##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780532_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10113 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.064075941857016 34.0 27.0 34.0 12.0 34.0 2 30.92069613368931 34.0 32.0 34.0 23.0 34.0 3 31.036388806486702 34.0 32.0 34.0 23.0 34.0 4 31.182537328191437 34.0 32.0 34.0 23.0 34.0 5 31.323346188074755 34.0 33.0 34.0 23.0 34.0 6 33.16701275585879 37.0 33.0 38.0 21.0 38.0 7 32.81845149807179 37.0 32.0 38.0 21.0 38.0 8 31.25353505389103 36.0 27.0 38.0 11.0 38.0 9 32.55473153366953 37.0 31.0 38.0 12.0 38.0 10-11 33.0758429743894 37.0 34.0 38.0 21.0 38.0 12-13 33.66226638979531 37.0 34.0 38.0 22.0 38.0 14-15 33.389004251952926 37.0 34.0 38.0 21.0 38.0 16-17 33.21556412538317 37.0 34.0 38.0 21.0 38.0 18-19 33.161722535350535 37.0 34.0 38.0 21.0 38.0 20-21 33.250469692475036 37.0 34.0 38.0 21.0 38.0 22-23 33.27820626915851 37.0 34.0 38.0 21.0 38.0 24-25 33.77573420350045 37.0 34.0 38.0 21.5 38.0 26-27 33.91906457035499 37.0 34.0 38.0 22.5 38.0 28-29 34.091219222782556 37.0 34.0 38.0 23.0 38.0 30-31 34.018095520617024 37.0 34.0 38.0 23.0 38.0 32-33 33.552111144071986 37.0 34.0 38.0 21.5 38.0 34-35 33.510283793137546 37.0 34.0 38.0 21.5 38.0 36-37 33.39073469791358 37.0 34.0 38.0 21.0 38.0 38-39 33.96326510432117 37.0 34.0 38.0 23.0 38.0 40-41 33.34762187283694 37.0 34.0 38.0 21.0 38.0 42-43 33.80594284584198 37.0 34.0 38.0 22.5 38.0 44-45 32.61856026896075 37.0 31.5 38.0 16.0 38.0 46-47 33.44877879956492 37.0 34.0 38.0 21.5 38.0 48-49 33.173539009196084 37.0 34.0 38.0 21.0 38.0 50-51 33.08355581924256 37.0 33.0 38.0 21.0 38.0 52-53 33.116879264313255 37.0 33.5 38.0 21.0 38.0 54-55 32.84836349253436 37.0 32.5 38.0 21.0 38.0 56-57 32.920251161870866 37.0 32.0 38.0 16.0 38.0 58-59 32.79298922179373 37.0 32.0 38.0 16.0 38.0 60-61 32.70923563729853 37.0 31.5 38.0 16.0 38.0 62-63 32.857856224661326 37.0 32.0 38.0 20.5 38.0 64-65 32.79575793533077 37.0 32.0 38.0 20.0 38.0 66-67 32.82448333827746 37.0 32.0 38.0 19.5 38.0 68-69 32.25215069712252 37.0 30.5 38.0 15.0 38.0 70-71 32.218036191041236 37.0 31.0 38.0 11.0 38.0 72-73 32.23652724216355 37.0 31.0 38.0 15.0 38.0 74-75 31.691436764560464 36.0 29.0 38.0 14.5 38.0 76 30.607831504004746 35.0 26.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.1862547113841124 1101 2 0.5818923703146517 1101 3 0.4687998961119142 1101 4 -0.9682017988131157 1101 5 0.5431939446984551 1101 6 0.7051340236218451 1101 7 0.6063686698312338 1101 8 0.1380896599169681 1101 9 1.1077273520393902 1101 10-11 0.7823528388691727 1101 12-13 -0.5180160639066287 1101 14-15 -0.5584854520907214 1101 16-17 -2.5528707983031502 1101 18-19 0.8954412589300418 1101 20-21 0.47649935742428795 1101 22-23 -0.03285493143218332 1101 24-25 -1.687199532565316 1101 26-27 -0.760162685896077 1101 28-29 1.9173451396383001 1101 30-31 0.2595409010447369 1101 32-33 1.6532168588014073 1101 34-35 -1.2861155938574669 1101 36-37 0.49133772861127767 1101 38-39 -0.21078800123887476 1101 40-41 0.859207551947847 1101 42-43 -0.18671993180316093 1101 44-45 0.23839654351669992 1101 46-47 2.657475151108592 1101 48-49 -0.4072215364590477 1101 50-51 0.08034828899914714 1101 52-53 0.6856632613716016 1101 54-55 0.2655703666180358 1101 56-57 -0.054115442209671016 1101 58-59 1.3218555040332518 1101 60-61 1.0830688612743344 1101 62-63 -1.686888542677572 1101 64-65 0.7447240880760262 1101 66-67 -0.9909821153575606 1101 68-69 1.188566884972012 1101 70-71 0.6635400666829625 1101 72-73 -1.3950046257346749 1101 74-75 -1.8349209599685103 1101 76 -1.954574231767431 1102 1 -0.019588044717444575 1102 2 -2.584774296352016 1102 3 0.43546656277858276 1102 4 1.331798201186885 1102 5 1.14319394469846 1102 6 0.2384673569551765 1102 7 2.6397020031645653 1102 8 1.7047563265836345 1102 9 0.8410606853727245 1102 10-11 -2.0343138277974973 1102 12-13 -6.851349397239961 1102 14-15 -0.59181878542406 1102 16-17 -1.2362041316364838 1102 18-19 -0.30455874106996106 1102 20-21 1.5098326907576194 1102 22-23 -0.3328549314321876 1102 24-25 1.1461338007680197 1102 26-27 -7.693496019229407 1102 28-29 0.2506784729716287 1102 30-31 0.12620756771140407 1102 32-33 -6.896783141198593 1102 34-35 -0.6027822605241298 1102 36-37 -0.3086622713887195 1102 38-39 0.05587866542779096 1102 40-41 0.44254088528118274 1102 42-43 0.9966134015301762 1102 44-45 -8.444936789816637 1102 46-47 0.4908084844419207 1102 48-49 -0.3572215364590505 1102 50-51 -8.386318377667514 1102 52-53 -3.147670071961727 1102 54-55 1.6822370332847072 1102 56-57 1.129217891123659 1102 58-59 1.7885221706999204 1102 60-61 1.3497355279410002 1102 62-63 -4.703555209344238 1102 64-65 0.21139075474269475 1102 66-67 0.2756845513091051 1102 68-69 -8.044766448361322 1102 70-71 -1.2031265999837046 1102 72-73 0.204995374265323 1102 74-75 -8.368254293301845 1102 76 -3.1545742317674303 1103 1 -0.2811265062559052 1103 2 -0.6975948091725286 1103 3 -1.76453343722142 1103 4 -0.7887146193259333 1103 5 -1.2952675937630822 1103 6 -3.243583925096104 1103 7 0.7268814903440557 1103 8 2.120140941968252 1103 9 -0.8179136736016339 1103 10-11 1.146455402971732 1103 12-13 -0.737246833137398 1103 14-15 -1.0046392982445695 1103 16-17 -0.36569131112366193 1103 18-19 -2.1545587410699625 1103 20-21 -2.640167309242379 1103 22-23 -1.1802908288680882 1103 24-25 -1.0436097889755729 1103 26-27 -0.8101626858960742 1103 28-29 0.3750374473306053 1103 30-31 0.7877460292498668 1103 32-33 -6.676270320685774 1103 34-35 -0.9104745682164364 1103 36-37 -1.0471238098502624 1103 38-39 0.4802376397867647 1103 40-41 0.8540793468196455 1103 42-43 0.2594339143506872 1103 44-45 -4.544936789816635 1103 46-47 -0.7835504899170544 1103 48-49 -0.5636317928693089 1103 50-51 0.4854764941273544 1103 52-53 -0.6797213540130116 1103 54-55 0.5155703666180358 1103 56-57 0.5240896859954631 1103 58-59 0.3923683245460765 1103 60-61 1.5138380920435637 1103 62-63 1.3067012009121726 1103 64-65 0.6088266521785854 1103 66-67 0.2859409615655153 1103 68-69 -3.997330550925426 1103 70-71 -0.4390240358811397 1103 72-73 1.1511492204191711 1103 74-75 -2.5733824984300497 1103 76 0.2582462810530828 1104 1 -3.143117456482152 1104 2 0.7367943310989666 1104 3 -0.7743373587900457 1104 4 0.7572883972653202 1104 5 0.13535080744355454 1104 6 -0.6183953881428579 1104 7 1.6514667090469217 1104 8 1.3675014246228514 1104 9 -1.4099197067841374 1104 10-11 -1.020588337601417 1104 12-13 -0.6248788090046631 1104 14-15 -0.4673089815024909 1104 16-17 -1.0293413865384409 1104 18-19 0.7817157687339602 1104 20-21 1.8794405338948792 1104 22-23 0.1975372254305512 1104 24-25 -0.8215132580555107 1104 26-27 1.1359157454764741 1104 28-29 -0.280694076047979 1104 30-31 -1.6247728244454578 1104 32-33 0.008118819585725134 1104 34-35 0.05898244535821817 1104 36-37 -0.8027799184475484 1104 38-39 -1.0872585894741675 1104 40-41 1.970972257830205 1104 42-43 0.5652408525105628 1104 44-45 -5.476309338836245 1104 46-47 0.4996320138536916 1104 48-49 1.7368961105997727 1104 50-51 0.545054171352092 1104 52-53 0.32782012411670536 1104 54-55 0.30968801367686183 1104 56-57 0.8713747538687642 1104 58-59 1.7140123667783556 1104 60-61 0.20463748872531795 1104 62-63 0.15134675144008014 1104 64-65 0.5741358527819074 1104 66-67 0.008037492485570397 1104 68-69 -4.223197820910343 1104 70-71 -0.9099893450817405 1104 72-73 -0.15578893946016592 1104 74-75 0.9356672753256063 1104 76 1.314053219212962 1105 1 0.29152306639366543 1105 2 -0.406996518574239 1105 3 -2.0700889927769737 1105 4 -1.4404240210353372 1105 5 -1.3401393886348778 1105 6 -1.378199309711487 1105 7 0.07303533649790239 1105 8 0.25475632658363523 1105 9 -0.9033837590717191 1105 10-11 -0.13986938335305155 1105 12-13 -2.9819049527955173 1105 14-15 -1.3251521187573871 1105 16-17 -0.5889819094142652 1105 18-19 -1.126780963292184 1105 20-21 0.6792771352020637 1105 22-23 -0.7272993758766262 1105 24-25 -0.9677550881208674 1105 26-27 -0.07405157478496704 1105 28-29 -1.8770993048061513 1105 30-31 -1.9654590989552645 1105 32-33 -2.8162275856430377 1105 34-35 -1.019448927190794 1105 36-37 -0.008662271388722331 1105 38-39 -0.9941213345722062 1105 40-41 0.07309644083673561 1105 42-43 -0.30894215402538094 1105 44-45 0.9272854324055828 1105 46-47 0.21858626221970212 1105 48-49 0.0955562413187252 1105 50-51 -0.8863183776675143 1105 52-53 -1.9254478497395056 1105 54-55 -1.720540744493075 1105 56-57 -0.618004331098561 1105 58-59 -1.2892556070778554 1105 60-61 0.019179972385444444 1105 62-63 -0.4229996537886862 1105 64-65 -1.6497203563684195 1105 66-67 -1.3743154486908935 1105 68-69 1.1052335516386833 1105 70-71 0.8440956222385196 1105 72-73 -0.1533379590680113 1105 74-75 -1.7293654044129525 1105 76 -4.921240898434096 1106 1 -1.4970073995561535 1106 2 -0.10950547914771391 1106 3 0.837617100412988 1106 4 0.7629809968858083 1106 5 1.2539466328705018 1106 6 0.5115856365250693 1106 7 2.9816374870355347 1106 8 1.3864767566911596 1106 9 2.078695093974872 1106 10-11 1.6468689679014261 1106 12-13 1.747037699534232 1106 14-15 0.6802242253286295 1106 16-17 2.6955162984710412 1106 18-19 0.4207100761343412 1106 20-21 1.5910154864565413 1106 