##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1780539_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68464
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	17.98532075251227	16.0	10.0	26.0	7.0	30.0
2	18.305722715587756	16.0	10.0	27.0	7.0	31.0
3	18.581254381864923	16.0	10.0	28.0	7.0	31.0
4	20.353134494040663	19.0	10.0	32.0	7.0	35.0
5	20.55458343070811	19.0	10.0	32.0	7.0	35.0
6	20.82715003505492	17.0	10.0	32.0	8.0	35.0
7	20.524114863285813	17.0	10.0	31.0	8.0	35.0
8	20.461498013554568	17.0	10.0	31.0	8.0	35.0
9	20.485992638466932	17.0	10.0	31.0	8.0	37.0
10	20.40381806496845	17.0	10.0	31.0	8.0	37.0
11	20.607078172470203	17.0	10.0	31.0	8.0	37.0
12	20.400502453844357	17.0	10.0	31.0	8.0	37.0
13	20.392147698060295	17.0	10.0	31.0	8.0	37.0
14	20.606800654358494	17.0	10.0	31.0	8.0	37.0
15	20.40140803926151	17.0	10.0	31.0	8.0	37.0
16	20.243281140453377	17.0	8.0	31.0	7.0	37.0
17	20.39154884318766	17.0	9.0	31.0	7.0	37.0
18	20.05345875204487	17.0	8.0	30.0	7.0	37.0
19	20.139635428838513	18.0	8.0	30.0	7.0	37.0
20	20.252658331385838	18.0	8.0	31.0	7.0	37.0
21	20.11232180415985	18.0	8.0	30.0	7.0	37.0
22	20.05886305211498	18.0	8.0	30.0	7.0	37.0
23	19.959263262444498	17.0	8.0	30.0	7.0	37.0
24	19.60902079925216	17.0	8.0	30.0	7.0	37.0
25	19.439720144893666	17.0	8.0	30.0	7.0	36.0
26	19.16724117784529	16.0	8.0	30.0	7.0	36.0
27	19.01711848562748	16.0	8.0	29.0	7.0	36.0
28	18.890175858845524	16.0	8.0	28.0	7.0	36.0
29	18.623597803225053	16.0	8.0	28.0	7.0	35.0
30	18.382901963075486	15.0	8.0	28.0	7.0	35.0
31	18.297499415751343	15.0	8.0	28.0	7.0	35.0
32	17.974599789670485	15.0	8.0	27.0	7.0	35.0
33	17.998320285113344	15.0	8.0	27.0	7.0	35.0
34	17.633544636597335	15.0	8.0	26.0	7.0	34.0
35	17.411690815611124	15.0	8.0	26.0	7.0	34.0
36	17.262648983407338	14.0	8.0	26.0	7.0	34.0
37	17.170396120588922	14.0	8.0	25.0	7.0	33.0
38	17.158842603412012	13.0	8.0	26.0	7.0	33.0
39	16.842530380930125	13.0	8.0	25.0	7.0	33.0
40	16.19806029446132	13.0	8.0	24.0	7.0	33.0
41	16.477930007010983	13.0	8.0	24.0	7.0	33.0
42	16.51611065669549	13.0	8.0	24.0	7.0	33.0
43	16.560557373218042	13.0	8.0	24.0	7.0	33.0
44	16.50417737789203	13.0	8.0	24.0	7.0	33.0
45	16.612540897405935	13.0	8.0	24.0	7.0	33.0
46	16.39699696190699	13.0	8.0	24.0	7.0	33.0
47	16.24915283944847	13.0	8.0	24.0	7.0	32.0
48	16.17228032250526	13.0	8.0	24.0	7.0	32.0
49	15.89070168263613	12.0	8.0	23.0	7.0	32.0
50	15.87530673054452	12.0	8.0	23.0	7.0	32.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-1.6418406216466153
1101	2	-1.757322633209352
1101	3	-1.8382789671585904
1101	4	-2.186021825974482
1101	5	-1.0829415925067067
1101	6	-0.9178337630643902
1101	7	-1.4813750637577208
1101	8	0.5520707059499941
1101	9	-0.9089226435607962
1101	10	0.25046598738014225
1101	11	-0.3859565239109273
1101	12	-0.8181363550308554
1101	13	-0.989208962235768
1101	14	-0.8573983958522078
1101	15	-1.3713368063552558
1101	16	-1.2225369564029087
1101	17	-1.7588254403982475
1101	18	-1.3066077918460692
1101	19	-2.2120637793741444
1101	20	-2.5344961504131405
1101	21	-1.9535754602675013
1101	22	-1.4153983090098556
1101	23	-1.5579043256819212
1101	24	-1.3671425100803454
1101	25	-2.2029100870151197
1101	26	-2.259316537718842
1101	27	-2.0117046505707528
1101	28	-2.959172408946703
1101	29	-2.379506329609953
1101	30	-1.2708412484654872
1101	31	-2.0982919393883535
1101	32	-0.8800277174998463
1101	33	-0.9504498491913473
1101	34	-0.7989747893811874
1101	35	-0.7120078735401023
1101	36	-1.351605356465317
1101	37	-2.073101014070705
1101	38	-1.1207616606810547
1101	39	-0.16807112011614578
1101	40	-0.7329289081882298
1101	41	-0.7683478900120821
1101	42	-1.4020402112350858
1101	43	-0.9410171760816883
1101	44	-1.2349291654088184
1101	45	-1.054815524406978
1101	46	-1.0449050654911822
1101	47	-0.6951273830912879
1101	48	-1.0777839585901656
1101	49	-1.0310764042868712
1101	50	-1.1311023734149739
1102	1	-1.598756721419857
1102	2	-1.6600231073384002
1102	3	-0.5863721438231977
