##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780540_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4041 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61370947785202 34.0 34.0 34.0 31.0 34.0 2 32.890373669883694 34.0 34.0 34.0 31.0 34.0 3 33.11878247958426 34.0 34.0 34.0 32.0 34.0 4 33.42860678049988 34.0 34.0 34.0 33.0 34.0 5 33.32145508537491 34.0 34.0 34.0 33.0 34.0 6 37.20588963127938 38.0 37.0 38.0 36.0 38.0 7 36.750061865874784 38.0 37.0 38.0 34.0 38.0 8 36.73570898292502 38.0 37.0 38.0 35.0 38.0 9 36.39173471912893 38.0 37.0 38.0 34.0 38.0 10-11 36.056421677802525 38.0 36.5 38.0 32.5 38.0 12-13 35.783221974758725 38.0 36.0 38.0 31.5 38.0 14-15 35.68745360059391 37.5 36.0 38.0 31.0 38.0 16-17 35.61284335560505 38.0 36.0 38.0 31.0 38.0 18-19 35.58475624845335 38.0 35.5 38.0 31.0 38.0 20-21 35.536624597871814 38.0 35.5 38.0 31.0 38.0 22-23 35.52796337540213 37.5 35.0 38.0 32.5 38.0 24-25 34.184978965602575 37.0 33.5 38.0 24.5 38.0 26-27 34.448032665181884 37.0 34.0 38.0 28.0 38.0 28-29 34.3513981687701 37.0 34.5 38.0 27.0 38.0 30-31 33.219747587230884 37.0 32.0 38.0 22.0 38.0 32-33 33.9524870081663 37.0 34.0 38.0 24.5 38.0 34-35 33.03761445186835 36.5 32.5 38.0 22.0 38.0 36-37 33.015961395694134 37.0 33.5 38.0 21.0 38.0 38-39 29.976738431081415 34.0 26.0 37.0 11.0 38.0 40-41 31.87824795842613 35.5 30.5 37.0 16.0 38.0 42-43 33.92279138827023 37.0 34.0 38.0 25.0 38.0 44-45 34.651818856718634 37.0 34.0 38.0 27.0 38.0 46-47 34.44135115070527 37.0 34.0 38.0 25.0 38.0 48-49 33.99022519178421 37.0 33.5 38.0 25.0 38.0 50-51 33.80598861667904 37.0 33.5 38.0 24.0 38.0 52-53 33.32256867112101 37.0 34.0 38.0 22.5 38.0 54-55 33.775921801534274 37.0 34.0 38.0 24.0 38.0 56-57 33.897797574857705 37.0 34.0 38.0 24.0 38.0 58-59 33.36253402623113 37.0 34.0 38.0 24.0 38.0 60-61 33.19166048007919 37.0 34.0 38.0 22.5 38.0 62-63 34.34075723830735 37.0 34.0 38.0 26.0 38.0 64-65 34.470799307102205 37.0 34.5 38.0 26.0 38.0 66-67 32.1201435288295 36.0 30.0 38.0 16.0 38.0 68-69 27.952610739915862 32.5 20.5 37.0 11.0 38.0 70-71 23.59972779015095 24.5 11.0 33.5 10.0 37.0 72-73 19.51410541945063 19.5 11.0 25.0 10.0 33.5 74-75 18.74733976738431 19.0 11.0 25.0 10.0 33.0 76 19.205147240781983 19.0 11.0 25.0 10.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5716751793957613 1101 2 -0.3496259588364907 1101 3 -1.5881301425612548 1101 4 -0.2855208645843348 1101 5 0.5908422674676572 1101 6 -1.957409231635225 1101 7 -0.9379769396410254 1101 8 0.9025739031350497 1101 9 -1.8314906713513466 1101 10-11 -0.5998016785845763 1101 12-13 -0.4210223546233536 1101 14-15 1.5017135738788312 1101 16-17 -2.2198001044363167 1101 18-19 -2.7784227554351304 1101 20-21 0.7797543110913807 1101 22-23 1.4871009902550156 1101 24-25 0.10225955352697014 1101 26-27 0.5396018788143451 1101 28-29 -1.1047447886650659 1101 30-31 -1.7993002525510207 1101 32-33 -2.4132087157338695 1101 34-35 0.515946327923885 1101 36-37 2.0282581432155737 1101 38-39 -2.8769748171934744 1101 40-41 0.8845516153952708 1101 42-43 -0.7274734252675472 1101 44-45 1.0504507253152724 1101 46-47 2.5671628165436218 1101 48-49 -1.4065942152532642 1101 50-51 -3.8737378505567364 1101 52-53 -3.4643768909539006 1101 54-55 -0.06600896630890674 1101 56-57 -0.1389438004376089 1101 58-59 -2.7994719569216535 1101 60-61 -1.899045055391806 1101 62-63 -5.460962768741414 1101 64-65 -4.6751688724502145 1101 66-67 -1.3707714305818008 1101 68-69 2.594510912419185 1101 70-71 1.7866251889665214 1101 72-73 -1.315995934320231 1101 74-75 -0.019877868949077282 1101 76 3.8064819232195397 1102 1 0.3413721490927273 1102 2 0.5291619199513917 1102 3 -0.5093422637733767 1102 4 -0.10976328882676256 1102 5 0.7726604492858371 1102 6 0.19410591987993087 1102 7 0.958992757328673 1102 8 0.7753011758623245 1102 9 0.7563881165274395 1102 10-11 0.1789862002033047 1102 12-13 2.4456443120433136 