22-23 0.9053171115785688 1106 24-25 2.3848434781873777 1106 26-27 2.4532781743189744 1106 28-29 -1.3627623872434214 1106 30-31 -0.07164189465418502 1106 32-33 2.0408512674035606 1106 34-35 2.590766126572646 1106 36-37 1.5235957931274058 1106 38-39 1.7908249019869302 1106 40-41 1.4946914229155936 1106 42-43 2.2734951219602806 1106 44-45 3.288396543516697 1106 46-47 2.4650020328290267 1106 48-49 1.531488140960306 1106 50-51 2.506154740612047 1106 52-53 0.17222240115655296 1106 54-55 1.6929897214567475 1106 56-57 1.8851318696182915 1106 58-59 0.24389851478593982 1106 60-61 3.0357570333173456 1106 62-63 2.837305005709524 1106 64-65 1.9802079590437671 1106 66-67 2.020845841631683 1106 68-69 3.1482443043268518 1106 70-71 1.6312820021668344 1106 72-73 1.4609093527599555 1106 74-75 1.0941112980960028 1106 76 -0.018015091982483966 1107 1 0.41725406054571224 1107 2 0.17487482645500307 1107 3 0.08634375576103892 1107 4 1.0142543415377645 1107 5 -0.5550516693366312 1107 6 0.46829191835868755 1107 7 -0.49714010209859083 1107 8 0.10124755465380986 1107 9 -0.23964106901323845 1107 10-11 0.01656336518495749 1107 12-13 1.2907558659179301 1107 14-15 0.1836198110671674 1107 16-17 1.502392359591589 1107 18-19 0.36298511857916793 1107 20-21 0.9212361995295453 1107 22-23 -0.2126794928356972 1107 24-25 -0.4407083044951463 1107 26-27 0.06703029656006265 1107 28-29 -0.16072503580029718 1107 30-31 -0.09923102877982615 1107 32-33 1.3672519465207031 1107 34-35 0.6340598447390278 1107 36-37 1.2018640444007573 1107 38-39 1.7865804198137525 1107 40-41 -0.4407924480521501 1107 42-43 0.44836778749508 1107 44-45 0.564712332990382 1107 46-47 0.9408084844419236 1107 48-49 -0.3598531154064233 1107 50-51 1.7408746047886225 1107 52-53 2.2426808052312524 1107 54-55 0.9497808929338305 1107 56-57 1.6546564876148864 1107 58-59 1.3130835742086973 1107 60-61 1.0242969314497756 1107 62-63 0.14205882574348294 1107 64-65 1.4657767196549685 1107 66-67 2.336210867098586 1107 68-69 2.1490932007614845 1107 70-71 0.32143480352507225 1107 72-73 0.2041181812828725 1107 74-75 1.4519211452946479 1107 76 0.7103380489343252 1108 1 -0.8195880447174453 1108 2 -0.15699651857423902 1108 3 -1.1256445483325308 1108 4 -0.49597957659089076 1108 5 0.46541616692067933 1108 6 1.1773562458440665 1108 7 0.656368669831231 1108 8 -1.7730214511941433 1108 9 -1.1811615368494977 1108 10-11 -0.7648693833530515 1108 12-13 -0.4263493972399601 1108 14-15 1.0498478812426129 1108 16-17 -0.49175968719203667 1108 18-19 0.5121079255967089 1108 20-21 0.08205491297984224 1108 22-23 -1.185632709209969 1108 24-25 -0.39831064367642455 1108 26-27 -1.1851626858960742 1108 28-29 -1.2520993048061513 1108 30-31 0.4789853454891855 1108 32-33 -0.7467831411985912 1108 34-35 -1.1722267049685726 1108 36-37 -0.13366227138872233 1108 38-39 0.11698977653890097 1108 40-41 0.8508742186145213 1108 42-43 0.9549467348635048 1108 44-45 -0.7532701231499672 1108 46-47 0.968586262219695 1108 48-49 1.1927784635409466 1108 50-51 -0.011318377667514312 1108 52-53 0.06066326137160871 