1102	4	-2.4092747635131353
1102	5	-1.4147288528653945
1102	6	-2.5616185497063206
1102	7	-2.4298392979362404
1102	8	-2.67334673375316
1102	9	-1.5764138824806047
1102	10	-2.3862068635784226
1102	11	-1.618382827765803
1102	12	-2.3633847574221143
1102	13	-1.8737688467956524
1102	14	-2.104563928732027
1102	15	-1.2581642646112847
1102	16	-2.4888737941683203
1102	17	-1.7425401383986596
1102	18	-2.727654998607562
1102	19	-2.6322864138824933
1102	20	-2.6297342456512354
1102	21	-1.9513078865713567
1102	22	-2.45776070851511
1102	23	-1.7062242597161408
1102	24	-2.5881278739228506
1102	25	-1.9255445953639168
1102	26	-3.2604503245669143
1102	27	-2.4301750690411694
1102	28	-2.030600980375276
1102	29	-1.5297846227453888
1102	30	-2.881746216513312
1102	31	-3.187861100386085
1102	32	-1.8774509292087735
1102	33	-2.275537459993245
1102	34	-2.66817701572627
1102	35	-2.1737683353005632
1102	36	-1.3526360717817472
1102	37	-1.7452304719144482
1102	38	-2.5275849960758183
1102	39	-1.4876959397409664
1102	40	-0.8405355872234814
1102	41	-1.8647197720982494
1102	42	-2.1166351401157275
1102	43	-1.0472839252055817
1102	44	-2.9149951311890696
1102	45	-2.5605875301789833
1102	46	-3.0718067352499947
1102	47	-2.5360880097662015
1102	48	-2.975227784605419
1102	49	-1.9617092634911586
1102	50	-1.561941375682549
1103	1	-1.1091608830845239
1103	2	-1.0867938156217107
1103	3	-1.6231868697135532
1103	4	-0.5229202216964062
1103	5	-0.7834763518649943
1103	6	-0.16025800548863245
1103	7	-0.6887428593608114
1103	8	-0.7883927521605223
1103	9	-1.61242828824291
1103	10	-1.7379475598569343
1103	11	-0.4709238441723649
1103	12	-0.011243901079577512
1103	13	1.158444038358411
1103	14	-0.0861565657868475
1103	15	-0.2661674658917974
1103	16	-0.47446803186339537
1103	17	-1.16851052655392
1103	18	0.22072435313313576
1103	19	0.20504852009109698
1103	20	-0.45606477786412114
1103	21	-0.6954055256269775
1103	22	-1.038392961416271
1103	23	0.13431195476965385
1103	24	-1.5537141084166493
1103	25	-1.051394935499971
1103	26	-0.7756564069998895
1103	27	-0.5016036404697424
1103	28	-0.968976330515332
1103	29	0.10266835844708666
1103	30	-0.1570563405629315
1103	31	-0.9283572988654782
1103	32	-0.6423568915937281
1103	33	-1.8565698729584312
1103	34	-0.8328428820728071
1103	35	-1.3810512484658304
1103	36	-1.2015756476120778
1103	37	0.15833095265537978
1103	38	-1.2814865567000684
1103	39	-0.42327016943284335
1103	40	-0.5306116176356461
1103	41	-1.2062563867441085
1103	42	-0.18546267112813197
1103	43	-2.00726791880302
1103	44	-1.627680205218681
1103	45	-2.1852373901229605
1103	46	-0.46736495853931004
1103	47	-0.44765263561654045
1103	48	-0.33417136203639153
1103	49	0.023884974203919995
1103	50	0.40484533900332487
1104	1	1.189991210185216
1104	2	1.247864371977652
1104	3	0.8532307243510999
1104	4	0.5756489356962788
1104	5	0.9457234361583211
1104	6	0.6055077602771313
1104	7	0.9636153812327244
1104	8	1.4537906076698945
1104	9	0.2799935453553921
1104	10	0.5813423182564748
1104	11	1.9053347673803636
1104	12	0.8234142865197889
1104	13	1.2562182831914797
1104	14	2.5630220245682125
1104	15	1.4746910396725923
1104	16	1.6352299013639495
1104	17	0.5190888375160299
1104	18	0.6046671194288429
1104	19	0.22200665469629044
1104	20	-0.029991645908637565
1104	21	0.5989770922850504
1104	22	1.3757564821449364
1104	23	1.4459176781400842
1104	24	2.064197921260085
1104	25	0.8945510104459764
1104	26	-0.3914486698509734
1104	27	0.5569549180888167
1104	28	-0.43664988642418123
1104	29	-0.535116485220108
1104	30	-0.6093615869858269
1104	31	0.6884948473984345
1104	32	0.9935731561010286
1104	33	0.9621900634485634
1104	34	1.0134740230676265
1104	35	0.3783552168229889
1104	36	-0.4217664266263874
1104	37	0.11090116993148058
1104	38	-0.19802073794013353
1104	39	0.7587648067197783
1104	40	1.3710841958248743
1104	41	0.6670875454233531
1104	42	-1.3266921358870096
1104	43	0.07672784166332747
1104	44	1.4115354810558287
1104	45	0.9612914917000381
