##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51923 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.302486374053885 34.0 34.0 34.0 27.0 34.0 2 33.08961346609402 34.0 34.0 34.0 32.0 34.0 3 33.30637675018778 34.0 34.0 34.0 33.0 34.0 4 33.26545076363076 34.0 34.0 34.0 33.0 34.0 5 33.292837470870325 34.0 34.0 34.0 33.0 34.0 6 36.912524314850835 38.0 37.0 38.0 35.0 38.0 7 36.57523640775764 38.0 37.0 38.0 34.0 38.0 8 36.46480365156096 38.0 37.0 38.0 34.0 38.0 9 36.35261830017526 38.0 37.0 38.0 34.0 38.0 10-11 35.968886620572775 38.0 36.5 38.0 32.5 38.0 12-13 35.88722724033665 38.0 36.0 38.0 31.5 38.0 14-15 36.173641738728506 38.0 37.0 38.0 33.0 38.0 16-17 36.34014791133024 38.0 37.0 38.0 34.0 38.0 18-19 36.31508194826955 38.0 37.0 38.0 34.0 38.0 20-21 36.02630818712324 38.0 37.0 38.0 33.0 38.0 22-23 36.02052077114189 38.0 36.5 38.0 32.5 38.0 24-25 35.24943666583209 37.5 35.5 38.0 29.0 38.0 26-27 35.554677118040175 37.5 36.0 38.0 31.0 38.0 28-29 35.34393236138128 38.0 36.0 38.0 29.0 38.0 30-31 34.95121622402404 37.0 35.5 38.0 27.0 38.0 32-33 35.53567783063383 38.0 36.0 38.0 31.0 38.0 34-35 35.64189280280415 38.0 36.5 38.0 32.0 38.0 36-37 35.83243456656973 38.0 37.0 38.0 32.0 38.0 38-39 35.56704158080234 38.0 36.5 38.0 30.0 38.0 40-41 34.85629682414344 37.0 34.5 38.0 27.0 38.0 42-43 35.27534040791171 37.0 35.5 38.0 29.0 38.0 44-45 35.49934518421509 38.0 36.0 38.0 31.0 38.0 46-47 35.81314638984651 38.0 36.5 38.0 32.0 38.0 48-49 35.53189338058279 38.0 36.0 38.0 31.0 38.0 50-51 35.81234712940315 38.0 36.0 38.0 32.0 38.0 52-53 35.69146620957957 38.0 36.0 38.0 31.0 38.0 54-55 35.85855016081506 38.0 36.5 38.0 32.0 38.0 56-57 35.95224659592088 38.0 37.0 38.0 33.0 38.0 58-59 36.07250158889124 38.0 37.0 38.0 34.0 38.0 60-61 35.595814956762894 38.0 36.5 38.0 30.0 38.0 62-63 35.90330874564259 38.0 36.5 38.0 32.5 38.0 64-65 35.962964389576875 38.0 37.0 38.0 33.0 38.0 66-67 35.30504785933016 38.0 36.0 38.0 29.0 38.0 68-69 34.77030410415423 37.0 34.5 38.0 27.0 38.0 70-71 34.83008493345916 37.0 35.0 38.0 27.0 38.0 72-73 34.542774878185 37.0 34.5 38.0 26.0 38.0 74-75 35.42346359031643 37.5 36.0 38.0 31.0 38.0 76 34.85393756138898 37.0 35.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16930321835058493 1101 2 0.30386941389382116 1101 3 0.3497830754272968 1101 4 0.4962122928528814 1101 5 0.4371345822887136 1101 6 -0.6801604593805877 1101 7 0.6436945377261267 1101 8 0.6821407929629473 1101 9 0.4198576386941113 1101 10-11 0.2521264476133993 1101 12-13 0.6940716941072367 1101 14-15 0.8574179346422994 1101 16-17 -0.4758408196286936 1101 18-19 -0.534427349069567 1101 20-21 0.09160358391078915 1101 22-23 1.1411943708733716 1101 24-25 -1.5802097782135434 1101 26-27 -0.30927030482203577 1101 28-29 0.7626814128316539 1101 30-31 1.0534078088891476 1101 32-33 0.04761273673817357 1101 34-35 0.22874388362882314 1101 36-37 -0.12233254869954635 1101 38-39 0.49186745297289747 1101 40-41 1.545243244218689 1101 42-43 0.7163076678233651 1101 44-45 0.5501071618442452 1101 46-47 0.712157618201374 1101 48-49 -1.2097074868617526 1101 50-51 -0.963381213615591 1101 52-53 -1.967746656243122 1101 54-55 -1.5175526354564752 1101 56-57 -1.367516777392673 1101 58-59 -1.149057207509351 1101 60-61 -2.3370260795958018 1101 62-63 -1.1514830639140996 1101 64-65 -1.8265629632374285 1101 66-67 -1.4244409262385247 1101 68-69 -1.017738697213808 