FastQCFastQC Report
Wed 25 May 2016
SRR1780540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT96723.92972036624598No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT70717.495669388765155RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC2365.8401385795595155No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1483.662459787181391No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC882.1776787923781242No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT741.8312298935906954No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT541.3363028953229399No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC411.0146003464488988No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT370.9156149467953477No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG350.8661222469685721No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC280.6928977975748577No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT270.6681514476614699No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT260.6434050977480821No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC250.6186587478346944No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT220.5444196980945311No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT210.5196733481811433No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGACGT180.4454342984409799No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC170.4206879485275922No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT150.3711952487008166No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG150.3711952487008166No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG150.3711952487008166No Hit
CTTATATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC130.32170254887404104No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT120.2969561989606533No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT120.2969561989606533No Hit
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG120.2969561989606533No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC110.27220984904726553No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCCCGTATGCCGT110.27220984904726553No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG110.27220984904726553No Hit
TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT100.24746349913387775No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT90.22271714922048996No Hit
CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC90.22271714922048996No Hit
CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT90.22271714922048996No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT90.22271714922048996Illumina PCR Primer Index 4 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC90.22271714922048996No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGGACAATCTCGTATGCCGT90.22271714922048996No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT90.22271714922048996No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG90.22271714922048996No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCACGTATGCCGT80.1979707993071022No Hit
CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT80.1979707993071022No Hit
CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT80.1979707993071022No Hit
ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT80.1979707993071022RNA PCR Primer, Index 15 (95% over 23bp)
CTTATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGT70.17322444939371442No Hit
CTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTTGA70.17322444939371442RNA PCR Primer, Index 15 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGT70.17322444939371442No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT70.17322444939371442Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGTCTT70.17322444939371442RNA PCR Primer, Index 36 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC70.17322444939371442No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGT70.17322444939371442No Hit
CTTATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT60.14847809948032664No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGTCTT60.14847809948032664No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT60.14847809948032664No Hit
CTTATACACATCTCCGAGCCAACGAGACCGCGGACAATCTCGTATGCCGT60.14847809948032664No Hit
ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT50.12373174956693887RNA PCR Primer, Index 36 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG50.12373174956693887No Hit
CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT50.12373174956693887No Hit
CTTATACACATCTCCGAGCCCACGTGACCGCGGACAATCTCGTATGCCGT50.12373174956693887No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAAATCGTATGCCGTCTT50.12373174956693887No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCGGACAATCTCGTATGCCGT50.12373174956693887No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT50.12373174956693887No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC50.12373174956693887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCAC150.002065878870.0000117
GCGCCCA150.002065878870.0000116
TCCGCGC150.002065878870.0000113
TCTCCGC150.002065878870.0000111
CGCGCCC150.002065878870.0000115
CTCCGCG150.002065878870.0000112
CCGCGCC150.002065878870.0000114
CTTTACA750.070.01
TTTACAC750.070.02
CTTATAC1700.065.882351
TTACACA850.061.76473
TTATACA1850.060.5405432
TATACAC2100.055.0000043
ACACAAA200.006458767752.570
ATACACA2950.037.9661034
GACAATC3450.031.44927633
ACAATCT3450.031.44927634
AAAAACC450.00381976731.11111364
GGACAAT3500.031.032
AATCTCG3400.030.8823536
Produced by FastQC (version 0.11.5)