1108 54-55 -0.9149851889375213 1108 56-57 0.45144011334588185 1108 58-59 -1.7337000515223053 1108 60-61 0.11640219460766588 1108 62-63 -1.2146663204553505 1108 64-65 -0.5524981341462052 1108 66-67 -0.6382043375797792 1108 68-69 0.21634466274979047 1108 70-71 1.066317844460741 1108 72-73 -0.12556018129022561 1108 74-75 0.6317457066981618 1108 76 0.43987021267701465 1109 1 -0.5624451875745891 1109 2 -0.6228695344472541 1109 3 0.7878475151595339 1109 4 0.21275058213926812 1109 5 0.7765272780317929 1109 6 2.1051340236218437 1109 7 -0.10315513969257495 1109 8 1.209518231345541 1109 9 3.693441637753679 1109 10-11 1.7252099817263087 1109 12-13 0.9557934599028997 1109 14-15 0.08437169076641737 1109 16-17 0.24712920169684338 1109 18-19 0.7359174494062302 1109 20-21 1.697927928852856 1109 22-23 1.6219069733297147 1109 24-25 1.6461338007680197 1109 26-27 0.885075409342015 1109 28-29 1.0340118063049601 1109 30-31 0.16430280580664203 1109 32-33 1.4698835254680702 1109 34-35 0.05436059661872861 1109 36-37 0.7199091571827054 1109 38-39 1.3106405701896975 1109 40-41 0.9592075519478485 1109 42-43 1.965661020577791 1109 44-45 0.05982511494526932 1109 46-47 -0.9234772298437903 1109 48-49 -0.2215072507447644 1109 50-51 1.4089197175705763 1109 52-53 1.5404251661335095 1109 54-55 0.9869989380466109 1109 56-57 0.1482655101712851 1109 58-59 0.6051888373665903 1109 60-61 1.2164021946076602 1109 62-63 -0.8940313998204275 1109 64-65 0.4875812309331664 1109 66-67 -0.8243154486908963 1109 68-69 1.381424027829155 1109 70-71 0.12068292382581802 1109 72-73 0.16690013617008503 1109 74-75 2.5222218971743544 1109 76 -0.7212408984340968 1110 1 1.3518405267111255 1110 2 1.4342733226956028 1110 3 -0.4121524848404654 1110 4 0.755607724996409 1110 5 0.37652727803179076 1110 6 1.1337054521932757 1110 7 2.096844860307421 1110 8 4.409518231345537 1110 9 -2.9065583622463222 1110 10-11 -1.3462185897022607 1110 12-13 2.170079174188615 1110 14-15 0.627228833623569 1110 16-17 1.5899863445539921 1110 18-19 2.1787745922633732 1110 20-21 -0.24492921400428713 1110 22-23 1.8790498304725745 1110 24-25 2.6747052293394518 1110 26-27 0.15650398077058725 1110 28-29 2.53401180630496 1110 30-31 1.2357313772352185 1110 32-33 2.641312096896648 1110 34-35 2.2257891680472994 1110 36-37 0.8056234428969944 1110 38-39 2.1677834273325516 1110 40-41 1.2734932662335652 1110 42-43 1.6228038777206493 1110 44-45 2.9169679720881305 1110 46-47 2.0765227701562097 1110 48-49 2.764207034969516 1110 50-51 1.366062574713439 1110 52-53 0.5404251661335095 1110 54-55 0.41557036661803437 1110 56-57 1.8339797958855684 1110 58-59 -2.409096876919129 1110 60-61 1.8021164803219492 1110 62-63 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-2.121942578590641 2116 66-67 0.5090178846424394 2116 68-69 -0.4947664483613181 2116 70-71 -3.8364599333170375 2116 72-73 0.5383287075986587 2116 74-75 0.3984123733648204 2116 76 0.4787591015659025 2117 1 0.7518405267111277 2117 2 0.005701894124175766 2117 3 1.8307046580166784 2117 4 0.6270362964249792 2117 