1102 14-15 0.5320166041818624 1102 16-17 0.901412016775808 1102 18-19 0.33066815365577895 1102 20-21 1.7100573413944034 1102 22-23 1.2355858387398584 1102 24-25 -4.103801052533637 1102 26-27 -2.1755496363371734 1102 28-29 1.04677036285009 1102 30-31 2.4522148989641366 1102 32-33 1.538306435781287 1102 34-35 3.649279661257225 1102 36-37 -2.035378220420789 1102 38-39 -0.7284899687086259 1102 40-41 3.2542485850922382 1102 42-43 3.6058599080657814 1102 44-45 2.7716628465273914 1102 46-47 1.2004961498769546 1102 48-49 -1.5156851243441736 1102 50-51 1.438383361564476 1102 52-53 -0.5098314364084473 1102 54-55 1.2915667912668525 1102 56-57 1.9337834722896545 1102 58-59 2.1702250127753118 1102 60-61 -5.9960147523615035 1102 62-63 -2.4852051929838375 1102 64-65 1.3642250669437246 1102 66-67 5.226198266387897 1102 68-69 -4.699428481520208 1102 70-71 -1.4406475383062052 1102 72-73 -0.2947838131081113 1102 74-75 1.2164857674145608 1102 76 2.3276940444316594 1103 1 0.6198035216417495 1103 2 1.195828586618056 1103 3 0.5455596970109369 1103 4 0.5647465150948037 1103 5 0.5961898610505401 1103 6 -0.08040388404163679 1103 7 1.2491888357600445 1103 8 -0.7070517653141479 1103 9 1.0936430184882227 1103 10-11 -0.5543471331300296 1103 12-13 -0.36219882521158553 1103 14-15 0.012408761044603978 1103 16-17 -0.14760759106733445 1103 18-19 1.2777269771851891 1103 20-21 -1.5624916782134335 1103 22-23 0.4336250544261375 1103 24-25 -3.6116441897885387 1103 26-27 0.4754307558196871 1103 28-29 -0.13950414695383273 1103 30-31 2.3718227421013864 1103 32-33 2.375561337742063 1103 34-35 1.751240445570943 1103 36-37 -0.423613514538431 1103 38-39 0.510725717565883 1103 40-41 1.81503289881773 1103 42-43 0.3901736335559818 1103 44-45 2.7697020622136677 1103 46-47 2.9201039930142088 1103 48-49 -3.2862733596382867 1103 50-51 -0.4165185992198417 1103 52-53 1.6078156224150888 1103 54-55 -0.7103939930468712 1103 56-57 0.8102540605249544 1103 58-59 -1.6042847911462488 1103 60-61 -4.64503436020464 1103 62-63 0.0069516697612641565 1103 64-65 1.391676047335885 1103 66-67 -0.9777233022395535 1103 68-69 2.190767596911165 1103 70-71 -1.5877063618356182 1103 72-73 -1.9790975385983067 1103 74-75 0.08707400270867538 1103 76 1.908086201294406 1104 1 1.1524832602038373 1104 2 1.195828586618056 1104 3 0.9573244028932919 1104 4 0.6235700445065717 1104 5 0.7726604492858371 1104 6 0.6941059198799309 1104 7 1.20343720177312 1104 8 1.175301175862323 1104 9 1.6230547831941067 1104 10-11 0.6956528668699704 1104 12-13 0.8289776453766464 1104 14-15 2.309794381959634 1104 16-17 2.1236342389980223 1104 18-19 2.130668153655776 1104 20-21 0.47672400806106907 1104 22-23 0.7244747276287526 1104 24-25 2.2739767252441396 1104 26-27 2.8300059192183795 1104 28-29 3.3801036961834257 1104 30-31 2.8799926767419137 1104 32-33 1.7383064357812756 1104 34-35 1.8770574390349992 1104 36-37 4.3035106684681 1104 38-39 5.360398920180263 1104 40-41 3.7986930295366754 1104 42-43 1.57808213028801 1104 44-45 2.421662846527397 1104 46-47 2.789385038765843 1104 48-49 1.2676482089891579 1104 50-51 2.505050028231146 1104 52-53 4.184613008035996 1104 54-55 2.247122346822408 1104 56-57 1.7448945834007716 1104 58-59 1.475780568330869 1104 60-61 2.098429692082945 1104 62-63 2.5036836959050532 1104 64-65 3.0420028447215017 1104 66-67 2.159531599721234 1104 68-69 5.089460407368687 1104 70-71 3.448241350582684 1104 72-73 -1.5558949242192206 1104 74-75 -1.2724031214743299 1104 76 -1.18341706667945 1105 1 1.2080388157593944 1105 2 1.1402730310624989 1105 3 0.9573244028932919 1105 4 0.01245893339545745 1105 5 -0.44956177293638433 1105 6 0.5829948087688166 1105 7 1.3701038684397844 1105 8 0.5086345091956588 1105 9 0.289721449860771 1105 10-11 1.1400973113144133 1105 12-13 0.7178665342655322 1105 14-15 1.2264610486263052 1105 16-17 0.7347453501091366 1105 18-19 1.4917792647668904 1105 20-21 