1101 70-71 -1.2746283611371751 1101 72-73 -1.1905072079811987 1101 74-75 -0.654972063967115 1101 76 -0.9869869016975272 1102 1 -0.7881143640669954 1102 2 0.44397930400371166 1102 3 0.5723105479547712 1102 4 0.7723111939517793 1102 5 0.030083300237436106 1102 6 0.31983954061941233 1102 7 0.5580718271034115 1102 8 0.6638257746479255 1102 9 1.050810019646491 1102 10-11 0.8814762644632239 1102 12-13 1.433540558576098 1102 14-15 0.6424454071697738 1102 16-17 0.7645437957559196 1102 18-19 0.1416257644835497 1102 20-21 1.0048361846433878 1102 22-23 1.00863942581843 1102 24-25 -4.852645675649438 1102 26-27 -0.56247543302716 1102 28-29 0.4911612663115079 1102 30-31 2.304552497533841 1102 32-33 1.3182171323425749 1102 34-35 1.6525076198925603 1102 36-37 -2.313266614633605 1102 38-39 0.33138210498754006 1102 40-41 1.5353989218743678 1102 42-43 1.3461153601310585 1102 44-45 1.390079689316778 1102 46-47 1.8101429661867172 1102 48-49 -2.9828301974844607 1102 50-51 -0.26008451031889024 1102 52-53 0.44937788588141814 1102 54-55 1.031668976265145 1102 56-57 1.2865583141824146 1102 58-59 0.9003933419412036 1102 60-61 -4.942337434907159 1102 62-63 -1.7174171298481653 1102 64-65 0.7560835569090898 1102 66-67 1.79511035581276 1102 68-69 -0.11251891699402705 1102 70-71 -0.7730257970346059 1102 72-73 -5.474847867321856 1102 74-75 -1.6110160200110712 1102 76 0.48462481991419537 1103 1 0.8725999216472857 1103 2 0.7330949502622204 1103 3 0.4260520445534084 1103 4 0.7799642551762673 1103 5 0.43909690567961235 1103 6 0.5307238943609107 1103 7 1.1728677454707608 1103 8 0.7148461828111934 1103 9 0.6375447135240435 1103 10-11 0.7943164005176442 1103 12-13 -0.346221346185807 1103 14-15 0.08844880853031611 1103 16-17 0.9567206665042178 1103 18-19 1.1114386896536175 1103 20-21 -0.005793067057290102 1103 22-23 0.9895067727572027 1103 24-25 -2.765060641635827 1103 26-27 -0.2716591064965428 1103 28-29 0.4758551438625318 1103 30-31 1.4852497764454 1103 32-33 1.840325975879992 1103 34-35 1.0202797287360994 1103 36-37 -0.4663278391234087 1103 38-39 0.4210929893412896 1103 40-41 0.5528308946634795 1103 42-43 0.3831051560494245 1103 44-45 1.77655928115351 1103 46-47 1.584802830132297 1103 48-49 -1.432234959389227 1103 50-51 0.6030107277763506 1103 52-53 0.4034595185344827 1103 54-55 0.5337948266052805 1103 56-57 0.7827317835701706 1103 58-59 0.28049538275752894 1103 60-61 -3.7161469587166778 1103 62-63 -0.5660565856304771 1103 64-65 0.9997060058886831 1103 66-67 0.7224062741801092 1103 68-69 0.6102701986522376 1103 70-71 0.9876034546660719 1103 72-73 -4.088793445553151 1103 74-75 -1.5633969723920274 1103 76 -2.8946268807660793 1104 1 0.1270389854083831 1104 2 0.4574982709125095 1104 3 0.5499134535237786 1104 4 0.13843306642958453 1104 5 -0.6697754568247163 1104 6 0.32831411689060275 1104 7 0.3969539901381225 1104 8 0.7844875986995845 1104 9 1.1698576386941113 1104 10-11 -0.16367103174339803 1104 12-13 0.5007318418690758 1104 14-15 0.9212488777105321 1104 16-17 0.33799012343954615 1104 18-19 0.7911919468887092 1104 20-21 -0.40986316967460823 1104 22-23 0.14065112880471276 1104 24-25 0.42701534129971463 1104 26-27 1.1048974483287708 1104 28-29 1.6280660282162671 1104 30-31 0.9712696081068799 1104 32-33 1.016865235651693 1104 34-35 0.17344184103864535 1104 36-37 1.592302392630316 1104 38-39 1.5042533721384785 1104 40-41 1.951914256821901 1104 