1104	46	0.4383054177193024
1104	47	-0.3080129959769007
1104	48	0.7455848647786567
1104	49	-0.2975246707351378
1104	50	0.3884171461045458
1105	1	-1.0082936130996067
1105	2	-0.6484453993321182
1105	3	-1.3520707309503557
1105	4	-1.5668493318019863
1105	5	-2.038943923509038
1105	6	-1.192309287539917
1105	7	-1.474187806570466
1105	8	-2.7993278954486094
1105	9	-3.100550460188611
1105	10	-2.9229023109881567
1105	11	-2.5793325922869954
1105	12	-2.4318698437643427
1105	13	-2.735032583059386
1105	14	-1.9140223274761397
1105	15	-1.491092051110499
1105	16	-3.0830653169312683
1105	17	-2.5567081132809193
1105	18	-2.6689815292198062
1105	19	-1.655245597555961
1105	20	-2.0569320478490383
1105	21	-1.6277207594127994
1105	22	-1.5427293113408567
1105	23	-1.7629354057130016
1105	24	-1.2682691612069945
1105	25	-1.5289590380640732
1105	26	-2.0712823496846546
1105	27	-2.1746805635466657
1105	28	-2.103403178177473
1105	29	-2.738772063875688
1105	30	-2.6136928413170804
1105	31	-1.4166680077644216
1105	32	-1.9306298931020223
1105	33	-1.4284024521439491
1105	34	-2.8094642998706956
1105	35	-1.6037133902456198
1105	36	-1.558287563147525
1105	37	-1.3582602992299897
1105	38	-1.3770747919941861
1105	39	-0.7679157199607474
1105	40	-1.475935901195223
1105	41	-1.5642880609522525
1105	42	-1.0063525655474397
1105	43	-1.5268757619402749
1105	44	-2.184618365098018
1105	45	-1.8256974202888738
1105	46	-0.8604645060506222
1105	47	-1.4059859689498744
1105	48	-1.782135162941369
1105	49	-1.1559792791897454
1105	50	-2.0242647665773674
1106	1	-1.4450152248212191
1106	2	-0.6260095018962204
1106	3	-0.15371908259870892
1106	4	0.02609938614672913
1106	5	-0.04398055354566566
1106	6	-1.9412103864410142
1106	7	-0.8652134475961049
1106	8	-0.9379725840932984
1106	9	-0.6947812294193803
1106	10	-1.3079755710614158
1106	11	-1.5224644604188633
1106	12	-1.0028405397350397
1106	13	-1.7309117039385065
1106	14	-1.3685095069633206
1106	15	-1.9670078020262523
1106	16	-0.502479236345188
1106	17	-0.011927893500701003
1106	18	-1.5582672435055223
1106	19	-0.6969122642226289
1106	20	-1.3059247218417127
1106	21	-2.2519881586801986
1106	22	-1.9495974432089902
1106	23	-1.7177888855664811
1106	24	-1.3296244725623083
1106	25	-1.4894901735952075
1106	26	-0.967253045655351
1106	27	-1.8301750690411716
1106	28	-1.6067914565657517
1106	29	-1.6522336023372262
1106	30	-1.135487713111953
1106	31	-1.3300379711343844
1106	32	-1.1039815414536704
1106	33	-0.5394830382245352
1106	34	-0.5123947028010996
1106	35	-0.8785302400624673
1106	36	-1.2601190649790261
1106	37	-1.9758427168124086
1106	38	-1.7092176491370434
1106	39	-1.2829340349790623
1106	40	-1.5398553151146377
1106	41	-1.4667605884247799
1106	42	-1.714594323789198
1106	43	-0.04116147622599087
1106	44	0.37344024296059075
1106	45	-0.917730387321841
1106	46	-0.73235095293707
1106	47	-0.7571764451403507
1106	48	-1.5187651995714067
1106	49	-1.7528657260761928
1106	50	-1.3469753892879908
1107	1	-0.7276992075052
1107	2	-0.9472216231083443
1107	3	-1.0887840176636416
1107	4	-0.034506674459329645
1107	5	-0.6950628046279199
1107	6	-0.7451064903371183
1107	7	-1.5227892051718648
1107	8	-1.284292930413642
1107	9	-1.167508502146653
1107	10	-0.6010491641350093
1107	11	-0.41726965522405735
1107	12	-1.4747020115965093
1107	13	-1.0387039117307175
1107	14	-1.0897216281754396
1107	15	-1.3622458972643443
1107	16	-1.6942541281200825
1107	17	-1.2820577636305686
1107	18	-1.3530724383107149
1107	19	-1.0484274157377804
1107	20	-0.7466173625343551
1107	21	-1.9121613188533608
1107	22	-1.99115588476743
1107	23	-2.2861871539647467
1107	24	-0.7287586716965073
1107	25	0.16981718571215154
1107	26	-0.7118417902440939
1107	27	0.15294181407571372
1107	28	-0.613717863492159
1107	29	-2.0067790568826815
1107	30	-1.6385180161422568
1107	31	-0.7477868888833008
1107	32	-2.1870984245705536
1107	33	-0.3464094451509432
1107	34	-0.28079297119936797
1107	35	-0.4594826210148497
1107	36	0.9584956536356941
1107	37	-0.3044141453838378