1.2822795636166262 1105 22-23 1.2244747276287526 1105 24-25 1.1628656141330254 1105 26-27 1.302228141440601 1105 28-29 1.9078814739611971 1105 30-31 1.5188815656307995 1105 32-33 1.0716397691146184 1105 34-35 0.3215018834794421 1105 36-37 3.220177335134771 1105 38-39 0.777065586846927 1105 40-41 1.770915251758904 1105 42-43 2.772526574732453 1105 44-45 2.1438850687496114 1105 46-47 2.5116072609880646 1105 48-49 0.5176482089891579 1105 50-51 2.838383361564482 1105 52-53 1.7123907858137741 1105 54-55 0.7749001246001868 1105 56-57 0.41156125006743594 1105 58-59 1.5035583461086546 1105 60-61 2.4595408031940593 1105 62-63 0.6703503625717246 1105 64-65 2.514225066943723 1105 66-67 2.215087155276791 1105 68-69 1.7005715184797943 1105 70-71 -0.2739808716395373 1105 72-73 -0.500339368663667 1105 74-75 1.949819100747895 1105 76 -1.7945281777905642 1106 1 0.9223245300451097 1106 2 0.19582858661805602 1106 3 0.4573244028932919 1106 4 0.6235700445065717 1106 5 -0.8701966935713088 1106 6 -1.0677988420248354 1106 7 0.2828022811381956 1106 8 0.3895868901480384 1106 9 -2.2340880739487474 1106 10-11 1.2313671525842622 1106 12-13 1.6861205025195005 1106 14-15 -0.27750720534194784 1106 16-17 1.0918882072519978 1106 18-19 0.3449538679414843 1106 20-21 -0.7494664681293983 1106 22-23 1.0260620292160567 1106 24-25 -0.49189629062887263 1106 26-27 0.3220694112818663 1106 28-29 -0.6079915419118151 1106 30-31 0.16173870848793825 1106 32-33 0.7264016738765164 1106 34-35 0.33737489935245435 1106 36-37 3.8074789224363528 1106 38-39 -4.1951566353752945 1106 40-41 2.520915251758904 1106 42-43 1.2010980033038834 1106 44-45 1.076424751289295 1106 46-47 2.28144853082933 1106 48-49 3.2557434470843987 1106 50-51 0.13600240918352569 1106 52-53 0.3830257064486986 1106 54-55 -1.6536713039712438 1106 56-57 0.562354900861088 1106 58-59 0.15832025087055257 1106 60-61 1.201604295257539 1106 62-63 0.3052709974923573 1106 64-65 0.026129828848489467 1106 66-67 1.8500077901974166 1106 68-69 5.581523899432174 1106 70-71 -0.8335046811633511 1106 72-73 -0.14716476548906243 1106 74-75 0.4379143388431288 1106 76 -2.0961154793778647 1107 1 1.1455388157593944 1107 2 -0.929171413381944 1107 3 0.6448244028932919 1107 4 0.6235700445065717 1107 5 0.7726604492858371 1107 6 0.6107725865465952 1107 7 1.3006594239953415 1107 8 0.8628011758623231 1107 9 -0.12694521680589332 1107 10-11 1.1644028668699704 1107 12-13 0.2977276453766464 1107 14-15 0.709099937515191 1107 16-17 1.5472453501091366 1107 18-19 2.068168153655776 1107 20-21 2.1121406747277405 1107 22-23 1.2314191720731955 1107 24-25 0.6732822807996968 1107 26-27 1.826533696996158 1107 28-29 2.2134370295167543 1107 30-31 2.3938815656307995 1107 32-33 3.373723102447947 1107 34-35 2.140946327923885 1107 36-37 4.289621779579214 1107 38-39 0.6485933646247055 1107 40-41 -1.104084748241096 1107 42-43 0.5225265747324528 1107 44-45 1.4424961798607256 1107 46-47 2.5671628165436218 1107 48-49 1.7155648756558293 1107 50-51 1.6717166948978104 1107 52-53 0.5214185635915527 1107 54-55 2.3686501246001868 1107 56-57 2.3212834722896574 1107 58-59 2.024391679441983 1107 60-61 3.299818580971831 1107 62-63 2.385628140349496 1107 64-65 2.941308400277059 1107 66-67 0.43036493305456247 1107 68-69 3.0859881851464586 1107 70-71 -0.0031475383062051776 1107 72-73 0.9510495202252223 1107 74-75 -0.3210142325854406 1107 76 -3.426472622235007 1108 1 0.47470548242606014 1108 2 0.9291619199513903 1108 3 0.2906577362266276 1108 4 -0.10976328882676256 1108 5 0.30599378261916854 1108 6 -0.20589408012007482 1108 7 0.6923260906620072 1108 8 -0.4913654908043412 1108 9 -1.3769452168058933 1108 10-11 0.6456528668699733 1108 12-13 2.0789776453766464 1108 14-15 0.4986832708485238 1108 16-17 -0.49858798322419773 1108 18-19 1.0640014869891132 1108 20-21 1.8767240080610748 1108 22-23 0.36891917207319835 1108 24-25 2.0295322807996996 1108 26-27 