42-43 0.8690673375321936 1104 44-45 0.6451745238607671 1104 46-47 0.9716643544030177 1104 48-49 -0.039781367783085386 1104 50-51 0.5628573783009614 1104 52-53 -0.3095002416407766 1104 54-55 0.2767759173466615 1104 56-57 0.06213337471510272 1104 58-59 -0.35717324401521466 1104 60-61 0.5198296353107636 1104 62-63 0.2255812559468282 1104 64-65 0.752804702994645 1104 66-67 -0.11908455944147534 1104 68-69 1.7697450055241362 1104 70-71 2.141169118219622 1104 72-73 2.0066896629444884 1104 74-75 0.3325876926604394 1104 76 -0.2163437030882278 1105 1 1.2261900487976263 1105 2 0.46610595571151947 1105 3 0.43724447964262936 1105 4 -0.05463345825464927 1105 5 -0.691339038331499 1105 6 0.33118334515693704 1105 7 1.0409626274025925 1105 8 -0.2610059381418992 1105 9 0.9988279702822425 1105 10-11 0.9807813006138062 1105 12-13 -0.24852127139148905 1105 14-15 0.3207519392066729 1105 16-17 0.17466595980479127 1105 18-19 0.9571487014070144 1105 20-21 0.5404725596122191 1105 22-23 0.4352942500517898 1105 24-25 1.028680676881109 1105 26-27 0.520639750218912 1105 28-29 1.3130573022127834 1105 30-31 1.7651140781870467 1105 32-33 1.213910226334093 1105 34-35 -0.4744713130418674 1105 36-37 0.35315073764761706 1105 38-39 -0.09458710468585707 1105 40-41 0.6175012654295884 1105 42-43 0.7829609098692814 1105 44-45 1.6323665255172202 1105 46-47 1.2813783189665742 1105 48-49 0.0701958558688176 1105 50-51 1.1548057913515208 1105 52-53 0.366543181318967 1105 54-55 0.9222043789078711 1105 56-57 0.9863058833671587 1105 58-59 0.7808159298245201 1105 60-61 1.4698104085234078 1105 62-63 0.04956741265993969 1105 64-65 0.9906104828627207 1105 66-67 0.6841810364410321 1105 68-69 1.4699106516920821 1105 70-71 2.1293520389165224 1105 72-73 1.819997557759173 1105 74-75 0.42267258632151083 1105 76 1.4022264155262576 1106 1 -1.445069251285041 1106 2 0.29464764151416034 1106 3 0.2604893282388119 1106 4 0.3352811187638096 1106 5 0.12584353415556393 1106 6 0.24089217219836456 1106 7 -1.1251988495883154 1106 8 0.47543813889186737 1106 9 -0.2278031800193432 1106 10-11 1.0052753454991432 1106 12-13 1.5152030481333298 1106 14-15 -0.20190505231226297 1106 16-17 0.34056718756878723 1106 18-19 0.3754916792705174 1106 20-21 0.4543453742841521 1106 22-23 0.8300157833789967 1106 24-25 -0.1596632195090848 1106 26-27 1.372466087440678 1106 28-29 0.7684169054092536 1106 30-31 1.3113925142422787 1106 32-33 0.7658988366032276 1106 34-35 1.397547302432237 1106 36-37 1.6775437445977985 1106 38-39 0.6551603004762683 1106 40-41 1.2093233161934975 1106 42-43 1.1776212080842683 1106 44-45 1.6233190376877005 1106 46-47 1.4340986888266514 1106 48-49 1.6427441550635749 1106 50-51 0.8951995331230478 1106 52-53 0.8931957639098229 1106 54-55 0.19713472396772858 1106 56-57 0.7178970777580247 1106 58-59 1.337841128073201 1106 60-61 1.7463634840569213 1106 62-63 1.3983932293832524 1106 64-65 -0.27623682738497735 1106 66-67 0.7633121101987257 1106 68-69 2.1568002141672125 1106 70-71 0.7646204017957956 1106 72-73 0.9047469530624355 1106 74-75 0.36465231749937743 1106 76 0.4708403587613148 1107 1 1.1425062448322976 1107 2 -0.31929157811962483 1107 3 0.23565949409059783 1107 4 0.42990291916645873 1107 5 0.07858056799699398 1107 6 0.5411510160292536 1107 7 1.0160148403742966 1107 8 0.20988354201716675 1107 9 -0.33833908261736667 1107 10-11 0.8314665064616591 1107 12-13 0.5637122994035764 