FastQCFastQC Report
Wed 25 May 2016
SRR1780536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52156
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT10331.9805966715238896Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT6761.296111665004985No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC2710.5195950609709333No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC1280.24541759337372496No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT820.15722064575504258Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG810.15530332080681034No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC550.10545287215277246No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT540.10353554720454022No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTATC150.002212981270.039
ACACCAT150.002212981270.054
TGACGCA150.002212981270.050
CGTAGAC150.002212981270.03
ATGACGT150.002212981270.030
TATCGTG150.002212981270.042
ATTGGCT252.369861E-455.99999625
CGGAATA200.006915067352.569
TACCAGG200.006915067352.559
CGTCTGA200.006915067352.534
AAGCCCT200.006915067352.549
TTCGGAA200.006915067352.567
TGAGCAC200.006915067352.549
GGCTCAT200.006915067352.556
ATATTCT200.006915067352.547
TTTACAC552.215529E-950.9090962
ATCGTGT350.001244779940.043
TCTATAC350.001244779940.03
TTGGCTT400.002398388735.026
CTATACA400.002398388735.01