1.357783696996158 1108 28-29 0.1801036961834228 1108 30-31 0.3188815656307966 1108 32-33 1.53830643578128 1108 34-35 1.3492796612572135 1108 36-37 1.8312884462458854 1108 38-39 1.7715100312913705 1108 40-41 2.087581918425567 1108 42-43 0.2058599080657899 1108 44-45 2.5716628465273885 1108 46-47 0.9671628165436275 1108 48-49 1.6509815423224978 1108 50-51 1.8717166948978132 1108 52-53 0.156835230258217 1108 54-55 0.8915667912668539 1108 56-57 0.6337834722896574 1108 58-59 2.336891679441983 1108 60-61 2.3706519143051636 1108 62-63 2.4147948070161647 1108 64-65 1.6308917336103903 1108 66-67 2.7261982663878968 1108 68-69 -0.06609514818687146 1108 70-71 1.6593524616937962 1108 72-73 1.5385495202252208 1108 74-75 0.8498191007478937 1108 76 0.794360711098328 1109 1 1.136610244330825 1109 2 1.195828586618056 1109 3 0.6716101171790072 1109 4 0.6235700445065717 1109 5 0.4869461635715524 1109 6 0.43220115797516456 1109 7 -0.002912004576089089 1109 8 0.3895868901480384 1109 9 1.1230547831941067 1109 10-11 0.374224295441401 1109 12-13 1.4718347882337852 1109 14-15 1.5439213660866216 1109 16-17 2.1276024929662753 1109 18-19 1.7378110107986373 1109 20-21 0.6791049604420252 1109 22-23 1.0260620292160567 1109 24-25 -0.13475343348601854 1109 26-27 0.6077836969961581 1109 28-29 -8.486847689610499E-4 1109 30-31 1.4831672799165148 1109 32-33 1.8692588167336623 1109 34-35 1.5873748993524543 1109 36-37 3.5931932081506446 1109 38-39 1.3048433646247055 1109 40-41 1.3423438231874734 1109 42-43 1.4510980033038834 1109 44-45 1.2549961798607256 1109 46-47 0.46001995940076057 1109 48-49 1.7557434470843987 1109 50-51 -1.5068547336736202 1109 52-53 0.0973114207344139 1109 54-55 -1.4750998753998132 1109 56-57 0.45521204371822677 1109 58-59 -2.555965463415159 1109 60-61 -0.9412528475995998 1109 62-63 -0.3018718596505039 1109 64-65 1.4189869717056283 1109 66-67 2.278579218768847 1109 68-69 -3.204190386282111 1109 70-71 2.77363817597951 1109 72-73 -0.825736194060493 1109 74-75 0.8664857674145594 1109 76 1.261027377764993 1110 1 -0.22053261281203618 1110 2 -1.804171413381944 1110 3 0.8858958314647225 1110 4 -0.3050013840648589 1110 5 0.5583747350001218 1110 6 0.28934401511802577 1110 7 0.8542308525667721 1110 8 0.6753011758623231 1110 9 0.9801976403369608 1110 10-11 -2.23291856170146 1110 12-13 -0.17102235462335358 1110 14-15 0.40106422322948276 1110 16-17 0.12760249296627535 1110 18-19 -1.5836175606299392 1110 20-21 -0.4280378967008289 1110 22-23 -3.9739379707839504 1110 24-25 -3.063324862057449 1110 26-27 -0.6065020172895572 1110 28-29 -1.5722772561975304 1110 30-31 -3.159689862940631 1110 32-33 -0.7735983261234836 1110 34-35 -1.0197679577903997 1110 36-37 1.6289074938649293 1110 38-39 0.16198622176756317 1110 40-41 -4.764799033955381 1110 42-43 -1.5489019966961166 1110 44-45 1.7549961798607256 1110 46-47 -2.968551469170663 1110 48-49 1.0057434470843987 1110 50-51 0.7074309806121022 1110 52-53 -0.5812600078370167 1110 54-55 -2.1179570182569556 1110 56-57 -2.9019308134246273 1110 58-59 -1.6988226062723015 1110 60-61 -1.1912528475995998 1110 62-63 -3.1947290025076462 1110 64-65 -0.5095844568658023 1110 66-67 -1.2571350669454375 1110 68-69 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-2.5389726222350077 2117 1 1.2080388157593944 2117 2 0.445828586618056 2117 3 0.9573244028932919 2117 4 0.6235700445065717 2117 5 0.7726604492858371 2117 6 0.6107725865465952 2117 7 1.4256594239953415 2117 8 1.175301175862323 2117 9 1.3730547831941067 2117 10-11 -1.9293471331300296 2117 12-13 -0.6710223546233536 2117 14-15 0.615349937515191 2117 16-17 -0.39025464989086345 2117 18-19 -1.994331846344224 2117 20-21 2.2683906747277405 2117 22-23 2.6689191720731955 2117 24-25 1.0795322807996968 2117 26-27 -0.5172163030038419 2117 28-29 -2.9115629704832457 2117 30-31 -4.3561184343692005 2117 32-33 -1.720026897552053 2117 34-35 3.890946327923885 