1107 14-15 0.39971511833292794 1107 16-17 1.138860735646638 1107 18-19 1.029359956521013 1107 20-21 1.173308081598897 1107 22-23 0.5429388013063274 1107 24-25 0.6118351986675066 1107 26-27 0.05432784566136206 1107 28-29 1.46139936154961 1107 30-31 1.1124662368000386 1107 32-33 1.702194493778947 1107 34-35 0.7700427486981809 1107 36-37 1.5645632058191623 1107 38-39 0.7430234008501486 1107 40-41 1.2960839335839651 1107 42-43 1.2446809339015559 1107 44-45 1.0863618907219248 1107 46-47 1.4739798124006143 1107 48-49 1.3579680070432545 1107 50-51 1.3288108839043673 1107 52-53 1.279783818746374 1107 54-55 1.1216865406679517 1107 56-57 1.381064559303418 1107 58-59 1.2146985722300414 1107 60-61 1.6770029241873061 1107 62-63 0.8345930184734627 1107 64-65 1.096881761436805 1107 66-67 0.6547415033537405 1107 68-69 1.5829338542784157 1107 70-71 0.91652338329326 1107 72-73 1.5554995175337254 1107 74-75 -0.017046464975948084 1107 76 1.6715881298283222 1108 1 -0.324938415721558 1108 2 0.00552086978256483 1108 3 -0.020708015402775004 1108 4 0.6243087726442766 1108 5 0.036439232440820035 1108 6 -0.47394577011505135 1108 7 0.29525907488388015 1108 8 -0.9443259606224501 1108 9 -0.6211028132832936 1108 10-11 0.3250295332283528 1108 12-13 1.4640086780272696 1108 14-15 0.47774605285177074 1108 16-17 0.21934605564293008 1108 18-19 0.7968416644028409 1108 20-21 1.047763948969461 1108 22-23 0.08697881242053285 1108 24-25 0.9637385051415208 1108 26-27 -0.6945375799198104 1108 28-29 -1.4708040282809023 1108 30-31 0.27635435386959273 1108 32-33 1.1778821848042327 1108 34-35 0.7949107675923202 1108 36-37 1.3832628446077138 1108 38-39 1.3884341630989212 1108 40-41 1.3276204715111746 1108 42-43 -0.14788181501018727 1108 44-45 0.7496942978720611 1108 46-47 1.0112123770018897 1108 48-49 1.1155858638553298 1108 50-51 0.9696370393179166 1108 52-53 0.2667709447999016 1108 54-55 -0.35599244423526244 1108 56-57 0.533884787144487 1108 58-59 0.5637307107870413 1108 60-61 1.0509030816384453 1108 62-63 1.1916829508620808 1108 64-65 1.0889628950850394 1108 66-67 1.519333519654566 1108 68-69 1.891213932077811 1108 70-71 -0.36165574053742944 1108 72-73 -0.10065496982387145 1108 74-75 -0.6787117423678097 1108 76 1.1621873703580974 1109 1 -1.014889756304818 1109 2 0.4696467964342119 1109 3 0.33029293165969875 1109 4 0.5089957521681328 1109 5 0.4055649958058325 1109 6 -0.1994282821166138 1109 7 -0.047316281897408885 1109 8 0.8293914228725328 1109 9 1.0214954036266732 1109 10-11 1.2308947890985067 1109 12-13 1.2135433111222085 1109 14-15 0.8583998525317327 1109 16-17 0.9687827167578504 1109 18-19 0.8978017898069695 1109 20-21 1.2829293583290493 1109 22-23 0.7413499955954777 1109 24-25 0.06835625113284038 1109 26-27 0.26194653229075726 1109 28-29 -0.7254985189898946 1109 30-31 0.9140866291055403 1109 32-33 0.9555347761631054 1109 34-35 0.36254409112845565 1109 36-37 1.3671627825587933 1109 38-39 1.5825003268427622 1109 40-41 0.87779845394153 1109 42-43 1.4375170942351048 1109 44-45 1.2309653210261047 1109 46-47 1.1328445901889168 1109 48-49 1.4494743717420775 1109 50-51 0.6643236922685034 1109 52-53 -0.08922657324327332 1109 54-55 -0.43755411759668306 1109 56-57 0.3478196684351005 1109 58-59 0.607556843179843 1109 60-61 1.2692989537523545 1109 62-63 0.5639274889242003 1109 64-65 1.4986000721857238 1109 66-67 0.5404138240208525 1109 68-69 0.3321204994661997 