##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780538_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51923 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.989657762455945 34.0 32.0 34.0 21.0 34.0 2 32.40172948404368 34.0 34.0 34.0 31.0 34.0 3 32.5300348593109 34.0 34.0 34.0 31.0 34.0 4 32.707066232690714 34.0 34.0 34.0 32.0 34.0 5 32.80654045413401 34.0 34.0 34.0 32.0 34.0 6 35.59235791460432 38.0 37.0 38.0 31.0 38.0 7 35.188683242493696 38.0 36.0 38.0 27.0 38.0 8 34.258594457176976 38.0 34.0 38.0 24.0 38.0 9 34.924927296188585 38.0 36.0 38.0 27.0 38.0 10-11 35.35247385551682 38.0 36.5 38.0 29.0 38.0 12-13 35.587687537314864 38.0 37.0 38.0 31.0 38.0 14-15 35.307021936328795 38.0 36.0 38.0 29.0 38.0 16-17 35.17339136798721 38.0 36.0 38.0 27.0 38.0 18-19 35.159775051518594 38.0 36.0 38.0 29.0 38.0 20-21 35.21335439015465 38.0 36.0 38.0 27.0 38.0 22-23 35.17441211024017 38.0 36.0 38.0 29.0 38.0 24-25 35.596566068986775 38.0 37.0 38.0 31.0 38.0 26-27 35.58396086512721 38.0 37.0 38.0 31.0 38.0 28-29 35.81509157791345 38.0 37.0 38.0 32.0 38.0 30-31 35.735993682953605 38.0 37.0 38.0 31.0 38.0 32-33 35.26625965371801 38.0 36.0 38.0 29.0 38.0 34-35 35.37478574042332 38.0 36.0 38.0 31.0 38.0 36-37 35.40728578857154 38.0 36.5 38.0 29.0 38.0 38-39 35.83345530882268 38.0 37.0 38.0 32.0 38.0 40-41 35.39186872869441 38.0 36.5 38.0 30.0 38.0 42-43 35.66388690946209 38.0 37.0 38.0 31.0 38.0 44-45 34.72199218072916 38.0 35.0 38.0 26.0 38.0 46-47 35.4642643915028 38.0 36.5 38.0 31.0 38.0 48-49 35.27112262388537 38.0 36.0 38.0 29.0 38.0 50-51 35.05900082814937 38.0 36.0 38.0 29.0 38.0 52-53 35.0812549351925 38.0 36.0 38.0 27.0 38.0 54-55 34.826165668393585 37.5 35.5 38.0 27.0 38.0 56-57 34.91962136240202 37.5 35.5 38.0 28.0 38.0 58-59 34.87114573503072 37.5 35.5 38.0 28.0 38.0 60-61 34.854120524623 37.5 35.5 38.0 26.5 38.0 62-63 34.87518055582305 37.5 36.0 38.0 27.0 38.0 64-65 35.01645706141787 38.0 36.0 38.0 27.0 38.0 66-67 34.9506191861025 38.0 36.0 38.0 27.0 38.0 68-69 34.3450108814976 37.0 34.5 38.0 25.5 38.0 70-71 34.46037401536891 37.0 34.5 38.0 25.0 38.0 72-73 34.58887198351405 37.0 35.0 38.0 26.5 38.0 74-75 34.071943069545284 37.0 34.0 38.0 24.5 38.0 76 33.430868786472274 37.0 32.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.8655875742439676 1101 2 -0.5592822702925133 1101 3 0.24422645662743037 1101 4 -2.331511495694226 1101 5 -0.3153517604259761 1101 6 -1.7301667554369615 1101 7 -1.257428416202103 1101 8 -0.7882544127216775 1101 9 -1.0632665937461994 1101 10-11 -1.3477992407328756 1101 12-13 -1.3148403276743394 1101 14-15 -0.3560560132124877 1101 16-17 -2.3095538044727917 1101 18-19 0.018399761796224823 1101 20-21 -1.3236159314753309 1101 22-23 -0.6707175219769539 1101 24-25 -0.7824928339662947 1101 26-27 -1.748191252619634 1101 28-29 -0.7775949159849844 1101 30-31 -0.9254632437566883 1101 32-33 0.22138892403626187 1101 34-35 -1.799480178884032 1101 36-37 0.07269952670171165 1101 38-39 -1.180455528522593 1101 40-41 -0.7641309999174126 1101 42-43 -0.7819727815370925 1101 44-45 0.21328873648509727 1101 46-47 0.22228186491071256 1101 48-49 -1.765452404425453 1101 50-51 -1.7938015950078352 1101 52-53 -0.5410625479436888 1101 54-55 -1.3527522227887872 1101 56-57 -0.956694025292883 1101 58-59 -0.734126271023932 1101 60-61 -0.4785785932577866 1101 62-63 -2.2340833274316907 1101 64-65 -0.8489835431545387 1101 66-67 -1.466580675553324 1101 68-69 -0.17301133935593072 1101 70-71 -1.3992978588763876 1101 72-73 -1.3802187517398394 1101 74-75 -1.583739116445912 1101 76 -1.0100097512080168 1102 1 0.11930253564614546 1102 2 -0.562945273955517 1102 3 -0.15870394630297113 1102 4 0.06684015265742005 1102 5 0.23638816631394377 1102 6 0.03631676104654957 1102 7 1.0282858695121817 1102 8 1.942056942589673 1102 9 1.1697920143124065 1102 10-11 -0.3384127938464232 1102 12-13 -4.0105820859160985 1102 14-15 -1.441678723835203 1102 16-17 -2.056577613996602 1102 18-19 -1.3751441942477314 1102 20-21 0.008801650942245942 1102 22-23 -1.0528145915740268 1102 24-25 0.32831302683955954 1102 26-27 -5.675389054817444 1102 28-29 -0.4410564544465245 1102 30-31 0.5092895034960563 1102 32-33 -7.151321698674366 1102 34-35 -0.9437109481148056 1102 36-37 -0.18714479564260955 1102 38-39 0.33076242019534874 1102 40-41 0.5559239451375362 1102 42-43 0.4130821635178492 1102 44-45 -6.934376098679735 1102 46-47 -0.49200384937499564 1102 48-49 0.004007302534255075 1102 50-51 -5.6372081884144265 1102 52-53 -1.9790204234015576 1102 54-55 0.6708283632917968 1102 56-57 -1.3371885307873868 1102 58-59 1.0383096264119658 1102 60-61 0.18328038110117717 1102 62-63 -4.495988089336457 1102 64-65 -0.6138644955354877 1102 66-67 -0.018320602293250943 1102 68-69 -8.13386298770758 1102 70-71 -0.3297007892793147 1102 72-73 0.41740029587919736 1102 74-75 -6.49857428128108 1102 76 -2.052134293332557 1103 1 -0.06181991333344783 1103 2 -0.12587044402354053 1103 3 -0.34407809596283556 1103 4 0.05153403020844394 1103 5 0.15900721393299477 1103 6 -0.8242274566405214 1103 7 0.6218232844781681 1103 8 1.2881453779638221 1103 9 0.022683170774996597 1103 10-11 0.5276586347250003 1103 12-13 -1.13685759612018 1103 14-15 -1.0105562748556096 1103 16-17 -0.7763905391666697 1103 18-19 -0.8202972554722194 1103 20-21 -0.8143276007584319 1103 22-23 -0.2760288772883115 1103 24-25 0.2823946594926312 1103 26-27 -1.1770897350895524 1103 28-29 0.5648959265058551 1103 30-31 0.23930651029878192 1103 32-33 -5.321389725885247 1103 34-35 -1.3433708120603782 1103 36-37 -0.21052914938410083 1103 38-39 0.2104392909436541 1103 40-41 0.5057538771103225 1103 42-43 0.412231823381795 1103 44-45 -5.439903309564087 1103 46-47 -0.35594942760629067 1103 48-49 -0.27022739134329754 1103 50-51 0.047315621109390804 1103 52-53 -0.3365884506124459 1103 54-55 0.2494848258768272 1103 56-57 0.9544781358792775 1103 58-59 0.7168810549833964 1103 60-61 0.8792837824617266 1103 62-63 0.15792347528939388 1103 64-65 0.5345198582060107 1103 66-67 -0.1628784254225053 1103 68-69 -2.8570772734218686 1103 70-71 0.011285605278509081 1103 72-73 0.5321962142465466 1103 74-75 -1.8548667982878797 1103 76 -0.5912499395910586 1104 1 -2.625148634652483 1104 2 0.1479102546966189 1104 3 -0.043691839765443774 1104 4 0.14936638348873998 1104 5 -0.25342216459807787 1104 6 -0.6265161687355274 1104 7 0.3795772335154126 1104 8 1.1041836576824906 1104 9 -1.068807662846588 1104 10-11 -1.018648871328267 1104 12-13 -1.3977390673527381 1104 14-15 -0.39940673029202145 1104 16-17 -0.6824351604049994 1104 18-19 0.21358663706784142 1104 20-21 0.2214731602239297 1104 22-23 -0.24112169407604256 1104 24-25 0.019495432005022906 1104 26-27 0.126265505311693 1104 28-29 -0.9728361154634726 1104 30-31 -1.1178795844781106 1104 32-33 -1.0947236357203707 1104 34-35 -0.6230894791882449 1104 36-37 -0.39845431945212795 1104 38-39 -0.1823025515560559 1104 40-41 0.5287851235071841 1104 42-43 0.5335422119440025 1104 44-45 -3.3045839275740043 1104 46-47 -0.30314186390283737 1104 48-49 0.45896089414038954 1104 50-51 0.4818394306331939 1104 52-53 -0.6823598100036605 1104 54-55 -1.204827407491095 1104 56-57 -0.20098998357189402 1104 58-59 0.5926881574854121 1104 60-61 -0.145462556751923 1104 62-63 0.44882627708807377 1104 64-65 -0.18161066179860086 1104 66-67 -0.03133513013829514 1104 68-69 -4.22592311684398 1104 70-71 -0.8599671331049805 1104 72-73 -0.19730914722007498 1104 74-75 -2.248069842217312 1104 76 -0.8907137929290059 1105 1 -1.1190108525363556 1105 2 -0.051663798014352835 1105 3 0.38941373008695734 1105 4 0.4077152461503175 1105 5 0.33178208304294543 1105 6 0.4595902589273777 1105 7 1.4227635558746883 1105 8 0.5656857134697546 1105 9 0.5721542681144527 1105 10-11 0.9651118882777254 1105 12-13 -1.4550940530065972 1105 14-15 -0.110620384269005 1105 16-17 0.7532734206580969 1105 18-19 0.3137581991703655 1105 20-21 -0.789752325622203 1105 22-23 0.5626023867356267 1105 24-25 0.5041731614693319 1105 26-27 0.9017114188798914 1105 28-29 -0.4412746045337812 1105 30-31 0.05658132598421872 1105 32-33 0.5900457907297749 1105 34-35 0.44415990703354424 1105 36-37 0.37728739084455043 1105 38-39 0.5409500292703981 1105 40-41 0.5966556828587954 1105 42-43 0.651239730209447 1105 44-45 1.4527000668650203 1105 46-47 0.4622469608967563 1105 48-49 0.07417371988653088 1105 50-51 0.17642930846914595 1105 52-53 -1.1545844971986199 1105 54-55 -0.7560446110288268 1105 56-57 0.2593584649742624 1105 58-59 0.2968174798151111 1105 60-61 0.04988160274167086 1105 62-63 0.5344594299854037 1105 64-65 0.20184542717705511 1105 66-67 0.027646737522253773 1105 68-69 1.5655106098990004 1105 70-71 1.5567365081527456 1105 72-73 0.44853342976102795 1105 74-75 -0.6874610925500875 1105 76 -1.3912018575310086 1106 1 -0.6326815913379846 1106 2 0.1599035146827461 1106 3 -0.5576178978485018 1106 4 -0.18733695678284334 1106 5 0.40462124902071395 1106 6 0.0707779139287581 1106 7 0.7381856188856162 1106 8 1.153940818947234 1106 9 1.6937059658579372 1106 10-11 0.2514472729873276 1106 12-13 0.571237045245141 1106 14-15 0.15867633046722318 1106 16-17 0.5822590610242813 1106 18-19 -0.07162497954430336 1106 20-21 -0.22386334488064108 1106 22-23 0.04205800658804293 1106 24-25 0.5823857085438249 1106 26-27 0.12557168035382205 1106 28-29 -1.180091542165826 1106 30-31 -0.398135308534016 1106 32-33 0.6459840657366698 1106 34-35 0.35014870100800266 1106 36-37 0.6921889554016687 1106 38-39 0.4422390283824882 1106 40-41 0.07482536535474082 1106 42-43 0.41955668315026884 1106 44-45 1.220542447686185 1106 46-47 0.6667263093551554 1106 48-49 0.2584454813145385 1106 50-51 0.3970441089957717 1106 52-53 -0.46340847686855824 1106 54-55 0.23029995894759736 1106 56-57 0.7682834825793634 1106 58-59 -0.3688541162780936 1106 60-61 0.4139090360719422 1106 62-63 0.021033631632633387 1106 64-65 0.6327416030443018 1106 66-67 0.7286509933625567 1106 68-69 1.2018199696859 1106 70-71 -0.1793248494296904 1106 72-73 0.16437189153501208 1106 74-75 -1.4608758685826686 1106 76 -0.924314744460375 1107 1 -0.04980363141084254 1107 2 0.09845207186805993 1107 3 0.3217800505744677 1107 4 -0.0715283094815291 1107 5 0.24643890792206236 1107 6 0.1590724206874583 1107 7 0.8040469936339676 1107 8 0.5385635780307325 1107 9 -0.7922493596142104 1107 10-11 -0.3530010061805413 1107 12-13 -0.06547084469829656 1107 14-15 -0.3908395357009269 1107 16-17 0.5374407310463312 1107 18-19 0.8735384755414941 1107 20-21 0.40809907482983476 1107 22-23 -0.997535512729371 1107 24-25 -0.7250632417006386 1107 26-27 0.31270618327779687 1107 28-29 -0.9567668369601918 1107 30-31 0.12214079155226187 1107 32-33 1.1530303700219662 1107 34-35 0.5057426037977635 1107 36-37 0.07968775705059983 1107 38-39 0.9362952070805974 1107 40-41 1.0376764822179396 1107 42-43 0.7903265038769476 1107 44-45 0.048986508658281025 1107 46-47 0.4026097337631711 1107 48-49 0.3266556241579792 1107 50-51 1.112986971616806 1107 52-53 0.8780736437334937 1107 54-55 0.4404907364377735 1107 56-57 0.9096889087130009 1107 58-59 0.892817823133278 1107 60-61 -0.21460213256002447 1107 62-63 -0.17602321814207755 1107 64-65 0.579295145370061 1107 66-67 1.2803718254975323 1107 68-69 1.3142732339942143 1107 70-71 0.23353106083778385 1107 72-73 0.5466938165661688 1107 74-75 0.09012595291095948 1107 76 -0.5751631770171741 1108 1 -0.8002898775903375 1108 2 0.3287012151485982 1108 3 -0.264030822816288 1108 4 -0.37605734532482415 1108 5 -0.21387414199920585 1108 6 0.47517874651870784 1108 7 1.5942664990521394 1108 8 0.8442966520327744 1108 9 -0.0688076628465879 1108 10-11 0.3372833320615598 1108 12-13 0.32542477445517193 1108 14-15 0.6712147386345393 1108 16-17 -0.8208532395010408 1108 18-19 0.13166573311303864 1108 20-21 0.6028290924273136 1108 22-23 -0.12530248503649233 1108 24-25 -0.8110130425712541 1108 26-27 -0.7494407093775735 1108 28-29 0.40287009922578676 1108 30-31 0.3708209804936402 1108 32-33 0.6199656298163561 1108 34-35 -0.06659230404699912 1108 36-37 0.25408805342922847 1108 38-39 0.778149425845065 1108 40-41 0.1728529201173501 1108 42-43 0.6465365622264869 1108 44-45 -0.2170133061050734 1108 46-47 0.5104174581310588 1108 48-49 0.04370665685225106 1108 50-51 0.37166993910777535 1108 52-53 0.24419386231272 1108 54-55 0.3092968862942129 1108 56-57 0.5052247056936423 1108 58-59 -0.9694587351699582 1108 60-61 0.8432380082198208 1108 62-63 -0.02010027658423752 1108 64-65 0.18844583537653392 1108 66-67 0.019512327488833137 1108 68-69 0.9294441147944354 1108 70-71 1.659806878194452 1108 72-73 0.6670976324409494 1108 74-75 1.5570149035453937 1108 76 0.7759528737376655 1109 1 -0.8401318161292473 1109 2 -1.1871539169502867 1109 3 0.22266131656517985 1109 4 -0.06094680027403854 1109 5 0.11854478618732855 1109 6 0.4553230919025921 1109 7 -1.0238060655277295 1109 8 0.6514224807124407 1109 9 0.622482628130193 1109 10-11 0.7383316322477143 1109 12-13 0.06254962341503045 1109 14-15 -0.02972579237359696 1109 16-17 -1.1534844403109403 1109 18-19 0.25011385694962485 1109 20-21 0.5705963110028023 1109 22-23 0.5639793803499984 1109 24-25 0.48386628860669845 1109 26-27 0.3589489578442695 1109 28-29 0.1613038538386391 1109 30-31 0.495131094467709 1109 32-33 0.6785365452012257 1109 34-35 0.03942970699117865 1109 36-37 0.18343392717599727 1109 38-39 0.5606516302146218 1109 40-41 -0.19039452444683036 1109 42-43 0.2416673548198034 1109 44-45 -0.01827714464725716 1109 46-47 -0.024346266110477188 1109 48-49 -0.0575637131552611 1109 50-51 1.0203750761052532 1109 52-53 0.8761784480545387 1109 54-55 0.16475555844731815 1109 56-57 0.6959108361270054 1109 58-59 0.09972581138305969 1109 60-61 0.9341233483458851 1109 62-63 -0.29164594785558506 