Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780535_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 574700 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT | 14782 | 2.5721245867409084 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT | 9726 | 1.6923612319471026 | RNA PCR Primer, Index 32 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG | 2774 | 0.4826866191056203 | No Hit |
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC | 2113 | 0.3676700887419523 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC | 1501 | 0.261179745954411 | No Hit |
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC | 1180 | 0.20532451713937708 | No Hit |
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT | 886 | 0.15416739168261703 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC | 786 | 0.1367670088741952 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTCTT | 985 | 0.0 | 43.104893 | 44 |
GCCGTCT | 1085 | 0.0 | 39.13209 | 43 |
GATACTT | 30 | 1.2912926E-4 | 36.709934 | 1 |
CGGTATG | 25 | 0.0023522258 | 35.19861 | 43 |
CGTGCGA | 65 | 4.656613E-10 | 33.856598 | 10 |
CGAGAAT | 65 | 4.656613E-10 | 33.856598 | 14 |
GTCGCCC | 60 | 7.998096E-9 | 32.998695 | 37 |
CTTATAA | 55 | 1.3574754E-7 | 32.03776 | 1 |
CCGTGCG | 70 | 1.031367E-9 | 31.435535 | 9 |
CTACGAA | 35 | 3.218165E-4 | 31.424595 | 8 |
CTATACA | 225 | 0.0 | 29.367949 | 1 |
GCGCCAA | 45 | 4.0669704E-5 | 29.332172 | 35 |
TACATAC | 45 | 4.0669704E-5 | 29.332172 | 3 |
CCGTGCT | 30 | 0.005746091 | 29.32962 | 20 |
CTTGGCA | 30 | 0.005746091 | 29.32962 | 19 |
CTTTATA | 85 | 2.6375346E-10 | 28.46946 | 2 |
GATGTAT | 70 | 3.5883204E-8 | 28.282135 | 24 |
TGCCGTC | 1510 | 0.0 | 28.263783 | 42 |
CTGCTTA | 40 | 7.029236E-4 | 27.498913 | 39 |
TCCGTGC | 40 | 7.032812E-4 | 27.496523 | 22 |