FastQCFastQC Report
Wed 25 May 2016
SRR1780536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52156
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATCT207.864906E-569.9875329
ATCAACG150.002214545269.9875344
TGCCTAC207.864906E-569.9875335
GAGGCAT207.864906E-569.987539
TACGACA252.3719468E-455.9900339
CAAGCTC252.3719468E-455.9900324
GGCATAA252.3719468E-455.9900311
AGCTCCA252.3719468E-455.9900326
GCTCCAT252.3719468E-455.9900327
GCAAGGG200.006919938652.4906513
CATTGCA200.006919938652.4906564
CTAAAAT200.006919938652.4906553
CAAGGGA200.006919938652.4906514
TTGCATA200.006919938652.4906566
AGGTGTG200.006919938652.490657
AGGGAAA200.006919938652.4906516
TCTGCCT352.0620935E-549.99109633
ATCCCTA305.4824166E-447.247251
TCATTGC305.8327615E-446.65835663
AGGCATA305.8327615E-446.65835610
Produced by FastQC (version 0.11.5)