##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780544_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12729 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.336947128603974 34.0 33.0 34.0 23.0 34.0 2 32.60436797863147 34.0 34.0 34.0 31.0 34.0 3 32.706261293110224 34.0 34.0 34.0 31.0 34.0 4 33.07502553224919 34.0 34.0 34.0 32.0 34.0 5 33.04171576714589 34.0 34.0 34.0 32.0 34.0 6 36.22853327048472 38.0 37.0 38.0 34.0 38.0 7 35.71066069604839 38.0 37.0 38.0 31.0 38.0 8 34.64757640034567 38.0 35.0 38.0 26.0 38.0 9 35.442139995286354 38.0 36.0 38.0 31.0 38.0 10-11 35.80351952235054 38.0 37.0 38.0 32.0 38.0 12-13 35.96755440333098 38.0 37.0 38.0 32.5 38.0 14-15 35.839421792756696 38.0 37.0 38.0 31.5 38.0 16-17 35.70740042422814 38.0 37.0 38.0 31.0 38.0 18-19 35.67684028596119 38.0 37.0 38.0 31.5 38.0 20-21 35.71816324927332 38.0 37.0 38.0 31.0 38.0 22-23 35.65134731714981 38.0 36.5 38.0 31.5 38.0 24-25 36.06654096943986 38.0 37.0 38.0 33.0 38.0 26-27 36.03959462644356 38.0 37.0 38.0 33.5 38.0 28-29 36.273941393668 38.0 37.0 38.0 34.0 38.0 30-31 36.13308193887973 38.0 37.0 38.0 34.0 38.0 32-33 35.706968340011 38.0 37.0 38.0 31.5 38.0 34-35 35.8164820488648 38.0 37.0 38.0 31.5 38.0 36-37 35.88247309293739 38.0 37.0 38.0 31.5 38.0 38-39 36.26753869117763 38.0 37.0 38.0 34.0 38.0 40-41 35.86134024668081 38.0 37.0 38.0 32.0 38.0 42-43 36.145651661560215 38.0 37.0 38.0 34.0 38.0 44-45 35.180493361615206 38.0 36.0 38.0 29.0 38.0 46-47 35.94516458480635 38.0 37.0 38.0 33.0 38.0 48-49 35.73752847827795 38.0 37.0 38.0 31.5 38.0 50-51 35.58865582528085 38.0 36.5 38.0 31.5 38.0 52-53 35.52584649226176 38.0 36.0 38.0 31.0 38.0 54-55 35.37406709089481 38.0 36.0 38.0 31.0 38.0 56-57 35.374459894728574 38.0 36.0 38.0 29.0 38.0 58-59 35.34782779479927 37.5 36.0 38.0 29.0 38.0 60-61 35.38722601932595 38.0 36.0 38.0 30.5 38.0 62-63 35.3866368135753 38.0 36.0 38.0 31.0 38.0 64-65 35.50628486134025 38.0 36.0 38.0 31.0 38.0 66-67 35.445793070940375 38.0 36.0 38.0 31.0 38.0 68-69 34.856823002592506 37.5 35.5 38.0 27.5 38.0 70-71 34.99021918453924 37.0 36.0 38.0 27.0 38.0 72-73 35.0222719773745 37.0 36.0 38.0 27.5 38.0 74-75 34.60978867153743 37.0 34.5 38.0 26.0 38.0 76 33.969282740199546 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -4.040999561508304 1101 2 -0.9919286012166779 1101 3 -1.3243256626852045 1101 4 -1.3976487106277347 1101 5 -2.265013605572342 1101 6 -2.8509784636556006 1101 7 -1.0914637604009698 1101 8 -1.376191567444927 1101 9 -0.8031203196483645 1101 10-11 -1.7786841849832697 1101 12-13 -1.534378817938908 1101 14-15 -0.6386933540206883 1101 16-17 -2.084663334155074 1101 18-19 -0.02382944394170039 1101 20-21 -1.730483938805122 1101 22-23 -1.2394135673291586 1101 24-25 -2.1649796718248453 1101 26-27 -2.1976063801149195 1101 28-29 -1.6680721604350879 1101 30-31 -2.6748671552072736 1101 32-33 -0.5041373247873224 1101 34-35 -0.8129269872223972 1101 36-37 -1.0184956742820788 1101 38-39 -1.5154669426041991 1101 40-41 -0.2118756129713475 1101 42-43 -1.3922521696433208 1101 44-45 0.29121289983523013 1101 46-47 -0.3451138005681571 1101 48-49 -0.729694402930896 1101 50-51 -1.097621658831855 1101 52-53 -1.813014978409683 1101 54-55 -1.8092044772037212 1101 56-57 -1.625642252012426 1101 58-59 -0.06978764144522387 1101 60-61 -0.28601299514264866 1101 62-63 -1.970033085040832 1101 64-65 -0.6710230977767822 1101 66-67 -1.7411110035807198 1101 68-69 -0.48956672827108605 1101 70-71 -0.26592250448999977 1101 72-73 -1.1392074338934393 1101 74-75 -3.1885998723007347 1101 76 -2.5314014037232297 1102 1 0.09057938586011716 1102 2 -0.032864273731298965 1102 3 0.49438778760719515 1102 4 0.1959185408342563 1102 5 -0.23577383949047004 1102 6 0.494050776110484 1102 7 0.6161385787803155 1102 8 2.0360891343094636 1102 9 0.7246574581294141 1102 10-11 0.4866666922097167 1102 12-13 -5.702507472909666 1102 14-15 -2.306822008991446 1102 16-17 -2.4092247376638483 1102 18-19 -0.7051159936493008 1102 20-21 0.4032879910194467 1102 22-23 -0.3183609357502135 1102 24-25 0.09744722875995393 1102 26-27 -5.968805210524273 1102 28-29 -0.16661017213099427 1102 30-31 0.5685538974243016 1102 32-33 -7.686885862799016 1102 34-35 -0.8721375135381919 1102 36-37 0.8857440917997934 1102 38-39 0.6767845193841069 1102 40-41 -0.5415539755444385 1102 42-43 0.025876485327437138 1102 44-45 -6.982178913030264 1102 46-47 -0.03151730934008157 1102 48-49 0.14530559706910395 1102 50-51 -8.421452068188579 1102 52-53 -0.7391845690529593 1102 54-55 0.8340703765974453 1102 56-57 -3.1607299713106727 1102 58-59 0.2562357503676438 1102 60-61 -0.07475568520113285 1102 62-63 -5.331144196151946 1102 64-65 -0.9911985363732683 1102 66-67 0.02716385022044676 1102 68-69 -9.212519944645358 1102 70-71 -1.1358055454256757 1102 72-73 -0.7400846268759054 1102 74-75 -8.71491566177442 1102 76 -4.344266900799251 1103 1 -2.1714343441169994 1103 2 -1.7914455094292379 1103 3 -1.0731179332166043 1103 4 -1.0111752806760421 1103 5 -0.38337109349504317 1103 6 -3.838901168969606 1103 7 -1.147019315956527 1103 8 -0.7167712775898494 1103 9 -0.42751645491406265 1103 10-11 -0.06310447483834025 1103 12-13 -2.3809971754268275 1103 14-15 -2.159224754986873 1103 16-17 -2.5121995660391363 1103 18-19 -1.5769695405600572 1103 20-21 -2.2322955330080134 1103 22-23 -1.945935306459596 1103 24-25 -0.4506076911485124 1103 26-27 -2.5460363318057304 1103 28-29 -0.19464220874426275 1103 30-31 -1.1150845465116177 1103 32-33 -5.4546204165747625 1103 34-35 -1.7205356828745693 1103 36-37 -1.000983596987389 1103 38-39 -0.36268916482642055 1103 40-41 0.1764094111832506 1103 42-43 -0.24128598606844065 1103 44-45 -6.729318501130951 1103 46-47 -0.09330220636525866 1103 48-49 -0.4742596203222007 1103 50-51 -0.9569211757400709 1103 52-53 -1.7037154615014671 1103 54-55 -0.5326344288945464 1103 56-57 -0.11839587520083228 1103 58-59 -1.1362127622181717 1103 60-61 0.195267198094065 1103 62-63 -1.483318109195423 1103 64-65 0.2999913949768427 1103 66-67 0.008285131684978353 1103 68-69 -3.845243056773505 1103 70-71 -0.3154394127025597 1103 72-73 0.36803894291815453 1103 74-75 -3.0472955244746487 1103 76 -1.3454110655589773 1104 1 -0.6634485411001414 1104 2 0.6384568863116655 1104 3 0.8842911173601493 1104 4 0.7984964140888167 1104 5 0.2599297050852556 1104 6 0.684168928634648 1104 7 1.9244092554720424 1104 8 1.0591825822149374 1104 9 0.7960860295579835 1104 10-11 0.7170641143364591 1104 12-13 0.29668694169828314 1104 14-15 0.25869893622874685 1104 16-17 -0.021738164087047096 1104 18-19 0.955195499368962 1104 20-21 