##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780534_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34069 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92124805541695 34.0 32.0 34.0 21.0 34.0 2 32.16384396372069 34.0 34.0 34.0 27.0 34.0 3 32.23056150752884 34.0 34.0 34.0 27.0 34.0 4 32.40115647656227 34.0 34.0 34.0 31.0 34.0 5 32.2771434441868 34.0 34.0 34.0 27.0 34.0 6 34.94138366256714 38.0 36.0 38.0 27.0 38.0 7 33.96245854002172 37.0 34.0 38.0 24.0 38.0 8 33.52220493703954 37.0 34.0 38.0 21.0 38.0 9 34.363820481963074 37.0 34.0 38.0 25.0 38.0 10-11 34.85934427191876 38.0 36.0 38.0 26.5 38.0 12-13 35.117907775396986 38.0 36.5 38.0 29.0 38.0 14-15 34.958642754410164 38.0 36.0 38.0 27.0 38.0 16-17 34.81000322874167 38.0 36.0 38.0 26.5 38.0 18-19 34.79540051072823 38.0 36.0 38.0 26.5 38.0 20-21 34.84355278992633 38.0 36.0 38.0 27.0 38.0 22-23 34.831430332560394 38.0 36.0 38.0 26.5 38.0 24-25 35.266018961519265 38.0 36.5 38.0 29.0 38.0 26-27 35.25300126214447 38.0 36.5 38.0 27.0 38.0 28-29 35.51872670169362 38.0 37.0 38.0 31.0 38.0 30-31 35.43238721418298 38.0 37.0 38.0 31.0 38.0 32-33 34.95676421380141 38.0 36.0 38.0 26.5 38.0 34-35 35.043632040858256 38.0 36.0 38.0 27.0 38.0 36-37 35.074994863365525 38.0 36.0 38.0 27.0 38.0 38-39 35.516231764947605 38.0 37.0 38.0 31.0 38.0 40-41 35.02464116939153 38.0 36.0 38.0 27.0 38.0 42-43 35.295077636561096 38.0 36.5 38.0 28.5 38.0 44-45 34.31490504564267 37.0 34.0 38.0 25.0 38.0 46-47 35.0452023833984 38.0 36.0 38.0 27.0 38.0 48-49 34.87584020663947 38.0 36.0 38.0 27.0 38.0 50-51 34.686577240306434 37.5 35.5 38.0 26.0 38.0 52-53 34.72257770994159 37.5 35.5 38.0 26.5 38.0 54-55 34.44823740056943 37.0 34.5 38.0 25.5 38.0 56-57 34.560597610731165 37.0 35.0 38.0 25.5 38.0 58-59 34.47148434060289 37.0 35.0 38.0 25.5 38.0 60-61 34.52469987378555 37.0 35.0 38.0 25.5 38.0 62-63 34.52723883882709 37.0 35.0 38.0 26.0 38.0 64-65 34.613945228800375 37.0 35.5 38.0 26.0 38.0 66-67 34.544791452640226 37.0 35.0 38.0 25.5 38.0 68-69 33.98392967213596 37.0 34.0 38.0 23.5 38.0 70-71 34.02492001526314 37.0 34.0 38.0 24.0 38.0 72-73 34.17579030790455 37.0 34.0 38.0 24.0 38.0 74-75 33.689365699022574 37.0 33.5 38.0 23.0 38.0 76 33.00645748334263 37.0 32.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.9020864619325053 1101 2 -2.3243365355881416 1101 3 -0.5056686205647196 1101 4 -2.0316247058009047 1101 5 -1.1510320986687432 1101 6 -2.200636583279014 1101 7 -0.9910889216270533 1101 8 -1.546634621532231 1101 9 -1.6631818203161828 1101 10-11 -1.6811787787575412 1101 12-13 -1.6778075479752488 1101 14-15 -1.349031206115157 1101 16-17 -1.7420145810333025 1101 18-19 -0.04891938318392164 1101 20-21 -1.231702846958811 1101 22-23 -0.9100428600966666 1101 24-25 -1.890278163268647 1101 26-27 -1.8590890704498193 1101 28-29 -0.3950236228662405 1101 30-31 -1.4040074134925646 1101 32-33 -0.6577506551405179 1101 34-35 -1.2782976390000442 1101 36-37 -0.39515680079720994 1101 38-39 -0.9469328968347739 1101 40-41 -0.5789157134758796 1101 42-43 -1.107045492695768 1101 44-45 0.23905889439429018 1101 46-47 -0.34532689296419505 1101 48-49 -2.2018089209800635 1101 50-51 -1.0307424414322597 1101 52-53 -1.3288788677648853 1101 54-55 -0.9828536493283266 1101 56-57 -0.930879163657643 1101 58-59 -0.2330643005991888 1101 60-61 -0.28548090201365994 1101 62-63 -1.2589269617464467 1101 64-65 -0.9526806939556849 1101 66-67 -0.5785303001507884 1101 68-69 0.3028821735960534 1101 70-71 0.4261729403128598 1101 72-73 -0.8520627598478825 1101 74-75 -1.9060302245961935 1101 76 -1.628656866556284 1102 1 0.6177038395091721 1102 2 0.18828906511085108 1102 3 0.36720162845231386 1102 4 0.13613414085702402 1102 5 0.5745249131268011 1102 6 1.0042563830818452 1102 7 1.5558748179972355 1102 8 2.5171486899575015 1102 9 1.4634236842709782 1102 10-11 0.11405931648054946 1102 12-13 -4.054129085764679 1102 14-15 -0.8093195416328527 1102 16-17 -2.1874492686698304 1102 18-19 -0.540706189649633 1102 20-21 0.5530940075628124 1102 22-23 -0.8142805184627662 1102 24-25 0.23759427010837442 1102 26-27 -4.759438568047017 1102 28-29 0.41270901147189676 1102 30-31 0.3481114156904823 1102 32-33 -6.35801277833842 1102 34-35 -0.47790445420319116 1102 36-37 -0.16007597947785257 1102 38-39 0.2545961551529956 1102 40-41 0.3990222507006109 1102 42-43 0.15062161084289727 1102 44-45 -6.7857554350071965 1102 46-47 0.2606145661882664 1102 48-49 0.7049189077661069 1102 50-51 -8.145901899710978 1102 52-53 -2.455921244348545 1102 54-55 1.158998687936851 1102 56-57 -1.0375633052041735 1102 58-59 0.7332965556699165 1102 60-61 1.0856418590173575 1102 62-63 -5.129831286779215 1102 64-65 -0.717774621434927 1102 66-67 1.1542787867867403 1102 68-69 -8.038358542874022 1102 70-71 -0.09396685872602006 1102 72-73 -0.024845634465613387 1102 74-75 -6.939756742726381 1102 76 -1.218521436237367 1103 1 0.07777302284501175 1103 2 -0.8593872771962623 1103 3 -1.3019215479651898 1103 4 -0.6815076097821695 1103 5 -0.07632483871557838 1103 6 -2.8836966925686305 1103 7 0.6759680651020616 1103 8 1.470149141153172 1103 9 0.922064081323164 1103 10-11 0.2656986607428422 1103 12-13 -1.834208796999448 1103 14-15 -1.7566424473329576 1103 16-17 -1.8473229338826371 1103 18-19 -0.4111754331448978 1103 20-21 -0.42385741370354424 1103 22-23 -0.18576495221220313 1103 24-25 0.2520325315010581 1103 26-27 -1.1825473062031264 1103 28-29 0.04396935137263114 1103 30-31 -0.47024472809053464 1103 32-33 -5.705057446709603 1103 34-35 -1.1786489270562583 1103 36-37 -0.1551880264022003 1103 38-39 -1.0802662301681067 1103 40-41 -0.10428651753521478 1103 42-43 0.07012078365083596 1103 44-45 -5.805984040812582 1103 46-47 -1.046536885157792 1103 48-49 -0.5078198499418178 1103 50-51 -0.06228030247830674 1103 52-53 -0.31537379360407414 1103 54-55 -0.4350004096718081 1103 56-57 0.8054206021503134 1103 58-59 0.22321985240627384 1103 60-61 0.6493356475569954 1103 62-63 -0.11197145823356891 1103 64-65 0.4054769953495452 1103 66-67 -0.302105631922835 1103 68-69 -2.899615874803633 1103 70-71 0.13760931814267963 1103 72-73 0.5277186609171451 1103 74-75 -1.7021270239716841 1103 76 -1.1082040952838383 1104 1 -1.235734631944311 1104 2 -0.08875999092068554 1104 3 0.4164093015131982 1104 4 0.5976551052970862 1104 5 0.446842753751568 1104 6 0.03352319633531664 1104 7 1.078962239333201 1104 8 1.699725079372925 1104 9 -1.0086363657707267 1104 10-11 -0.5388332455029214 1104 12-13 -0.4800901138941782 1104 14-15 -0.13155777045990646 1104 16-17 -0.24787841416986822 1104 18-19 0.679022372030559 1104 20-21 1.1085568933009284 1104 22-23 0.6705553295924318 1104 24-25 1.1820555636105325 1104 26-27 1.0454367068032369 1104 28-29 -0.6580696677304658 1104 30-31 -0.8633147727999244 1104 32-33 -0.9289430990602341 1104 34-35 -0.2909304607237715 1104 36-37 0.03868814213864624 1104 38-39 0.11394864586495146 1104 40-41 0.349852488019593 1104 42-43 0.6395896903030476 1104 44-45 -3.267710094192882 1104 46-47 0.38765224910585516 1104 48-49 0.3794582966507889 1104 50-51 0.6493441386543211 1104 52-53 -0.46433734867791543 1104 54-55 -1.294030351414122 1104 56-57 0.15577963209205592 1104 58-59 0.7118392806680234 1104 60-61 0.28732507988323164 1104 62-63 0.4831037348296974 1104 64-65 0.05078250950752761 1104 66-67 0.8140710379051015 1104 68-69 -4.525886027899425 1104 70-71 -0.4687109636619553 1104 72-73 0.9220419233477131 1104 74-75 -0.7383009841396841 1104 76 0.9648889815350188 1105 1 -1.2532874733105075 1105 2 -0.485819889991852 1105 3 -0.1668569897176937 1105 4 -0.02656781578826184 1105 5 -0.33535573962954857 1105 6 0.3008383429746786 1105 7 0.8074782928693622 1105 8 0.47022167850990826 1105 9 1.1385880911882467 1105 10-11 0.4901655111708152 1105 12-13 -1.0011535235336169 1105 14-15 -0.2989679949900008 1105 16-17 0.4605981454732273 1105 18-19 0.25453615832471854 1105 20-21 -0.31501510991709125 1105 22-23 0.08001192287846948 1105 24-25 0.16614830112241918 1105 26-27 0.4260773855131035 1105 28-29 -0.40753942564753487 1105 30-31 -0.4397427695819047 1105 32-33 0.39838082399981545 1105 34-35 0.5844077971567145 1105 36-37 1.0052646067038467 1105 38-39 0.4153089910708303 1105 40-41 0.6971061997141703 1105 42-43 0.3148255566089091 1105 44-45 1.8814524890002744 1105 46-47 0.8767548600910118 1105 48-49 0.1270996335922021 1105 50-51 -0.5228199804124429 1105 52-53 -0.9810491163953117 1105 54-55 -1.3021119721264753 1105 56-57 -0.397756534074837 1105 58-59 0.2197380592828111 1105 60-61 0.26677362811697947 1105 62-63 0.06092133155316759 1105 64-65 0.1730673043586819 1105 66-67 0.525473071109225 1105 68-69 0.5206655701405083 1105 70-71 0.38731916871572736 1105 72-73 0.9827454997391385 1105 74-75 -0.7941043923163775 1105 76 -1.1029088682419115 1106 1 0.09957789312990784 1106 2 -0.027619013627898426 1106 3 -1.394711616403832 1106 4 -0.4407786586441773 1106 5 0.1058333659637114 1106 6 -0.36244888101364126 1106 7 0.797168128765918 1106 8 1.4972858592814546 1106 9 1.3814783738585774 1106 10-11 0.2305641708494761 1106 12-13 -0.00781217118375821 1106 14-15 -0.16858737809851476 1106 16-17 0.6684338701918477 1106 18-19 -0.41248850015110605 1106 20-21 -0.33627982338045115 1106 22-23 -0.24226662338839589 1106 24-25 -0.29046309160891326 1106 26-27 -0.8414284139542048 1106 28-29 -0.6840666187053515 1106 30-31 -0.6320702891282579 1106 32-33 -0.34790322102124094 1106 34-35 0.5579020836074449 1106 36-37 0.5459897549124548 1106 38-39 0.3469182358513123 1106 40-41 0.10508798509091832 1106 42-43 0.24690735057746593 1106 44-45 0.6017033696601217 1106 46-47 0.6156069951541525 1106 48-49 0.6072525676930738 1106 50-51 0.8991234855210521 1106 52-53 4.785062526835304E-4 1106 54-55 -0.7350959054540809 1106 56-57 0.7857643869664841 1106 58-59 -0.20070184105227185 1106 60-61 0.5836822972466251 1106 62-63 -0.13548729461746234 1106 64-65 0.49826706378819097 1106 66-67 -0.019676855860446096 1106 68-69 0.7188784750292498 1106 70-71 -0.4588016320403554 1106 72-73 -0.03491065628800527 1106 74-75 -1.1891555135467229 1106 76 -0.23401978842405669 1107 1 -0.1281516248397736 1107 2 0.2280444167928124 1107 3 -0.10591924713113343 1107 4 0.039803865627668245 1107 5 0.35723857802298653 1107 6 