FastQCFastQC Report
Wed 25 May 2016
SRR1780537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770237
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT401.8298555E-532.9718932
TCGTTGT1300.032.16197234
CATCGTT1300.032.12645332
CGTGCGA1350.029.35601810
GTACAAG1650.029.3235861
ATTCGCA703.578316E-828.29286239
ATATTCC407.019415E-427.50694839
CGGACGT407.019415E-427.50694836
CCACGAC1450.027.2782239
CGGTATG609.826472E-625.63980943
CGAGAAT1550.025.58812114
TTCGGTA701.1353004E-625.13286826
GATGTAT1600.024.7691424
ATTCCCG450.001404708624.42362431
CCCGTGC1800.024.4141088
CCAACGT1001.7407729E-924.20296922
CCGTGCG1650.023.9717719
AGGGGTA2350.023.42235610
CGCGGAG500.00255448422.03708619
TTAAACG803.564326E-622.03421614
Produced by FastQC (version 0.11.5)