##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780534_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34069 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38398544131028 34.0 34.0 34.0 27.0 34.0 2 33.10208694120755 34.0 34.0 34.0 32.0 34.0 3 33.3299773988083 34.0 34.0 34.0 33.0 34.0 4 33.2823094308609 34.0 34.0 34.0 33.0 34.0 5 33.314479438785995 34.0 34.0 34.0 33.0 34.0 6 36.9542986292524 38.0 37.0 38.0 35.0 38.0 7 36.650474037981745 38.0 37.0 38.0 34.0 38.0 8 36.52097214476503 38.0 37.0 38.0 34.0 38.0 9 36.4280137368282 38.0 37.0 38.0 34.0 38.0 10-11 36.053919985910944 38.0 36.5 38.0 32.5 38.0 12-13 35.93204966391734 38.0 36.0 38.0 32.0 38.0 14-15 36.21876192433004 38.0 37.0 38.0 33.0 38.0 16-17 36.387581085444246 38.0 37.0 38.0 34.0 38.0 18-19 36.340382752649035 38.0 37.0 38.0 34.0 38.0 20-21 36.09755202676921 38.0 37.0 38.0 33.0 38.0 22-23 36.0935894801726 38.0 36.5 38.0 33.0 38.0 24-25 35.3228448149344 37.5 35.5 38.0 29.0 38.0 26-27 35.59511579441721 38.0 36.0 38.0 31.0 38.0 28-29 35.431756141947226 38.0 36.0 38.0 31.0 38.0 30-31 35.07021045525258 37.0 35.5 38.0 29.0 38.0 32-33 35.64108133493792 38.0 36.0 38.0 31.0 38.0 34-35 35.732205230561505 38.0 36.5 38.0 32.5 38.0 36-37 35.90714432475271 38.0 37.0 38.0 32.0 38.0 38-39 35.57351257741642 38.0 36.5 38.0 30.0 38.0 40-41 34.937964131615246 37.0 35.5 38.0 27.0 38.0 42-43 35.32054066746896 37.0 35.5 38.0 29.0 38.0 44-45 35.59899028442278 38.0 36.0 38.0 31.0 38.0 46-47 35.900231882356394 38.0 37.0 38.0 32.0 38.0 48-49 35.61840676274619 38.0 36.0 38.0 31.0 38.0 50-51 35.84089641609675 38.0 36.5 38.0 32.0 38.0 52-53 35.77219759899028 38.0 36.5 38.0 31.5 38.0 54-55 35.91391000616396 38.0 36.5 38.0 32.5 38.0 56-57 35.99873785552849 38.0 37.0 38.0 33.0 38.0 58-59 36.115222049370395 38.0 37.0 38.0 34.0 38.0 60-61 35.67995831988024 38.0 36.5 38.0 32.5 38.0 62-63 35.9791012357275 38.0 36.5 38.0 33.0 38.0 64-65 36.02280665707828 38.0 37.0 38.0 33.5 38.0 66-67 35.31028207461328 38.0 36.0 38.0 29.0 38.0 68-69 34.74833426281957 37.0 34.5 38.0 27.0 38.0 70-71 34.785978455487395 37.0 35.0 38.0 27.0 38.0 72-73 34.44536088526226 37.0 34.0 38.0 25.5 38.0 74-75 35.254659661275646 37.5 35.5 38.0 30.0 38.0 76 34.67213595937656 37.0 34.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8074457043509469 1101 2 0.5562800475730185 1101 3 0.022173724260618144 1101 4 0.6326464190871945 1101 5 0.22667891808630714 1101 6 -0.8159869317347841 1101 7 0.2641030509930786 1101 8 1.1056535094102813 1101 9 0.9145464471736346 1101 10-11 0.40439543393954125 1101 12-13 1.0672373533926205 1101 14-15 1.2916837307668274 1101 16-17 0.5308325156826399 1101 18-19 -0.6171838799420399 1101 20-21 0.2105908532265417 1101 22-23 1.099370748173932 1101 24-25 -1.4067152320996072 1101 26-27 0.22688122648872167 1101 28-29 1.262125351021652 1101 30-31 1.7228666620070214 1101 32-33 0.9738755126241969 1101 34-35 1.3048621880141056 1101 36-37 0.9254157569035968 1101 38-39 1.1047267615811407 1101 40-41 2.251721810178573 1101 42-43 1.7789376963390922 1101 44-45 1.2688904526415001 1101 46-47 1.0118080339211915 1101 48-49 -1.0192898817840401 1101 50-51 -1.2247485901060813 1101 52-53 -1.254236483186105 1101 54-55 -0.8303137652452861 1101 56-57 -0.8438204845864377 1101 58-59 -0.6020880771681263 1101 60-61 -0.9223660097472219 1101 62-63 -0.5911796043366095 1101 64-65 -1.3982681077359445 1101 66-67 -0.8458471042490245 1101 68-69 -0.12147073952896648 1101 70-71 -0.8905791315694387 1101 72-73 -1.838934560567651 1101 74-75 -1.1197300395753018 1101 76 -0.7960499942354673 1102 1 -0.5057915171431553 1102 2 0.3779488985996693 1102 3 0.25869622316844243 1102 4 0.7949006785891584 1102 5 0.14183837636502972 1102 6 0.2838820066662606 1102 7 0.04872515671610245 1102 8 0.3312542084054755 1102 9 0.8101340400089327 1102 10-11 1.093080449230058 1102 12-13 0.7451753175691138 1102 14-15 0.1801066228594408 1102 16-17 0.4755245209251058 1102 18-19 0.008111008655596663 1102 20-21 0.9588215216407008 1102 22-23 0.7387766022586888 1102 24-25 -4.406409421702055 1102 26-27 -0.45144992248462756 1102 28-29 0.5579750670548549 1102 30-31 2.2640200040778 1102 32-33 0.6937095012655234 1102 34-35 0.8919744641172116 1102 36-37 -1.8097087516154104 1102 38-39 0.49957167202239106 1102 40-41 1.7192185336386032 1102 42-43 1.3650014796505872 1102 44-45 1.0342901905183055 1102 46-47 1.3584222759482785 1102 48-49 -3.6761706157289993 1102 50-51 -0.2284620020763768 1102 52-53 -0.2876571909187504 1102 54-55 1.0468378293374982 1102 56-57 1.440539497938687 1102 58-59 0.23216268735786372 1102 60-61 -4.938705022416514 1102 62-63 -1.784626524389033 1102 64-65 0.10802284901372872 1102 66-67 1.3883599730773213 1102 68-69 -0.029989743460809848 1102 70-71 -1.0939867331421809 1102 72-73 -5.503023682454938 1102 74-75 -1.6483451553463837 1102 76 0.48573244351027256 1103 1 0.8496002710956816 1103 2 0.11888738559019174 1103 3 -0.06127670509145133 1103 4 0.6237471216633068 1103 5 0.23034792667334614 1103 6 0.6308514757593571 1103 7 0.9073505139126681 1103 8 0.9991741963735876 1103 9 0.3848821224273422 1103 10-11 0.48663587109801654 1103 12-13 -0.40567443427326566 1103 14-15 0.09956819602834344 1103 16-17 0.38024703558897244 1103 18-19 0.9651346212289198 1103 20-21 0.3273355839057004 1103 22-23 1.043672075262144 1103 24-25 -1.8963327184384084 1103 26-27 -0.0752655338547541 1103 28-29 -0.3235108706801526 1103 30-31 1.1926558891733023 1103 32-33 1.2922205555594033 1103 34-35 0.21555695772527628 1103 36-37 -0.9407434936818859 1103 38-39 -0.041487133813078 1103 40-41 0.042237032661006424 1103 42-43 -0.24057830678346903 1103 44-45 1.3555415065056735 1103 46-47 0.9898330144051855 1103 48-49 -1.2020767670299506 1103 50-51 -0.012414476132626362 1103 52-53 0.5625082474930423 1103 54-55 0.678624564184851 1103 56-57 0.9193333015181722 1103 58-59 0.2910813217389787 1103 60-61 -4.490554924657452 1103 62-63 -0.8134278478963282 1103 64-65 0.318242430905741 1103 66-67 0.3990006709266254 1103 68-69 0.9533536164429322 1103 70-71 0.9272194632783339 1103 72-73 -4.429215591012614 1103 74-75 -2.126950960730653 1103 76 -1.8592818443525587 1104 1 -0.618764449131934 1104 2 0.5500620231731759 1104 3 0.06451925751524357 1104 4 -0.4273299681619207 1104 5 0.06973283386749074 1104 6 0.3703176727516322 1104 7 0.5541463410363647 1104 8 0.612776689260734 1104 9 1.2077381040016562 1104 10-11 -0.7793511225342868 1104 12-13 0.8158402656318984 1104 14-15 1.1664967964889854 1104 16-17 0.7736896585397872 1104 18-19 1.016110098806486 1104 20-21 0.1463641708180461 1104 22-23 0.6140893628925497 1104 24-25 0.6563701673494293 1104 26-27 1.3942169210971471 1104 28-29 1.956261517885089 1104 30-31 1.4744605226917926 1104 32-33 1.4147649262408848 1104 34-35 0.8956138605839641 1104 36-37 1.408768767529331 