0.6151528904051986 1107 7 -0.07755508704058656 1107 8 1.0598816691945885 1107 9 -1.2701993641758307 1107 10-11 -0.3815547186071697 1107 12-13 0.26041300340320106 1107 14-15 -0.4269760682705481 1107 16-17 0.7339252685907596 1107 18-19 0.6047147020291064 1107 20-21 0.908397403667756 1107 22-23 0.11176165118152426 1107 24-25 -0.12285961690272984 1107 26-27 0.3498081726579514 1107 28-29 -0.768081266976516 1107 30-31 0.47719559402622735 1107 32-33 0.7710713999973251 1107 34-35 0.27583769934581426 1107 36-37 -0.22823950756412614 1107 38-39 0.5162249979020714 1107 40-41 0.5369990734915362 1107 42-43 0.2652602717152561 1107 44-45 -0.06871052916461196 1107 46-47 0.5262269917475777 1107 48-49 -0.3465457630853308 1107 50-51 0.6294079345075971 1107 52-53 0.4460584755935173 1107 54-55 0.3365699095689152 1107 56-57 0.9865393827082656 1107 58-59 1.0871111379972973 1107 60-61 -0.5517716288307 1107 62-63 0.1646319354966579 1107 64-65 0.19631679977865701 1107 66-67 0.9606927574933266 1107 68-69 0.6904761585584609 1107 70-71 0.42341604808228084 1107 72-73 0.9724986436608987 1107 74-75 -0.18911293136311258 1107 76 -0.7331681447517724 1108 1 -1.1294674143134564 1108 2 -0.23862224987385616 1108 3 0.18837899848289652 1108 4 -0.1185294677056632 1108 5 0.03706313942649331 1108 6 0.3160300833876519 1108 7 0.6220063164681875 1108 8 0.35812728322967047 1108 9 -0.77151515364951 1108 10-11 -0.3359406835194534 1108 12-13 0.19659721392950757 1108 14-15 0.3331116510276999 1108 16-17 -0.33010981912854476 1108 18-19 0.06655680729226532 1108 20-21 -0.21682189457786194 1108 22-23 -0.8588523839061963 1108 24-25 -1.5390876870781796 1108 26-27 -0.7977795466402924 1108 28-29 -0.34264267048529007 1108 30-31 -0.3212693182544726 1108 32-33 0.7017731543832966 1108 34-35 -0.25508335328576237 1108 36-37 0.04412891348850678 1108 38-39 0.5697337698318918 1108 40-41 -0.06343952299969402 1108 42-43 0.6352164120661357 1108 44-45 -0.20379824846285288 1108 46-47 0.07907786894055846 1108 48-49 -0.22680892098006922 1108 50-51 0.09782898713917376 1108 52-53 0.27588303699701555 1108 54-55 0.12428920781452035 1108 56-57 0.9661446458661587 1108 58-59 -0.6205643005991917 1108 60-61 0.9192810027482494 1108 62-63 -0.8369031522226393 1108 64-65 -0.5806568844318747 1108 66-67 -0.7303160144365037 1108 68-69 1.2665726497865322 1108 70-71 1.3023634165033329 1108 72-73 0.16877057348546032 1108 74-75 0.8874221563561875 1108 76 0.5558669429675263 1109 1 -0.4605280203740634 1109 2 -0.11437982563143123 1109 3 0.0982274833313852 1109 4 -0.5238324980086944 1109 5 0.06585101821437434 1109 6 -0.06730324994568093 1109 7 -3.0772361077742403 1109 8 -1.3267212016188132 1109 9 -0.6631818203161828 1109 10-11 0.36860477102599987 1109 12-13 -0.2825694527371567 1109 14-15 -0.7691610762450267 1109 16-17 -0.4755643645830858 1109 18-19 -0.716018950283491 1109 20-21 -0.09598856124452482 1109 22-23 0.326753676699866 1109 24-25 0.0919729189824352 1109 26-27 -0.004976516337258374 1109 28-29 -0.15021842806104502 1109 30-31 -0.12240568189083945 1109 32-33 0.8760155786257116 1109 34-35 0.12559846489605775 1109 36-37 0.48579558015517676 1109 38-39 0.19700649710462415 1109 40-41 -0.21874255330272518 1109 42-43 0.3189285332782603 1109 44-45 -0.43258612725072965 1109 46-47 -0.9379675856048948 1109 48-49 -0.591202860374004 1109 50-51 0.7357077750179641 1109 52-53 0.5955800066939929 1109 54-55 -0.3469229133975986 