FastQCFastQC Report
Wed 25 May 2016
SRR1780544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12729
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA160.12569722680493361No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG200.006795570352.51
GCCCACG800.002526058321.87570
AGCCCAC850.003591222520.58823669
GAGCCCA950.00683477418.42105368
CGAGCCC1000.00918779317.567
CTCCGAG1300.0021276816.15384764
ATCTCCG1500.00551967814.062
TCTCCGA1500.00551967814.063
CATCTCC1550.006860279513.54838761
ACATCTC1600.00846458813.12560
Produced by FastQC (version 0.11.5)