FastQCFastQC Report
Wed 25 May 2016
SRR1780538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51923
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG150.002213887669.99229464
TAGAGCT150.002213887669.9922944
TTCTATC252.3710438E-455.99383530
ATCGCCT252.3710438E-455.99383534
TCTATCG252.3710438E-455.99383531
CGGGTGA200.006917893452.4942255
GACCCCG200.006917893452.494227
GCGAGGA200.006917893452.4942223
CCGTGGG305.830545E-446.661539
TCTAGAA305.830545E-446.661532
TAAATGG305.830545E-446.6615355
TATCGCC305.830545E-446.6615333
AATTTCT350.001245398739.99559427
TACTTTA350.001245398739.99559442
CTATCGC350.001245398739.99559432
GGGTAAA350.001245398739.99559452
CCTACTA350.001245398739.9955944
CTAAGGT350.001245398739.99559467
TAGCCCC350.001245398739.99559453
TATTGCG350.001245398739.99559411
Produced by FastQC (version 0.11.5)