2117 36-37 0.16462177957921398 2117 38-39 4.0548433646247055 2117 40-41 3.020915251758904 2117 42-43 1.8975265747324528 2117 44-45 -1.3700038201392744 2117 46-47 2.3171628165436218 2117 48-49 0.18431487565582927 2117 50-51 -0.4532833051021896 2117 52-53 3.8651685635915527 2117 54-55 3.1499001246001868 2117 56-57 2.6337834722896574 2117 58-59 -1.2881083205580168 2117 60-61 2.987318580971831 2117 62-63 2.948128140349496 2117 64-65 -0.27744159972294113 2117 66-67 3.4928649330545625 2117 68-69 -1.1327618148535414 2117 70-71 -3.065647538306205 2117 72-73 -3.7364504797747777 2117 74-75 0.2414857674145594 2117 76 1.761027377764993 2118 1 0.608038815759393 2118 2 -0.804171413381944 2118 3 0.9573244028932919 2118 4 0.6235700445065717 2118 5 0.7726604492858371 2118 6 0.8607725865465952 2118 7 1.2256594239953387 2118 8 0.5753011758623217 2118 9 -2.576945216805896 2118 10-11 -0.154347133130031 2118 12-13 -1.6210223546233564 2118 14-15 1.9653499375151853 2118 16-17 -6.7652546498908634 2118 18-19 -0.06933184634422673 2118 20-21 1.543390674727739 2118 22-23 1.9689191720731927 2118 24-25 -2.870467719200306 2118 26-27 -6.142216303003842 2118 28-29 -0.4865629704832486 2118 30-31 2.2188815656308023 2118 32-33 2.8049731024479527 2118 34-35 1.4159463279238906 2118 36-37 -5.735378220420785 2118 38-39 1.3048433646247055 2118 40-41 0.12091525175890538 2118 42-43 0.17252657473245847 2118 44-45 -1.0950038201392758 2118 46-47 2.167162816543616 2118 48-49 2.4843148756558264 2118 50-51 0.2717166948978047 2118 52-53 -1.0098314364084473 2118 54-55 -4.475099875399813 2118 56-57 -0.6662165277103398 2118 58-59 -2.5631083205580154 2118 60-61 -2.06268141902817 2118 62-63 2.3481281403494947 2118 64-65 -1.0024415997229355 2118 66-67 -0.8071350669454382 2118 68-69 -7.232761814853543 2118 70-71 -1.6406475383062045 2118 72-73 -1.061450479774777 2118 74-75 -5.683514232585441 2118 76 -3.3389726222350067 2119 1 1.2080388157593944 2119 2 -2.470838080048612 2119 3 0.2906577362266276 2119 4 0.6235700445065717 2119 5 0.7726604492858371 2119 6 0.5274392532132595 2119 7 0.09232609066200581 2119 8 0.17530117586232308 2119 9 0.9563881165274424 2119 10-11 0.4456528668699704 2119 12-13 -1.4210223546233536 2119 14-15 0.865349937515191 2119 16-17 -0.7652546498908634 2119 18-19 1.7973348203224404 2119 20-21 0.47672400806106907 2119 22-23 0.1689191720731955 2119 24-25 -0.17046771920030324 2119 26-27 3.191117030329494 2119 28-29 0.04677036285008995 2119 30-31 3.352214898964135 2119 32-33 3.904973102447947 2119 34-35 4.515946327923885 2119 36-37 -7.002044887087454 2119 38-39 4.47151003129137 2119 40-41 0.020915251758903963 2119 42-43 2.939193241399117 2119 44-45 0.17166284652739705 2119 46-47 2.5671628165436218 2119 48-49 2.3509815423224936 2119 50-51 3.338383361564482 2119 52-53 2.8235018969248813 2119 54-55 3.191566791266851 2119 56-57 -1.3662165277103426 2119 58-59 4.170225012775319 2119 60-61 3.737318580971831 2119 62-63 2.948128140349496 2119 64-65 3.4308917336103875 2119 66-67 3.826198266387891 2119 68-69 4.033904851813123 2119 70-71 -4.773980871639541 2119 72-73 1.3052161868918866 2119 74-75 3.1164857674145594 2119 76 8.261027377764993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 5.0 24 9.0 25 27.0 26 52.0 27 94.0 28 146.0 29 235.0 30 293.0 31 435.0 32 580.0 33 728.0 34 823.0 35 509.0 36 87.0 37 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8166295471417966 26.255877258104434 13.585746102449889 59.34174709230389 2 0.3711952487008166 3.414996288047513 84.63251670378618 11.58129175946548 3 0.6186587478346944 29.84409799554566 66.76565206632021 2.771591190299431 4 0.24746349913387775 47.88418708240535 24.993813412521654 26.874536005939127 5 0.5196733481811433 49.022519178421184 46.869586735956446 3.588220737441227 6 0.4949269982677555 50.13610492452363 10.22024251422915 39.14872556297946 7 0.9651076466221231 49.26998267755506 