1.4540398707186881 1104 22-23 1.1942599020585973 1104 24-25 1.3007232760209604 1104 26-27 1.4812484951685292 1104 28-29 -0.13519234184098394 1104 30-31 0.026663457037621185 1104 32-33 -0.42533913884627594 1104 34-35 0.07737913522657891 1104 36-37 0.43246804453877985 1104 38-39 0.6066985902756201 1104 40-41 0.9119906001805802 1104 42-43 1.1083147237807154 1104 44-45 -1.9950682793030907 1104 46-47 0.7203623899080398 1104 48-49 1.4718362093139987 1104 50-51 1.4740903593087324 1104 52-53 -0.20615556797884693 1104 54-55 0.4844463164470696 1104 56-57 1.2638135303004958 1104 58-59 1.425677211162487 1104 60-61 1.3095085468074572 1104 62-63 1.5821211099705081 1104 64-65 0.572174181134784 1104 66-67 0.1316214227231356 1104 68-69 -2.904249267953631 1104 70-71 -0.03349620063512759 1104 72-73 1.0869830422970281 1104 74-75 -0.23451823964767726 1104 76 1.1159908865262054 1105 1 0.25445498394624266 1105 2 0.7302936210055435 1105 3 0.5721389837794462 1105 4 0.766492703513677 1105 5 0.7905419499832149 1105 6 0.79548618280905 1105 7 1.9438897749525665 1105 8 1.229869038615682 1105 9 0.47465745812941407 1105 10-11 0.9448006635015815 1105 12-13 -1.4788232623833508 1105 14-15 0.6327712924439624 1105 16-17 0.9191245446328011 1105 18-19 0.08854429343203662 1105 20-21 -0.7229081812293572 1105 22-23 -0.3757772036927989 1105 24-25 -0.553868560713731 1105 26-27 -0.35038415789269095 1105 28-29 -1.1971125644754963 1105 30-31 0.27967829933817967 1105 32-33 1.330458634808636 1105 34-35 1.2893457400503365 1105 36-37 0.6052416994552914 1105 38-39 0.8418562897190327 1105 40-41 -0.3734917745874995 1105 42-43 0.9953740929829351 1105 44-45 1.0967684553907873 1105 46-47 0.31776498731063185 1105 48-49 0.6793965061600105 1105 50-51 0.5336914724812729 1105 52-53 -0.9897826551773576 1105 54-55 -0.4051640731633128 1105 56-57 -0.4854907368609105 1105 58-59 -0.182161378818968 1105 60-61 -0.044851378981036305 1105 62-63 0.9643103493026004 1105 64-65 -0.12935643111011075 1105 66-67 0.45838394591422826 1105 68-69 1.422049433345066 1105 70-71 1.6153906268231282 1105 72-73 0.9782168085307958 1105 74-75 -0.04087259957346845 1105 76 0.47869960637778064 1106 1 -0.9068532200448907 1106 2 0.32785459661529615 1106 3 -1.134623765666234 1106 4 -0.5101148352889808 1106 5 0.13200536461736334 1106 6 -0.29542290810004346 1106 7 0.8651758059946957 1106 8 0.9815320097908469 1106 9 0.6636818483733151 1106 10-11 0.012982481683401659 1106 12-13 0.08215234737274812 1106 14-15 -0.46728413992854456 1106 16-17 0.596230974788007 1106 18-19 0.7329456238089804 1106 20-21 -0.2010123940896733 1106 22-23 -0.14795015269501022 1106 24-25 0.2113753417252937 1106 26-27 0.6191280372292596 1106 28-29 -0.902489504608532 1106 30-31 0.20013284479272642 1106 32-33 0.6577867944538696 1106 34-35 0.5071949639971152 1106 36-37 0.3876696373710402 1106 38-39 0.47877424980772787 1106 40-41 -0.8172279164943816 1106 42-43 0.6192654455328253 1106 44-45 0.6577440651468862 1106 46-47 -0.12458534528359877 1106 48-49 0.7672568165813018 1106 50-51 0.7739908072928117 1106 52-53 -0.44682256648555807 1106 54-55 0.6935055498965497 1106 56-57 0.9820813252233407 1106 58-59 0.05758363226751584 1106 60-61 0.30104662545355865 1106 62-63 1.1383679989700042 1106 64-65 0.6968630810850129 1106 66-67 0.6365990235195511 