1104 38-39 1.9985480917661107 1104 40-41 2.2957595905799906 1104 42-43 0.6923576097590072 1104 44-45 1.1756200866438675 1104 46-47 1.1657238151097005 1104 48-49 0.6096357380506632 1104 50-51 1.0056725039513665 1104 52-53 0.8939335286202663 1104 54-55 0.3799184268050837 1104 56-57 0.7675923450082109 1104 58-59 -0.26131672730586786 1104 60-61 0.7253711016262514 1104 62-63 0.4844992622513473 1104 64-65 0.797953852122383 1104 66-67 -0.2870513545441824 1104 68-69 2.1751054115926465 1104 70-71 1.9632579404494521 1104 72-73 2.0491410002351884 1104 74-75 0.7564997911999782 1104 76 0.21929829384009736 1105 1 0.9819084097871027 1105 2 0.5219774769872672 1105 3 0.07139412038367254 1105 4 -1.0808807616966263 1105 5 -0.2644293836414633 1105 6 0.4120788078353783 1105 7 0.8131970248658149 1105 8 0.19575043313552243 1105 9 1.155591537776246 1105 10-11 0.7949559059159412 1105 12-13 -0.0762097599659981 1105 14-15 0.6206765668393075 1105 16-17 0.6055480656194376 1105 18-19 0.9209956396534054 1105 20-21 0.6363809922910164 1105 22-23 0.6559236348153163 1105 24-25 0.6381225260124879 1105 26-27 0.23240166475252266 1105 28-29 1.7134106438998629 1105 30-31 1.3288085077718748 1105 32-33 0.9776260393835727 1105 34-35 -0.45628404038875914 1105 36-37 0.7240251121501231 1105 38-39 -0.28397951739061966 1105 40-41 0.3704323032253498 1105 42-43 0.7856802163559422 1105 44-45 1.2233784425277179 1105 46-47 1.3840372796017633 1105 48-49 0.41699330405666757 1105 50-51 1.17074234963264 1105 52-53 1.1281908240199527 1105 54-55 1.3972884260737217 1105 56-57 1.1162722250638026 1105 58-59 1.0073514508554737 1105 60-61 1.360272001209374 1105 62-63 0.07102014281888813 1105 64-65 1.0209480743120949 1105 66-67 1.0219152219203806 1105 68-69 1.1213864033281808 1105 70-71 2.208156645927403 1105 72-73 1.5967165140214803 1105 74-75 0.5153310645123526 1105 76 1.517477186548355 1106 1 -1.12875401825654 1106 2 0.490445558437564 1106 3 0.3970234699841484 1106 4 0.15358955362255955 1106 5 0.19875473870581573 1106 6 0.5340824163928133 1106 7 0.07223989796487729 1106 8 0.9474473143108781 1106 9 -0.0059343665110631605 1106 10-11 0.9720329743926186 1106 12-13 1.8347361975904946 1106 14-15 0.3701395791255919 1106 16-17 0.8756314061126034 1106 18-19 0.8213736790038624 1106 20-21 0.7540027811044965 1106 22-23 1.0765783302358827 1106 24-25 0.605258877932755 1106 26-27 1.2095904266736497 1106 28-29 1.0230592391400037 1106 30-31 1.3564311557656978 1106 32-33 0.38182743125572216 1106 34-35 1.341293071046934 1106 36-37 1.3071078512170473 1106 38-39 0.6093962021729169 1106 40-41 1.395133738056522 1106 42-43 1.2576709372082604 1106 44-45 1.7959362224057216 1106 46-47 1.0711237990621996 1106 48-49 1.556438273555763 1106 50-51 0.590323069625768 1106 52-53 0.9260686485753311 1106 54-55 0.38031961432013617 1106 56-57 0.5809599130095009 1106 58-59 1.272021955194333 1106 60-61 1.7173473513253583 1106 62-63 1.2053529892833623 1106 64-65 0.2488886190324351 1106 66-67 0.8967326460977176 1106 68-69 1.8204155295417692 1106 70-71 1.2510297449908876 1106 72-73 0.8741028874212589 1106 74-75 0.1259934925559989 1106 76 0.48689036637480143 1107 1 1.0831349274085937 1107 2 0.15728255383868373 1107 3 0.1384644510776596 1107 4 0.5146013063052379 1107 5 0.15600222635698202 1107 6 0.0063188326762428915 1107 7 1.2327747301910748 1107 8 0.4813427324679225 1107 9 -0.18069164806446025 1107 10-11 