FastQCFastQC Report
Wed 25 May 2016
SRR1780538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51923
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT1050.20222252181114342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG150.002212921670.048
CAAACGA150.002212921670.049
GCGCCTT150.002212921670.011
TAGGCCA150.002212921670.05
AAGATAG207.8576166E-570.05
GCTGTCG150.002212921670.054
AAAGCGC150.002212921670.08
GACTATC150.002212921670.038
AAGCTAC207.8576166E-570.06
CTCATAG252.3697535E-456.0000041
CCATTTA252.3697535E-456.00000413
CTGTCGC200.006914880652.555
GCCTTCC200.006914880652.513
GCCTTAA200.006914880652.560
GGTACCG200.006914880652.558
AAATCGC200.006914880652.549
CTCCGGT200.006914880652.57
ACTGACT200.006914880652.535
AACCCGT200.006914880652.510
CGTTGAA200.006914880652.542
Produced by FastQC (version 0.11.5)