1106 68-69 0.6706636240324357 1106 70-71 -0.17784662373119176 1106 72-73 -0.2388009298284075 1106 74-75 -1.2800632869348831 1106 76 0.2314712250030624 1107 1 -0.09655511706386122 1107 2 0.2525158432277692 1107 3 0.8534521150925727 1107 4 0.2356846227055982 1107 5 0.7016530610943263 1107 6 0.7712437585666265 1107 7 0.7974251284879159 1107 8 1.6682528769995173 1107 9 0.15799079146274408 1107 10-11 -0.19812862942770693 1107 12-13 0.5100656265055434 1107 14-15 -0.1331377984651283 1107 16-17 0.3097811102893715 1107 18-19 1.1928372227249682 1107 20-21 0.6111827278615465 1107 22-23 0.5883642104486242 1107 24-25 0.443353661508489 1107 26-27 0.777393619885089 1107 28-29 -0.15696104932397503 1107 30-31 0.20013284479272642 1107 32-33 0.9347515641015676 1107 34-35 0.5676285683331628 1107 36-37 0.4676154368290355 1107 38-39 1.0928663907291352 1107 40-41 0.7686799425842139 1107 42-43 -0.0311410585322065 1107 44-45 0.3967684553907844 1107 46-47 0.27433064387629 1107 48-49 0.8230833748468811 1107 50-51 0.7496005633903664 1107 52-53 1.0453183549236513 1107 54-55 0.6574621894629402 1107 56-57 1.113246636876454 1107 58-59 1.0191012474436647 1107 60-61 0.6167647826351228 1107 62-63 0.9521891371813851 1107 64-65 0.9234213466676664 1107 66-67 0.7894445519748388 1107 68-69 1.5909888272844626 1107 70-71 0.6785219399544431 1107 72-73 1.1441258994398922 1107 74-75 0.43917790547704527 1107 76 -0.38695695927878404 1108 1 -0.10918137969012065 1108 2 -0.2697063789944565 1108 3 -0.4733155616751006 1108 4 0.3801290671500439 1108 5 0.5632692227104883 1108 6 -0.22724108991822334 1108 7 0.852980684043473 1108 8 1.479869038615682 1108 9 0.1110210944930472 1108 10-11 0.8311642998652218 1108 12-13 0.6348131012530089 1108 14-15 1.3941349288076026 1108 16-17 -0.6604209099126521 1108 18-19 -1.1955466156588699 1108 20-21 0.049819091497909085 1108 22-23 -0.5235044764200651 1108 24-25 -0.8379594698046375 1108 26-27 0.02461584210730905 1108 28-29 0.16652379916087057 1108 30-31 0.42740557206545304 1108 32-33 0.03500408935409638 1108 34-35 1.1757093764139626 1108 36-37 0.7302416994552914 1108 38-39 0.9554926533553925 1108 40-41 1.3765082254124934 1108 42-43 1.120374092982935 1108 44-45 1.1535866372089743 1108 46-47 0.8518558964015384 1108 48-49 0.6339419607054637 1108 50-51 -0.3185812547914466 1108 52-53 -0.023873564268264147 1108 54-55 -0.13243680043604655 1108 56-57 1.173600172229996 1108 58-59 -2.0457977424553278 1108 60-61 0.22787589374623707 1108 62-63 0.16885580384805365 1108 64-65 0.541098114344436 1108 66-67 -0.3370705995403185 1108 68-69 1.7743221606177997 1108 70-71 1.785845172277675 1108 72-73 1.671398626712616 1108 74-75 2.0273092186083588 1108 76 0.8877905154686943 1109 1 1.134000438491693 1109 2 0.18029362100554636 1109 3 -0.34377010712965017 1109 4 0.005129067150043909 1109 5 0.16554194998321492 1109 6 0.32957709189995654 1109 7 -1.4720193159565298 1109 8 0.18214176588841013 1109 9 0.47465745812941407 1109 10-11 0.6629824816834002 1109 12-13 -0.6913232623833494 1109 14-15 0.6793622015348717 1109 16-17 -0.3763300008217456 1109 18-19 0.6442261116138539 1109 20-21 0.5486827278615465 1109 22-23 0.44808643267084136 1109 24-25 0.28363143928627466 1109 26-27 0.36211584210730763 1109 28-29 0.4222056173426907 1109 30-31 0.18763284479272357 1109 