0.9605357848167344 1107 12-13 -0.1300057543768034 1107 14-15 0.04506718941344445 1107 16-17 0.5675493076625955 1107 18-19 0.9813123606594587 1107 20-21 1.2380344622491037 1107 22-23 -0.07769692099899572 1107 24-25 0.9722321363214448 1107 26-27 0.7577082941579008 1107 28-29 1.5740301129264083 1107 30-31 0.8135934790496293 1107 32-33 1.7889131066091508 1107 34-35 0.9111278521745092 1107 36-37 1.355992198006355 1107 38-39 1.0484610974207484 1107 40-41 0.8396917349906019 1107 42-43 1.5627722828052555 1107 44-45 1.1260333097843613 1107 46-47 1.4395022695101645 1107 48-49 1.5324645041808722 1107 50-51 1.1630960214227386 1107 52-53 1.3240843188189046 1107 54-55 1.3045233274865424 1107 56-57 1.4291118462406303 1107 58-59 1.1926487649371396 1107 60-61 1.37362396519012 1107 62-63 0.918093578620784 1107 64-65 1.3959674812364895 1107 66-67 0.6218220686833078 1107 68-69 1.7003337717492357 1107 70-71 1.711300567678677 1107 72-73 2.115075464495014 1107 74-75 -0.38414106714422047 1107 76 1.9044512588973674 1108 1 -0.5866019146966721 1108 2 0.5467562380492126 1108 3 0.10074515283204732 1108 4 0.6874083238490982 1108 5 0.3064408228482094 1108 6 -0.0028916936395475545 1108 7 0.3200554319454554 1108 8 -1.1300607763040063 1108 9 0.06930835193553975 1108 10-11 0.7418954339395469 1108 12-13 1.1202135438688074 1108 14-15 1.022040873623972 1108 16-17 -0.12214367479354848 1108 18-19 0.4054351676770054 1108 20-21 1.362376567512264 1108 22-23 0.027346938650119057 1108 24-25 0.6307847679003942 1108 26-27 -0.74097591636842 1108 28-29 -1.0468032204069289 1108 30-31 0.19012856676892653 1108 32-33 0.8851850364337182 1108 34-35 0.7661717118236382 1108 36-37 1.480772899760737 1108 38-39 1.4559172377716223 1108 40-41 1.549340857797624 1108 42-43 -0.21332420842281152 1108 44-45 0.9676999764510299 1108 46-47 0.8028794624926192 1108 48-49 1.2640434515492913 1108 50-51 1.0115609337034428 1108 52-53 0.347549231099606 1108 54-55 0.25540052046900286 1108 56-57 0.6466557058897564 1108 58-59 0.9187452561652094 1108 60-61 1.6448958950146846 1108 62-63 1.1623918242348168 1108 64-65 0.8398271303592892 1108 66-67 1.0112957528938367 1108 68-69 1.8838864033281837 1108 70-71 -0.04176960775991745 1108 72-73 0.21047020133710959 1108 74-75 -1.4911586110038755 1108 76 0.7325214343359647 1109 1 -1.1055413086360666 1109 2 0.5103926016855738 1109 3 0.5265027285896267 1109 4 0.5222568086975841 1109 5 -0.21704402563663905 1109 6 -0.5081947239425801 1109 7 -0.6822172953272698 1109 8 0.353272557029328 1109 9 0.3374901701173556 1109 10-11 0.7074257369698458 1109 12-13 0.7183196044748641 1109 14-15 0.8512075402906376 1109 16-17 0.8009623858125181 1109 18-19 0.9471018343436697 1109 20-21 1.2521492947849922 1109 22-23 0.6959075447107281 1109 24-25 -0.09156371694809451 1109 26-27 -0.06900621939872309 1109 28-29 -1.7623335234372277 1109 30-31 0.32611341525377924 1109 32-33 0.3825335212822054 1109 34-35 0.033217166369091444 1109 36-37 0.9500910815789183 1109 38-39 1.2278869347413135 1109 40-41 0.5478257062824667 1109 42-43 1.6787212461226417 1109 44-45 1.0623969461479987 1109 46-47 0.8206824927956475 1109 48-49 0.8352555727614117 1109 50-51 -0.25093906629656004 1109 52-53 -0.3626780416276745 1109 54-55 -0.7688419037734278 1109 56-57 -0.1472836880496331 1109 58-59 -0.07784565292569567 1109 60-61 1.0687595313783191 1109 62-63 0.7192100060529967 1109 64-65 1.3822513727835357 1109 