1109 56-57 0.011977979199500055 1109 58-59 -0.2679127854476846 1109 60-61 -0.024658391191145768 1109 62-63 -0.7297061825256677 1109 64-65 -0.6128538541288435 1109 66-67 -1.0685735901940774 1109 68-69 0.5790726497865322 1109 70-71 -0.6021820380421161 1109 72-73 -1.0808506386357593 1109 74-75 0.9749221563561861 1109 76 -1.4433754812748987 1110 1 0.39833786621459666 1110 2 0.5283744497961109 1110 3 0.29489715029807684 1110 4 0.8566355487959854 1110 5 0.5213865717697317 1110 6 0.2812116015394679 1110 7 1.250306646501187 1110 8 2.592450715572909 1110 9 -0.8097164737815277 1110 10-11 -0.37603969342043797 1110 12-13 0.43624242845096717 1110 14-15 0.12791363122572363 1110 16-17 1.1990981016635374 1110 18-19 1.273900041615697 1110 20-21 0.3272787654881455 1110 22-23 1.1351245137835804 1110 24-25 0.9766713888294163 1110 26-27 -0.045840602745904846 1110 28-29 0.8471263064123988 1110 30-31 0.47736929560691266 1110 32-33 1.59797777484534 1110 34-35 1.1351394189914714 1110 36-37 0.38084508510566906 1110 38-39 0.840525849039814 1110 40-41 0.4201413350861145 1110 42-43 0.4614127817030962 1110 44-45 2.028214952857276 1110 46-47 0.9363798491385822 1110 48-49 1.0176630262146489 1110 50-51 1.1479940036408252 1110 52-53 0.5706025089442193 1110 54-55 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0.8580869123532651 2116 50-51 0.12022482047250804 2116 52-53 0.17015387033035267 2116 54-55 -0.14862745885213968 2116 56-57 0.5291654791994986 2116 58-59 -1.0950434672658602 2116 60-61 -0.8833231639184191 2116 62-63 0.3683051811106921 2116 64-65 0.3469472822347939 2116 66-67 -1.3672951811031666 2116 68-69 0.3400101497865293 2116 70-71 -1.4820115834966643 2116 72-73 0.7521039068187889 2116 74-75 -1.450077843643811 2116 76 -2.7180913903658066 2117 1 1.1394719796259345 2117 2 0.7674383561867515 2117 3 1.4891365742404759 2117 4 1.1034402292640308 2117 5 -0.2523307999674458 2117 6 1.0599694773270514 2117 7 -0.08632701686514821 2117 8 -0.6539939288915377 2117 9 1.53681817968382 2117 10-11 0.7686047710260056 2117 12-13 1.253794183626482 2117 14-15 0.7990207419367863 2117 16-17 1.0562538172350955 2117 18-19 1.120344686080145 2117 20-21 0.2540114387554695 2117 22-23 0.835844585790781 2117 24-25 0.9919729189824338 2117 26-27 1.3041143927536538 2117 28-29 0.8906906628480442 2117 30-31 1.0003215908364353 2117 32-33 1.8487428513529949 2117 34-35 0.8892348285324232 2117 36-37 0.5857955801551782 2117 38-39 0.6651883152864428 2117 40-41 0.16307562851545754 2117 42-43 0.32801944236916825 2117 44-45 1.4901411454765352 2117 46-47 1.0438505962132894 2117 48-49 1.2406153214441744 2117 50-51 0.9129805022906865 2117 52-53 0.981943643057626 2117 54-55 -0.7469229133975901 2117 56-57 -0.39711292989141356 2117 58-59 -0.10882187635677099 2117 60-61 1.5344325178997593 2117 62-63 1.1021119992925108 2117 64-65 1.1053279640529752 2117 66-67 -0.1049372265577162 2117 68-69 0.7881635588774358 2117 70-71 2.302363416503333 2117 72-73 2.2600584522733342 2117 74-75 0.5476494290834637 2117 76 2.3384427005432826 2118 1 -1.9507637106097526 2118 2 0.6539703427187362 2118 3 0.039304924408824604 2118 4 0.20647053229433254 2118 5 0.4907668431302028 2118 6 1.4289930463506195 2118 7 1.580339649801516 2118 8 -2.5677986426962534 2118 9 -1.1854040425384014 2118 10-11 0.7807259831472209 2118 12-13 1.521134250966547 2118 14-15 -0.3918883489723015 2118 16-17 0.4069272179084962 2118 18-19 