32-33 1.1111404529904618 1109 34-35 -0.5504269872223944 1109 36-37 0.6995598812734798 1109 38-39 0.474810835173578 1109 40-41 0.08673549813976678 1109 42-43 -0.022807725198880746 1109 44-45 -1.1157315446092113 1109 46-47 -0.6992804672348214 1109 48-49 0.4703055970691068 1109 50-51 1.524600563390372 1109 52-53 0.689762799368097 1109 54-55 1.1005177450185002 1109 56-57 0.5451910813209011 1109 58-59 1.2871568029992133 1109 60-61 0.9653758937462342 1109 62-63 -0.05614419615194066 1109 64-65 -0.09185643111010933 1109 66-67 0.2811112186414988 1109 68-69 1.0368221606178025 1109 70-71 0.11879971773222309 1109 72-73 0.8316258994398922 1109 74-75 1.7489001276992653 1109 76 -8.458481676711926E-4 1110 1 -1.2845893050980486 1110 2 -0.6158602251483032 1110 3 -1.6405649789245196 1110 4 -0.1967940097730363 1110 5 0.4059265653678281 1110 6 -0.24414085681799236 1110 7 0.9940063250691153 1110 8 0.9571417658884087 1110 9 -0.18559895212699473 1110 10-11 -0.32035085164992694 1110 12-13 0.05963827607818217 1110 14-15 0.8591698938425623 1110 16-17 0.767900768409028 1110 18-19 0.14070047058822155 1110 20-21 -0.6503557336769106 1110 22-23 0.7669966890810969 1110 24-25 0.19292631108114477 1110 26-27 -0.5491021066106399 1110 28-29 -0.031320023682951614 1110 30-31 0.4437225883824638 1110 32-33 0.9338968632468649 1110 34-35 -0.5084398077352148 1110 36-37 0.027444496658091566 1110 38-39 0.11166980953255745 1110 40-41 0.9473124212166866 1110 42-43 0.03296150557034849 1110 44-45 0.8659992246215609 1110 46-47 -0.0598573903117412 1110 48-49 0.5555620073255128 1110 50-51 -0.5709122571224512 1110 52-53 0.2051474147527088 1110 54-55 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0.7589419607054637 2116 50-51 1.34050965429946 2116 52-53 1.8397627993680956 2116 54-55 0.9357450177457736 2116 56-57 1.9349638085936363 2116 58-59 1.056474984817406 2116 60-61 -0.6584877426174032 2116 62-63 1.5097648947571471 2116 64-65 1.4956435688898893 2116 66-67 1.0379294004596815 2116 68-69 2.2515948878905263 2116 70-71 -0.6346093731768718 2116 72-73 1.9441258994398893 2116 74-75 0.9932183095174452 2116 76 -0.29402766634949273 2117 1 0.10605926202110894 2117 2 -0.12264755546504347 2117 3 1.1135828340468237 2117 4 -0.5904591681440721 2117 5 -0.9741639323697271 2117 6 -1.5895405551588695 2117 7 -0.23525461007417192 2117 8 -2.3957994105821783 2117 9 0.8717162816588271 2117 10-11 -0.3105469300813084 2117 12-13 1.5505885023225332 2117 14-15 1.1080386721231008 2117 16-17 1.2045523521194283 2117 18-19 1.0464319939667988 2117 20-21 0.3700062572733174 2117 22-23 0.5694099620826094 2117 24-25 0.2623079098745009 2117 26-27 0.5466746656367221 2117 28-29 1.2861762055779806 2117 30-31 0.9942504918515525 2117 32-33 1.2589345706375141 2117 34-35 0.9591318363070158 2117 36-37 0.6179422342146523 2117 38-39 1.1961343645853475 2117 40-41 -0.9463527371543492 2117 42-43 -1.1095724310812258 2117 44-45 0.8908861024496133 2117 46-47 0.30439600335341055 2117 48-49 1.2923644205985099 2117 50-51 1.3966593869197723 2117 52-53 1.2662333876033856 2117 54-55 0.6777236273714422 2117 56-57 0.1635734342620765 2117 58-59 0.08053915594039296 2117 60-61 1.610228834922708 2117 62-63 -0.7576147843872363 2117 64-65 -0.2837681958159948 2117 66-67 -0.49682995782909245 2117 68-69 0.2890280429707417 