##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52156 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.598032824603113 34.0 30.0 34.0 12.0 34.0 2 31.078150164889944 34.0 32.0 34.0 23.0 34.0 3 31.16074852365979 34.0 33.0 34.0 23.0 34.0 4 31.458432395122326 34.0 34.0 34.0 23.0 34.0 5 31.6157297338753 34.0 34.0 34.0 23.0 34.0 6 34.23190045248869 38.0 35.0 38.0 23.0 38.0 7 33.46872843009433 37.0 34.0 38.0 21.0 38.0 8 32.968747603343814 37.0 34.0 38.0 21.0 38.0 9 33.56708719993865 37.0 34.0 38.0 21.0 38.0 10-11 33.89939795996625 37.5 34.0 38.0 21.5 38.0 12-13 34.12092568448501 38.0 35.0 38.0 22.0 38.0 14-15 33.87460694838562 38.0 34.0 38.0 21.5 38.0 16-17 33.735236597898606 38.0 34.0 38.0 21.0 38.0 18-19 33.70351445663011 38.0 34.0 38.0 21.0 38.0 20-21 33.78122363678196 38.0 34.0 38.0 21.0 38.0 22-23 33.69076424572437 37.5 34.0 38.0 21.0 38.0 24-25 34.114905284147554 38.0 35.0 38.0 21.5 38.0 26-27 34.11502991026919 38.0 35.0 38.0 22.0 38.0 28-29 34.24186095559475 38.0 36.0 38.0 23.0 38.0 30-31 34.162359076616305 38.0 35.5 38.0 22.0 38.0 32-33 33.82069177084132 38.0 34.0 38.0 21.5 38.0 34-35 33.895860495436764 38.0 34.0 38.0 21.5 38.0 36-37 33.83349950149551 38.0 34.0 38.0 21.5 38.0 38-39 34.22309034435156 38.0 35.5 38.0 23.0 38.0 40-41 33.82372114425953 38.0 34.0 38.0 21.0 38.0 42-43 34.107581102845316 38.0 35.0 38.0 22.5 38.0 44-45 33.21796150011504 37.0 34.0 38.0 16.0 38.0 46-47 33.92608712324565 37.0 34.0 38.0 22.0 38.0 48-49 33.744746529641844 37.0 34.0 38.0 21.5 38.0 50-51 33.597352174246495 37.0 34.0 38.0 21.5 38.0 52-53 33.631691464069334 37.0 34.0 38.0 21.0 38.0 54-55 33.41202354475036 37.0 34.0 38.0 21.0 38.0 56-57 33.39496893933584 37.0 34.0 38.0 21.0 38.0 58-59 33.3289554413682 37.0 33.5 38.0 21.0 38.0 60-61 33.3398554336989 37.0 33.5 38.0 21.0 38.0 62-63 33.36518329626505 37.0 33.5 38.0 20.5 38.0 64-65 33.47227548124856 37.0 34.0 38.0 21.0 38.0 66-67 33.363889101925 37.0 33.5 38.0 20.5 38.0 68-69 32.91350947158524 37.0 32.5 38.0 15.5 38.0 70-71 32.91105529565151 37.0 32.0 38.0 19.0 38.0 72-73 32.995935271109744 37.0 32.0 38.0 19.0 38.0 74-75 32.59825331697216 37.0 31.5 38.0 15.0 38.0 76 31.89606181455633 36.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.118620886977304 1101 2 -1.543066590184246 1101 3 -0.15783739539910258 1101 4 -2.127522200353841 1101 5 -0.7517618932320431 1101 6 -2.7553200450229482 1101 7 -1.0501235656655084 1101 8 -1.159496652970006 1101 9 -1.0697816767020285 1101 10-11 -1.36393943481972 1101 12-13 -1.6067828407579299 1101 14-15 -1.0918328518652771 1101 16-17 -1.1742928757360147 1101 18-19 -0.5126173583103792 1101 20-21 -0.773394753374312 1101 22-23 -0.19157829548822036 1101 24-25 -1.254295822476358 1101 26-27 -2.1634296482697764 1101 28-29 -1.0487239689439534 1101 30-31 -1.6593028771977956 1101 32-33 -0.8236583224438547 1101 34-35 -1.2896011550591666 1101 36-37 -0.3440984850637676 1101 38-39 -1.5470084293243573 1101 40-41 -0.37599964761466254 1101 42-43 -0.939700748205432 1101 44-45 0.05779571932228578 1101 46-47 0.365774459381484 1101 48-49 -1.8331486195460442 1101 50-51 -1.9657485874449918 1101 52-53 -0.8064649948465998 1101 54-55 -1.5374687253226966 1101 56-57 -1.208549910057755 1101 58-59 -0.8891357859843652 1101 60-61 -0.3571036446903051 1101 62-63 -2.5956854865347765 1101 64-65 -0.8683680742961002 1101 66-67 -1.782980594401888 1101 68-69 -0.7256166259965227 1101 70-71 -0.2699626677258564 1101 72-73 -1.0270323349434065 1101 74-75 -1.7151539720598734 1101 76 -2.1704348100628614 1102 1 0.9432473597985762 1102 2 -0.05365390283673932 1102 3 0.7101043961658995 1102 4 0.4883718393812586 1102 5 0.8214779115779969 1102 6 0.9309382331227454 1102 7 1.4794163437105823 1102 8 2.031336389902073 1102 9 1.4786672602097823 1102 10-11 -0.2600922755307664 1102 12-13 -3.1737051133267684 1102 14-15 -0.933241008017248 1102 16-17 -2.3139103382665063 1102 18-19 -0.11655165597507278 1102 20-21 0.28845170880763504 1102 22-23 -1.0052764481546532 1102 24-25 0.4763376735152747 1102 26-27 -5.486625883891946 1102 28-29 0.3334431624607177 1102 30-31 0.3037940206760297 1102 32-33 -5.976411895117259 1102 34-35 -0.4849218242818978 1102 36-37 0.6062326407640484 1102 38-39 0.6752379980022454 1102 40-41 0.5350102861601727 1102 42-43 0.8343276589050532 1102 44-45 -5.405976500963526 1102 46-47 0.33616919622359376 1102 48-49 0.9002950890632277 1102 50-51 -5.520057580125368 1102 52-53 -0.931007518373967 1102 54-55 0.29147864309835825 1102 56-57 -0.6318681394059666 1102 58-59 0.8048995921961861 1102 60-61 0.3886047903044627 1102 62-63 -3.8477985921464857 1102 64-65 -0.1819355193989196 1102 66-67 0.334438366908671 1102 68-69 -7.054062948757068 1102 70-71 -0.24268835611903228 1102 72-73 0.6534748104034023 1102 74-75 -6.021358224412609 1102 76 -1.9193891495539752 1103 1 -0.7318887563538752 1103 2 -1.2270507570412157 1103 3 -1.0870069165987708 1103 4 -0.19553620659575444 1103 5 -0.5560931713016188 1103 6 -1.886590502509442 1103 7 0.13534495835062899 1103 8 0.3126431049655949 1103 9 -0.6997961396692247 1103 10-11 -0.38089536083022324 1103 12-13 -1.399657926393921 1103 14-15 -0.7215047708726701 1103 16-17 -1.3760322378430345 1103 18-19 -0.6985616378262591 1103 20-21 -1.62273250171868 1103 22-23 -1.2759963513609378 1103 24-25 -0.20949868462144394 1103 26-27 -0.7835103364025358 1103 28-29 0.12113977468091974 1103 30-31 -0.1248962394570654 1103 32-33 -5.091051943514117 1103 34-35 -1.860526784959447 1103 36-37 -0.5949167845233063 1103 38-39 -0.05578136073943796 1103 40-41 0.13486660799924977 1103 42-43 -0.1724403386751021 1103 44-45 -5.573512798604181 1103 46-47 -0.7405473796989739 1103 48-49 -1.32671640518965 1103 50-51 -0.7149683484254297 1103 52-53 -0.5386527089365742 1103 54-55 -0.18114688624223874 1103 56-57 0.6396079646472685 1103 58-59 -0.3670226703567465 1103 60-61 0.11126812968740296 1103 62-63 0.10611096623221528 1103 64-65 -0.18291858049994403 1103 66-67 -0.622080084391996 1103 68-69 -3.6638255016911287 1103 70-71 -0.6185201770506694 1103 72-73 0.674421573863782 1103 74-75 -1.867395731974618 1103 76 -0.4022990104130173 1104 1 -2.2965682809983328 1104 2 -0.242733593589044 1104 3 0.23824147615400548 1104 4 -0.16134567474303552 1104 5 -0.6419104321627138 1104 6 -1.1559261736569155 1104 7 -0.028983894172263547 1104 8 1.220007219518486 1104 9 -0.21401784237718857 1104 10-11 -0.35600738109128116 1104 12-13 -0.46258409043054627 1104 14-15 -0.2372850163431508 1104 16-17 -0.5712884233096247 1104 18-19 0.3701603571084817 1104 20-21 0.30523359997624055 1104 22-23 -0.3426240544585468 1104 24-25 -0.1330326803354538 1104 26-27 -0.018071022161350925 1104 28-29 -0.7050827072984305 1104 30-31 -0.9041676207155831 1104 32-33 -0.8868341371630493 1104 34-35 -0.6139360972524557 1104 36-37 -0.5971571897444221 1104 38-39 -0.3022746395237732 1104 40-41 