1109 64-65 0.09008196744276376 1109 66-67 0.012817575521218316 1109 68-69 0.5954413062643411 1109 70-71 0.2976044680837475 1109 72-73 -0.11408195019841827 1109 74-75 0.8467670344359632 1109 76 -0.3926242465392704 1110 1 0.061031858954414986 1110 2 0.06048831000438071 1110 3 -0.4713605879069789 1110 4 0.06120105491306305 1110 5 0.36295458235404965 1110 6 0.40630422971823066 1110 7 0.4640628118680752 1110 8 1.8374203511109783 1110 9 -0.710533800223935 1110 10-11 -1.093581966778757 1110 12-13 -0.2723302062168429 1110 14-15 -0.4604757163164024 1110 16-17 0.23320935342194815 1110 18-19 0.03838964033872827 1110 20-21 -0.660997847804623 1110 22-23 -0.5452957945815413 1110 24-25 -0.4033599054912642 1110 26-27 -1.266241185143258 1110 28-29 -0.1066266298851204 1110 30-31 -0.2753282909901529 1110 32-33 0.5670366973156149 1110 34-35 0.4696662448676534 1110 36-37 -0.5322262492766896 1110 38-39 -0.03217617629587011 1110 40-41 -0.060774049849939615 1110 42-43 0.19002451940757226 1110 44-45 0.8587003424230204 1110 46-47 0.13565905789317867 1110 48-49 0.4795712123086844 1110 50-51 0.27898855344021456 1110 52-53 -0.09571841838903339 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0.929131887444413 2116 38-39 0.5987221903102906 2116 40-41 1.4264083457943428 2116 42-43 1.0235090929102952 2116 44-45 2.200578745326837 2116 46-47 0.7222818649107197 2116 48-49 1.294339814849522 2116 50-51 1.3439083961504394 2116 52-53 1.5545386898989335 2116 54-55 1.0262265570520555 2116 56-57 0.7091989897380628 2116 58-59 0.3587119252625399 2116 60-61 -0.7800816878643317 2116 62-63 0.6466637990050828 2116 64-65 1.373717606270752 2116 66-67 0.7889454075589626 2116 68-69 1.0632839745255893 2116 70-71 -0.9479273902645389 2116 72-73 1.0062755011337217 2116 74-75 -0.016431424138225736 2116 76 0.5094420613472508 2117 1 0.649064440408047 2117 2 0.6513404403301948 2117 3 1.0674865298875034 2117 4 0.18588777170504045 2117 5 -0.6028975479717644 2117 6 0.20298342771322098 2117 7 -1.0431427019163877 2117 8 -1.6710382955055607 2117 9 0.43764915716955244 2117 10-11 0.3074205394869054 2117 12-13 0.8316798188458066 2117 14-15 -0.2452501524066335 2117 16-17 0.6904461955272083 2117 18-19 0.4135462819427431 2117 20-21 0.49689688903748674 2117 22-23 1.093018741759309 2117 24-25 1.2211701696967054 2117 26-27 1.7115157070873224 2117 28-29 0.7910864026963296 2117 30-31 0.577741884448443 2117 32-33 1.134392587039926 2117 34-35 0.7408128614090046 2117 36-37 -0.09488289088070445 2117 38-39 0.8754052773382099 2117 40-41 -0.5274093881958066 2117 42-43 -0.1285845031488151 2117 44-45 1.2709810441774039 2117 46-47 0.6329961506250044 2117 48-49 -0.15373079270383982 2117 50-51 0.6068394306331939 2117 52-53 1.2680033861222526 2117 54-55 -0.20119544623201335 2117 56-57 -0.20028376888262045 2117 58-59 0.12164295974529438 2117 60-61 1.0195899049107098 2117 62-63 0.4504404820921195 2117 64-65 0.3355402663692715 2117 66-67 0.11263177865912866 2117 68-69 0.5060179646733687 2117 70-71 0.9738706392921088 2117 72-73 0.9947812482601606 2117 74-75 0.19785429014749667 2117 76 0.20977046857220927 2118 1 0.37741941876302576 2118 2 0.8732019121916679 2118 3 0.7222484346494085 2118 4 0.9856713214885886 2118 5 0.12004617497195369 2118 6 1.2949747697045595 2118 7 1.202528293754611 2118 8 -1.846362970830242 2118 9 -0.3177620982417011 2118 10-11 -0.0259127938464232 2118 12-13 1.2223724595384553 2118 14-15 -0.6997956069520868 2118 16-17 0.4307059357869534 2118 18-19 0.46657658497304766 2118 20-21 -1.8245316823910827 2118 22-23 0.5129321616727296 2118 24-25 0.8883779619045029 2118 26-27 0.5789399495115646 2118 28-29 0.33437644598637206 2118 30-31 0.39213582384238066 2118 32-33 0.7604748381221782 2118 34-35 1.4404881860843304 2118 36-37 0.6946192736214627 2118 38-39 0.14596804790097906 2118 40-41 -0.6978639336503463 2118 42-43 -0.08150658107089015 2118 44-45 1.328881477077843 2118 46-47 0.2525848952137508 2118 48-49 0.06975405578100435 2118 50-51 -0.061450612656841486 2118 52-53 -0.7969316788128182 2118 54-55 0.3009690559324838 2118 56-57 0.4496080060091501 2118 58-59 0.7801927432950819 2118 60-61 -1.3895010041802038 2118 62-63 0.5521720838237201 2118 64-65 0.22839740922641738 2118 66-67 -1.2136669226395753 2118 68-69 1.3701954538508616 2118 70-71 -0.6067570663355895 2118 72-73 -0.8082490547701511 2118 74-75 1.0398456321388352 2118 76 0.8266535854553254 2119 1 0.6371596785032878 2119 2 0.21086424985400498 2119 3 -0.21425950185852827 2119 4 0.2771576129748823 2119 5 0.46059451552029884 2119 6 0.8577453324751261 2119 7 -1.1899680987417867 2119 8 1.8765807521134832 2119 9 0.44558566510605857 2119 10-11 0.7518649839313483 2119 12-13 0.748346485512478 2119 14-15 0.17538476822829097 2119 16-17 0.19044619552720832 2119 18-19 1.0444986628951227 2119 20-21 0.6437222858628786 2119 22-23 1.5374631862037518 2119 24-25 0.8025193760459146 2119 26-27 0.6063569769285877 2119 28-29 1.164102275712203 2119 30-31 0.8654402971468542 2119 32-33 1.00145607910342 2119 34-35 1.0602573058534475 2119 36-37 0.31583139483358025 2119 38-39 0.5023894043223365 2119 40-41 -0.05717129295770462 2119 42-43 -0.20199720156151812 2119 44-45 0.7293143775107396 2119 46-47 0.4683136109424595 2119 48-49 -0.05650857048161839 2119 50-51 0.8469187957125612 2119 52-53 -0.3510442329253678 2119 54-55 -0.6932589382955072 2119 56-57 0.4445575009586449 2119 58-59 1.1315635946659341 2119 60-61 1.1941930795138802 2119 62-63 1.0456785773302073 2119 64-65 1.2522069330359429 2119 66-67 0.9479492389765838 2119 68-69 -0.03168044802504255 2119 70-71 1.209981750403223 2119 72-73 -0.8068060533271435 2119 74-75 1.166108258401458 2119 76 2.6621514209531583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 4.0 13 26.0 14 52.0 15 108.0 16 117.0 17 131.0 18 151.0 19 166.0 20 189.0 21 179.0 22 174.0 23 198.0 24 252.0 25 381.0 26 417.0 27 531.0 28 707.0 29 873.0 30 1116.0 31 1468.0 32 1969.0 33 2677.0 34 4001.0 35 6620.0 36 12424.0 37 16991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.43599185967632 22.651419711212327 10.018412636883419 27.894175792227927 2 19.993066656395047 19.37291758950754 34.60123644627622 26.032779307821198 3 18.00358222752923 23.184330643452807 29.66315505652601 29.148932072491956 4 13.310093792731545 16.99824740481097 38.3991679987674 31.292490803690082 5 12.645648363923504 36.83146197253625 36.267164840244206 14.255724823296035 6 28.89663540242282 38.0370933882865 17.185062496388884 15.881208712901799 7 28.164782466344395 30.82449011035572 21.86121757217418 19.149509851125707 8 24.80211081794195 34.056198601775705 19.184176569150473 21.957514011131867 9 23.910405793193767 14.949059183791382 21.431735454422896 39.70879956859195 10-11 24.785740423319147 24.986999980740713 28.029004487414056 22.198255108526084 12-13 23.98936887313907 23.650405408008012 26.637520944475472 