FastQCFastQC Report
Wed 25 May 2016
SRR1780543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239894
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGA357.270626E-637.7108926
TGCGATG357.270626E-637.7108928
TGTTCCC250.002345279335.21151410
CGCAAAG250.002345279335.21151412
AGAACAA250.00235008435.19683530
GCTACTA250.00235008435.19683544
CTACGGG353.211931E-431.42574338
CCAACGT353.211931E-431.42574322
GCACGGA300.00572679129.34292611
AATTCGG300.00573847829.33069417
CATCGCA300.00573847829.33069426
GTACAAG406.976612E-427.5262181
ACAAATC407.0192886E-427.49752420
GTTAAGC407.0192886E-427.4975243
TGTCGTA407.0192886E-427.49752437
ATACCAT407.0192886E-427.4975246
GCCCAGT450.001395684324.44224419
ATTAGAG450.001395684324.4422443
GACCGGG450.001395684324.44224431
CGACCGG450.001395684324.44224430