##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12729 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26262864325556 34.0 34.0 34.0 27.0 34.0 2 33.0470578992851 34.0 34.0 34.0 32.0 34.0 3 33.260821745620234 34.0 34.0 34.0 33.0 34.0 4 33.18398931573572 34.0 34.0 34.0 32.0 34.0 5 33.18611045643806 34.0 34.0 34.0 32.0 34.0 6 36.86047607824652 38.0 37.0 38.0 35.0 38.0 7 36.544347552832114 38.0 37.0 38.0 34.0 38.0 8 36.39940293817268 38.0 37.0 38.0 34.0 38.0 9 36.319113834551025 38.0 37.0 38.0 34.0 38.0 10-11 35.922617644748215 38.0 36.5 38.0 32.0 38.0 12-13 35.86754654725431 38.0 36.0 38.0 31.5 38.0 14-15 36.105075025532244 38.0 36.5 38.0 33.0 38.0 16-17 36.298609474428474 38.0 37.0 38.0 34.0 38.0 18-19 36.24723073297196 38.0 37.0 38.0 34.0 38.0 20-21 36.0031817110535 38.0 36.5 38.0 32.5 38.0 22-23 35.96028753240632 38.0 36.5 38.0 32.5 38.0 24-25 35.197108963783485 37.5 35.5 38.0 29.0 38.0 26-27 35.482245266713804 37.5 36.0 38.0 31.0 38.0 28-29 35.25390839814597 37.5 35.5 38.0 29.0 38.0 30-31 34.82842328541126 37.0 34.5 38.0 27.0 38.0 32-33 35.480241967161604 37.5 36.0 38.0 31.0 38.0 34-35 35.534802419671614 37.5 36.0 38.0 31.0 38.0 36-37 35.75913268913504 38.0 36.5 38.0 31.5 38.0 38-39 35.56976196087673 38.0 36.5 38.0 29.5 38.0 40-41 34.74412758268521 37.0 34.5 38.0 26.5 38.0 42-43 35.167845078167964 37.0 35.5 38.0 29.0 38.0 44-45 35.37638463351402 37.5 36.0 38.0 31.0 38.0 46-47 35.7046115170084 38.0 36.0 38.0 31.0 38.0 48-49 35.49281168984209 38.0 36.0 38.0 31.0 38.0 50-51 35.7784586377563 38.0 36.0 38.0 31.5 38.0 52-53 35.59026632099929 38.0 36.0 38.0 31.0 38.0 54-55 35.783643648362 38.0 36.0 38.0 31.5 38.0 56-57 35.89123261843035 38.0 37.0 38.0 32.0 38.0 58-59 36.01716552753555 38.0 37.0 38.0 33.5 38.0 60-61 35.52415743577657 38.0 36.5 38.0 29.5 38.0 62-63 35.85670516144238 38.0 36.5 38.0 32.0 38.0 64-65 35.88624400974153 38.0 37.0 38.0 32.5 38.0 66-67 35.14380548354152 37.5 36.0 38.0 29.0 38.0 68-69 34.68072904391547 37.0 34.5 38.0 26.5 38.0 70-71 34.75237646319428 37.0 34.5 38.0 27.0 38.0 72-73 34.47969204179432 37.0 34.0 38.0 26.0 38.0 74-75 35.44308272448739 37.0 35.5 38.0 31.0 38.0 76 34.84853484170006 37.0 34.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7134236623199754 1101 2 0.30013164662271663 1101 3 0.8222480649868089 1101 4 0.730818748100198 1101 5 0.6127594422214528 1101 6 -1.9485922751996014 1101 7 0.6617167305264218 1101 8 1.3700157987379598 1101 9 0.6339759465315566 1101 10-11 -0.346650913180234 1101 12-13 1.164062896272597 1101 14-15 1.1440397797615631 1101 16-17 -0.6374566111738389 1101 18-19 0.008875062540980139 1101 20-21 0.24765206353429647 1101 22-23 1.114512524853204 1101 24-25 -1.2769527872111084 1101 26-27 -0.15434704519535103 1101 28-29 0.7311547363415585 1101 30-31 1.5289104398338083 1101 32-33 -0.2976187486090396 1101 34-35 1.1116134457261495 1101 36-37 0.9621282083388678 1101 38-39 0.6876064622046414 1101 40-41 1.8382400639048555 1101 42-43 1.3509265968981197 1101 44-45 1.7904915642722585 1101 46-47 1.1749259654860396 1101 48-49 0.3254856654398637 1101 50-51 0.39946790563352863 1101 52-53 -1.2080175586968238 1101 54-55 -0.41633413112708695 1101 56-57 -0.8988143408495404 1101 58-59 -1.3520188162610154 1101 60-61 -0.5239801660388537 1101 62-63 0.2844487663266051 1101 64-65 0.21383313562788686 1101 66-67 -2.4243991230395565 1101 68-69 -1.0503581497563417 1101 70-71 0.07434851724628544 1101 72-73 -1.912422052842409 1101 74-75 -1.0148111010756722 1101 76 -2.0867067923542137 1102 1 -0.7207868639958157 1102 2 0.20510240685663206 1102 3 0.8544118076768683 1102 4 -0.25456136885886593 1102 5 0.21217464689981114 1102 6 0.5616416429290538 1102 7 0.5783833971930861 1102 8 1.257442699322759 1102 9 1.2874847184613785 1102 10-11 1.3733783265858506 1102 12-13 1.145057048319373 1102 14-15 -0.09060934304545043 1102 16-17 0.7528942660191476 1102 18-19 0.5044890976287064 1102 20-21 0.9786462155810725 1102 22-23 0.3206528757303957 1102 24-25 -5.407800740427483 1102 26-27 -1.6324172206339398 1102 28-29 0.84518982406086 1102 30-31 1.8351969895414086 1102 32-33 0.8727028888178694 1102 34-35 1.0443619837378435 1102 36-37 -2.3602402127137623 1102 38-39 0.29433160840347483 1102 40-41 1.5282985434370246 1102 42-43 1.0146692869566039 1102 44-45 1.8350822075471172 1102 46-47 1.1288733339070944 1102 48-49 -4.160625445671251 1102 50-51 0.06540357814814257 1102 52-53 -0.7928128803342531 1102 54-55 1.259104465364132 1102 56-57 0.897969284881448 1102 58-59 -0.25625858234288756 1102 60-61 -5.253512329781547 1102 62-63 -2.1731535728546802 1102 64-65 0.9886869367974782 1102 66-67 2.2122967833931924 1102 68-69 0.1777120256822471 1102 70-71 -0.1654175646250664 1102 72-73 -6.8488255616143405 1102 74-75 -2.636887124467485 1102 76 0.9337610439030897 1103 1 1.2460323579721475 1103 2 0.8858804388932455 1103 3 0.6942287413153139 1103 4 1.0085965258779765 1103 5 0.8277352876320805 1103 6 0.9849826040274507 1103 7 1.4479486145843907 1103 8 1.513735605501246 1103 9 1.3984687001547442 1103 10-11 1.5730350771579324 1103 12-13 0.2051256982049665 1103 14-15 0.43570644642823453 1103 16-17 0.25747092505805114 1103 18-19 1.4038025987728702 1103 20-21 0.10393225677101015 1103 22-23 1.226831365432922 1103 24-25 -2.7739334635396133 1103 26-27 0.9144935345147971 1103 28-29 1.2673866203995274 1103 30-31 1.6527027103652046 1103 32-33 2.1993619277194654 1103 34-35 0.9425313201222849 1103 36-37 -0.43461092209591357 1103 38-39 0.8675581530258967 1103 40-41 0.8080468271898837 1103 42-43 0.7663855340961874 1103 44-45 1.355105090842315 1103 46-47 1.962969443746914 1103 48-49 -1.3127993587147344 1103 50-51 0.5631152486286908 1103 52-53 0.7895669823659759 1103 54-55 0.17786876742363233 1103 56-57 1.0879006349958686 1103 58-59 0.13046910644429488 1103 60-61 -5.06987388584562 1103 62-63 -0.23608263463957968 1103 64-65 0.9143162274153269 1103 66-67 1.1510839687478835 1103 68-69 2.13080127053351 1103 70-71 2.0731407877776817 1103 72-73 -3.5845235021177757 1103 74-75 -1.334255545520115 1103 76 -4.610861381726199 1104 1 0.7389338664016094 1104 2 0.7700862951487935 1104 3 0.5047877475264926 1104 4 -0.03221980065263352 1104 5 -0.018759832154970013 1104 6 0.3070766590407601 1104 7 0.9661384992338995 1104 8 1.0168411955633587 1104 9 1.7853364907492448 1104 10-11 -0.34976882701243284 1104 12-13 0.5580538259778081 1104 14-15 0.8132574668363972 1104 16-17 0.5547202595744665 1104 18-19 1.4663580557382687 1104 20-21 0.3202144218109382 1104 22-23 