0.289600681640394 1104 42-43 -0.0048969917045198486 1104 44-45 -2.963830351692046 1104 46-47 -0.1878978570026959 1104 48-49 0.5090521512101205 1104 50-51 0.4544257680756445 1104 52-53 0.1952748188249629 1104 54-55 -0.3171297422718453 1104 56-57 0.3369097374896626 1104 58-59 -0.3166622573429123 1104 60-61 -0.09134242943981263 1104 62-63 0.3740829124624909 1104 64-65 -0.2933315943320167 1104 66-67 0.34436254175255954 1104 68-69 -3.3669718098929593 1104 70-71 -1.1383915972727614 1104 72-73 0.31118370127465056 1104 74-75 -0.9050929850429981 1104 76 -0.5030198364406822 1105 1 -0.934427596823749 1105 2 -0.11937108039602151 1105 3 -0.8131026599358933 1105 4 0.4499103009197185 1105 5 -0.025904968699620667 1105 6 1.0559382331227454 1105 7 0.9729096924670912 1105 8 0.17679619325661378 1105 9 0.26148970092695834 1105 10-11 0.9537958100678452 1105 12-13 -1.1471001393533768 1105 14-15 0.3245669734749441 1105 16-17 1.1780286438040548 1105 18-19 0.28277599006309373 1105 20-21 -0.2381171171032932 1105 22-23 -0.47259859968733053 1105 24-25 0.08496260121915356 1105 26-27 -0.039257462839316304 1105 28-29 -0.5280013141153361 1105 30-31 -0.2713216531238558 1105 32-33 0.477029400429295 1105 34-35 0.7551736066030088 1105 36-37 0.6446941792255885 1105 38-39 0.325107864977376 1105 40-41 0.33652127517116526 1105 42-43 0.8664632864354189 1105 44-45 1.495736917197263 1105 46-47 1.0196206132276373 1105 48-49 0.02666871543685545 1105 50-51 -0.5064297605764949 1105 52-53 -0.4292362633132356 1105 54-55 -0.43582037367434623 1105 56-57 -0.04742626549040807 1105 58-59 0.13446147767912464 1105 60-61 -0.06161932768281275 1105 62-63 0.4430125703752026 1105 64-65 0.07377587447037115 1105 66-67 0.42184438194627205 1105 68-69 1.5895533034118188 1105 70-71 1.4471612679411194 1105 72-73 0.42928452700259356 1105 74-75 -1.3955629670384013 1105 76 -0.5554504566679128 1106 1 -0.5936282687632755 1106 2 -0.8593444827898047 1106 3 -0.7911946316584384 1106 4 -0.8810549122110984 1106 5 -0.370903918794113 1106 6 -0.5650808751575127 1106 7 0.13421017542361824 1106 8 0.04457809288565073 1106 9 0.2817178803908078 1106 10-11 -0.03458523563804761 1106 12-13 -0.0432994814125891 1106 14-15 -0.08778341499009912 1106 16-17 -0.3738541867411982 1106 18-19 -0.4730509855091043 1106 20-21 -0.7962993806995726 1106 22-23 -0.6252898574741295 1106 24-25 -0.5228451960790963 1106 26-27 -0.053588673030404266 1106 28-29 -1.1663263648607725 1106 30-31 -1.0456444640708682 1106 32-33 0.37181137005202913 1106 34-35 -0.28089064761411464 1106 36-37 -0.48930876050983585 1106 38-39 -0.5935769533889683 1106 40-41 -0.698668057788872 1106 42-43 -0.6408860521241095 1106 44-45 -0.15214227367556532 1106 46-47 0.034564268298680645 1106 48-49 -0.0248641466055588 1106 50-51 -0.35128872326985316 1106 52-53 -1.00762920794822 1106 54-55 -0.3463859227749282 1106 56-57 -0.07839847799128563 1106 58-59 -0.47856085031135365 1106 60-61 -0.08585464650411723 1106 62-63 -0.1334380490690492 1106 64-65 -0.33635221400166415 1106 66-67 -0.03402458984626833 1106 68-69 0.5448098303243967 1106 70-71 -0.40133736718171775 1106 72-73 -0.17593350337467228 1106 74-75 -1.5830410940069761 1106 76 -1.6165899541131417 1107 1 -0.2947578742810997 1107 2 -0.2683602128105669 1107 3 0.4322983478960545 1107 4 0.4331232205967268 1107 5 0.3641138522584271 1107 6 0.16919790163102988 1107 7 0.036627742372985495 1107 8 1.0294463055752345 1107 9 -0.5121731003601511 1107 10-11 -0.2642540958273685 1107 12-13 -0.08283567453641894 1107 14-15 -0.49968474166075083 1107 16-17 0.6950130406227046 1107 18-19 0.6175461922366168 1107 20-21 0.25710175242496547 1107 22-23 -1.2991118654271148 1107 24-25 -0.7946895146208064 1107 26-27 -0.015776799855885315 1107 28-29 -1.1913641943290472 1107 30-31 -0.27686169319428444 1107 32-33 0.590395316281402 1107 34-35 0.013369830268842975 1107 36-37 -0.2197342100646864 1107 38-39 0.6847247674119572 1107 40-41 0.6223721646132105 1107 42-43 0.7209044254069497 1107 44-45 -0.5737033110827525 1107 46-47 -0.3054962588505603 1107 48-49 0.050847575251076194 1107 50-51 0.6439254091156883 1107 52-53 0.44262944004417903 1107 54-55 0.24429923047840418 1107 56-57 0.5327145881311139 1107 58-59 0.5545179405532679 1107 60-61 -0.17240495089937724 1107 62-63 -0.08262339586850231 1107 64-65 0.14341341924603768 1107 66-67 1.2166352264608662 1107 68-69 0.6129732536273451 1107 70-71 0.12711391198216404 1107 72-73 0.418341050589504 1107 74-75 -0.06759259486914004 1107 76 -1.011494412711869 1108 1 -0.38328643029796794 1108 2 0.2796145493588398 1108 3 -0.5147087461573641 1108 4 -0.020503900263712183 1108 5 0.2800967219174524 1108 6 0.30150036330026353 1108 7 0.8659781624586316 1108 8 0.7761199464265189 1108 9 0.11694194099458599 1108 10-11 0.24331398420140005 1108 12-13 -0.07778483926884405 1108 14-15 -0.027292705307814913 1108 16-17 -0.5766205531528357 1108 18-19 -0.020689774941132555 1108 20-21 0.375788208340488 1108 22-23 -0.24056140611167365 1108 24-25 -0.3548091654756931 1108 26-27 -0.9253521373955351 1108 28-29 0.08505870279082473 1108 30-31 0.26692855820328276 1108 32-33 -0.009656990103245278 1108 34-35 -0.0463902141915824 1108 36-37 0.3784219898764718 1108 38-39 0.4297147965073762 1108 40-41 -0.8255666369167471 1108 42-43 0.023268020163229153 1108 44-45 -0.3468243676717151 1108 46-47 0.20601114577201685 1108 48-49 0.2420110653945855 1108 50-51 -0.03136636243618085 1108 52-53 -0.5179936597629364 1108 54-55 0.039454351600383575 1108 56-57 0.3879076313819496 1108 58-59 -1.5466458765051527 1108 60-61 0.2716435632723915 1108 62-63 0.10087393977503467 1108 64-65 0.2800311576487289 1108 66-67 -0.018138711882983216 1108 68-69 0.4215482315605925 1108 70-71 0.8609397971042654 1108 72-73 0.5165626335114766 1108 74-75 1.0909120963597374 1108 76 0.30211505474375144 1109 1 -0.8964655588617134 1109 2 -1.043247204272145 1109 3 0.40567855884532733 1109 4 -0.008597857588437563 1109 5 0.7503854075907519 1109 6 0.13169580888032328 1109 7 -0.35399673443934176 1109 8 0.4834894999499184 1109 9 0.8399112793485415 1109 10-11 1.3784045346765623 1109 12-13 0.9180014257481943 1109 14-15 0.6868546857626541 1109 16-17 -0.12948051370510427 1109 18-19 0.1287633360504401 1109 20-21 0.4242371952510169 1109 22-23 0.7172115901228651 1109 24-25 0.5747228409793905 1109 26-27 1.1137728401909897 1109 28-29 0.7164375803235501 1109 30-31 0.7708195390173387 1109 32-33 0.7941622675621716 1109 34-35 0.2822631836925922 1109 36-37 0.7032023377337424 1109 38-39 0.3374788273483347 1109 40-41 0.524783013432895 1109 42-43 0.5701928901650248 1109 44-45 0.08260005406039284 1109 46-47 -0.013013419406391336 1109 48-49 -0.02849278972464475 1109 50-51 1.194436040289311 1109 52-53 1.226707792630826 1109 54-55 0.36253127467730195 1109 56-57 0.30465498659084744 1109 58-59 0.6678780610957062 1109 60-61 0.7572451411816559 1109 