2117 70-71 0.6621820706733956 2117 72-73 1.4147141347340053 2117 74-75 0.03198836299338126 2117 76 -0.7111399658147306 2118 1 0.9590004384916959 2118 2 1.0636269543388792 2118 3 1.1201187817592384 2118 4 0.7134624004833796 2118 5 0.012764172205436353 2118 6 1.2045770918999565 2118 7 0.5752029062656945 2118 8 -0.04285823411159129 2118 9 0.05799079146274977 2118 10-11 -0.3481286294277126 2118 12-13 0.9933989598388777 2118 14-15 -1.4720266873540169 2118 16-17 0.332003332511583 2118 18-19 -0.5793849994972575 2118 20-21 -2.299928383249565 2118 22-23 0.12169754378195563 2118 24-25 -0.5399796718248453 2118 26-27 1.2607269532184233 2118 28-29 0.13748339512046925 2118 30-31 0.14457728923716928 2118 32-33 -1.0874706581206581 2118 34-35 0.4787396794442742 2118 36-37 -0.2823845631709645 2118 38-39 -0.5293558314930848 2118 40-41 -1.1424311685269046 2118 42-43 -0.08669661408776363 2118 44-45 1.0967684553907873 2118 46-47 0.8354417549874 2118 48-49 -1.3824721807086675 2118 50-51 -0.6115105477207479 2118 52-53 -0.24357053396523298 2118 54-55 0.44079552279627876 2118 56-57 0.8188021924320168 2118 58-59 1.0413234696658833 2118 60-61 -0.41101299514264866 2118 62-63 1.2521891371813823 2118 64-65 0.5234213466676678 2118 66-67 -2.171666559136277 2118 68-69 1.3020999383955782 2118 70-71 0.15074416217666453 2118 72-73 -1.8614296561156678 2118 74-75 1.3669556832548224 2118 76 2.0797097073878845 2119 1 -0.6179226384313807 2119 2 0.6918320825440034 2119 3 1.1543068159472725 2119 4 0.03397522099619721 2119 5 0.7136188730601347 2119 6 1.6276540149768763 2119 7 -0.49317316211037365 2119 8 1.4956033043499488 2119 9 1.0708113042832608 2119 10-11 1.0129824816834017 2119 12-13 1.5596382760781893 2119 14-15 0.7437852784579491 2119 16-17 -0.8090223085140522 2119 18-19 -0.06442773453998996 2119 20-21 -0.07343265675383748 2119 22-23 1.5875095095939216 2119 24-25 1.1288237469785756 2119 26-27 1.6303850728765354 2119 28-29 1.1097056173426907 2119 30-31 1.3924405371004198 2119 32-33 1.908255837605843 2119 34-35 1.0813037820083764 2119 36-37 0.7966752658888652 2119 38-39 0.8680800659428058 2119 40-41 -0.12961065570638652 2119 42-43 0.2252691978780419 2119 44-45 0.7890761476984807 2119 46-47 1.1709118404574852 2119 48-49 1.5299209816844908 2119 50-51 2.095754409544206 2119 52-53 0.282070491675789 2119 54-55 -1.202366870366113 2119 56-57 1.2653833890132091 2119 58-59 0.3361952645376789 2119 60-61 0.15095281682315687 2119 62-63 -0.27345188845963975 2119 64-65 1.091797415043736 2119 66-67 1.4330342955645818 2119 68-69 0.024322160617799682 2119 70-71 1.2255304869629953 2119 72-73 -0.7481817928678041 2119 74-75 1.2152462815454186 2119 76 -0.2450766173984391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 4.0 15 4.0 16 7.0 17 5.0 18 17.0 19 16.0 20 14.0 21 15.0 22 37.0 23 47.0 24 56.0 25 86.0 26 87.0 27 122.0 28 122.0 29 200.0 30 227.0 31 309.0 32 435.0 33 601.0 34 912.0 35 1613.0 36 3126.0 37 4666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.622162461441114 22.842679743731708 10.994226053942894 27.540931740884282 2 18.116112813261058 19.883730065205437 37.30065205436405 24.699505067169454 3 17.181239688899364 24.888050907376854 30.293031659989 27.637677743734777 4 12.931102207557545 17.283368685678372 39.67318721030717 30.112341896456908 