0.7454649058055836 1104 24-25 1.415507530249208 1104 26-27 2.022523703103971 1104 28-29 2.413127525457206 1104 30-31 0.7769263128496817 1104 32-33 1.351757668624522 1104 34-35 0.4613866883565336 1104 36-37 1.9136588205837626 1104 38-39 2.1439556685538506 1104 40-41 2.2860858688935224 1104 42-43 1.7222417896418847 1104 44-45 2.229550521188365 1104 46-47 1.845844332832982 1104 48-49 0.5995786359614002 1104 50-51 1.1338783364725202 1104 52-53 1.6247488812124686 1104 54-55 1.8914889981246077 1104 56-57 1.6329316908964842 1104 58-59 0.6562011383875088 1104 60-61 1.1965413759112522 1104 62-63 0.9930655463719589 1104 64-65 1.3187084190745963 1104 66-67 1.2952720787745022 1104 68-69 3.1605262039851496 1104 70-71 2.6361399004662402 1104 72-73 1.749992913143977 1104 74-75 1.1861526177451935 1104 76 0.9756514842897772 1105 1 1.4861509350472488 1105 2 0.5173033637944329 1105 3 0.4333591760979232 1105 4 0.6904147076961564 1105 5 0.36528469474670544 1105 6 0.7685794419721148 1105 7 0.8965652153749062 1105 8 -0.3370549083327461 1105 9 0.7551880677436742 1105 10-11 0.3452682787389634 1105 12-13 -0.31068457847487707 1105 14-15 0.5607064464282345 1105 16-17 0.120119146401926 1105 18-19 1.209632638298558 1105 20-21 0.46608640696863546 1105 22-23 0.3493610097016955 1105 24-25 0.7823906471323241 1105 26-27 0.5691378032895003 1105 28-29 1.3864577666445896 1105 30-31 2.030173066096438 1105 32-33 1.0521287261384344 1105 34-35 -0.02980069568798882 1105 36-37 0.5075827537934146 1105 38-39 0.96538423998242 1105 40-41 0.23344208410687628 1105 42-43 0.5756740716455937 1105 44-45 1.4899865137672137 1105 46-47 1.0499259654860396 1105 48-49 -0.12653453658034408 1105 50-51 1.0636093197749403 1105 52-53 -0.2850377607170316 1105 54-55 1.5419992022062416 1105 56-57 1.1037109116757051 1105 58-59 0.933334719092521 1105 60-61 1.6376359955773054 1105 62-63 1.0331861400639824 1105 64-65 1.0042371760319213 1105 66-67 1.479641281000852 1105 68-69 1.6238842744860804 1105 70-71 2.189247507145275 1105 72-73 1.3640930986727327 1105 74-75 0.018017181752611577 1105 76 1.0976366419892187 1106 1 -2.085356825484901 1106 2 0.48238096911593686 1106 3 0.8357982004881706 1106 4 0.20371847709748891 1106 5 0.19677028232985094 1106 6 0.34341314485459407 1106 7 0.01740778743698712 1106 8 0.8042976415563885 1106 9 -0.5347232404603162 1106 10-11 0.7973192765216695 1106 12-13 1.729781053454161 1106 14-15 -0.5642935535717655 1106 16-17 0.5231124945172212 1106 18-19 1.1572490462808247 1106 20-21 0.5999555865695214 1106 22-23 -0.11516227189476069 1106 24-25 0.2552287846046042 1106 26-27 2.085490353178642 1106 28-29 0.7941628664228659 1106 30-31 1.262989030619714 1106 32-33 0.42019967957524784 1106 34-35 0.41310396063129673 1106 36-37 1.0627379644364225 1106 38-39 0.6422135082751055 1106 40-41 1.3761804433086482 1106 42-43 -0.27576717003954343 1106 44-45 2.137713786494487 1106 46-47 1.4645601118275096 1106 48-49 1.7723013835970463 1106 50-51 0.7473676346308125 1106 52-53 1.3211152326310867 1106 54-55 0.6913894461086798 1106 56-57 1.0510501577954443 1106 58-59 0.7784012379395264 1106 60-61 1.4197867716305197 1106 62-63 1.376589687735816 1106 64-65 -0.7285787884913546 1106 66-67 1.2703840747924176 1106 68-69 2.843950793333086 