FastQCFastQC Report
Wed 25 May 2016
SRR1780534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34069
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTG150.00220709369.99412547
CGCATCT150.00220709369.99412515
GTGGAGG150.00220709369.99412528
AACAGGT150.00220709369.99412545
TGCAAGG150.00220709369.99412533
GAGGCCG150.00220709369.99412531
TGGAGGC200.006896819552.49559429
ATCTACT200.006896819552.49559418
ACAGGTT200.006896819552.49559446
CTCGCAT200.006896819552.49559413
GCTACTA200.006896819552.49559447
TAGTGGA200.006896819552.49559426
GCATCTA200.006896819552.49559416
CTACTAT305.8013544E-446.6627520
TCTACTA450.00425231231.108519
CGCTGCC2802.2214974E-411.24905570
ACGCTGC3105.695335E-410.16043869
TACACAT6250.0065074046.15948369
Produced by FastQC (version 0.11.5)