FastQCFastQC Report
Wed 25 May 2016
SRR1780535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574700
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT147822.5721245867409084No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT97261.6923612319471026RNA PCR Primer, Index 32 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG27740.4826866191056203No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC21130.3676700887419523No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC15010.261179745954411No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC11800.20532451713937708No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT8860.15416739168261703No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC7860.1367670088741952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT9850.043.10489344
GCCGTCT10850.039.1320943
GATACTT301.2912926E-436.7099341
CGGTATG250.002352225835.1986143
CGTGCGA654.656613E-1033.85659810
CGAGAAT654.656613E-1033.85659814
GTCGCCC607.998096E-932.99869537
CTTATAA551.3574754E-732.037761
CCGTGCG701.031367E-931.4355359
CTACGAA353.218165E-431.4245958
CTATACA2250.029.3679491
GCGCCAA454.0669704E-529.33217235
TACATAC454.0669704E-529.3321723
CCGTGCT300.00574609129.3296220
CTTGGCA300.00574609129.3296219
CTTTATA852.6375346E-1028.469462
GATGTAT703.5883204E-828.28213524
TGCCGTC15100.028.26378342
CTGCTTA407.029236E-427.49891339
TCCGTGC407.032812E-427.49652322