1106 70-71 1.9395246690078025 1106 72-73 1.5581064024421423 1106 74-75 -0.16352383820304084 1106 76 0.10706014531515962 1107 1 1.5856458845421955 1107 2 0.3945760910671581 1107 3 0.5778036205423689 1107 4 0.4974854147668637 1107 5 -0.04279611333410571 1107 6 0.9125188359115057 1107 7 0.5339389527486418 1107 8 0.24223802096018687 1107 9 0.5395315020871081 1107 10-11 1.4227935312642117 1107 12-13 -0.030381548171845907 1107 14-15 0.1912620019837945 1107 16-17 0.5347656110483854 1107 18-19 1.2255417292076487 1107 20-21 1.2476520635342965 1107 22-23 0.8089569692976539 1107 24-25 0.6869361016777802 1107 26-27 0.45676406591576324 1107 28-29 1.7922658474526756 1107 30-31 1.0150215509449225 1107 32-33 1.9551590291687404 1107 34-35 1.0505023346150395 1107 36-37 2.0537948750055364 1107 38-39 0.8598286844268657 1107 40-41 1.127128952793747 1107 42-43 2.034259930231457 1107 44-45 1.8043804531611585 1107 46-47 1.6832592988193795 1107 48-49 1.489374554328748 1107 50-51 1.6633567945224144 1107 52-53 1.5975379968587262 1107 54-55 1.1114436466506845 1107 56-57 1.134518992483791 1107 58-59 0.9729811837389875 1107 60-61 1.126019833961145 1107 62-63 1.5844487663266023 1107 64-65 0.5527220245167754 1107 66-67 0.7172675436271163 1107 68-69 0.9940862946880955 1107 70-71 1.4354596283573997 1107 72-73 1.8570223916020296 1107 74-75 0.23241112114654783 1107 76 1.0577376520902249 1108 1 -0.03657633768002455 1108 2 0.6991215456126127 1108 3 0.36517735791610306 1108 4 0.5313237986052499 1108 5 0.27437560383761195 1108 6 -0.6405114671187917 1108 7 -0.5807075118978204 1108 8 -1.1097821810600195 1108 9 -0.7448119322563258 1108 10-11 0.6066319151025965 1108 12-13 1.7120426942523892 1108 14-15 0.6061609918827813 1108 16-17 0.05193732822010588 1108 18-19 0.6300871837531048 1108 20-21 0.6479045887868224 1108 22-23 0.4175428278835156 1108 24-25 1.032390647132324 1108 26-27 -0.8967712876195861 1108 28-29 -0.4999058697190435 1108 30-31 0.3142639751873446 1108 32-33 1.3134923625020747 1108 34-35 0.6179265770392774 1108 36-37 0.9166736628843211 1108 38-39 1.7040206036187797 1108 40-41 1.0743511750159698 1108 42-43 1.7006740716455937 1108 44-45 2.4672592410399403 1108 46-47 1.7317441473042265 1108 48-49 1.6575563725105624 1108 50-51 1.5749729561385664 1108 52-53 0.7035986029193353 1108 54-55 0.7238173840244286 1108 56-57 1.2741654571302519 1108 58-59 1.319698355456154 1108 60-61 2.2739996319409386 1108 62-63 1.4195497764276155 1108 64-65 1.0951462669410148 1108 66-67 2.377368553728118 1108 68-69 2.294338819940627 1108 70-71 0.6324293253270881 1108 72-73 1.0118203714000131 1108 74-75 0.4271080908435252 1108 76 1.1430911874437655 1109 1 -1.8115763376800231 1109 2 0.41957609106715665 1109 3 0.883359176097926 1109 4 0.8335965258779794 1109 5 0.7516483311103386 1109 6 0.09585216924484286 1109 7 0.32838339719308607 1109 8 0.6811269098490769 1109 9 0.5756426131982195 1109 10-11 0.6964046423753203 1109 12-13 0.5154517851614813 1109 14-15 0.8732064464282345 1109 16-17 1.004210055492834 1109 18-19 1.2755417292076459 1109 20-21 0.7240409524231879 1109 22-23 0.3561791915198782 1109 24-25 -0.9380638983222198 1109 26-27 0.5873196214713232 1109 28-29 -0.802178596991773 1109 30-31 1.0608548842782568 1109 32-33 