25.72270477437744 14-15 21.28247597403848 25.011074090480136 24.93211101053483 28.77433892494656 16-17 22.455366600543112 28.060782312270092 26.470928105078674 23.012922982108122 18-19 21.689809910829496 27.501300001925927 27.132484640717987 23.676405446526587 20-21 21.354698303256743 27.500337037536355 27.252855189415094 23.892109469791805 22-23 21.986402942819176 27.123817961211792 27.91344876066483 22.9763303353042 24-25 21.939217687729908 27.61107794233769 27.52055928971747 22.929145080214933 26-27 20.772104847562737 28.196560291200427 27.842189395836144 23.18914546540069 28-29 21.690772875219075 27.394410954682897 28.507597789033763 22.40721838106427 30-31 21.54729118117212 27.64381873158331 27.717966989580727 23.090923097663847 32-33 20.867438322130845 27.918263582612713 27.654411339868652 23.559886755387787 34-35 22.229069968992548 27.767078173449143 27.69774473739961 22.306107120158696 36-37 22.426477668855807 27.34048494886659 26.934113976465152 23.298923405812452 38-39 21.50684667680989 27.92981915528764 28.439227317373806 22.12410685052867 40-41 22.226181075823817 26.657743196656586 28.33330123452035 22.78277449299925 42-43 21.274772258921864 27.317373803516748 28.081004564451206 23.326849373110182 44-45 21.31040194133621 27.249966296246363 28.03670820253067 23.402923559886755 46-47 21.253587042351175 26.907150973557 28.260115940912506 23.57914604317932 48-49 20.633437975463668 27.271151512817056 28.98233923309516 23.113071278624115 50-51 20.65077133447605 27.15559578606783 28.640486874795368 23.553146004660746 52-53 20.581437898426515 26.587446796217474 28.882190936579168 23.94892436877684 54-55 19.901585039385246 27.50418889509466 28.77530188933613 23.818924176183963 56-57 19.839955318452322 26.973595516437804 29.015080022340772 24.1713691427691 58-59 20.43314138243168 26.791595246807777 28.58174604703118 24.19351732372937 60-61 20.184696569920842 26.65196541031913 28.938042871174623 24.225295148585406 62-63 19.54817710841053 27.06218824027887 29.515821504920748 23.873813146389846 64-65 20.100918668027656 27.395373919072476 28.010708164012094 24.492999248887777 66-67 19.901585039385246 26.94952140669838 28.075226778113745 25.073666775802632 68-69 19.589584577162338 26.553743042582283 28.381449453999192 25.475222926256187 70-71 20.97047551181557 26.771372994626656 27.336633091308283 24.921518402249486 72-73 20.599734221828477 26.086705313637502 27.453151782447083 25.860408682086938 74-75 21.02632744641103 26.533520790401173 27.17966989580725 25.26048186738055 76 20.88862353870154 25.80744564066021 27.27307744159621 26.03085337904204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 1.5 16 3.0 17 3.0 18 3.5 19 7.0 20 10.5 21 11.0 22 18.0 23 35.0 24 49.5 25 54.0 26 85.0 27 167.0 28 257.5 29 297.0 30 345.5 31 456.0 32 622.5 33 727.0 34 885.5 35 1200.0 36 1428.5 37 1501.0 38 1767.5 39 2311.0 40 2846.5 41 3301.5 42 3498.0 43 3534.5 44 3784.5 45 3983.5 46 3969.0 47 3973.5 48 3824.5 49 3481.5 50 3292.0 51 3079.0 52 2580.5 53 2123.0 54 1951.0 55 1793.5 56 1413.0 57 1002.5 58 815.0 59 640.5 60 380.0 61 232.5 62 171.0 63 120.0 64 55.0 65 32.0 66 19.5 67 16.0 68 13.0 69 10.0 70 9.0 71 8.0 72 6.5 73 5.5 74 4.0 75 2.0 76 2.0 77 2.0 78 1.0 79 0.0 80 1.0 81 1.0 82 1.