5.468943330858698 44.295966344964114 8 0.6928977975748577 48.973026478594406 26.255877258104434 24.078198465726306 9 0.5196733481811433 23.979213066072756 3.365503588220738 72.13560999752536 10-11 0.6557782727047761 28.92848304875031 41.32640435535758 29.089334323187334 12-13 13.325909428359317 3.761445186834942 35.07795100222717 47.83469438257857 14-15 21.083890126206384 15.05815392229646 8.166295471417966 55.691660480079186 16-17 33.8035139816877 28.396436525612472 0.9527344716654293 36.8473150210344 18-19 28.681019549616433 21.07151695124969 0.5196733481811433 49.727790150952735 20-21 15.986142044048504 9.45310566691413 0.7671368473150211 73.79361544172235 22-23 21.26948775055679 40.150952734471666 0.6310319227913883 37.948527592180156 24-25 33.667409057164065 43.97426379609007 1.0393466963622866 21.318980450383567 26-27 40.893343231873295 35.585251175451624 1.0146003464488988 22.50680524622618 28-29 23.620390992328634 9.948032665181886 1.323929720366246 65.10764662212324 30-31 47.018064835436775 14.13016580054442 1.2991833704528584 37.55258599356595 32-33 55.0730017322445 21.281860925513488 1.6085127443702056 22.036624597871814 34-35 11.098737936154416 47.66146993318486 12.830982430091561 28.408809700569165 36-37 1.2744370205394704 55.840138579559515 20.551843603068548 22.333580796832468 38-39 13.437268002969564 13.635238802276664 31.87329868844345 41.054194506310324 40-41 8.62410294481564 14.92204899777283 41.72234595397179 34.731502103439745 42-43 38.20836426627072 8.883939618906211 44.17223459539718 8.735461519425884 44-45 11.036872061370948 26.09502598366741 35.54813165058154 27.319970304380103 46-47 39.65602573620391 3.637713437268003 21.10863647611977 35.59762435040831 48-49 30.252412769116553 1.3363028953229399 21.54169759960406 46.869586735956446 50-51 8.092056421677803 1.7074981440237564 47.45112595892106 42.74931947537738 52-53 13.152684978965604 1.682751794110369 68.68349418460778 16.481069042316257 54-55 8.426132145508538 1.893095768374165 50.04949269982678 39.63127938629052 56-57 38.084632516703785 2.078693392724573 29.63375402128186 30.202920069289778 58-59 10.752289037366989 27.629299678297453 52.59836674090571 9.020044543429844 60-61 25.600098985399654 61.18535016085127 10.739915862410294 2.4746349913387777 62-63 2.845830240039594 94.02375649591686 1.0022271714922049 2.1281860925513487 64-65 0.3093293739173472 96.73348181143281 0.779510022271715 2.1776787923781242 66-67 0.4083147735708983 93.62781489730266 1.0764662212323683 4.887404107894086 68-69 0.9279881217520415 84.42217272952239 2.6849789656025735 11.96486018312299 70-71 2.3014105419450632 73.95446671615936 5.419450631031922 18.32467211086365 72-73 3.34075723830735 60.467706013363035 9.06953724325662 27.121999505073003 74-75 4.936896807720862 58.401385795595154 8.648849294729027 28.012868101954965 76 3.8109378866617174 62.212323682256866 6.755753526354863 27.220984904726553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.0 23 0.5 24 1.0 25 2.0 26 1.0 27 0.0 28 0.5 29 1.0 30 0.5 31 1.5 32 1.5 33 0.0 34 1.0 35 3.0 36 3.5 37 3.0 38 4.5 39 10.5 40 25.5 41 99.5 42 163.0 43 378.0 44 746.0 45 893.5 46 888.0 47 784.5 48 535.0 49 292.5 50 196.0 51 141.0 52 59.0 53 22.5 54 13.0 55 12.0 56 8.0 57 5.5 58 6.0 59 4.5 60 1.5 61 0.0 62 0.0 63 0.0 64 0.5 65 0.5 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 4041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.79584261321455 #Duplication Level Percentage of deduplicated Percentage of total 1 82.23552894211576 20.390992328631526 2 7.684630738522954 3.8109378866617174 3 2.5948103792415167 1.9302152932442462 4 1.4970059880239521 1.4847809948032664 5 0.7984031936127743 0.989853996535511 6 0.39920159680638717 0.5939123979213066 7 0.6986027944111777 1.212571145756001 8 0.39920159680638717 0.7918831972284088 9 0.7984031936127743 1.7817371937639197 >10 2.19560878243513 