1.1884923625020747 1109 34-35 -0.31616433205163474 1109 36-37 1.0496282083388664 1109 38-39 1.09038423998242 1109 40-41 1.2618511750159698 1109 42-43 2.1120377080092396 1109 44-45 0.9502137864944871 1109 46-47 1.6499259654860339 1109 48-49 0.38937455432874657 1109 50-51 0.28974568341129725 1109 52-53 0.10587133019205908 1109 54-55 0.07949920220623596 1109 56-57 0.32757454803934394 1109 58-59 0.36742562818342606 1109 60-61 1.252408722850035 1109 62-63 0.7025043218821594 1109 64-65 1.3110553578501083 1109 66-67 0.5478230991826649 1109 68-69 -0.02952481642301308 1109 70-71 0.7812929616907311 1109 72-73 -0.3040887195090818 1109 74-75 0.45324445447987927 1109 76 0.5771820965346706 1110 1 -0.3699096710133567 1110 2 0.0714991679902397 1110 3 0.7025899453286897 1110 4 0.49577601305746555 1110 5 0.3349816644436743 1110 6 -0.36953244613977176 1110 7 -0.24212942331973153 1110 8 0.6285628072849718 1110 9 1.8455144080700165 1110 10-11 -0.025069716599034564 1110 12-13 0.7773107595204607 1110 14-15 0.9869884977102856 1110 16-17 1.0356203119030951 1110 18-19 -0.6513813477154287 1110 20-21 1.008335824218058 1110 22-23 -1.3277951674544823 1110 24-25 -2.782294667552989 1110 26-27 1.1148837240354226 1110 28-29 -0.3649991098122811 1110 30-31 0.2970728329962 1110 32-33 0.8166974907071989 1110 34-35 0.46845105256375774 1110 36-37 1.5230256442362986 1110 38-39 0.6256406502388288 1110 40-41 -1.1916744660096654 1110 42-43 -1.7238597278881969 1110 44-45 0.16335481213550906 1110 46-47 1.3063362218963022 1110 48-49 1.845784810739005 1110 50-51 -1.1528825217169043 1110 52-53 0.4308713301920619 1110 54-55 0.05161458682162845 1110 56-57 0.5157155736803674 1110 58-59 -0.2569333461755505 1110 60-61 0.6405497484910541 1110 62-63 0.5066709885488265 1110 64-65 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70-71 0.40515659805436854 2116 72-73 0.5913658259454664 2116 74-75 -0.4706191818837553 2116 76 -1.9250906307380546 2117 1 1.7281295446729175 2117 2 0.8769290322436305 2117 3 -0.9489937650785478 2117 4 0.4791847611720925 2117 5 0.6487071546397516 2117 6 0.18702863983307338 2117 7 -0.0098518969245589 2117 8 0.437009262790248 2117 9 -2.655239739742953 2117 10-11 -3.166095357624677 2117 12-13 -4.373518803073811 2117 14-15 -0.6819406123952945 2117 16-17 -1.0737311209777545 2117 18-19 -1.5744582707923485 2117 20-21 -0.3928708122827018 2117 22-23 0.38412036799046234 2117 24-25 -1.7777697806751647 2117 26-27 -2.6347392020580855 2117 28-29 -2.35217859699177 2117 30-31 0.05423723721942508 2117 32-33 -0.6056252845567514 2117 34-35 0.613247432654255 2117 36-37 -0.11875414460230616 2117 38-39 0.744796004688304 2117 40-41 2.3611158808983177 2117 42-43 0.5649788844798209 2117 44-45 0.6671255512003711 2117 46-47 -0.5088975639257285 2117 48-49 -0.35180191625948254 2117 50-51 -1.065401375412229 2117 52-53 0.07793015372146783 2117 54-55 -0.7153537389702294 2117 56-57 0.8136039598040554 2117 58-59 -0.8119861365224565 2117 60-61 0.6443204875559161 2117 62-63 -1.2776427369413668 2117 64-65 -0.8433564068557757 2117 66-67 -5.41541219493498 2117 68-69 -0.30526011054066515 2117 70-71 0.6511459028671993 2117 72-73 2.0459112804909196 2117 74-75 0.253979748597537 2117 76 -1.344876726994741 2118 1 0.3523125512088612 2118 2 