0.7304456961811567 2118 20-21 -1.9108033760593415 2118 22-23 -0.1245931246469354 2118 24-25 -0.30853213152261816 2118 26-27 0.5554611941004524 2118 28-29 -0.7037537815963972 2118 30-31 0.10974920026404789 2118 32-33 0.551773154383298 2118 34-35 1.5000092393068343 2118 36-37 -0.3364266420670461 2118 38-39 0.2252893253874504 2118 40-41 0.9263752918151198 2118 42-43 0.6180867824365066 2118 44-45 0.5165721219075223 2118 46-47 -0.1852739829112835 2118 48-49 0.2333762642051127 2118 50-51 0.8589400982502866 2118 52-53 -0.040783629669647325 2118 54-55 0.7668818004071127 2118 56-57 1.0138298310513463 2118 58-59 1.2021208845859874 2118 60-61 -0.7510893676221286 2118 62-63 1.328374625555135 2118 64-65 0.2031394118644272 2118 66-67 -1.1557789773994642 2118 68-69 1.3512948720087508 2118 70-71 -0.9235625094225881 2118 72-73 -1.3242849820700968 2118 74-75 0.7489962304302651 2118 76 0.825311387411972 2119 1 0.24624180554469888 2119 2 0.02237065792755999 2119 3 0.14174779280914152 2119 4 -0.034664219478713676 2119 5 0.6629496642491901 2119 6 -0.01623942015844193 2119 7 -2.0224972296311066 2119 8 0.4361414676268325 2119 9 0.6219245626625423 2119 10-11 0.2204422952039522 2119 12-13 0.8174305472628447 2119 14-15 -0.4769947319510237 2119 16-17 -0.422663010617903 2119 18-19 -0.7293651785233379 2119 20-21 0.5157135664150516 2119 22-23 1.4441618004909742 2119 24-25 1.623500965404098 2119 26-27 0.9228764817285011 2119 28-29 0.7247332160395317 2119 30-31 0.3997413200434039 2119 32-33 1.5021277643123767 2119 34-35 1.481884731820621 2119 36-37 0.02409345249559891 2119 38-39 1.068669940044657 2119 40-41 0.47680870395065966 2119 42-43 -0.4100850063735777 2119 44-45 -0.27755711371108305 2119 46-47 0.9910459540469461 2119 48-49 0.47194994426816095 2119 50-51 1.1183963630257026 2119 52-53 -0.24823043818028623 2119 54-55 -1.0668455439585216 2119 56-57 1.656658830263332 2119 58-59 0.7279286072022231 2119 60-61 1.1228271020390252 2119 62-63 0.6818025215362198 2119 64-65 0.8447863779794744 2119 66-67 0.5660492337904515 2119 68-69 -0.16348054170283177 2119 70-71 0.07683150160971763 2119 72-73 -0.02582162509610697 2119 74-75 0.983432794654064 2119 76 1.8560442479320685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 7.0 14 36.0 15 88.0 16 137.0 17 171.0 18 215.0 19 183.0 20 167.0 21 178.0 22 148.0 23 182.0 24 194.0 25 246.0 26 326.0 27 388.0 28 501.0 29 562.0 30 808.0 31 1034.0 32 1292.0 33 1897.0 34 2730.0 35 4480.0 36 8073.0 37 10024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.332112908940594 22.475493090823196 11.010393291602693 27.18200070863352 2 19.566174528163433 20.887023393701018 35.83609733188529 23.710704746250258 3 17.291379259737592 25.58924535501482 30.499867915113448 26.619507470134142 4 12.771140919897855 20.206052423023863 38.48660072206405 28.536205935014237 5 12.140068684141008 36.01221051395697 36.775367636267575 15.072353165634448 6 27.227097948281433 38.830021427103816 18.09562945786492 15.847251166749832 7 26.463940825970823 29.3756787695559 23.76940914027415 20.390971264199127 8 23.443599753441546 35.433972232821624 19.806862543661392 21.315565470075434 9 23.46414629134991 16.528222137426987 22.17264962282427 37.83498194839884 10-11 24.47386186856086 26.1484047080924 27.761307933898856 21.616425489447884 12-13 23.982212568610763 23.82371070474625 26.933575978161965 25.260500748481025 14-15 22.006809709706772 