1107	38	-0.2975293374487329
1107	39	-0.7721115241565499
1107	40	-1.178383453642775
1107	41	-0.5057216273858192
1107	42	-1.2899189991138726
1107	43	-0.7814212164857288
1107	44	-1.852100882580535
1107	45	-2.243704413295866
1107	46	-0.8085414291275459
1107	47	-0.32644051440441935
1107	48	-1.1282890090952158
1107	49	-1.483601656812123
1107	50	-1.4997892421018442
1108	1	0.19734066490309488
1108	2	-1.047062133475169
1108	3	-0.19181432069394688
1108	4	0.4997835966730477
1108	5	0.10764851913603835
1108	6	0.8485139243359292
1108	7	0.5119795348600356
1108	8	-0.05877458910582689
1108	9	0.022762630229742342
1108	10	-0.32050689938221666
1108	11	0.7690142363229917
1108	12	-0.40133678033654263
1108	13	-0.5040946613319903
1108	14	-0.43342178766507544
1108	15	0.7131926992268838
1108	16	0.010052091975612854
1108	17	0.7592500513614553
1108	18	-0.34222714325489534
1108	19	-0.42498243966122473
1108	20	-0.3941452732201576
1108	21	-0.07930645441954809
1108	22	-0.6673919294245287
1108	23	0.03008078861898511
1108	24	-0.6053136956199516
1108	25	1.1064998013421352
1108	26	0.07986474883086458
1108	27	1.3405016226881514
1108	28	1.2776696963164547
1108	29	0.4091699064347054
1108	30	0.5101263219757683
1108	31	1.5391349611964422
1108	32	-0.05360560160404759
1108	33	0.3379605707980211
1108	34	-0.3013671338787951
1108	35	0.2422717649500612
1108	36	0.1178257971763621
1108	37	-0.47634396994524053
1108	38	-0.37488180953804573
1108	39	0.628845413642491
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2210	37	1.7862400602236406
2210	38	1.2152149276319832
2210	39	0.11777802554608385
2210	40	2.083553674649494
2210	41	1.6387133768622704
2210	42	1.6656459966380375
2210	43	1.6226569483400972
2210	44	0.4769560418034935
2210	45	0.7912148730252895
2210	46	0.10547726246123901
2210	47	0.7937803861903134
2210	48	1.4885334208113257
2210	49	1.619541928574451
2210	50	2.1098114375922936
2211	1	-0.7238759279809877
2211	2	-0.7506200937965346
2211	3	-0.45972709327961425
2211	4	-0.7838694612052954
2211	5	-0.5490984885701451
2211	6	-0.7615041114076355
2211	7	-0.14870254416931417
2211	8	-0.28225384800072817
2211	9	-0.8966503882978856
2211	10	-0.8135385439141096
2211	11	-0.09416005454436416
2211	12	0.3732164251069747
2211	13	0.7724260797731084
2211	14	-1.06508271568606
2211	15	-0.9014982337129425
2211	16	-0.7476950796918729
2211	17	0.43960748700664354
2211	18	-1.3991217161356957
2211	19	-0.4508063452195117
2211	20	0.9109867156304716
2211	21	0.18868829881868976
2211	22	-0.35734110141993725
2211	23	-0.5926442482723111
2211	24	1.1025072586348443
2211	25	0.017140890045201473
2211	26	0.543383358438998
2211	27	0.605872995044276
2211	28	0.4240051611467557
2211	29	0.11412153784969092
2211	30	-0.18720110875058182
2211	31	0.01593443652026494
2211	32	0.960904973900135
2211	33	0.13243507481507066
2211	34	1.0931237662687714
2211	35	0.7741622387982332
2211	36	1.5157598847317004
2211	37	1.3563335181675669
2211	38	1.3273644603422632
2211	39	1.005628492835804
2211	40	0.16766582418220466
2211	41	1.405068517049184
2211	42	1.2170478657969177
2211	43	0.8563018081531837
2211	44	0.7292924904016225
2211	45	1.6510279571374369
2211	46	1.7409912811528265
2211	47	1.7470514142276947
2211	48	2.180122205858055
2211	49	1.7503830500697806
2211	50	0.446260035723137
2212	1	0.9283899863198428
2212	2	0.27336156728616956
2212	3	0.21070140257649328
2212	4	0.23050190186999941
2212	5	1.2246627528334884
2212	6	0.7296791014296069
2212	7	0.9964217725296791
2212	8	0.9448665893119141
2212	9	0.9391810347315612
2212	10	0.5594997209421777
2212	11	-1.2025183687746583
2212	12	-0.24228348672515665
2212	13	0.008980479349901316
2212	14	-0.6917799472148936
2212	15	0.30190504613188196
2212	16	-0.5743570440720553
2212	17	-0.5340303193767113
2212	18	-0.09115134054055929
2212	19	-0.8510003145370924
2212	20	-0.380408100098677
2212	21	-0.6663637202255721
2212	22	-0.858222780136213
2212	23	-0.8244375827812149
2212	24	-0.28604855162789633