5 12.231911383455103 37.0649697541048 37.489197894571454 13.213920967868647 6 28.05404980752612 39.68889936365779 17.75473328619687 14.502317542619217 7 26.99347945635949 30.85081310393589 23.858904862911462 18.29680257679315 8 24.275276926702805 35.100950585277715 19.907298295231364 20.716474192788123 9 23.82748055621023 14.078089402152566 22.30340168120041 39.7910283604368 10-11 25.41833608296017 25.316207086181166 28.376148951213764 20.889307879644907 12-13 24.436326498546627 23.238274805562103 27.398067405137873 24.9273312907534 14-15 21.718123968889934 25.65401838321942 24.26349281168984 28.3643648362008 16-17 23.092937387068897 27.476628171890958 26.4867625108021 22.94367193023804 18-19 21.722052007227592 27.42556367350145 27.779087123890328 23.073297195380626 20-21 22.440883023018305 27.476628171890958 26.581035430905807 23.501453374184933 22-23 22.095215649304738 27.37449917511195 27.578757168669966 22.951528006913346 24-25 22.409458716317072 27.586613245345276 27.73195066383848 22.271977374499173 26-27 21.678843585513395 27.272370178332938 27.653389897085397 23.39539633906827 28-29 22.350538141252258 27.527692670280462 28.26616387775945 21.855605310707833 30-31 22.468379291381886 27.1388168748527 28.152250765967473 22.24055306779794 32-33 20.708618116112813 28.545054599732893 28.19153114934402 22.554796134810275 34-35 22.656925131589283 27.134888836515042 27.83407966061749 22.37410637127818 36-37 22.668709246602248 26.636027967632963 27.95584884908477 22.739413936680023 38-39 22.07950349595412 27.924424542383537 28.07761803755205 21.9184539241103 40-41 21.94595019247388 26.75386911776259 28.16796291931809 23.13221777044544 42-43 21.94987823081153 26.87171026789221 28.20331526435698 22.975096236939272 44-45 21.34496032681279 26.98955141802184 28.43506952627858 23.230418728886796 46-47 21.494225783643646 26.467122319113834 29.02034723858905 23.01830465865347 48-49 20.618273234346766 26.769581271113207 29.224605232147066 23.38754026239296 50-51 21.113206064891195 26.306072747270015 28.77680886165449 23.803912326184303 52-53 20.641841464372693 26.463194280776182 29.2128211171341 23.682143137717024 54-55 19.942650640270248 26.60460366093173 29.609552989237176 23.843192709560846 56-57 20.084060020425802 26.86778222955456 29.2560295388483 23.79212821117134 58-59 20.249037630607276 26.714588734386048 28.906434126797077 24.1299395082096 60-61 20.044779637049256 26.710660696048393 28.4586377563045 24.785921910597846 62-63 19.365229004635083 26.55746720087988 29.389582842328544 24.687720952156493 64-65 20.268677822295544 26.471050357451485 28.493990101343392 24.76628171890958 66-67 20.040851598711605 26.4789064341268 28.419357372927962 25.06088459423364 68-69 20.107628250451725 26.200015712153352 27.99905727079896 25.693298766595962 70-71 20.814675151229476 26.54961112420457 27.586613245345276 25.049100479220677 72-73 21.545290282033154 25.799355801712625 27.166313143216275 25.489040773037946 74-75 20.9757247230733 26.329640977295938 27.05632806976196 25.638306229868803 76 21.76133239060413 25.288710817817584 27.1898813732422 25.760075418336083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.5 20 4.5 21 6.0 22 7.0 23 15.0 24 25.5 25 29.0 26 40.0 27 56.5 28 71.5 29 81.0 30 92.0 31 113.5 32 169.0 33 214.0 34 239.5 35 310.5 36 374.0 37 392.0 38 433.0 39 536.5 40 701.5 41 801.0 42 798.0 43 821.5 44 883.5 45 951.0 46 980.0 47 940.0 48 921.5 49 875.0 50 807.0 51 751.0 52 597.0 53 491.0 54 483.0 55 463.0 56 356.5 57 245.5 58 221.0 59 175.5 60 111.0 61 71.0 62 50.0 63 39.0 64 20.0 65 9.5 66 4.5 67 2.0 68 1.5 69 2.0 70 2.0 71 1.0 72 0.5 73 0.