66-67 1.0931139347120222 1109 68-69 -0.5149772330354594 1109 70-71 0.7483819073915967 1109 72-73 0.353273231640145 1109 74-75 -0.27903739888265733 1109 76 0.059794161608692775 1110 1 -1.5376745219573955 1110 2 -0.30076851442603925 1110 3 0.29166924524128746 1110 4 -0.12661807879116793 1110 5 0.3776454433102572 1110 6 -0.43861776624680715 1110 7 0.565847511153379 1110 8 1.0998072104946743 1110 9 1.2351499360939542 1110 10-11 -0.9477497805819155 1110 12-13 0.3752052930437273 1110 14-15 1.3625936789044957 1110 16-17 0.6746797575496899 1110 18-19 0.12383450761100079 1110 20-21 1.1803221120667118 1110 22-23 0.13704165812206526 1110 24-25 -1.1100155621326095 1110 26-27 1.2312188031035305 1110 28-29 0.3834360535204695 1110 30-31 -0.648279023990149 1110 32-33 0.8653418021102866 1110 34-35 1.2950083454869983 1110 36-37 1.649461018572616 1110 38-39 1.0053396800158438 1110 40-41 -0.8419132676149204 1110 42-43 0.6514035143494681 1110 44-45 1.2349442008734712 1110 46-47 0.7466500895553239 1110 48-49 1.5510071479189236 1110 50-51 -0.6514341158015142 1110 52-53 0.9253957657530663 1110 54-55 -0.42545756533957757 1110 56-57 0.5671177520943829 1110 58-59 -0.4331111794783524 1110 60-61 -0.09285162973779393 1110 62-63 -0.9566675817057799 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-2.728129743834792 2116 60-61 -3.2894791049853183 2116 62-63 0.2545793242348182 2116 64-65 -1.0784020363073736 2116 66-67 -0.9949542471061648 2116 68-69 -2.495801096671819 2116 70-71 -0.027186274426583168 2116 72-73 -1.997863131996219 2116 74-75 -0.7276169443372069 2116 76 -2.1487285656640367 2117 1 0.7944586913639355 2117 2 0.6558471471401219 2117 3 -0.17349727141037619 2117 4 -0.06865228221150943 2117 5 -0.15340766200027645 2117 6 -0.08092199666985067 2117 7 0.354146341036369 2117 8 0.26236346612023453 2117 9 -2.198873466246276 2117 10-11 -1.4562106266665182 2117 12-13 -1.572589486434218 2117 14-15 -0.12606518698208902 2117 16-17 -0.9172194323693006 2117 18-19 -1.4847163474745102 2117 20-21 0.22487656751226126 2117 22-23 -0.6677288189256316 2117 24-25 -1.4097455351299146 2117 26-27 -2.991733492125995 2117 28-29 -1.0850607961644982 2117 30-31 -0.9784320392916825 2117 32-33 -3.217466478717803 2117 34-35 0.47412625727817925 2117 36-37 -1.7589998275119925 2117 38-39 -0.07665851980413407 2117 40-41 -1.3976288391720786 2117 42-43 -0.3621878447864475 2117 44-45 -0.5921485083974574 2117 46-47 -1.1202265981134403 2117 48-49 -1.2556535181476818 2117 50-51 -0.38275724811474987 2117 52-53 0.26914014019051535 2117 54-55 -0.6506600855916034 2117 56-57 -0.4563745971405453 2117 58-59 -2.023300198380248 2117 60-61 -2.5812404686216865 2117 62-63 -0.5171536303106379 2117 64-65 -1.9813849908528312 2117 66-67 -2.061431519833434 2117 68-69 -0.014977233035452286 2117 70-71 -0.6879817289720336 2117 72-73 0.9260005043674155 2117 74-75 0.37550805566279877 2117 76 0.7961577979723273 2118 1 -1.1078982109929676 2118 2 0.07638586767883737 2118 3 -0.9307363286494308 2118 4 0.10037128681206298 2118 5 0.44255193395932224 2118 6 -0.5482620640099185 2118 7 0.9598702467602749 2118 8 0.30882811258488374 2118 9 -1.3843953517681626 2118 10-11 -1.7270860475419454 2118 12-13 -2.4589531227978583 2118 14-15 0.14935568843878855 2118 16-17 -2.9022362673861366 2118 18-19 -1.471416684174848 2118 20-21 -1.2288271361914411 2118 22-23 0.20836545716864663 2118 24-25 -0.875233750618122 2118 