0.27235386884493806 2118 3 -2.0110852683465197 2118 4 -0.49140347412202345 2118 5 -1.6650183355563293 2118 6 -1.8652589418662728 2118 7 -0.14383882502913536 2118 8 -0.04665086792870454 2118 9 -5.06046849791289 2118 10-11 -2.1105398020691197 2118 12-13 -2.8637148815051816 2118 14-15 -0.34207133134954404 2118 16-17 -4.859678833396053 2118 18-19 -1.3244582707923485 2118 20-21 -3.0579034920212607 2118 22-23 -0.10770969736901037 2118 24-25 0.12582499056666308 2118 26-27 -6.126569267417565 2118 28-29 -2.10217859699177 2118 30-31 -2.457200671277306 2118 32-33 -2.533729859720154 2118 34-35 -3.471719887607186 2118 36-37 -8.912871791661132 2118 38-39 -3.3957268711286943 2118 40-41 -5.2173154916506945 2118 42-43 -5.287962291990766 2118 44-45 -6.612286213505513 2118 46-47 -3.0611851456250747 2118 48-49 -1.9661810012268077 2118 50-51 -4.558865427699811 2118 52-53 -4.958017558696827 2118 54-55 -3.9163341311270905 2118 56-57 -1.871036563071769 2118 58-59 -3.115907705149901 2118 60-61 -4.218424610483304 2118 62-63 -3.2988845670067235 2118 64-65 -1.1472779754832203 2118 66-67 -0.9382880119284494 2118 68-69 -5.02258037197857 2118 70-71 -2.870095927198161 2118 72-73 -0.2596442750646375 2118 74-75 -2.945366656631222 2118 76 0.9688487632013363 2119 1 0.5788082777045886 2119 2 -4.620808524317457 2119 3 -2.835871593132847 2119 4 -3.4529419356604834 2119 5 -0.8445055150435081 2119 6 0.09200601539868813 2119 7 -1.1908473720376804 2119 8 0.2695884483106141 2119 9 -0.10320354064793236 2119 10-11 -0.5507107422400637 2119 12-13 -1.120125137915437 2119 14-15 -1.9873704766486853 2119 16-17 0.3176715939543726 2119 18-19 -0.49753519386926826 2119 20-21 -1.5173052014229782 2119 22-23 -1.2765131161724312 2119 24-25 0.44847456321624435 2119 26-27 -0.6030649939132928 2119 28-29 -1.4291016739148503 2119 30-31 -2.690106654183289 2119 32-33 -3.157661483651772 2119 34-35 -1.0315489474362423 2119 36-37 -2.220564099353439 2119 38-39 -4.78461576001758 2119 40-41 -1.2429565172917236 2119 42-43 -0.44180844583691936 2119 44-45 0.36848301726371346 2119 46-47 0.7037721193321929 2119 48-49 -1.9875485225943308 2119 50-51 0.1676302987959133 2119 52-53 -0.10759020826947108 2119 54-55 -3.409923874716835 2119 56-57 -5.9714639134991145 2119 58-59 -2.3594974487396456 2119 60-61 -2.1927835848422745 2119 62-63 -2.775380293502451 2119 64-65 -1.044713872919118 2119 66-67 0.3266692530288182 2119 68-69 -1.0247171241153197 2119 70-71 0.2303314232291953 2119 72-73 0.6997574343370729 2119 74-75 1.3965136852491185 2119 76 0.9175667119192852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 2.0 24 13.0 25 11.0 26 24.0 27 44.0 28 82.0 29 157.0 30 261.0 31 395.0 32 645.0 33 990.0 34 1459.0 35 2167.0 36 3198.0 37 3278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.03456673737136 21.965590384162148 11.226333569015633 26.77350930945086 2 19.71875245502396 19.71875245502396 36.703590227040614 23.858904862911462 3 17.6840285961191 24.762353680571923 30.245895199937152 27.30772252337183 4 12.993950820960013 17.95899127975489 41.527221305679944 27.519836593605152 5 12.907533977531621 37.26137167098751 37.14353052085788 12.687563830622986 6 28.572550868096474 40.898735171655275 17.24408830230183 13.284625657946423 7 27.57482913033231 