2212	25	-1.6771810990219826
2212	26	0.46500212056207957
2212	27	-0.643113074432005
2212	28	-0.2893610881919848
2212	29	-0.5861958664881683
2212	30	-0.9587581533635259
2212	31	-0.42428774651623513
2212	32	0.3768809922390517
2212	33	0.36096619807375774
2212	34	-0.786967928317722
2212	35	-1.2896713002601317
2212	36	-0.43927450073823593
2212	37	-0.5817726359498749
2212	38	-0.450187999541356
2212	39	-1.844838796883824
2212	40	-2.093134740092175
2212	41	-1.2389977851902785
2212	42	-1.3528692564037534
2212	43	-0.828762554393105
2212	44	-0.918653198189455
2212	45	0.5863127393627963
2212	46	-0.12552256120301664
2212	47	-0.7432501198932702
2212	48	-1.4490437260763471
2212	49	-0.6875467683044043
2212	50	-0.19189901911728136
2213	1	0.5359371561311619
2213	2	1.5436874678007477
2213	3	1.2657614368818102
2213	4	1.2564024164497596
2213	5	0.7049371953720822
2213	6	1.609438964208337
2213	7	1.695392613009954
2213	8	2.6884343423136308
2213	9	2.1234005887624363
2213	10	3.1534962904104447
2213	11	1.3463667084123045
2213	12	1.298025261130764
2213	13	1.4885688155420098
2213	14	0.9102783718245604
2213	15	2.001390466372019
2213	16	2.5421095082435556
2213	17	1.945214963642158
2213	18	1.492382107143829
2213	19	2.1606634933531303
2213	20	1.2988371829201917
2213	21	0.79692959023755
2213	22	1.0724804788689326
2213	23	1.404721936944341
2213	24	1.125786782848948
2213	25	1.0789080948030616
2213	26	1.5972491188468148
2213	27	0.4983963595302576
2213	28	-0.08644513621943162
2213	29	1.0659482158445925
2213	30	0.5069365293122914
2213	31	1.5794858383894237
2213	32	2.5674470299749004
2213	33	0.5899541912126942
2213	34	0.9192070288006313
2213	35	0.2768810153487884
2213	36	1.2403138354538754
2213	37	-0.23168687265656374
2213	38	1.5933797534603578
2213	39	0.81879756675254
2213	40	1.6579801827208591
2213	41	0.44882382715963587
2213	42	1.6373537281588533
2213	43	1.3822151471506352
2213	44	1.1569900265103747
2213	45	1.1835683139768598
2213	46	0.5096403890542724
2213	47	0.8553776674137623
2213	48	0.026256445450240307
2213	49	0.08003470682423952
2213	50	0.4820289397163382
2214	1	1.4782794984735048
2214	2	1.3289454530587328
2214	3	2.060437931558308
2214	4	1.4765498365971794
2214	5	0.4412189316538182
2214	6	2.776678931448302
2214	7	1.5852370028543454
2214	8	1.399736553615842
2214	9	2.0277412931031407
2214	10	1.2660270029411578
2214	11	0.3377671998127987
2214	12	0.3189097820152824
2214	13	0.9318939588671533
2214	14	0.31977878132496684
2214	15	0.5287450937266449
2214	16	0.824419090553139
2214	17	0.1317014501286451
2214	18	1.5506130653747867
2214	19	0.8436282763179115
2214	20	0.4294678135508221
2214	21	0.7791572724652305
2214	22	0.19557630196475628
2214	23	1.617088849311255
2214	24	1.0822159392781039
2214	25	1.1468032626982279
2214	26	1.329272050869747
2214	27	1.6200179811518787
2214	28	0.9507375009632071
2214	29	1.1405591904555656
2214	30	1.6969447193204807
2214	31	1.3911975501011362
2214	32	0.9215010840289537
2214	33	0.9531810544395576
2214	34	0.9594199690135738
2214	35	0.9279047663725386
2214	36	1.5512065463465845
2214	37	1.5433336023639121
2214	38	1.081258541339146
2214	39	0.9389449868999584
2214	40	0.9026157273564053
2214	41	1.7121325904684
2214	42	1.1237583145634424
2214	43	1.4280791930146783
2214	44	1.6727967423170895
2214	45	1.5288590592676066
2214	46	0.9968639762778615
2214	47	0.881176193212287
2214	48	2.1690082882020825
2214	49	0.8826555094450423
2214	50	1.0731018307892288
2215	1	-0.16213976694576004
2215	2	0.9765546006678818
2215	3	0.23446773058810422
2215	4	-0.29761856257121977
2215	5	-1.2216362312013445
2215	6	0.3653830201523931
2215	7	0.22773527035261054
2215	8	-0.2704817416024561
2215	9	0.8032956936575424
2215	10	-0.4325176956035399
2215	11	-0.4254864384408421
2215	12	-0.2972544591489594
2215	13	-0.03310950613630936
2215	14	1.3840545956007837
2215	15	0.403138718120271
2215	16	0.48424237537642156
2215	17	1.5875226559498508
2215	18	0.590633855395577
2215	19	1.2774467101363456