25.442484369955093 24.465056209457277 28.085649710880862 16-17 21.983327952097216 27.886054771199625 26.820570019665972 23.31004725703719 18-19 21.51516040975667 27.53236079720567 27.316622149167863 23.635856643869793 20-21 21.162934045613312 27.68645983151839 27.454577475124015 23.69602864774428 22-23 22.01414775895976 27.254982535442778 27.535296016906862 23.195573688690597 24-25 21.73823710704746 28.16196542311192 26.964395785024507 23.13540168481611 26-27 20.763744166250845 28.23241069594059 28.35275470368957 22.651090434118995 28-29 22.0449675658223 27.319557368869056 28.694707798878742 21.94076726642989 30-31 22.168246793272477 27.42816049781326 28.16049781326132 22.24309489565294 32-33 20.600839472834544 28.640406234406647 27.928615456866947 22.830138835891866 34-35 22.288590801021456 27.335701077225632 28.21626698758402 22.15944113416889 36-37 22.519005547565236 27.397340690950717 27.246176876339195 22.837476885144852 38-39 21.90994745956735 28.05189468431712 28.44081129472541 21.597346561390122 40-41 21.91435028911914 26.622442689835335 28.754879802753237 22.70832721829229 42-43 21.631101587953857 26.945316856966745 28.37917168100032 23.044409874079076 44-45 20.774017435205025 27.12436525873962 28.47309871143855 23.628518594616807 46-47 21.096891602336434 26.528515659397105 29.347794182394555 23.026798555871906 48-49 20.844462708033696 27.133170917843202 29.252399542105728 22.769966832017378 50-51 20.70063694267516 27.075934133669904 28.644809063958437 23.5786198596965 52-53 19.97857289618128 26.73985147788312 29.400628137016056 23.880947488919546 54-55 19.80979776336259 27.254982535442778 29.221579735243182 23.71363996595145 56-57 19.903724793800816 26.93797880771376 29.057207431976284 24.101088966509142 58-59 19.872904986938273 27.11115677008424 28.58170184038275 24.434236402594735 60-61 19.733482051131528 26.51090434118994 29.145264023012125 24.610349584666412 62-63 19.75402858903989 26.7501247468373 28.93392820452611 24.5619184595967 64-65 20.423258680912266 26.954122516070328 28.13995127535296 24.482667527664447 66-67 20.364554286888374 26.69288796266401 27.77011359300244 25.172444157445184 68-69 19.861164108133494 26.877806803839267 27.366520884088175 25.894508203939065 70-71 21.39481640200769 26.808829140861196 26.800023481757613 24.996330975373507 72-73 21.245120197246763 26.62684551938713 26.11611729137926 26.01191699198685 74-75 21.45058557633039 26.487422583580383 26.33919398867005 25.722797851419177 76 21.69127359182835 26.061815726907163 26.499163462385162 25.747747218879336 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 1.0 16 3.5 17 5.0 18 5.5 19 7.0 20 9.0 21 10.0 22 10.5 23 17.5 24 25.0 25 26.0 26 54.0 27 112.0 28 175.5 29 209.0 30 224.0 31 297.0 32 420.0 33 485.0 34 562.5 35 782.0 36 1021.5 37 1119.0 38 1254.5 39 1556.5 40 1933.5 41 2224.5 42 2305.0 43 2384.5 44 2558.5 45 2702.0 46 2751.0 47 2689.0 48 2555.0 49 2325.0 50 2167.0 51 1968.5 52 1592.5 53 1271.0 54 1127.0 55 1063.5 56 808.0 57 538.5 58 461.0 59 371.0 60 215.5 61 130.0 62 110.0 63 83.5 64 44.0 65 23.0 66 12.0 67 9.0 68 6.5 69 6.0 70 5.5 71 3.0 72 2.0 73 0.5 74 1.5 75 3.0 76 1.5 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 1.0 83 2.0 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5899791599401215 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 34069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.28536205935015 