31.30646555110378 23.11257757875717 18.00612773980674 8 24.605232147065756 35.69801241260115 19.97014690863383 19.72660853169927 9 24.00031424306701 14.965826066462409 22.546940058134968 38.486919632335606 10-11 25.70115484327127 24.982323827480556 28.651111634849556 20.66540969439862 12-13 23.898185246288005 24.196716159949723 27.06418414643727 24.840914447325005 14-15 22.480163406394844 25.622594076518183 24.42061434519601 27.476628171890958 16-17 22.421242831330034 28.12868253594155 26.749941079424932 22.70013355330348 18-19 22.719773744991752 27.830151622279836 27.6101814753712 21.839893157357217 20-21 22.150208186031893 27.912640427370572 27.107392568151468 22.82975881844607 22-23 22.213056799434362 27.69267028046194 28.234739571058213 21.85953334904549 24-25 22.213056799434362 27.594469322020583 27.971561002435386 22.220912876109672 26-27 21.788828658967713 28.42721344960327 27.107392568151468 22.676565323277558 28-29 22.2012726844214 27.154529028203317 29.075339775316206 21.568858512059077 30-31 22.319113834551025 27.264514101657632 28.097258229240317 22.319113834551025 32-33 21.62777908712389 28.258307801084136 27.26058606331998 22.853327048471993 34-35 22.515515751433735 26.761725194437897 28.556838714745858 22.16592033938251 36-37 22.739413936680023 27.3273627150601 27.315578600047136 22.617644748212744 38-39 22.401602639641762 27.559116976981695 27.405923481813183 22.63335690156336 40-41 22.649069054913976 26.522114855840996 28.615759289810665 22.213056799434362 42-43 21.615994972110926 27.720166548825514 28.556838714745858 22.1069997643177 44-45 21.3096079817739 27.21344960326813 28.643255558174246 22.83368685678372 46-47 21.443161285254146 26.702804619373083 28.89465001178412 22.959384083588656 48-49 21.058213528164035 27.083824338125538 29.735250216042107 22.122711917668315 50-51 20.826459266242438 27.76337497053971 28.356508759525497 23.053657003692358 52-53 20.861811611281325 26.926702804619374 29.629193180925444 22.582292403173852 54-55 20.229397438919 27.256658024982322 29.562416529185327 22.951528006913346 56-57 20.339382512373323 26.537827009191613 29.6331212192631 23.48966925917197 58-59 20.30795820567209 26.761725194437897 29.193180925445834 23.737135674444183 60-61 20.2726058606332 26.639956005970618 28.745384554953258 24.342053578442925 62-63 18.917432634142507 27.11132060648912 28.914290203472387 25.056956555895987 64-65 19.809097336790007 26.742085002749626 28.38400502788907 25.064812632571293 66-67 19.98193102364679 26.027182025296568 28.164034880980438 25.826852070076207 68-69 19.569486998193103 27.37449917511195 27.81836750726687 25.237646319428077 70-71 19.777673030088774 27.13096079817739 27.358787021761334 25.732579149972505 72-73 19.534134653154215 27.237017833294054 26.663524235996544 26.565323277555187 74-75 19.43593369471286 27.119176683164426 26.958127111320607 26.4867625108021 76 19.451645848063475 27.1898813732422 26.812789692827405 26.545683085866916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 1.5 17 1.0 18 0.5 19 0.5 20 1.5 21 2.0 22 6.5 23 15.0 24 18.0 25 17.0 26 30.0 27 52.5 28 71.0 29 80.0 30 111.0 31 143.0 32 163.5 33 183.0 34 224.0 35 317.0 36 401.5 37 434.0 38 488.0 39 566.0 40 687.5 41 798.5 42 812.0 43 869.5 44 964.5 45 1012.0 46 1022.0 47 960.5 48 880.5 49 838.0 50 814.0 51 757.0 52 608.5 53 474.0 54 431.0 55 418.5 56 311.5 57 206.0 58 195.0 59 154.5 60 85.5 61 50.0 62 43.0 63 26.5 64 6.5 65 2.0 66 1.5 67 2.0 68 1.