1109 70-71 1.0953508888998797 1109 72-73 0.6485694186676838 1109 74-75 -0.34570498230118574 1109 76 0.9493922297683071 1110 1 -0.8332271460218834 1110 2 -0.3968728263221024 1110 3 0.39060628730314306 1110 4 0.23659690823749457 1110 5 0.48622365111462784 1110 6 -0.41919554709988205 1110 7 0.664274834622212 1110 8 1.0002919400614587 1110 9 1.3825769369397207 1110 10-11 -0.38653751999793684 1110 12-13 0.32295158614502384 1110 14-15 0.764469216693584 1110 16-17 0.6943683571594335 1110 18-19 0.13097413541086667 1110 20-21 0.9623863099566705 1110 22-23 -0.806680123053745 1110 24-25 -1.090584272140667 1110 26-27 1.4144982511833675 1110 28-29 0.8934169054092536 1110 30-31 -0.009513918506264929 1110 32-33 0.8667760295856795 1110 34-35 0.7875326825492053 1110 36-37 1.3328800019077391 1110 38-39 0.8305988969674942 1110 40-41 -1.6320509528123495 1110 42-43 -1.0387530609215716 1110 44-45 0.6452488622491117 1110 46-47 0.5499612619260645 1110 48-49 1.3031973714378466 1110 50-51 -0.7170080692161349 1110 52-53 0.6603741264829281 1110 54-55 -0.5652921766170635 1110 56-57 0.6765959081673856 1110 58-59 -1.1705653046753426 1110 60-61 -0.009849966235478291 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-0.38735811733091197 2116 56-57 0.06252301040573371 2116 58-59 -2.7053127335924643 2116 60-61 -1.709373559701902 2116 62-63 0.6308997831239225 2116 64-65 -2.051695589232125 2116 66-67 -1.2645160809688534 2116 68-69 -2.0889146477001006 2116 70-71 1.0452213294021675 2116 72-73 0.3861948256173875 2116 74-75 -0.2048173336399728 2116 76 -1.2895952129265282 2117 1 0.7654570645044316 2117 2 0.12255073257514226 2117 3 -0.28483230918809 2117 4 -1.019355472714885 2117 5 -0.5711071759530455 2117 6 -0.001589030809157066 2117 7 -0.293118649087063 2117 8 0.04477815560030507 2117 9 -2.4134756946392244 2117 10-11 -2.5381665926796373 2117 12-13 -2.6974118223762815 2117 14-15 -1.0450545928302262 2117 16-17 -1.7830752518631243 2117 18-19 -2.0101599498021656 2117 20-21 -0.8552828629756632 2117 22-23 -1.1133843837053732 2117 24-25 -2.947883770887529 2117 26-27 -3.4017611473128753 2117 28-29 -3.354076828926587 2117 30-31 -1.9960427405613999 2117 32-33 -3.949640010514571 2117 34-35 -0.951659046774104 2117 36-37 -1.7596951860621814 2117 38-39 -0.8501655140600732 2117 40-41 -0.5568629828875373 2117 42-43 -1.3770989255832262 2117 44-45 -1.065277453540368 2117 46-47 -2.921999890956144 2117 48-49 -2.896520673674935 2117 50-51 -1.9922273674617443 2117 52-53 -0.8363363998328666 2117 54-55 -1.0903548332586652 2117 56-57 -0.7580845429604395 2117 58-59 -3.0103209437730882 2117 60-61 -2.7697183872881084 2117 62-63 -2.1221790346100704 2117 64-65 -2.3957021573766184 2117 66-67 -2.969770596568189 2117 68-69 -1.7137093931845016 2117 70-71 -1.7700496065584161 2117 72-73 -0.7427050101790016 2117 74-75 -1.0276826866777355 2117 76 -0.31299422770485563 2118 1 -0.3514260523786845 2118 2 -0.21078260075819344 2118 3 -1.8118885862443648 2118 4 -0.60593555929497 2118 5 -0.3600682149140795 2118 6 0.07525079603067297 2118 7 0.5337211777527671 2118 8 0.5404491512713037 2118 9 -3.267371798535329 2118 10-11 -0.8650064195194673 2118 12-13 -1.117325242289695 2118 14-15 0.5339497361741081 2118 16-17 -4.835023303811177 2118 18-19 -1.7466318112740211 2118 20-21 -1.2822309149237086 2118 22-23 0.7513342310132387 2118 24-25 0.21012488712112543 2118 26-27 -3.8725403680920962 