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37794533459001 46.32657254395491 2 12.60603204524034 15.703425401391295 3 5.475966069745524 10.232175878364496 4 2.7851083883129126 6.938859373624116 5 1.6635249764373232 5.18066277260853 6 1.1215834118755892 4.191493733305938 7 0.6361922714420358 2.7737826176289295 8 0.43355325164938735 2.1603217000792507 9 0.2780395852968897 1.558601661334351 >10 0.6220546654099905 4.93410431770818 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0029352197011946342 0.0 10 0.0 0.0 0.0 0.0029352197011946342 0.0 11 0.0 0.0 0.0 0.0029352197011946342 0.0 12 0.0 0.0 0.0 0.0029352197011946342 0.0 13 0.0 0.0 0.0 0.0029352197011946342 0.0 14 0.0 0.0 0.0 0.0029352197011946342 0.0 15 0.0 0.0 0.0 0.0029352197011946342 0.0 16 0.0 0.0 0.0 0.0058704394023892685 0.0 17 0.0 0.0 0.0 0.0058704394023892685 0.0 18 0.0 0.0 0.0 0.011740878804778537 0.0 19 0.0 0.0 0.0 0.011740878804778537 0.0 20 0.0 0.0 0.0 0.011740878804778537 0.0 21 0.0 0.0 0.0 0.011740878804778537 0.0 22 0.0 0.0 0.0 0.014676098505973172 0.0 23 0.0 0.0 0.0 0.02641697731075171 0.0 24 0.0 0.0 0.0 0.029352197011946343 0.0 25 0.0 0.0 0.0 0.029352197011946343 0.0 26 0.0 0.0 0.0 0.035222636414335616 0.0 27 0.0 0.0 0.0 0.04989873492030879 0.0 28 0.0 0.0 0.0 0.06457483342628195 0.0 29 0.0 0.0 0.0 0.0939270304382283 0.0 30 0.0 0.0 0.0 0.12034400774898 0.0 31 0.0 0.0 0.0 0.14382576535853708 0.0 32 0.0 0.0 0.0 0.1790484017728727 0.0 33 0.0 0.0 0.0 0.2377527957967654 0.0 34 0.0 0.0 0.0 0.305262848924242 0.0 35 0.0 0.0 0.0 0.3727729020517186 0.0 36 0.0 0.0 0.0 0.4872464703983093 0.0 37 0.0 0.0 0.0 0.730869705597464 0.0 38 0.0 0.0 0.0 1.0654847515336523 0.0 39 0.0 0.0 0.0 1.4998972673104582 0.0 40 0.0 0.0 0.0 1.9607267603980159 0.0 41 0.0 0.0 0.0 2.4949367460154392 0.0 42 0.0 0.0 0.0 3.0878511256567553 0.0 43 0.0 0.0 0.0 3.7629516569315213 0.0 44 0.0 0.0 0.0 4.440987407907482 0.0 45 0.0 0.0 0.0 5.107282280078664 0.0 46 0.0 0.0 0.0 5.770641932548651 0.0 47 0.0 0.0 0.0 6.434001585018638 0.0 48 0.0 0.0 0.0 7.029851184361149 0.0 49 0.0 0.0 0.0 7.7607208899586135 0.0 50 0.0 0.0 0.0 8.359505709002319 0.0 51 0.0 0.0 0.0 9.01993014177111 0.0 52 0.0 0.0 0.0 9.686225013942293 0.0 53 0.0 0.0 0.0 10.411224280137368 0.0 54 0.0 0.0 0.0 11.074583932607355 0.0 55 0.0 0.0 0.0 11.64108133493792 0.0 56 0.0 0.0 0.0 12.304440987407908 0.0 57 0.0 0.0 0.0 12.917901904957587 0.0 58 0.0 0.0 0.0 13.45211189057501 0.0 59 0.0 0.0 0.0 14.039155830813936 0.0 60 0.0 0.0 0.0 14.696645043881535 0.0 61 0.0 0.0 0.0 15.2073732718894 0.0 62 0.0 0.0 0.0 15.814963750036691 0.0 63 0.0 0.0 0.0 16.322756758343363 0.0 64 0.0 0.0 0.0 16.92447679708826 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGTTG 15 0.002207093 69.994125 47 CGCATCT 15 0.002207093 69.994125 15 GTGGAGG 15 0.002207093 69.994125 28 AACAGGT 15 0.002207093 69.994125 45 TGCAAGG 15 0.002207093 69.994125 33 GAGGCCG 15 0.002207093 69.994125 31 TGGAGGC 20 0.0068968195 52.495594 29 ATCTACT 20 0.0068968195 52.495594 18 ACAGGTT 20 0.0068968195 52.495594 46 CTCGCAT 20 0.0068968195 52.495594 13 GCTACTA 20 0.0068968195 52.495594 47 TAGTGGA 20 0.0068968195 52.495594 26 GCATCTA 20 0.0068968195 52.495594 16 CTACTAT 30 5.8013544E-4 46.66275 20 TCTACTA 45 0.004252312 31.1085 19 CGCTGCC 280 2.2214974E-4 11.249055 70 ACGCTGC 310 5.695335E-4 10.160438 69 TACACAT 625 0.006507404 6.159483 69 >>END_MODULE