FastQCFastQC Report
Wed 25 May 2016
SRR1780543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239894
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT250.002341830635.22205736
CGACCCA250.002353856835.18531432
TTCACGC250.00236351335.155972
TTGGTAA401.8192071E-532.9862333
GCGACCC353.2141572E-431.42201231
CGTTCGG454.0330357E-529.35784524
CCCAACG300.00571256629.35784121
CGCAAAG300.00571256629.35784112
GGTAAAC454.0387902E-529.35171535
TCGTTCG454.05032E-529.33945723
CCAACGT300.005730089729.33945722
CGGTAAG454.0560953E-529.33333428
TTCGGTA454.0618776E-529.32721326
TTGGACG300.00574179629.32721127
ATACTGG454.0850773E-529.3027536
TACCGCA300.00576526529.3027539
CACGCCA300.005771144329.2966444
TTAGAGG554.9876435E-627.9649754
ACGACCC407.0155715E-427.50000228
CGCATCT609.647678E-625.68811215
Produced by FastQC (version 0.11.5)