FastQCFastQC Report
Wed 25 May 2016
SRR1780533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441876
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT42420.9599978274448036No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG10780.2439598439381184No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT7820.17697272537997086RNA PCR Primer, Index 45 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC7030.1590944065756004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1150.036.3483944
GCCGGCA250.002351004935.20054211
TTCGTAG250.002351004935.20054235
TCGTTGT505.9366357E-835.1965634
GCCGTCT1250.033.44051743
ATCGCAT401.8172806E-533.0005139
CATCGTT551.3715544E-731.99687232
TAGTCCA353.2164276E-431.42549929
AATCGTC353.2164276E-431.42549928
GTCACTT300.005740685429.33378824
AGTCTAT300.005740685429.33378840
TCGCATA454.0633415E-529.33378640
CGTTGAT554.955469E-627.99726316
ATCACGC407.02439E-427.50042540
CGTCCCA407.029039E-427.49731331
ATGCCGT5750.026.78302244
TCTATTC508.3288E-526.4004083
TGAGTTA508.3352155E-526.39741919
GCGTTGA609.733543E-625.66706515
ATGGACT450.001396685724.44482241
Produced by FastQC (version 0.11.5)