FastQCFastQC Report
Wed 25 May 2016
SRR1780545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32040
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT4011.2515605493133584No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT560.17478152309612985RNA PCR Primer, Index 17 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG480.149812734082397No Hit
GTTGTAGCCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT420.1310861423220974No Hit
ATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACC390.12172284644194756No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG370.11548064918851435No Hit
CTTCTAGTAAGCCTCTACCTGCACGACAACACATAATGACCCACCAATCA350.10923845193508115No Hit
GGTTAAAGGAGCCACTTATTAGTAATGTTGATAGTAGAATGATGGCTAGG350.10923845193508115No Hit
CTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATAT350.10923845193508115No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT340.10611735330836455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTTG207.744879E-443.99687228
GTTGGAT207.744879E-443.99687231
CAAAAAA207.744879E-443.99687227
GGCTGTG250.002319646335.19757
ATGTTGG250.002319646335.197529
TACCCAC250.002319646335.19755
ATGCCGT502.2693384E-630.79781244
TGTTGGA300.005664863629.3312530
TATGTGT300.005664863629.3312520
GGTAGGC300.005664863629.3312513
AAGTTGA300.005664863629.331256
TATGCCG554.768661E-627.9980143
ACAGATC508.099018E-526.39812533
CAGATCT508.099018E-526.39812534
AGATCTC508.099018E-526.39812535
GGGTAGG450.001369294224.44270912
AGCCCAC551.5476196E-423.99829517
GATCTCG551.5476196E-423.99829536
ATCTCGT651.7438899E-523.69062437
TCTCGTA651.7438899E-523.69062438