FastQCFastQC Report
Wed 25 May 2016
SRR1780534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34069
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT1830.5371452053186181No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT900.2641697731075171RNA PCR Primer, Index 35 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG350.10273268954181221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTCC200.006894530752.542
TCCAGCC200.006894530752.556
ACCTGTT200.006894530752.558
CCTTATT200.006894530752.511
GACCCTA200.006894530752.551
GCTCCGG200.006894530752.568
TGCCGTC703.619789E-1045.045
GCCGTCT703.619789E-1045.046
TCTTCTG703.619789E-1045.050
GGGTTTC757.1122486E-1042.030
CCGTCTT757.1122486E-1042.047
ATGCCGT757.1122486E-1042.044
CGTCTTC757.1122486E-1042.048
CATCTCG701.8357241E-840.036
TATGCCG801.3405952E-939.37543
CTTGAAA801.3405952E-939.37557
CGTATGC753.3725883E-837.33333241
TCGTATG753.3725883E-837.33333240
TTCTGCT852.4265319E-937.05882352
GTCTTCT852.4265319E-937.05882349
Produced by FastQC (version 0.11.5)