2118 28-29 -1.7766958765456309 2118 30-31 -2.2211509656696293 2118 32-33 -0.7396833005578571 2118 34-35 -1.8152954104104637 2118 36-37 -4.228851030218024 2118 38-39 -1.2695378084323679 2118 40-41 -3.581213632238189 2118 42-43 -0.94203399051829 2118 44-45 -4.373719011981926 2118 46-47 -1.7867185056747559 2118 48-49 -0.8835336606879238 2118 50-51 -2.8872490124833945 2118 52-53 -3.501920815417286 2118 54-55 -1.9956578635616964 2118 56-57 -0.39120142607732333 2118 58-59 -2.520061203513343 2118 60-61 -2.66906903663876 2118 62-63 -3.5815946190256582 2118 64-65 -1.4995982612727232 2118 66-67 -1.4654415922391877 2118 68-69 -3.226696406171513 2118 70-71 -1.7505690870779063 2118 72-73 0.138247370773378 2118 74-75 -0.6299554139504693 2118 76 -0.8032539679645936 2119 1 0.8765681756155459 2119 2 -0.940941330916921 2119 3 -0.3919751663309441 2119 4 -1.74157769493711 2119 5 -1.2893611442070103 2119 6 -1.0214303006504295 2119 7 -2.2137535697219803 2119 8 -0.3599837491615929 2119 9 -0.8301423613058887 2119 10-11 -0.67308722760027 2119 12-13 -0.618046743011206 2119 14-15 -0.39029268806832107 2119 16-17 -1.314821283609156 2119 18-19 -0.4327789974212166 2119 20-21 -1.373140005832802 2119 22-23 -0.2681462884672854 2119 24-25 0.4410051180013568 2119 26-27 0.2688737733220421 2119 28-29 -0.9512990511488084 2119 30-31 0.10713186261320828 2119 32-33 0.35194729107273304 2119 34-35 0.5562774611624022 2119 36-37 -0.7021555035224978 2119 38-39 -3.427546466441026 2119 40-41 -1.5588471098716639 2119 42-43 -1.4802735287578273 2119 44-45 0.010119371856454507 2119 46-47 -0.8942221131783583 2119 48-49 -1.4044571816114413 2119 50-51 0.30539168015729956 2119 52-53 -0.8403046538011196 2119 54-55 -0.06852943643327336 2119 56-57 -1.7620527969286925 2119 58-59 -0.6888923723445188 2119 60-61 -0.5276548952246145 2119 62-63 -0.6320996695307031 2119 64-65 -1.078241839916302 2119 66-67 -0.2078658346634299 2119 68-69 -0.8089474884225965 2119 70-71 -0.5954464319552457 2119 72-73 0.20173943426544838 2119 74-75 -1.1645874485825018 2119 76 -1.3645815292921526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 6.0 23 15.0 24 17.0 25 61.0 26 109.0 27 212.0 28 351.0 29 600.0 30 989.0 31 1573.0 32 2620.0 33 3708.0 34 5756.0 35 8564.0 36 12972.0 37 14369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.81813454538451 21.150549852666447 10.457793270804846 27.573522331144197 2 20.44180806193787 19.16299135257978 34.761088534945976 25.634112050536373 3 18.512027425225817 23.378849450147335 29.801821928625078 28.30730119600177 4 13.593205323267146 17.56832232344048 39.59709569940104 29.241376653891336 5 13.017352618300176 37.23205515860023 36.101534965236986 13.649057257862603 6 29.68048841553839 39.12716907728752 17.78980413304316 13.402538374130923 7 29.543747472218477 29.799895999845926 22.47944071028253 18.176915817653065 8 25.14300021185217 34.55116229801822 19.211139572058624 21.094697918070988 9 24.077961596980142 14.914392465766616 22.5141074283073 38.49353850894594 10-11 25.127592781618937 25.276852262003352 28.090634208346977 21.504920748030738 12-13 24.659592088284576 23.853590894208732 27.100706815861948 24.386110201644744 14-15 21.669587658648386 25.263370760549275 24.8001848891628 28.266856691639543 16-17 22.453440671763957 28.422856922750995 27.06218824027887 22.06151416520617 18-19 22.359070161585425 28.013597057180828 27.37226277372263 