FastQCFastQC Report
Wed 25 May 2016
SRR1780544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12729
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACC200.006795570352.53
GTGGAGC200.006795570352.567
CTGCCGA700.001161032425.070
GCTGCCG800.002526058321.87569
CGCTGCC850.003591222520.58823668
ACGCTGC900.005000462819.44444367
TCTGACG1550.006860279513.54838763
CATCTGA1600.00846458813.12561
Produced by FastQC (version 0.11.5)