26-27 -4.0030129534054595 2118 28-29 -2.7778217389254465 2118 30-31 -2.4334825443421835 2118 32-33 -1.3134260746773947 2118 34-35 -1.3213282881763675 2118 36-37 -5.833578952091113 2118 38-39 -2.830193873339489 2118 40-41 -3.495103586646824 2118 42-43 -1.43137976397837 2118 44-45 -4.7906333568823065 2118 46-47 -1.5522131300999717 2118 48-49 -1.8637343262284887 2118 50-51 -1.7842723996298915 2118 52-53 -5.178839657789286 2118 54-55 -2.798303183234701 2118 56-57 -1.5163072570732083 2118 58-59 -1.7298658549459063 2118 60-61 -0.8490855864667992 2118 62-63 -2.3533489165059223 2118 64-65 -1.8009136103814498 2118 66-67 -1.2275931359950505 2118 68-69 -4.424909892968117 2118 70-71 -2.7389918299821403 2118 72-73 -0.4399927616258523 2118 74-75 -0.6448623147075736 2118 76 -1.5897007878862581 2119 1 0.5217314186366622 2119 2 -1.6702650385465319 2119 3 0.27060330886041584 2119 4 -0.8980526690586998 2119 5 -0.4628854182865396 2119 6 -0.6743455943487717 2119 7 -2.865582865926882 2119 8 0.17880447192294469 2119 9 -2.1168618608304186 2119 10-11 -0.4140442823725152 2119 12-13 -1.2940595057765805 2119 14-15 -1.8604945873689402 2119 16-17 -0.8007784265665947 2119 18-19 -0.74545135714569 2119 20-21 -3.6793787516366763 2119 22-23 -0.5938410046122939 2119 24-25 0.41810746293585055 2119 26-27 0.30884677866703925 2119 28-29 -0.5376720147331682 2119 30-31 1.4453590632228313 2119 32-33 0.13456446905782826 2119 34-35 -0.3266474371125341 2119 36-37 -0.5189611427924561 2119 38-39 -1.53468559910781 2119 40-41 -1.325481837237838 2119 42-43 -1.5726327190611116 2119 44-45 0.48773543744393777 2119 46-47 -0.5908262112662399 2119 48-49 -0.15333243497553184 2119 50-51 0.873529018809819 2119 52-53 0.7898364651421588 2119 54-55 0.0880246339441797 2119 56-57 -1.4753301097130773 2119 58-59 -0.4964143183028753 2119 60-61 -0.021859424134248684 2119 62-63 -1.639203920446036 2119 64-65 -0.7162012384350334 2119 66-67 0.36874256140447415 2119 68-69 0.9937268288600976 2119 70-71 0.2474147894032086 2119 72-73 1.3917645275782462 2119 74-75 -1.1840664124223181 2119 76 -1.537691331621481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 8.0 24 23.0 25 39.0 26 60.0 27 128.0 28 219.0 29 403.0 30 655.0 31 995.0 32 1574.0 33 2434.0 34 3721.0 35 5547.0 36 8456.0 37 9806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.37506237341865 22.627608676509436 11.467903372567436 27.529425577504472 2 18.456661481111862 20.332266870175232 36.845812909096246 24.36525873961666 3 17.934192374299215 25.568698817106462 30.044908861428276 26.452199947166044 4 12.991282397487453 18.870527458980305 39.52566849628695 28.6125216472453 5 12.304440987407908 37.33599459919575 36.82233115148669 13.537233261909654 6 27.344506736329215 40.07455458041034 18.691479057207435 13.88945962605301 7 26.48448736387919 30.60847104405765 24.215562534855735 18.691479057207435 8 23.02092811646952 36.51413308286125 20.429129120314656 20.035809680354575 9 23.399571457923628 15.961724735096421 22.551292964278378 38.08741084270157 10-11 24.462120989756084 26.392027943291556 27.824415157474537 21.321435909477824 12-13 23.48909565881006 24.035046523232264 27.495670550940737 24.980187267016937 14-15 21.47259972408935 25.410196953241947 24.805541694795856 28.311661627872848 16-17 22.573307112037337 28.38357451055212 26.215914761219878 22.827203616190673 18-19 21.953975755085267 28.245619184595967 