22.255070007511122 20-21 21.609883866494616 28.37181981010342 26.94663251352965 23.071663809872312 22-23 22.363884983533307 27.59278161893573 27.870115363133873 22.173218034397088 24-25 22.30803304893785 28.260115940912506 27.353966450320666 22.077884559828977 26-27 21.432698418812475 28.73967220692179 27.711226238853687 22.116403135412053 28-29 21.721587735685535 27.650559482310342 28.993894805770083 21.63395797623404 30-31 22.06247712959575 27.37996648883924 28.006856306453788 22.550700075111223 32-33 21.581957899196887 28.39204206228454 27.55811490091096 22.46788513760761 34-35 22.484255532230417 27.329892340581246 27.920189511391868 22.265662615796465 36-37 22.88099686073609 27.457003640005397 27.569670473585887 22.09232902567263 38-39 21.891069468251064 28.242782581900123 28.230264044835625 21.63588390501319 40-41 22.022995589623097 27.108410530978567 28.568264545577104 22.300329333821235 42-43 21.7148469849585 27.44159620977216 28.762783352271633 22.080773452997708 44-45 21.34603162375055 27.32604048302294 28.71944995474067 22.608477938485834 46-47 21.461587350499777 26.82048417849508 29.23174700999557 22.486181461009572 48-49 21.092771989291837 27.406929491747395 29.000635556497123 22.49966296246365 50-51 20.777882633900198 27.131521676328408 28.789746355179783 23.300849334591607 52-53 20.693141767617433 27.189299539703022 29.331895306511562 22.78566338616798 54-55 20.29351154594303 27.330855304970825 29.358858309419716 23.01677483966643 56-57 20.38884502051114 26.849373110182384 29.16433950272519 23.597442366581284 58-59 20.44758584827533 27.00729927007299 28.786857462011056 23.75825741964062 60-61 20.452400670223213 26.963002908152454 29.170117289062652 23.414479132561677 62-63 19.16973210330682 27.290410800608594 29.394487991834062 24.145369104250523 64-65 20.12788167093581 27.99048591183098 27.717966989580727 24.163665427652486 66-67 19.812029351154596 27.482040714134392 27.68233730716638 25.023592627544634 68-69 19.713806983417754 27.430040637097242 28.04441191764728 24.811740461837722 70-71 19.6618069063806 27.49552221558847 27.776707817344914 25.065963060686013 72-73 20.034474125146854 26.707817344914588 27.183521753365557 26.074186776573004 74-75 20.18277064114169 27.004410376904264 27.32507751863336 25.487741463320685 76 20.21069660843942 27.569670473585887 27.207595863104984 25.01203705486971 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 1.0 16 4.0 17 6.0 18 8.0 19 9.0 20 11.5 21 15.0 22 17.5 23 32.0 24 54.0 25 64.0 26 107.5 27 172.5 28 238.0 29 282.0 30 354.5 31 488.5 32 703.0 33 856.0 34 974.5 35 1220.5 36 1479.5 37 1611.0 38 1859.0 39 2410.5 40 2938.0 41 3366.5 42 3571.0 43 3700.0 44 3945.0 45 4139.0 46 4217.0 47 4175.5 48 3953.5 49 3532.5 50 3292.0 51 3014.0 52 2470.5 53 2013.0 54 1821.0 55 1639.5 56 1211.0 57 804.5 58 645.0 59 471.0 60 228.0 61 113.5 62 68.0 63 39.5 64 11.5 65 7.5 66 2.