FastQCFastQC Report
Wed 25 May 2016
SRR1780532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTAA200.00675518252.50000437
ACGAGCA400.00230804235.00000469
CGAGCAG400.00230804235.00000470
GCCGACG1159.170789E-418.2608770
TGCCGAC1401.6455528E-417.569
CTGCCGA1452.1525304E-416.89655168
GCTGCCG1502.789033E-416.33333267
CGCTGCC1553.5817525E-415.80645366
ACGCTGC1604.5616415E-415.31250165
CTGACGC1604.5616415E-415.31250162
GACGCTG1655.7643815E-414.84848664
TGACGCT1655.7643815E-414.84848663
TCTGACG1707.2309095E-414.41176561
CATCTGA1900.001676938812.89473659
ATCTGAC1900.001676938812.89473660
ACATCTG2304.898212E-412.17391458
CACATCT2509.878138E-411.257