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12729.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.9211249901799 #Duplication Level Percentage of deduplicated Percentage of total 1 80.31870860927152 60.97886715374342 2 12.903559602649006 19.59305522821903 3 4.00455298013245 9.120905020032996 4 1.5107615894039736 4.587948778380077 5 0.6519039735099338 2.4746641527221303 6 0.33112582781456956 1.5083667216592034 7 0.15521523178807947 0.8248880509073769 8 0.05173841059602649 0.31424306701233407 9 0.010347682119205299 0.07070469007777516 >10 0.06208609271523179 0.5263571372456595 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA 16 0.12569722680493361 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.007856076675308351 0.0 17 0.0 0.0 0.0 0.007856076675308351 0.0 18 0.0 0.0 0.0 0.007856076675308351 0.0 19 0.0 0.0 0.0 0.007856076675308351 0.0 20 0.0 0.0 0.0 0.007856076675308351 0.0 21 0.0 0.0 0.0 0.007856076675308351 0.0 22 0.0 0.0 0.0 0.007856076675308351 0.0 23 0.0 0.0 0.0 0.007856076675308351 0.0 24 0.0 0.0 0.0 0.007856076675308351 0.0 25 0.0 0.0 0.0 0.007856076675308351 0.0 26 0.0 0.0 0.0 0.007856076675308351 0.0 27 0.0 0.0 0.0 0.015712153350616702 0.0 28 0.0 0.0 0.0 0.015712153350616702 0.0 29 0.0 0.0 0.0 0.05499253672715846 0.0 30 0.0 0.0 0.0 0.05499253672715846 0.0 31 0.0 0.0 0.0 0.07070469007777516 0.0 32 0.0 0.0 0.0 0.08641684342839186 0.0 33 0.0 0.0 0.0 0.14926545683085868 0.0 34 0.0 0.0 0.0 0.18068976353209207 0.0 35 0.0 0.0 0.0 0.22782622358394217 0.0 36 0.0 0.0 0.0 0.37709168041480084 0.0 37 0.0 0.0 0.0 0.6677665174012098 0.0 38 0.0 0.0 0.0 1.029146044465394 0.0 39 0.0 0.0 0.0 1.3826694948542697 0.0 40 0.0 0.0 0.0 1.7754733286196873 0.0 41 0.0 0.0 0.0 2.3411108492418884 0.0 42 0.0 0.0 0.0 2.843899756461623 0.0 43 0.0 0.0 0.0 3.315264356980124 0.0 44 0.0 0.0 0.0 3.8573336475764 0.0 45 0.0 0.0 0.0 4.415115091523293 0.0 46 0.0 0.0 0.0 5.027889072197345 0.0 47 0.0 0.0 0.0 5.884201429805955 0.0 48 0.0 0.0 0.0 6.654096943986174 0.0 49 0.0 0.0 0.0 7.368999921439233 0.0 50 0.0 0.0 0.0 8.09961505224291 0.0 51 0.0 0.0 0.0 8.877366643098437 0.0 52 0.0 0.0 0.0 9.474428470421872 0.0 53 0.0 0.0 0.0 10.205043601225547 0.0 54 0.0 0.0 0.0 10.919946578678609 0.0 55 0.0 0.0 0.0 11.587713096079817 0.0 56 0.0 0.0 0.0 12.169062770052635 0.0 57 0.0 0.0 0.0 13.064655511037788 0.0 58 0.0 0.0 0.0 13.638149108335297 0.0 59 0.0 0.0 0.0 14.376620315814282 0.0 60 0.0 0.0 0.0 15.264356980124125 0.0 61 0.0 0.0 0.0 15.932123497525335 0.0 62 0.0 0.0 0.0 16.67059470500432 0.0 63 0.0 0.0 0.0 17.283368685678372 0.0 64 0.0 0.0 0.0 17.982559509780817 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAAG 20 0.0067955703 52.5 1 GCCCACG 80 0.0025260583 21.875 70 AGCCCAC 85 0.0035912225 20.588236 69 GAGCCCA 95 0.006834774 18.421053 68 CGAGCCC 100 0.009187793 17.5 67 CTCCGAG 130 0.00212768 16.153847 64 ATCTCCG 150 0.005519678 14.0 62 TCTCCGA 150 0.005519678 14.0 63 CATCTCC 155 0.0068602795 13.548387 61 ACATCTC 160 0.008464588 13.125 60 >>END_MODULE