62-63 -0.1350593576967256 1109 64-65 0.4784711791656733 1109 66-67 0.46167122177310205 1109 68-69 0.9293301931887044 1109 70-71 0.09694481772466901 1109 72-73 -0.056612908894841496 1109 74-75 2.0357390642636304 1109 76 -0.45997926119671817 1110 1 0.5303857355392516 1110 2 1.0728274495508536 1110 3 -0.26379766029976537 1110 4 0.3029413758051014 1110 5 0.21512552398650087 1110 6 0.542693200010163 1110 7 1.1684194806921084 1110 8 1.8140132808048293 1110 9 -0.7651459151126261 1110 10-11 -0.8838069844886007 1110 12-13 0.3468595433480246 1110 14-15 0.2028691348896885 1110 16-17 0.8124620911514029 1110 18-19 0.28208462844457927 1110 20-21 0.4120357102018488 1110 22-23 0.5037859429223843 1110 24-25 0.619876362954102 1110 26-27 -0.2594561383360059 1110 28-29 0.5473025211222691 1110 30-31 0.5493063943253915 1110 32-33 1.7061430020594557 1110 34-35 0.9653657323580518 1110 36-37 0.1956366142739796 1110 38-39 0.9198789878941867 1110 40-41 0.6438679020542111 1110 42-43 0.5867893180197328 1110 44-45 1.7167361166732746 1110 46-47 0.22339035342122315 1110 48-49 0.8059242281360781 1110 50-51 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0.06357867105079862 2116 44-45 1.477879774340117 2116 46-47 0.2071206132276373 2116 48-49 0.554141242909381 2116 50-51 0.5533229866762568 2116 52-53 1.1044175828406182 2116 54-55 -0.027853340707309826 2116 56-57 0.5480407674766283 2116 58-59 0.26770323592088374 2116 60-61 -0.8844215254850099 2116 62-63 0.7417763066389398 2116 64-65 -0.38158126838676765 2116 66-67 -0.29106770596581555 2116 68-69 0.9995807759392861 2116 70-71 -1.8358057650259099 2116 72-73 0.9485152962333672 2116 74-75 -0.2533926373680728 2116 76 1.0654286642112112 2117 1 -1.198518344785466 2117 2 0.030457585561677547 2117 3 -0.19842701300218124 2117 4 -0.39334859072626926 2117 5 -0.3612470459771835 2117 6 -0.26126606795252627 2117 7 -3.699842286736505 2117 8 -2.830293491252423 2117 9 1.0041341532488346 2117 10-11 -0.4716443411787594 2117 12-13 -0.47251665831828404 2117 14-15 -0.67404462997537 2117 16-17 -0.40689986854381743 2117 18-19 -0.805254259257481 2117 20-21 -0.42111959849287217 2117 22-23 0.4972702411492591 2117 24-25 0.0654364284671729 2117 26-27 0.2967640425370277 2117 28-29 -0.3732984900576781 2117 30-31 -0.7309399917744486 2117 32-33 0.33951906130605636 2117 34-35 -0.5966224468059522 2117 36-37 -1.0711867140746634 2117 38-39 -0.27092781976798364 2117 40-41 -2.1766832622269234 2117 42-43 -1.0454473835397664 2117 44-45 0.11280533265276915 2117 46-47 0.2582475776610522 2117 48-49 -0.3712102872808529 2117 50-51 0.44057484771843036 2117 52-53 0.5480176655535942 2117 54-55 -0.704135391989368 2117 56-57 -0.06367345915695921 2117 58-59 0.21176857917977543 2117 60-61 0.9959548186010068 2117 62-63 -0.8093506577944751 2117 64-65 -0.16502833207577083 2117 66-67 -2.1234705182073412 2117 68-69 0.1086068305298582 2117 70-71 0.44562516963083 2117 72-73 0.34553762873957083 2117 74-75 -0.8280928027940462 2117 76 0.4938819313741547 2118 1 0.07656141416526197 2118 2 1.6278816668567266 2118 3 0.7253588785256468 2118 4 1.0158443668537842 2118 5 0.9631060203113684 2118 6 1.1493448265293367 2118 7 0.5278547474121424 2118 8 -6.086940070479649 2118 9 -0.5297190902818301 2118 10-11 0.3823672386392687 2118 12-13 0.8199328276795868 2118 14-15 -0.6507077517997857 2118 16-17 -0.1488944331190254 2118 18-19 0.04101774830485283 2118 20-21 -1.7573478863340668 