20.11013215859030838No Hit
CGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGGATT20.11013215859030838No Hit
GGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCCTGTCT20.11013215859030838No Hit
TAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCAC20.11013215859030838No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA20.11013215859030838No Hit
CCTTAGCATGATTTATCCTACACTCCAACTCATGAGACCCACAACAAATA20.11013215859030838No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA20.11013215859030838No Hit
AGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGGGCACCGATTATT20.11013215859030838No Hit
GTTTGAGTTTGATGCTCACCCTGATCAGAGGATTGAGTAAACGGCTAGGC20.11013215859030838No Hit
CCCAACAATGACTAATCAAACTAACCTCAAAACAAATAATAACCATACAC20.11013215859030838No Hit
CAACCTCCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC20.11013215859030838No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACC20.11013215859030838No Hit
ATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTT20.11013215859030838No Hit
GTGTACGTGGCGTTTCGTCACTGGTCTCATTCTGTTCTCCATCCCCAACT20.11013215859030838No Hit
CGCCTGTAATCTCAGCCACTAGGGAGGCTGAGGCATTTTTTATGTTTTGT20.11013215859030838No Hit
CAACTTACTATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTC20.11013215859030838No Hit
CGATAAACCCTAGGAAGCCAATTGATATCATAGCTCAGACCATACCTATG20.11013215859030838No Hit
ACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTAC20.11013215859030838No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA20.11013215859030838No Hit
TGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGG20.11013215859030838No Hit
ATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTG20.11013215859030838No Hit
CTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGC20.11013215859030838No Hit
GATGCACACGAAGTCTGAAAGGACAGCCAGGAAATTCTGAGCAAGCCCGC20.11013215859030838No Hit
ATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCAAACAAC20.11013215859030838No Hit
GACAGGAACCTTTCTCTTCCTCCTCCCTAACCCACTGCTGACATCACAGA20.11013215859030838No Hit
ACATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGCTGT20.11013215859030838No Hit
GGCTCACATCTGTAATCCTAGCACTTTGGGAGGCCCAGGCAGGTGGAACC20.11013215859030838No Hit
GGAAATATCATTCGGGCTTGATGTGGGGAGGGGTGTTTAAGGGGTTGGCT20.11013215859030838No Hit
CCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAATTACCTG20.11013215859030838No Hit
CAACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCA20.11013215859030838No Hit
ACCGTACATAGCACATTACAGTCAAATCCCTTCTCGTCCCCATGGATGAC20.11013215859030838No Hit
GTCCCAACTACTGGACTGCATATTACATCACTAGGGGGAAAGGGGAAGAC20.11013215859030838No Hit
GGTAAATGCGACCTGCATCAACGATAAAGTCGTTCACCGAGCCCAGTTCA20.11013215859030838No Hit
CAGGTGTGCATCCCACGACCAGGCCTCCCTCTCACAGAACCTGTCTCTTA20.11013215859030838No Hit
CACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTA20.11013215859030838No Hit
CCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTAAAAC20.11013215859030838No Hit
CCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAATGTTCAAC20.11013215859030838No Hit
ATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCG20.11013215859030838No Hit
CCATAATTTACGTCTCGAGTGATGTGGGCGATTGATGAAAAGGCGGTTGA20.11013215859030838No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC20.11013215859030838No Hit
AAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCCTGTCT20.11013215859030838No Hit
TAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGCT20.11013215859030838No Hit
TAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTC20.11013215859030838No Hit
GCTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTGGGTGC20.11013215859030838No Hit
CTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCACCCTCGTTAACCC20.11013215859030838No Hit
GAGTGGTGATAGCGCCTAAGCATAGTGTTAGAGTTTGGATTAGTGCTGTC20.11013215859030838No Hit
TCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTT20.11013215859030838No Hit
GGTGGTCAAGGGACCCCTATCTGAGGGGGGTCATCCATGGGGACGAGAAG20.11013215859030838No Hit
ATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATTTT20.11013215859030838No Hit
GGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAAT20.11013215859030838No Hit
GTTTATTTCTAGGCCTACTCAGGTAAAAAATCAGTGCGAGCTTAGCGCTG20.11013215859030838No Hit
GTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGA20.11013215859030838No Hit
GTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGC20.11013215859030838No Hit
CTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCCTGTCTCT20.11013215859030838No Hit
GGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGTAGCTGT20.11013215859030838No Hit
GATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCC20.11013215859030838No Hit
CTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTTTTGATGACTTCTA20.11013215859030838No Hit
CAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATTAATCATCATC20.11013215859030838No Hit
GATGAAGTGGATCGCGCGGTGAATTTTGGTGTCGACATCATCGGCATCAA20.11013215859030838No Hit
GAAGCAGAGGCTCATCAGGAAAATGAGCTCCCAGAGCCCCACCCAGGGGC20.11013215859030838No Hit
GTTTTTCATGAGGACTCTCTATAGTTCTAAGAACAGAAAGCTGCCAGGTG20.11013215859030838No Hit
AAGGGACCCCTATCTGAGGGGGGTCATCCATGGGGACGAGAAGGGATTTG20.11013215859030838No Hit
CGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGC20.11013215859030838No Hit
GACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTC20.11013215859030838No Hit
GTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTC20.11013215859030838No Hit
ACATGGGGGCATGAGTTAGCAGTTCTTGTGAGCTTTCTCGGTAAATAAGG20.11013215859030838No Hit
ACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAAT20.11013215859030838No Hit
AATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGC20.11013215859030838No Hit
CCCTACTAAACCCCATTAAACGCCTGGCAGCCGGAAGCCTATTCGCAGGA20.11013215859030838No Hit
ATTGGCAGGAGCCATAGATACAAACCCAACCACTTGCTCCAAAAAGTGCT20.11013215859030838No Hit
GACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACA20.11013215859030838No Hit
CTGCTAGTGTGGAGATAAATCATATTATGGCCAAGGGTCATGATGGCAGG20.11013215859030838No Hit
GGTTAAAGGAGCCACTTATTAGTAATGTTGATAGTAGAATGATGGCTAGG20.11013215859030838No Hit
CAGGTTGTAGCCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAG20.11013215859030838No Hit
CAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATA20.11013215859030838No Hit
TTTCAGCAGGCAGAATTCTGACATCTGCCTGGGTTCTCCTGGGGCTGCCA20.11013215859030838No Hit
ACTATTAACAAAATATCCCCTAGAAAGCCTGGACCACTTCACACACCCTG20.11013215859030838No Hit
ATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAG20.11013215859030838No Hit
CAACAATCCCCCTCTACCTAAAACTCACAGCCCTCGCTGTCACTTTCCTA20.11013215859030838No Hit
TCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGA20.11013215859030838No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT20.11013215859030838No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC20.11013215859030838No Hit
GAGACCCTGTGGGGCGTGAGAGGAAGGGGGAGAGGGAAGAGGCTGTCTCT20.11013215859030838No Hit
ATCTTTAACAGTACATAGTACATAAAGCCATCTACCGTACATAGCACATT20.11013215859030838No Hit
GACTCGCACACTCCTCCTCCCCAGCCTTCTCCTCCTATGAGAGGCTGTCT20.11013215859030838No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)