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6317046395624291 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.13993798509331 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75155691003049 43.46050883038345 2 11.80270306081887 13.724168480249602 3 4.7005432622234 8.1986788128575 4 2.633496753676958 6.124453517708915 5 1.7556645024513051 5.10371126475743 6 1.2620908970451836 4.402673189145466 7 0.718828673645157 2.9254858155345413 8 0.6161388631244203 2.8657820233807754 9 0.367695773154896 1.9240028503745934 >10 1.3912813038293361 11.270535215607728 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0019259287791537469 0.0 10 0.0 0.0 0.0 0.0019259287791537469 0.0 11 0.0 0.0 0.0 0.0019259287791537469 0.0 12 0.0 0.0 0.0 0.0019259287791537469 0.0 13 0.0 0.0 0.0 0.0019259287791537469 0.0 14 0.0 0.0 0.0 0.0038518575583074938 0.0 15 0.0 0.0 0.0 0.00577778633746124 0.0 16 0.0 0.0 0.0 0.00577778633746124 0.0 17 0.0 0.0 0.0 0.009629643895768734 0.0 18 0.0 0.0 0.0 0.009629643895768734 0.0 19 0.0 0.0 0.0 0.01155557267492248 0.0 20 0.0 0.0 0.0 0.013481501454076228 0.0 21 0.0 0.0 0.0 0.015407430233229975 0.0 22 0.0 0.0 0.0 0.017333359012383722 0.0 23 0.0 0.0 0.0 0.017333359012383722 0.0 24 0.0 0.0 0.0 0.017333359012383722 0.0 25 0.0 0.0 0.0 0.019259287791537467 0.0 26 0.0 0.0 0.0 0.02311114534984496 0.0 27 0.0 0.0 0.0 0.03081486046645995 0.0 28 0.0 0.0 0.0 0.038518575583074935 0.0 29 0.0 0.0 0.0 0.04237043314138243 0.0 30 0.0 0.0 0.0 0.06355564971207364 0.0 31 0.0 0.0 0.0 0.07511122238699613 0.0 32 0.0 0.0 0.0 0.08474086628276487 0.0 33 0.0 0.0 0.0 0.10207422529514859 0.0 34 0.0 0.0 0.0 0.13866687209906978 0.0 35 0.0 0.0 0.0 0.19644473547368219 0.0 36 0.0 0.0 0.0 0.26963002908152456 0.0 37 0.0 0.0 0.0 0.4275561889721318 0.0 38 0.0 0.0 0.0 0.6721491439246576 0.0 39 0.0 0.0 0.0 0.9398532442270285 0.0 40 0.0 0.0 0.0 1.2807426381372418 0.0 41 0.0 0.0 0.0 1.6177801744891473 0.0 42 0.0 0.0 0.0 2.0954105117192765 0.0 43 0.0 0.0 0.0 2.6288927835448646 0.0 44 0.0 0.0 0.0 3.1315601949039924 0.0 45 0.0 0.0 0.0 3.6380794638214278 0.0 46 0.0 0.0 0.0 4.148450590297171 0.0 47 0.0 0.0 0.0 4.708895865030911 0.0 48 0.0 0.0 0.0 5.1749706295861175 0.0 49 0.0 0.0 0.0 5.6949713999576295 0.0 50 0.0 0.0 0.0 6.2284536717832175 0.0 51 0.0 0.0 0.0 6.802380447971034 0.0 52 0.0 0.0 0.0 7.5303815264911504 0.0 53 0.0 0.0 0.0 8.237197388440576 0.0 54 0.0 0.0 0.0 8.92860582015677 0.0 55 0.0 0.0 0.0 9.598829035302275 0.0 56 0.0 0.0 0.0 10.22860774608555 0.0 57 0.0 0.0 0.0 10.781349305702674 0.0 58 0.0 0.0 0.0 11.384165013577798 0.0 59 0.0 0.0 0.0 11.950388074649 0.0 60 0.0 0.0 0.0 12.530092637174278 0.0 61 0.0 0.0 0.0 13.18490842208655 0.0 62 0.0 0.0 0.0 13.770390770949291 0.0 63 0.0 0.0 0.0 14.40209541051172 0.0 64 0.0 0.0 0.0 15.060763052982301 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGCCG 15 0.0022138876 69.992294 64 TAGAGCT 15 0.0022138876 69.992294 4 TTCTATC 25 2.3710438E-4 55.993835 30 ATCGCCT 25 2.3710438E-4 55.993835 34 TCTATCG 25 2.3710438E-4 55.993835 31 CGGGTGA 20 0.0069178934 52.49422 55 GACCCCG 20 0.0069178934 52.49422 7 GCGAGGA 20 0.0069178934 52.49422 23 CCGTGGG 30 5.830545E-4 46.66153 9 TCTAGAA 30 5.830545E-4 46.66153 2 TAAATGG 30 5.830545E-4 46.66153 55 TATCGCC 30 5.830545E-4 46.66153 33 AATTTCT 35 0.0012453987 39.995594 27 TACTTTA 35 0.0012453987 39.995594 42 CTATCGC 35 0.0012453987 39.995594 32 GGGTAAA 35 0.0012453987 39.995594 52 CCTACTA 35 0.0012453987 39.995594 4 CTAAGGT 35 0.0012453987 39.995594 67 TAGCCCC 35 0.0012453987 39.995594 53 TATTGCG 35 0.0012453987 39.995594 11 >>END_MODULE