2118 22-23 0.8900387629500344 2118 24-25 0.5986988649554164 2118 26-27 1.364588691006837 2118 28-29 0.7000206639066349 2118 30-31 0.1874695556673558 2118 32-33 0.9468096202095211 2118 34-35 1.6645142659436658 2118 36-37 0.6501886847200922 2118 38-39 1.0366463265158359 2118 40-41 0.41069709934699006 2118 42-43 1.0367929567650904 2118 44-45 0.4545281259884675 2118 46-47 0.04709314070017001 2118 48-49 0.6228225615907022 2118 50-51 1.3287350745883373 2118 52-53 -0.2094560435330166 2118 54-55 0.31417962632565377 2118 56-57 1.3591671411030006 2118 58-59 1.5492966425142924 2118 60-61 -0.35008086614435285 2118 62-63 0.2479576253202609 2118 64-65 -0.12952082882632965 2118 66-67 -0.5891446290427425 2118 68-69 1.9274653913239064 2118 70-71 0.535073355853207 2118 72-73 -0.9196165718985014 2118 74-75 1.0549864835110512 2118 76 2.164329763112306 2119 1 0.05562289322674019 2119 2 -0.3820678430927842 2119 3 0.6612068143735819 2119 4 0.9072907583001779 2119 5 0.44825600546134936 2119 6 1.2586409358254471 2119 7 -0.2627600432026469 2119 8 1.0092880257484467 2119 9 0.6187810582183175 2119 10-11 1.1775855338575667 2119 12-13 1.085896593643355 2119 14-15 0.46683011573808386 2119 16-17 -0.2407417749663665 2119 18-19 -0.4052251979380941 2119 20-21 0.06645668747051303 2119 22-23 1.0181943911056592 2119 24-25 -0.14395037769382668 2119 26-27 0.6837155101336521 2119 28-29 0.5541935180794937 2119 30-31 0.21791208610988377 2119 32-33 0.8767175501174478 2119 34-35 0.45408955551896213 2119 36-37 0.25488128941269395 2119 38-39 0.004718794588306707 2119 40-41 0.6921048807547692 2119 42-43 0.30045497042710423 2119 44-45 0.19955337101371384 2119 46-47 0.061720405327434946 2119 48-49 0.21786265663079263 2119 50-51 1.2725277683810319 2119 52-53 -0.35179343587041245 2119 54-55 0.21253127467730337 2119 56-57 0.6295116614475234 2119 58-59 1.2596471028647471 2119 60-61 1.0243622582987797 2119 62-63 0.37366300102563343 2119 64-65 0.07073162142611977 2119 66-67 0.5917285518304425 2119 68-69 0.019461233319745475 2119 70-71 1.0176245884044377 2119 72-73 -0.7318381341200677 2119 74-75 0.7485564270809917 2119 76 1.677694776477317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 16.0 13 88.0 14 249.0 15 472.0 16 628.0 17 680.0 18 715.0 19 689.0 20 603.0 21 510.0 22 481.0 23 378.0 24 380.0 25 436.0 26 477.0 27 615.0 28 709.0 29 917.0 30 1141.0 31 1431.0 32 1905.0 33 2713.0 34 3996.0 35 6281.0 36 11596.0 37 14048.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.973597359735976 22.208712099280103 13.737865014571632 28.07982552641229 2 19.66025001917325 21.765472812332234 34.82629035969016 23.747986808804356 3 16.772758647135515 25.295268042027764 31.361684178234526 26.57028913260219 4 14.245724365365442 19.054375335531866 38.13559322033898 28.564307078763708 5 11.62474116113199 38.19311296878595 35.0429480788404 15.13919779124166 6 24.547511312217193 37.85182912800062 18.991103612240202 18.60955594754199 7 24.32126696832579 30.102001687245956 22.944627655495054 22.6321036889332 8 21.121251629726206 36.975611626658484 19.89416366285758 22.008973080757727 9 22.292737173096096 18.596134672904363 21.7693074622287 37.34182069177084 10-11 23.206342510928753 26.35746606334842 27.065917631720225 23.370273794002607 12-13 22.708796686862488 24.466025001917323 26.12930439450878 26.695873916711406 14-15 21.005253470358156 25.359498427793543 24.22827670833653 29.40697139351177 