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6756225940765183 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12729.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.5440333097651 #Duplication Level Percentage of deduplicated Percentage of total 1 79.89808652246256 60.35823709639406 2 13.311148086522461 20.11155628878938 3 3.743760399334443 8.48456280933302 4 1.7678868552412648 5.342132139209679 5 0.5927620632279534 2.23898185246288 6 0.4263727121464226 1.9325948621258544 7 0.13519134775374378 0.71490297745306 8 0.051996672212978365 0.31424306701233407 9 0.06239600665557404 0.4242281404666509 >10 0.010399334442595673 0.07856076675308352 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.007856076675308351 0.0 17 0.0 0.0 0.0 0.007856076675308351 0.0 18 0.0 0.0 0.0 0.007856076675308351 0.0 19 0.0 0.0 0.0 0.007856076675308351 0.0 20 0.0 0.0 0.0 0.007856076675308351 0.0 21 0.0 0.0 0.0 0.007856076675308351 0.0 22 0.0 0.0 0.0 0.007856076675308351 0.0 23 0.0 0.0 0.0 0.007856076675308351 0.0 24 0.0 0.0 0.0 0.007856076675308351 0.0 25 0.0 0.0 0.0 0.007856076675308351 0.0 26 0.0 0.0 0.0 0.007856076675308351 0.0 27 0.0 0.0 0.0 0.015712153350616702 0.0 28 0.0 0.0 0.0 0.015712153350616702 0.0 29 0.0 0.0 0.0 0.05499253672715846 0.0 30 0.0 0.0 0.0 0.05499253672715846 0.0 31 0.0 0.0 0.0 0.07856076675308352 0.0 32 0.0 0.0 0.0 0.09427292010370021 0.0 33 0.0 0.0 0.0 0.16497761018147536 0.0 34 0.0 0.0 0.0 0.21211407023332549 0.0 35 0.0 0.0 0.0 0.28281876031110065 0.0 36 0.0 0.0 0.0 0.48707675386911775 0.0 37 0.0 0.0 0.0 0.7777515908555267 0.0 38 0.0 0.0 0.0 1.1548432712703276 0.0 39 0.0 0.0 0.0 1.5240788750098202 0.0 40 0.0 0.0 0.0 1.9325948621258544 0.0 41 0.0 0.0 0.0 2.506088459423364 0.0 42 0.0 0.0 0.0 3.0167334433184068 0.0 43 0.0 0.0 0.0 3.495954120512216 0.0 44 0.0 0.0 0.0 4.045879487783801 0.0 45 0.0 0.0 0.0 4.587948778380077 0.0 46 0.0 0.0 0.0 5.216434912404745 0.0 47 0.0 0.0 0.0 6.057035116662739 0.0 48 0.0 0.0 0.0 6.874067090894807 0.0 49 0.0 0.0 0.0 7.565401838321942 0.0 50 0.0 0.0 0.0 8.34315342917747 0.0 51 0.0 0.0 0.0 9.168041480084845 0.0 52 0.0 0.0 0.0 9.843664074161364 0.0 53 0.0 0.0 0.0 10.629271741692198 0.0 54 0.0 0.0 0.0 11.359886872495876 0.0 55 0.0 0.0 0.0 12.059077696598319 0.0 56 0.0 0.0 0.0 12.632571293895829 0.0 57 0.0 0.0 0.0 13.512451881530364 0.0 58 0.0 0.0 0.0 14.14879409223034 0.0 59 0.0 0.0 0.0 14.942257836436484 0.0 60 0.0 0.0 0.0 15.853562730772252 0.0 61 0.0 0.0 0.0 16.54489747819939 0.0 62 0.0 0.0 0.0 17.29908083902899 0.0 63 0.0 0.0 0.0 17.943279126404274 0.0 64 0.0 0.0 0.0 18.650326027182025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAACC 20 0.0067955703 52.5 3 GTGGAGC 20 0.0067955703 52.5 67 CTGCCGA 70 0.0011610324 25.0 70 GCTGCCG 80 0.0025260583 21.875 69 CGCTGCC 85 0.0035912225 20.588236 68 ACGCTGC 90 0.0050004628 19.444443 67 TCTGACG 155 0.0068602795 13.548387 63 CATCTGA 160 0.008464588 13.125 61 >>END_MODULE