27.23883882708621 22.561566233232558 20-21 21.3361120079838 28.147289324605946 26.973201444128094 23.543397223282163 22-23 21.518095629457864 28.192785229974465 27.55584255481523 22.733276585752442 24-25 21.7734597434618 28.636003404854854 27.359182834835188 22.23135401684816 26-27 21.24218497754557 28.437876075024214 27.64389914585107 22.676039801579147 28-29 21.83656696703748 27.768645983151842 28.980891719745223 21.413895330065454 30-31 22.451495494437758 27.13023569814201 27.897795650004404 22.520473157415832 32-33 21.204027121430038 28.357157533241363 28.286712260412695 22.152103084915904 34-35 21.74410754644985 27.202148580821273 29.193695148081833 21.86004872464704 36-37 21.84830784584226 27.45310986527342 28.148756934456543 22.54982535442778 38-39 21.224573659338404 28.17957674131909 28.006398778948604 22.589450820393907 40-41 21.02938154920896 27.403211130353107 28.831195514984298 22.73621180545364 42-43 21.412427720214858 27.230033167982626 28.68590213977516 22.671636972027358 44-45 20.42032346121107 27.469253573629988 29.024920015263145 23.0855029498958 46-47 21.402154451260678 26.594558102673986 29.01758196601016 22.98570548005518 48-49 20.643400158501866 26.264345886289586 29.613431565352666 23.47882238985588 50-51 20.385100824796737 26.546126977604274 29.889342217264964 23.179429980334028 52-53 19.94481786961754 26.578414394317413 30.314949073938184 23.16181866212686 54-55 19.07012239866154 26.955590125920924 30.22982770260354 23.744459772813993 56-57 19.787783615603626 26.844051777275528 29.05867504182688 24.309489565293962 58-59 19.586721066071796 27.156652675452758 29.225982564794972 24.030643693680474 60-61 19.82007103231677 27.27993190290293 28.706448677683525 24.193548387096776 62-63 18.871995068830902 28.354222313540166 28.236813525492384 24.536969092136545 64-65 19.356306319528017 29.060142651677477 26.86459831518389 24.718952713610616 66-67 19.391528955942352 28.690304969326952 26.964395785024507 24.953770289706185 68-69 19.27412016789457 28.470163491737356 26.924770319058382 25.330946021309696 70-71 20.091578854677273 28.59637793888873 25.96495347676774 25.347089729666266 72-73 19.823006252017965 27.4633831342276 26.540256538201884 26.173354075552556 74-75 20.70650738207755 27.387067421996537 26.55346502685726 25.352960169068655 76 20.517185711350493 27.241774046787405 27.341571516628022 24.899468725234083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 2.0 16 2.0 17 0.0 18 1.5 19 6.0 20 13.0 21 17.0 22 16.5 23 29.0 24 35.5 25 29.0 26 51.5 27 109.5 28 168.0 29 191.0 30 237.0 31 320.5 32 461.5 33 565.0 34 629.0 35 820.5 36 1044.0 37 1140.0 38 1345.0 39 1699.0 40 2019.0 41 2377.0 42 2564.0 43 2605.0 44 2666.0 45 2717.5 46 2749.0 47 2682.0 48 2529.0 49 2305.0 50 2167.0 51 1928.5 52 1520.0 53 1203.0 54 1056.0 55 979.0 56 710.0 57 404.0 58 290.0 59 222.0 60 124.0 61 61.5 62 29.0 63 19.0 64 4.5 65 1.0 66 1.