FastQCFastQC Report
Wed 25 May 2016
SRR1780533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441876
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG401.8119308E-533.0147131
CGTTAAT551.3622957E-732.02152624
CGGTAAG602.9500916E-729.31981328
CAACGCC602.9795046E-729.2866349
TGTAAGA300.005785993829.2866344
GGACCAT703.635614E-828.2406866
ACATTGT407.0626475E-427.47487443
CGAACAA756.892515E-826.43875117
TATCACG508.291614E-526.4177634
TGTAACG756.945811E-826.4177612
TCACGAA609.692516E-625.6810236
ACGAACA801.2860801E-724.78914316
CATGTAA801.3074168E-724.74700410
GACCATG801.3261706E-724.71067
TCCGTAT450.001389507224.46643813
AACATTG450.001404267924.42211242
GAACATT450.001404267924.42211241
TCAGATG450.001409836324.4055294
ATCCAGT551.5671499E-424.0543420
TATCCAG551.5719878E-424.04341519
Produced by FastQC (version 0.11.5)