16-17 21.718498351100546 27.79450111204847 25.449612700360458 25.037387836490527 18-19 21.373379860418744 26.987307308842702 26.252013191195644 25.38729963954291 20-21 20.288173939719304 27.74656798834266 26.602883656722142 25.36237441521589 22-23 22.16331773909042 27.534703581563 26.160940256154614 24.14103842319196 24-25 21.365710560625814 28.473234143722674 25.98262903596902 24.178426259682492 26-27 20.211480941790015 28.73111434925991 27.141651967175395 23.915752741774675 28-29 21.429940946391596 27.263402101388145 28.143454252626736 23.163202699593526 30-31 21.55648439297492 27.498274407546592 27.139734642227165 23.805506557251324 32-33 20.00536850985505 28.592108290513075 27.159866554183605 24.24265664544827 34-35 21.52197254390674 27.80408773678963 27.24998082675052 23.42395889255311 36-37 21.661937265127694 27.312293887568067 26.868433162052302 24.157335685251937 38-39 21.360917248255234 27.480059820538383 27.755195950609707 23.40382698059667 40-41 21.001418820461694 26.686287291970245 27.980481632026994 24.33181225554107 42-43 20.310223176623975 26.93458087276632 28.05333998005982 24.70185597054989 44-45 19.74365365442135 27.32667382467981 28.211519288288976 24.718153232609865 46-47 20.32556177620983 27.194378403251783 28.247948462305388 24.232111358232995 48-49 19.71872843009433 27.638239128767545 28.235485850141885 24.407546590996244 50-51 19.76378556637779 27.398573510238517 27.63919779124166 25.198443132142035 52-53 19.491525423728813 27.039075082444974 28.10510775366209 25.364291740164123 54-55 19.3122555410691 27.442671984047855 27.743692000920316 25.501380473962726 56-57 19.757074929058977 27.107140118107214 27.870235447503646 25.265549505330164 58-59 19.72639772988726 27.079338906357847 27.65549505330163 25.538768310453257 60-61 19.434964337755964 26.917324948232224 27.37077229848915 26.27693841552266 62-63 19.411956438377175 26.625891556100928 27.887491372037733 26.074660633484164 64-65 20.117532019326635 26.996893933583866 26.799409463915946 26.086164583173556 66-67 19.932510161822226 27.329549812102155 26.390060587468362 26.347879438607258 68-69 19.184753432011657 27.60660326712171 26.162857581102845 27.04578571976379 70-71 20.563310069790628 27.220262290052915 26.05740470895007 26.159022931206383 72-73 20.356238975381547 26.716964491141958 25.822532402791627 27.104264130684868 74-75 20.361990950226243 26.974844696679195 25.652849144873073 27.010315208221492 76 20.964030983971163 26.033438147097172 25.80719380320577 27.1953370657259 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 1.0 15 2.5 16 4.0 17 5.0 18 9.5 19 10.0 20 8.5 21 11.0 22 16.0 23 32.5 24 43.0 25 42.0 26 91.0 27 166.0 28 234.0 29 276.0 30 311.5 31 452.0 32 658.0 33 759.0 34 891.5 35 1196.0 36 1492.0 37 1616.0 38 1819.0 39 2366.0 40 2876.5 41 3198.0 42 3353.0 43 3391.0 44 3684.5 45 3945.0 46 3950.0 47 3893.0 48 3735.5 49 3372.5 50 3110.0 51 2922.0 52 2444.0 53 2037.5 54 1921.0 55 1754.0 56 1379.0 57 1053.0 58 935.0 59 760.0 60 512.0 61 373.5 62 308.0 63 273.0 64 206.0 65 157.5 66 113.0 67 85.0 68 77.0 69 58.5 70 43.0 71 38.0 72 30.5 73 16.5 74 8.5 75 7.0 76 7.0 77 6.5 78 4.0 79 2.0 80 2.5 81 2.5 82 2.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6576424572436537 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52156.