2215	20	0.41422179830480843
2215	21	0.9780484713564306
2215	22	0.8322706886591433
2215	23	1.5255261327485385
2215	24	1.750961608023772
2215	25	0.2595025003974669
2215	26	0.3229484195461154
2215	27	-0.6266036404697424
2215	28	0.3389045141302205
2215	29	0.7227663976627738
2215	30	1.7036148509906113
2215	31	0.4294858383894251
2215	32	0.7328316453595178
2215	33	0.7542898555483575
2215	34	1.3059203155139194
2215	35	1.1655173789851503
2215	36	0.28786628300632344
2215	37	0.9494320084623169
2215	38	-1.1847670996864927
2215	39	-1.489069566114594
2215	40	-1.2451666704259932
2215	41	-0.1989034455676375
2215	42	-0.6025064117012846
2215	43	-0.8730296080941216
2215	44	-0.35769528817494134
2215	45	-0.2487743433657954
2215	46	-1.4470029675890839
2215	47	-0.8194475074114127
2215	48	0.6313263755201675
2215	49	-0.030979279189745412
2215	50	0.42765831034570745
2216	1	1.672019630357969
2216	2	0.32588053246804094
2216	3	0.42330595422014383
2216	4	0.6721474961123661
2216	5	0.9167460051190339
2216	6	0.9668897608343912
2216	7	0.11760442181970276
2216	8	-0.04774185262545316
2216	9	0.3299610386218568
2216	10	0.34543630915458934
2216	11	1.6392439342792215
2216	12	1.915176151477528
2216	13	0.5739483844267355
2216	14	1.251421764514344
2216	15	1.2387850305707069
2216	16	1.2289886085247161
2216	17	1.1400603244668979
2216	18	0.40962671820287255
2216	19	1.0522286292481624
2216	20	0.6648165643634911
2216	21	0.03038788452058583
2216	22	1.1691065332269446
2216	23	0.4413667354448094
2216	24	0.3306602598873738
2216	25	1.161663483744018
2216	26	1.5528255012273213
2216	27	0.369530380148813
2216	28	0.9686626425996891
2216	29	2.038488047147311
2216	30	0.5359899501182888
2216	31	1.4011353229255086
2216	32	1.382910947500676
2216	33	0.7638061124872948
2216	34	0.9292351450143173
2216	35	1.4580431521810269
2216	36	2.4401263940293205
2216	37	0.9708435865749259
2216	38	0.6654509812016904
2216	39	1.0623776979615371
2216	40	1.8022163589157998
2216	41	1.5111481008241157
2216	42	1.3705308603050597
2216	43	1.153536609193747
2216	44	1.5661746563135601
2216	45	1.8201193917650507
2216	46	2.168778158501322
2216	47	0.9973645465140422
2216	48	1.1706800630697316
2216	49	0.9647582307230227
2216	50	0.2251404673639481
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1.0
3	1.0
4	1.0
5	3.0
6	22.0
7	400.0
8	2381.0
9	5539.0
10	4944.0
11	3714.0
12	3718.0
13	3683.0
14	3672.0
15	3571.0
16	3372.0
17	3042.0
18	2738.0
19	2467.0
20	2254.0
21	2123.0
22	2007.0
23	1849.0
24	1724.0
25	1646.0
26	1477.0
27	1395.0
28	1348.0
29	1258.0
30	1184.0
31	1134.0
32	1074.0
33	910.0
34	923.0
35	778.0
36	683.0
37	581.0
38	405.0
39	442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.6196165601556	19.5814626870037	22.00318802006405	27.795732732776653
2	18.60757274745822	28.123174009582797	33.956117798293796	19.313135444665186
3	18.354439624322627	33.381534551509574	27.89828082321838	20.365745000949417
4	17.61827556490367	26.291573550677004	29.744533543665924	26.345617340753403
5	17.66793742605495	39.262083169011	27.813563530666197	15.256415874267853
6	25.0540430007011	39.60767702734284	13.626139284879645	21.71214068707642
7	30.448563457634048	31.710558988066545	14.783167550355666	23.057710003943736
8	24.199932810423146	37.47863809649007	17.67231935498006	20.649109738106713
9	25.15811460204782	27.080320757197317	13.601507383550238	34.16005725720462
10	23.221641202418862	30.43586223014227	25.70330986532675	20.63918670211212
11	32.562589465688994	25.57185007741521	20.259413981478776	21.60614647541702
12	24.48803715930005	26.956559843416787	22.834565160234874	25.72083783704829
13	28.38299737072743	21.29126497224657	22.78702892199825	27.538708735027758
14	34.913377545356276	22.648923427502996	17.72035408571679	24.71734494142394
15	27.025329399047592	26.807677700195743	18.33094744222735	27.83604545852932
16	31.07271902748724	22.279436504337397	20.120247516786378	26.527596951388993
17	38.6050248320187	22.08150744960561	16.113058720420685	23.20040899795501