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 34069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82291819542693 #Duplication Level Percentage of deduplicated Percentage of total 1 70.08109923033214 39.822125686107604 2 13.988325843277028 15.89714990167014 3 6.544759543364844 11.156770084240804 4 3.3472803347280333 7.608089465496493 5 1.942249083113797 5.518213038245913 6 1.3792034712536805 4.702221961313804 7 0.9401312051242317 3.739469899321964 8 0.5217211632832275 2.3716575185652644 9 0.3925822614804484 2.00769027561713 >10 0.8523167518983419 6.375297190994746 >50 0.0051655560721111625 0.2641697731075171 >100 0.0051655560721111625 0.5371452053186181 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 183 0.5371452053186181 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 90 0.2641697731075171 RNA PCR Primer, Index 35 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 35 0.10273268954181221 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0029352197011946342 0.0 10 0.0 0.0 0.0 0.0029352197011946342 0.0 11 0.0 0.0 0.0 0.0029352197011946342 0.0 12 0.0 0.0 0.0 0.0029352197011946342 0.0 13 0.0 0.0 0.0 0.0029352197011946342 0.0 14 0.0 0.0 0.0 0.0029352197011946342 0.0 15 0.0 0.0 0.0 0.0029352197011946342 0.0 16 0.0 0.0 0.0 0.0058704394023892685 0.0 17 0.0 0.0 0.0 0.0058704394023892685 0.0 18 0.0 0.0 0.0 0.011740878804778537 0.0 19 0.0 0.0 0.0 0.011740878804778537 0.0 20 0.0 0.0 0.0 0.011740878804778537 0.0 21 0.0 0.0 0.0 0.011740878804778537 0.0 22 0.0 0.0 0.0 0.014676098505973172 0.0 23 0.0 0.0 0.0 0.02641697731075171 0.0 24 0.0 0.0 0.0 0.029352197011946343 0.0 25 0.0 0.0 0.0 0.029352197011946343 0.0 26 0.0 0.0 0.0 0.035222636414335616 0.0 27 0.0 0.0 0.0 0.04989873492030879 0.0 28 0.0 0.0 0.0 0.06457483342628195 0.0 29 0.0 0.0 0.0 0.10273268954181221 0.0 30 0.0 0.0 0.0 0.12621444715136929 0.0 31 0.0 0.0 0.0 0.14969620476092635 0.0 32 0.0 0.0 0.0 0.18491884117526197 0.0 33 0.0 0.0 0.0 0.23481757609557075 0.0 34 0.0 0.0 0.0 0.2905867504182688 0.0 35 0.0 0.0 0.0 0.35516158384455077 0.0 36 0.0 0.0 0.0 0.4755055915935308 0.0 37 0.0 0.0 0.0 0.7044527282867122 0.0 38 0.0 0.0 0.0 1.0126507969121488 0.0 39 0.0 0.0 0.0 1.3912941383662567 0.0 40 0.0 0.0 0.0 1.8286418738442571 0.0 41 0.0 0.0 0.0 2.3657870791628755 0.0 42 0.0 0.0 0.0 2.9352197011946344 0.0 43 0.0 0.0 0.0 3.6191258915729843 0.0 44 0.0 0.0 0.0 4.30009686225014 0.0 45 0.0 0.0 0.0 4.93997475711057 0.0 46 0.0 0.0 0.0 5.5886583110745836 0.0 47 0.0 0.0 0.0 6.237341865038598 0.0 48 0.0 0.0 0.0 6.841997123484693 0.0 49 0.0 0.0 0.0 7.6110246851976875 0.0 50 0.0 0.0 0.0 8.224485602747366 0.0 51 0.0 0.0 0.0 8.858493058205406 0.0 52 0.0 0.0 0.0 9.539464028882563 0.0 53 0.0 0.0 0.0 10.273268954181221 0.0 54 0.0 0.0 0.0 10.933693386950013 0.0 55 0.0 0.0 0.0 11.514866887786551 0.0 56 0.0 0.0 0.0 12.163550441750566 0.0 57 0.0 0.0 0.0 12.803428336610995 0.0 58 0.0 0.0 0.0 13.343508761630808 0.0 59 0.0 0.0 0.0 13.93348792157093 0.0 60 0.0 0.0 0.0 14.582171475534944 0.0 61 0.0 0.0 0.0 15.116381461152367 0.0 62 0.0 0.0 0.0 15.723971939299657 0.0 63 0.0 0.0 0.0 16.240570606709912 0.0 64 0.0 0.0 0.0 16.80413278933928 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGTCC 20 0.0068945307 52.5 42 TCCAGCC 20 0.0068945307 52.5 56 ACCTGTT 20 0.0068945307 52.5 58 CCTTATT 20 0.0068945307 52.5 11 GACCCTA 20 0.0068945307 52.5 51 GCTCCGG 20 0.0068945307 52.5 68 TGCCGTC 70 3.619789E-10 45.0 45 GCCGTCT 70 3.619789E-10 45.0 46 TCTTCTG 70 3.619789E-10 45.0 50 GGGTTTC 75 7.1122486E-10 42.0 30 CCGTCTT 75 7.1122486E-10 42.0 47 ATGCCGT 75 7.1122486E-10 42.0 44 CGTCTTC 75 7.1122486E-10 42.0 48 CATCTCG 70 1.8357241E-8 40.0 36 TATGCCG 80 1.3405952E-9 39.375 43 CTTGAAA 80 1.3405952E-9 39.375 57 CGTATGC 75 3.3725883E-8 37.333332 41 TCGTATG 75 3.3725883E-8 37.333332 40 TTCTGCT 85 2.4265319E-9 37.058823 52 GTCTTCT 85 2.4265319E-9 37.058823 49 >>END_MODULE