FastQCFastQC Report
Wed 25 May 2016
SRR1780537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770237
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT24610.3195120462922451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA803.6379788E-1233.0092110
CGAGAAT803.6379788E-1233.0092114
TCTAGCA1000.032.998493
ATGGCGA300.00574684229.330098
CGCATGT300.00574684229.3300926
TATGCCG3950.027.2898943
ATGCCGT4100.026.29148144
TCGTTGT959.622454E-1025.47086334
CCGTGCG1251.8189894E-1224.6420739
CGTATGC4400.024.50047141
CTCGCTA901.5063051E-824.45126513
CCGATAG450.001397068424.44659
TTCGGTA901.5124897E-824.4417426
CGGTAAG901.5124897E-824.4417428
CGGTAAA450.001398654724.4417433
CTCGTAT4450.024.22518539
TCGTATG4450.024.22518540
CATCGTT1001.7444108E-924.1973232
CGTTCAT752.0634689E-623.46559342
ATTGGCG1700.023.2915421
Produced by FastQC (version 0.11.5)