18	31.86830866436553	19.781489498625167	17.925466565260635	30.42473527174867
19	30.480328900203368	20.50944417621326	21.434110228386665	27.576116695196713
20	36.102531071130564	20.507678119190174	19.83428730365534	23.55550350602392
21	28.36815060972932	23.00575326823698	17.599437848599745	31.026658273433956
22	32.403875663115436	19.14302833676765	16.27720052026247	32.17589547985445
23	39.93133674214756	21.447772096420746	16.33016800584368	22.290723155588022
24	29.456412056333086	29.50321004986911	15.772386258939147	25.267991634858657
25	35.7704448471386	22.4454901502309	15.56234290056702	26.22172210206348
26	34.521252502228265	27.406887885562327	15.640205146188576	22.431654466020838
27	30.734501249251178	27.88094855422919	18.410017387238646	22.974532809280987
28	36.07872359077759	24.961281084714066	17.27448058209871	21.68551474240963
29	32.27837928666404	24.139744882303948	19.95791873054837	23.623957100483644
30	35.6456917342688	22.692392019294015	18.700577358766353	22.96133888767083
31	33.35379040271202	22.022035186159332	21.278274592319832	23.345899818808814
32	30.49475437622374	23.982290540343083	20.96349981004705	24.55945527338613
33	35.61679866732911	21.433790221235057	21.182453166554637	21.7669579448812
34	31.12523746894637	23.03521847143066	22.590968873301183	23.248575186321787
35	33.38837178719114	25.378084954045327	19.37256162602101	21.860981632742522
36	36.359119735803844	21.211678405470966	21.88824268638396	20.540959172341232
37	31.706141055799886	22.033576365044343	23.478616618693472	22.7816659604623
38	33.614378607437715	19.818806166435305	24.303353547161542	22.26346167896544
39	32.37077847111605	20.67252188399655	25.76393049730378	21.192769147583626
40	26.163479685739784	34.03022633172887	22.13866658863806	17.667627393893287
41	32.308029517059985	17.175421933221305	25.618470081098856	24.898078468619858
42	25.26849511229306	21.01348685652498	31.469818957581424	22.24819907360054
43	26.039627107747158	17.310558461673338	29.305356673193256	27.344457757386248
44	26.40191695157944	16.95157943952544	32.22874842932702	24.4177551795681
45	25.960667426434057	18.064930890388943	34.098945092194846	21.875456590982147
46	25.26661796932067	20.509861212563916	28.80788897005113	25.41563184806428
47	30.536481313736374	19.917891476492418	29.37059872015896	20.17502848961225
48	30.528730258448146	22.781129925343695	24.730083130013004	21.96005668619516
49	28.54283543712015	23.153342683496962	25.60191678354371	22.701905095839177
50	30.367891999298696	23.796096078545965	22.476769329670972	23.359242592484367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	2.0
6	1.0
7	1.5
8	2.0
9	4.0
10	6.0
11	11.0
12	16.0
13	15.5
14	15.0
15	19.5
16	24.0
17	36.0
18	48.0
19	66.0
20	84.0
21	103.5
22	123.0
23	175.5
24	228.0
25	262.5
26	297.0
27	385.5
28	474.0
29	566.0
30	658.0
31	793.0
32	928.0
33	1109.0
34	1290.0
35	1470.5
36	1651.0
37	1845.5
38	2040.0
39	2217.0
40	2394.0
41	2638.0
42	2882.0
43	3070.5
44	3259.0
45	3431.5
46	3604.0
47	3832.0
48	4060.0
49	4410.0
50	4760.0
51	4866.5
52	4973.0
53	4956.5
54	4940.0
55	4764.5
56	4589.0
57	4384.5
58	4180.0
59	3807.0
60	3434.0
61	3301.0
62	3168.0
63	2849.5
64	2531.0
65	2371.5
66	2212.0
67	2059.5
68	1907.0
69	1765.5
70	1624.0
71	1496.5
72	1369.0
73	1272.5
74	1176.0
75	1095.0
76	1014.0
77	904.0
78	794.0
79	696.0
80	598.0
81	507.0
82	416.0
83	341.0
84	266.0
85	217.5
86	169.0
87	142.0
88	115.0
89	92.5
90	70.0
91	49.5
92	29.0
93	25.0
94	21.0
95	15.0
96	9.0
97	7.0
98	5.0
99	6.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12123159616732881
2	0.011684973124561814
3	0.0014606216405702267
4	0.0014606216405702267
5	0.0014606216405702267
6	0.0
7	0.0014606216405702267
8	0.0014606216405702267
9	0.0014606216405702267
10	0.0029212432811404534
11	0.0029212432811404534
12	0.0029212432811404534
13	0.005842486562280907
14	0.00876372984342136
15	0.00876372984342136
16	0.15336527225987379
17	0.005842486562280907