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.093118656472086 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93574774522827 40.18257804826377 2 12.413479689575615 13.67794618954991 3 4.984968188491925 8.239123317219729 4 2.877018807243236 6.340157540974135 5 1.8073131510871845 4.978525894112436 6 1.2095364608823325 3.9982281455231785 7 0.936866391666084 3.6130423896924295 8 0.5593232189051248 2.465188837316796 9 0.4544501153604139 2.2533366716098837 >10 1.8178004614416556 14.049650443926584 >50 0.0 0.0 >100 0.00349577011815703 0.20222252181114342 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 105 0.20222252181114342 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0038518575583074938 0.0 2 0.0 0.0 0.0 0.0038518575583074938 0.0 3 0.0 0.0 0.0 0.0038518575583074938 0.0 4 0.0 0.0 0.0 0.0038518575583074938 0.0 5 0.0 0.0 0.0 0.0038518575583074938 0.0 6 0.0 0.0 0.0 0.0038518575583074938 0.0 7 0.0 0.0 0.0 0.00577778633746124 0.0 8 0.0 0.0 0.0 0.00577778633746124 0.0 9 0.0 0.0 0.0 0.009629643895768734 0.0 10 0.0 0.0 0.0 0.009629643895768734 0.0 11 0.0 0.0 0.0 0.009629643895768734 0.0 12 0.0 0.0 0.0 0.009629643895768734 0.0 13 0.0 0.0 0.0 0.009629643895768734 0.0 14 0.0 0.0 0.0 0.01155557267492248 0.0 15 0.0 0.0 0.0 0.01155557267492248 0.0 16 0.0 0.0 0.0 0.013481501454076228 0.0 17 0.0 0.0 0.0 0.013481501454076228 0.0 18 0.0 0.0 0.0 0.013481501454076228 0.0 19 0.0 0.0 0.0 0.015407430233229975 0.0 20 0.0 0.0 0.0 0.017333359012383722 0.0 21 0.0 0.0 0.0 0.017333359012383722 0.0 22 0.0 0.0 0.0 0.019259287791537467 0.0 23 0.0 0.0 0.0 0.019259287791537467 0.0 24 0.0 0.0 0.0 0.019259287791537467 0.0 25 0.0 0.0 0.0 0.021185216570691216 0.0 26 0.0 0.0 0.0 0.02503707412899871 0.0 27 0.0 0.0 0.0 0.028888931687306205 0.0 28 0.0 0.0 0.0 0.034666718024767444 0.0 29 0.0 0.0 0.0 0.038518575583074935 0.0 30 0.0 0.0 0.0 0.05777786337461241 0.0 31 0.0 0.0 0.0 0.07318529360784239 0.0 32 0.0 0.0 0.0 0.08281493750361112 0.0 33 0.0 0.0 0.0 0.09629643895768734 0.0 34 0.0 0.0 0.0 0.13288908576160854 0.0 35 0.0 0.0 0.0 0.19066694913622095 0.0 36 0.0 0.0 0.0 0.2638522427440633 0.0 37 0.0 0.0 0.0 0.4025191148431331 0.0 38 0.0 0.0 0.0 0.6586676424705814 0.0 39 0.0 0.0 0.0 0.9186680276563373 0.0 40 0.0 0.0 0.0 1.2518537064499355 0.0 41 0.0 0.0 0.0 1.5696319550103037 0.0 42 0.0 0.0 0.0 2.026077075669742 0.0 43 0.0 0.0 0.0 2.5730408489494057 0.0 44 0.0 0.0 0.0 3.0853379042043025 0.0 45 0.0 0.0 0.0 3.5880053155634304 0.0 46 0.0 0.0 0.0 4.098376442039173 0.0 47 0.0 0.0 0.0 4.668451360668683 0.0 48 0.0 0.0 0.0 5.132600196444735 0.0 49 0.0 0.0 0.0 5.662230610712016 0.0 50 0.0 0.0 0.0 6.195712882537603 0.0 51 0.0 0.0 0.0 6.773491516283728 0.0 52 0.0 0.0 0.0 7.524603740153689 0.0 53 0.0 0.0 0.0 8.22949367332396 0.0 54 0.0 0.0 0.0 8.899716888469465 0.0 55 0.0 0.0 0.0 9.577643818731584 0.0 56 0.0 0.0 0.0 10.199718814398244 0.0 57 0.0 0.0 0.0 10.75823816035283 0.0 58 0.0 0.0 0.0 11.347572366773877 0.0 59 0.0 0.0 0.0 11.900313926391002 0.0 60 0.0 0.0 0.0 12.449203628449819 0.0 61 0.0 0.0 0.0 13.105945342141247 0.0 62 0.0 0.0 0.0 13.689501762224833 0.0 63 0.0 0.0 0.0 14.30002118521657 0.0 64 0.0 0.0 0.0 14.918244323324924 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATCG 15 0.0022129216 70.0 48 CAAACGA 15 0.0022129216 70.0 49 GCGCCTT 15 0.0022129216 70.0 11 TAGGCCA 15 0.0022129216 70.0 5 AAGATAG 20 7.8576166E-5 70.0 5 GCTGTCG 15 0.0022129216 70.0 54 AAAGCGC 15 0.0022129216 70.0 8 GACTATC 15 0.0022129216 70.0 38 AAGCTAC 20 7.8576166E-5 70.0 6 CTCATAG 25 2.3697535E-4 56.000004 1 CCATTTA 25 2.3697535E-4 56.000004 13 CTGTCGC 20 0.0069148806 52.5 55 GCCTTCC 20 0.0069148806 52.5 13 GCCTTAA 20 0.0069148806 52.5 60 GGTACCG 20 0.0069148806 52.5 58 AAATCGC 20 0.0069148806 52.5 49 CTCCGGT 20 0.0069148806 52.5 7 ACTGACT 20 0.0069148806 52.5 35 AACCCGT 20 0.0069148806 52.5 10 CGTTGAA 20 0.0069148806 52.5 42 >>END_MODULE