10.739915862410294 >50 0.29940119760479045 5.3452115812917596 >100 0.19960079840319359 9.502598366740905 >500 0.19960079840319359 41.42538975501114 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 967 23.92972036624598 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 707 17.495669388765155 RNA PCR Primer, Index 15 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 236 5.8401385795595155 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 148 3.662459787181391 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 88 2.1776787923781242 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT 74 1.8312298935906954 No Hit TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 54 1.3363028953229399 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 41 1.0146003464488988 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT 37 0.9156149467953477 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 35 0.8661222469685721 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 28 0.6928977975748577 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT 27 0.6681514476614699 No Hit CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT 26 0.6434050977480821 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 25 0.6186587478346944 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT 22 0.5444196980945311 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT 21 0.5196733481811433 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGACGT 18 0.4454342984409799 No Hit CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 17 0.4206879485275922 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT 15 0.3711952487008166 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG 15 0.3711952487008166 No Hit CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 15 0.3711952487008166 No Hit CTTATATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 13 0.32170254887404104 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT 12 0.2969561989606533 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT 12 0.2969561989606533 No Hit CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 12 0.2969561989606533 No Hit TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 11 0.27220984904726553 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCCCGTATGCCGT 11 0.27220984904726553 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 11 0.27220984904726553 No Hit TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 10 0.24746349913387775 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT 9 0.22271714922048996 No Hit CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 9 0.22271714922048996 No Hit CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT 9 0.22271714922048996 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT 9 0.22271714922048996 Illumina PCR Primer Index 4 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC 9 0.22271714922048996 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGGACAATCTCGTATGCCGT 9 0.22271714922048996 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT 9 0.22271714922048996 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG 9 0.22271714922048996 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCACGTATGCCGT 8 0.1979707993071022 No Hit CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 8 0.1979707993071022 No Hit CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 8 0.1979707993071022 No Hit ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT 8 0.1979707993071022 RNA PCR Primer, Index 15 (95% over 23bp) CTTATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGT 7 0.17322444939371442 No Hit CTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTTGA 7 0.17322444939371442 RNA PCR Primer, Index 15 (96% over 28bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGT 7 0.17322444939371442 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT 7 0.17322444939371442 Illumina PCR Primer Index 7 (95% over 21bp) ATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGTCTT 7 0.17322444939371442 RNA PCR Primer, Index 36 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC 7 0.17322444939371442 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGT 7 0.17322444939371442 No Hit CTTATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 6 0.14847809948032664 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGTCTT 6 0.14847809948032664 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT 6 0.14847809948032664 No Hit CTTATACACATCTCCGAGCCAACGAGACCGCGGACAATCTCGTATGCCGT 6 0.14847809948032664 No Hit ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT 5 0.12373174956693887 RNA PCR Primer, Index 36 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG 5 0.12373174956693887 No Hit CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT 5 0.12373174956693887 No Hit CTTATACACATCTCCGAGCCCACGTGACCGCGGACAATCTCGTATGCCGT 5 0.12373174956693887 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAAATCGTATGCCGTCTT 5 0.12373174956693887 No Hit CTTATACACATCTCCGCGCCCACGAGACCGCGGACAATCTCGTATGCCGT 5 0.12373174956693887 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT 5 0.12373174956693887 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC 5 0.12373174956693887 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.024746349913387776 0.0 19 0.0 0.0 0.0 0.07423904974016332 0.0 20 0.0 0.0 0.0 0.0989853996535511 0.0 21 0.0 0.0 0.0 0.0989853996535511 0.0 22 0.0 0.0 0.0 0.0989853996535511 0.0 23 0.0 0.0 0.0 0.0989853996535511 0.0 24 0.0 0.0 0.0 0.0989853996535511 0.0 25 0.0 0.0 0.0 0.0989853996535511 0.0 26 0.0 0.0 0.0 0.0989853996535511 0.0 27 0.0 0.0 0.0 0.0989853996535511 0.0 28 0.0 0.0 0.0 0.0989853996535511 0.0 29 0.0 0.0 0.0 0.0989853996535511 0.0 30 0.0 0.0 0.0 0.0989853996535511 0.0 31 0.0 0.0 0.0 0.0989853996535511 0.0 32 0.0 0.0 0.0 0.0989853996535511 0.0 33 0.0 0.0 0.0 0.0989853996535511 0.0 34 0.0 0.0 0.0 0.0989853996535511 0.0 35 0.0 0.0 0.0 0.0989853996535511 0.0 36 0.0 0.0 0.0 0.0989853996535511 0.0 37 0.0 0.0 0.0 0.12373174956693887 0.0 38 0.0 0.0 0.0 0.12373174956693887 0.0 39 0.0 0.0 0.0 0.14847809948032664 0.0 40 0.0 0.0 0.0 0.14847809948032664 0.0 41 0.0 0.0 0.0 0.14847809948032664 0.0 42 0.0 0.0 0.0 0.14847809948032664 0.0 43 0.0 0.0 0.0 0.14847809948032664 0.0 44 0.0 0.0 0.0 0.1979707993071022 0.0 45 0.0 0.0 0.0 0.22271714922049 0.0 46 0.0 0.0 0.0 0.27220984904726553 0.0 47 0.0 0.0 0.0 0.34644889878742885 0.0 48 0.0 0.0 0.0 0.34644889878742885 0.0 49 0.0 0.0 0.0 0.3711952487008166 0.0 50 0.0 0.0 0.0 0.3959415986142044 0.0 51 0.0 0.0 0.0 0.47018064835436774 0.0 52 0.0 0.0 0.0 0.4949269982677555 0.0 53 0.0 0.0 0.0 0.5196733481811433 0.0 54 0.0 0.0 0.0 0.5691660480079188 0.0 55 0.0 0.0 0.0 0.5691660480079188 0.0 56 0.0 0.0 0.0 0.5691660480079188 0.0 57 0.0 0.0 0.0 0.5939123979213066 0.0 58 0.0 0.0 0.0 0.5939123979213066 0.0 59 0.0 0.0 0.0 0.5939123979213066 0.0 60 0.0 0.0 0.0 0.6186587478346944 0.0 61 0.0 0.0 0.0 0.6681514476614699 0.0 62 0.0 0.0 0.0 0.6681514476614699 0.0 63 0.0 0.0 0.0 0.6681514476614699 0.0 64 0.0 0.0 0.0 0.6681514476614699 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCAC 15 0.0020658788 70.00001 17 GCGCCCA 15 0.0020658788 70.00001 16 TCCGCGC 15 0.0020658788 70.00001 13 TCTCCGC 15 0.0020658788 70.00001 11 CGCGCCC 15 0.0020658788 70.00001 15 CTCCGCG 15 0.0020658788 70.00001 12 CCGCGCC 15 0.0020658788 70.00001 14 CTTTACA 75 0.0 70.0 1 TTTACAC 75 0.0 70.0 2 CTTATAC 170 0.0 65.88235 1 TTACACA 85 0.0 61.7647 3 TTATACA 185 0.0 60.540543 2 TATACAC 210 0.0 55.000004 3 ACACAAA 20 0.0064587677 52.5 70 ATACACA 295 0.0 37.966103 4 GACAATC 345 0.0 31.449276 33 ACAATCT 345 0.0 31.449276 34 AAAAACC 45 0.003819767 31.111113 64 GGACAAT 350 0.0 31.0 32 AATCTCG 340 0.0 30.88235 36 >>END_MODULE