FastQCFastQC Report
Wed 25 May 2016
SRR1780545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32040
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGCCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT590.18414481897627966No Hit
CCTTAGCATGATTTATCCTACACTCCAACTCATGAGACCCACAACAAATA330.10299625468164794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAC207.619969E-444.1415137
TCGCTCA207.859674E-443.8656279
GATTCAG250.002282377635.3132119
GTCCAAT250.002335853435.147421
TAAAATC250.002353897335.0925034
CTGCACC250.002353897335.0925034
TGTTAGC300.005574194729.42767120
TCTCAAC300.005574194729.42767113
ATCGCTC300.00574818729.243758
CTCATGA300.00574818729.2437529
CTAGAGG550.004309113620.06432340
CTTATAC1001.0866188E-619.73953244
TCTTATA1402.671299E-514.09966643
CTCTTAT2003.0641786E-713.15968844
TCTCTTA2504.533151E-610.52774943
GTCTCTT3251.0389813E-58.77312544
CCTGTCT2050.0096561188.61297839
TGTCTCT4102.7776696E-78.55914644
CTGTCTC4651.8761275E-67.54677443
Produced by FastQC (version 0.11.5)