FastQCFastQC Report
Wed 25 May 2016
SRR1780539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68464
Sequences flagged as poor quality0
Sequence length50
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG950.13875905585417153No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC740.10808600140219678No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG690.10078289319934565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG207.818028E-443.9846530
ATGACGC207.846253E-443.952529
GTTATAC301.2909895E-436.6538731
TGATCGT250.002341362635.1877231
TAAAGAT250.002341362635.187721
AATGATC250.002341362635.1877229
GGTGGTC751.8189894E-1235.1877244
TATAACA250.00234978335.1620183
CGGTGGT950.032.40974443
TTTCGGT353.1929996E-431.41760843
CACATAT353.2067363E-431.3946594
TTTACAC857.2759576E-1231.0253072
CCGGTGC300.005676637429.36603215
TGTATAT300.00569700529.3445537
CGAGAAT300.00569700529.3445525
TTCGGTG454.0239152E-529.32310144
CTTATAC4800.028.4067521
TCGTGTA406.94848E-427.51051534
TAATAAG406.9782976E-427.4904061
TTATAAC407.0082204E-427.4703242
Produced by FastQC (version 0.11.5)