18	0.13437719093246087
19	0.16797148866557607
20	0.22347511100724468
21	0.22639635428838514
22	0.05404300070109839
23	0.020448702967983175
24	0.12415283944846928
25	0.05258237906052816
26	0.036515541014255665
27	0.033594297733115216
28	0.032133676092544985
29	0.036515541014255665
30	0.0715704603879411
31	0.04089740593596635
32	0.037976162654825896
33	0.043818649217106806
34	0.04966113577938771
35	0.03943678429539612
36	0.043818649217106806
37	0.033594297733115216
38	0.042358027576536575
39	0.05112175741995794
40	0.1650502453844356
41	0.042358027576536575
42	0.03943678429539612
43	0.037976162654825896
44	0.032133676092544985
45	0.032133676092544985
46	0.020448702967983175
47	0.02629118953026408
48	0.024830567889693855
49	0.023369946249123627
50	0.02921243281140453
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	68464.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.69753447067072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.83388970288355	95.5699345641505
2	0.7205111550836064	1.3934330451039962
3	0.17823965681313536	0.5170600607618602
4	0.058909717082307446	0.22785697592895537
5	0.060420222648520455	0.2921243281140453
6	0.028699605758047215	0.16651086702500584
7	0.01963657236076915	0.13291656929189063
8	0.025678594625621193	0.19864454311755084
9	0.00906303339727807	0.07887356859079224
>10	0.058909717082307446	0.9976045805094649
>50	0.006042022264852046	0.425040897405936
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG	95	0.13875905585417153	No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC	74	0.10808600140219678	No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG	69	0.10078289319934565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00876372984342136	0.0
2	0.0	0.0	0.0	0.00876372984342136	0.0
3	0.0	0.0	0.0	0.00876372984342136	0.0
4	0.0	0.0	0.0	0.00876372984342136	0.0
5	0.0	0.0	0.0	0.00876372984342136	0.0
6	0.0	0.0	0.0	0.00876372984342136	0.0
7	0.0	0.0	0.0	0.010224351483991586	0.0
8	0.0	0.0	0.0	0.011684973124561814	0.0
9	0.0	0.0	0.0	0.011684973124561814	0.0
10	0.0	0.0	0.0	0.014606216405702267	0.0
11	0.0	0.0	0.0	0.018988081327412948	0.0
12	0.0	0.0	0.0	0.018988081327412948	0.0
13	0.0	0.0	0.0	0.018988081327412948	0.0
14	0.0	0.0	0.0	0.018988081327412948	0.0
15	0.0	0.0	0.0	0.018988081327412948	0.0
16	0.0	0.0	0.0	0.018988081327412948	0.0
17	0.0	0.0	0.0	0.0219093246085534	0.0
18	0.0	0.0	0.0	0.032133676092544985	0.0
19	0.0	0.0	0.0	0.08179481187193269	0.0
20	0.0	0.0	0.0	0.09347978499649451	0.0
21	0.0	0.0	0.0	0.09347978499649451	0.0
22	0.0	0.0	0.0	0.11100724468333723	0.0
23	0.0	0.0	0.0	0.11246786632390746	0.0
24	0.0	0.0	0.0	0.11246786632390746	0.0
25	0.0	0.0	0.0	0.11392848796447769	0.0
26	0.0	0.0	0.0	0.1314559476513204	0.0
27	0.0	0.0	0.0	0.14460154241645246	0.0
28	0.0	0.0	0.0	0.14898340733816312	0.0
29	0.0	0.0	0.0	0.16797148866557607	0.0
30	0.0	0.0	0.0	0.17089273194671653	0.0
31	0.0	0.0	0.0	0.20156578639869127	0.0
32	0.0	0.0	0.0	0.21179013788268286	0.0
33	0.0	0.0	0.0	0.23662070577237673	0.0
34	0.0	0.0	0.0	0.31403365272259876	0.0
35	0.0	0.0	0.0	0.3885253563916803	0.0
36	0.0	0.0	0.0	0.4820051413881748	0.0
37	0.0	0.0	0.0	0.6382916569291891	0.0
38	0.0	0.0	0.0	0.7901963075484927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	20	7.818028E-4	43.98465	30
ATGACGC	20	7.846253E-4	43.95252	9
GTTATAC	30	1.2909895E-4	36.653873	1
TGATCGT	25	0.0023413626	35.18772	31
TAAAGAT	25	0.0023413626	35.18772	1
AATGATC	25	0.0023413626	35.18772	29
GGTGGTC	75	1.8189894E-12	35.18772	44
TATAACA	25	0.002349783	35.162018	3
CGGTGGT	95	0.0	32.409744	43
TTTCGGT	35	3.1929996E-4	31.417608	43
CACATAT	35	3.2067363E-4	31.394659	4
TTTACAC	85	7.2759576E-12	31.025307	2
CCGGTGC	30	0.0056766374	29.366032	15
TGTATAT	30	0.005697005	29.34455	37
CGAGAAT	30	0.005697005	29.34455	25
TTCGGTG	45	4.0239152E-5	29.323101	44
CTTATAC	480	0.0	28.406752	1
TCGTGTA	40	6.94848E-4	27.510515	34
TAATAAG	40	6.9782976E-4	27.490406	1
TTATAAC	40	7.0082204E-4	27.470324	2
>>END_MODULE