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.68993020937188 #Duplication Level Percentage of deduplicated Percentage of total 1 76.58741258741259 47.24672137433853 2 10.868686868686869 13.409770687936193 3 4.823620823620824 8.927064958969245 4 2.533022533022533 6.250479331237058 5 1.6161616161616161 4.985044865403789 6 0.9728049728049729 3.600736252780121 7 0.777000777000777 3.355318659406396 8 0.48174048174048173 2.377482935807961 9 0.40404040404040403 2.243270189431705 >10 0.9355089355089355 7.60411074468901 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.003834649896464453 0.0 10 0.0 0.0 0.0 0.003834649896464453 0.0 11 0.0 0.0 0.0 0.003834649896464453 0.0 12 0.0 0.0 0.0 0.003834649896464453 0.0 13 0.0 0.0 0.0 0.003834649896464453 0.0 14 0.0 0.0 0.0 0.003834649896464453 0.0 15 0.0 0.0 0.0 0.003834649896464453 0.0 16 0.0 0.0 0.0 0.003834649896464453 0.0 17 0.0 0.0 0.0 0.005751974844696679 0.0 18 0.0 0.0 0.0 0.007669299792928906 0.0 19 0.0 0.0 0.0 0.007669299792928906 0.0 20 0.0 0.0 0.0 0.007669299792928906 0.0 21 0.0 0.0 0.0 0.009586624741161133 0.0 22 0.0 0.0 0.0 0.009586624741161133 0.0 23 0.0 0.0 0.0 0.009586624741161133 0.0 24 0.0 0.0 0.0 0.011503949689393358 0.0 25 0.0 0.0 0.0 0.013421274637625585 0.0 26 0.0 0.0 0.0 0.01725592453409004 0.0 27 0.0 0.0 0.0 0.02109057443055449 0.0 28 0.0 0.0 0.0 0.04601579875757343 0.0 29 0.0 0.0 0.0 0.07094102308459238 0.0 30 0.0 0.0 0.0 0.08244497277398574 0.0 31 0.0 0.0 0.0 0.10545287215277245 0.0 32 0.0 0.0 0.0 0.13613007132448807 0.0 33 0.0 0.0 0.0 0.17639389523736482 0.0 34 0.0 0.0 0.0 0.22240969399493826 0.0 35 0.0 0.0 0.0 0.2741774675972084 0.0 36 0.0 0.0 0.0 0.3700437150088197 0.0 37 0.0 0.0 0.0 0.5521895850908812 0.0 38 0.0 0.0 0.0 0.7861032287752128 0.0 39 0.0 0.0 0.0 1.100544520285298 0.0 40 0.0 0.0 0.0 1.4360763862259376 0.0 41 0.0 0.0 0.0 1.8425492752511696 0.0 42 0.0 0.0 0.0 2.3237978372574584 0.0 43 0.0 0.0 0.0 2.843392898228392 0.0 44 0.0 0.0 0.0 3.3514840095099316 0.0 45 0.0 0.0 0.0 3.938185443668993 0.0 46 0.0 0.0 0.0 4.457780504639927 0.0 47 0.0 0.0 0.0 4.992714165196718 0.0 48 0.0 0.0 0.0 5.521895850908812 0.0 49 0.0 0.0 0.0 6.064498811258532 0.0 50 0.0 0.0 0.0 6.499731574507248 0.0 51 0.0 0.0 0.0 7.050003834649896 0.0 52 0.0 0.0 0.0 7.600276094792545 0.0 53 0.0 0.0 0.0 8.263670526880896 0.0 54 0.0 0.0 0.0 8.848454636091725 0.0 55 0.0 0.0 0.0 9.469667919318967 0.0 56 0.0 0.0 0.0 10.021857504409848 0.0 57 0.0 0.0 0.0 10.501188741467905 0.0 58 0.0 0.0 0.0 11.009279852749444 0.0 59 0.0 0.0 0.0 11.473272490221643 0.0 60 0.0 0.0 0.0 12.054221949536007 0.0 61 0.0 0.0 0.0 12.50479331237058 0.0 62 0.0 0.0 0.0 13.049313597668533 0.0 63 0.0 0.0 0.0 13.597668532862949 0.0 64 0.0 0.0 0.0 14.16711404248792 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCATCT 20 7.864906E-5 69.98753 29 ATCAACG 15 0.0022145452 69.98753 44 TGCCTAC 20 7.864906E-5 69.98753 35 GAGGCAT 20 7.864906E-5 69.98753 9 TACGACA 25 2.3719468E-4 55.99003 39 CAAGCTC 25 2.3719468E-4 55.99003 24 GGCATAA 25 2.3719468E-4 55.99003 11 AGCTCCA 25 2.3719468E-4 55.99003 26 GCTCCAT 25 2.3719468E-4 55.99003 27 GCAAGGG 20 0.0069199386 52.49065 13 CATTGCA 20 0.0069199386 52.49065 64 CTAAAAT 20 0.0069199386 52.49065 53 CAAGGGA 20 0.0069199386 52.49065 14 TTGCATA 20 0.0069199386 52.49065 66 AGGTGTG 20 0.0069199386 52.49065 7 AGGGAAA 20 0.0069199386 52.49065 16 TCTGCCT 35 2.0620935E-5 49.991096 33 ATCCCTA 30 5.4824166E-4 47.24725 1 TCATTGC 30 5.8327615E-4 46.658356 63 AGGCATA 30 5.8327615E-4 46.658356 10 >>END_MODULE