FastQCFastQC Report
Wed 25 May 2016
SRR1780535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574700
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA250.002359932535.1751431
GACTCTC300.005733038829.34326611
TGAGACA300.005774663329.2998644
GCGAAGT508.374092E-526.38135732
TCGGTGG1151.2732926E-1124.84983842
ACGAGTG450.00140593324.4186785
CACTACG651.8248556E-523.6693959
CGCAAAG752.0426396E-623.49098812
CAAAGGC904.030244E-722.0247214
ACTCTAT500.00257373722.0074511
CCGCAAG602.8636685E-422.0074511
TGGATGT703.2053333E-522.00553336
CGAGTCT904.0642408E-722.00553134
CCTTACG703.211807E-521.9997831
GTATGTA500.002578950721.9997831
GAACGGC500.002584172921.9921225
GTAACCT500.002593332721.9787258
ATTTACT602.8907807E-421.9768127
CGGTGGT1057.80783E-820.93211743
GTACAAG856.1047467E-620.7056791
Produced by FastQC (version 0.11.5)