5 -2.352044150539637 2117 6 -2.0805802620924396 2117 7 -2.3317265682640063 2117 8 -1.5904817686544597 2117 9 1.922013066325107 2117 10-11 1.0466385531548852 2117 12-13 1.8772220313314705 2117 14-15 1.8772288336235619 2117 16-17 1.0757006302682797 2117 18-19 1.964488877977658 2117 20-21 2.3836422145671463 2117 22-23 1.9790498304725759 2117 24-25 1.581848086482303 2117 26-27 3.0350754093420207 2117 28-29 1.8768689491621018 2117 30-31 2.8500170915209324 2117 32-33 3.6341692397537884 2117 34-35 -0.13849654623841445 2117 36-37 3.0270520143255695 2117 38-39 2.982069141618261 2117 40-41 -3.083649590909296 2117 42-43 -2.6343389794222105 2117 44-45 3.2169679720881277 2117 46-47 1.512237055870493 2117 48-49 1.1570641778266548 2117 50-51 2.923205431856289 2117 52-53 2.1261394518477985 2117 54-55 -1.2344296333819642 2117 56-57 0.9196940815998573 2117 58-59 -0.9519540197762737 2117 60-61 2.2592593374648047 2117 62-63 0.8488257430367128 2117 64-65 0.5447240880760233 2117 66-67 -2.6957440201194665 2117 68-69 0.2957097421148731 2117 70-71 1.5563972095401013 2117 72-73 3.4597572790272295 2117 74-75 1.250793325745775 2117 76 -1.492669469862669 2118 1 0.32326909813969706 2118 2 1.1485590369813181 2118 3 -0.026438199126179285 2118 4 -1.1586779892893055 2118 5 -0.42347272196820995 2118 6 2.7765625950504145 2118 7 0.8111305745931361 2118 8 -2.376196054368748 2118 9 -1.077986933674893 2118 10-11 -0.3462185897022607 2118 12-13 3.0200791741886093 2118 14-15 0.9486574050521384 2118 16-17 1.0757006302682797 2118 18-19 -0.17836826487948798 2118 20-21 -5.616357785432857 2118 22-23 0.050478401901145276 2118 24-25 1.2604195150537336 2118 26-27 2.142218266484882 2118 28-29 -0.5874167651236064 2118 30-31 2.0643028058066406 2118 32-33 3.2413120968966425 2118 34-35 1.3972177394758702 2118 36-37 1.955623442896993 2118 38-39 1.9463548559039836 2118 40-41 -0.4407924480521501 2118 42-43 0.7585181634349354 2118 44-45 -0.568746313626157 2118 46-47 -0.059191515558076446 2118 48-49 -1.592935822173338 2118 50-51 -0.4339374252865653 2118 52-53 -2.3381462624379203 2118 54-55 -0.3058582048105336 2118 56-57 -0.4731630612572886 2118 58-59 1.726617408795157 2118 60-61 -1.4193120911066188 2118 62-63 1.2416828858938587 2118 64-65 0.33043837379031515 2118 66-67 -1.0171725915480394 2118 68-69 1.295709742114866 2118 70-71 1.5206829238258237 2118 72-73 -0.9330998638299093 2118 74-75 2.0365076114600598 2118 76 1.7216162444230498 2119 1 3.1804119552825547 2119 2 2.348559036981321 2119 3 2.002133229445249 2119 4 1.9984648678535493 2119 5 2.4765272780317886 2119 6 -2.0948659763781556 2119 7 -3.3602979968354347 2119 8 3.3380896599169674 2119 9 -1.9922726479606077 2119 10-11 0.8323528388691699 2119 12-13 1.748650602760037 2119 14-15 2.2415145479092757 2119 16-17 -1.0028707983031495 2119 18-19 -1.3212254077366268 2119 20-21 -0.42350064257571773 2119 22-23 0.8504784019011424 2119 24-25 1.7461338007680212 2119 26-27 -0.5934960192294128 2119 28-29 1.8840118063049687 2119 30-31 -1.4571257656219316 2119 32-33 1.8198835254680787 2119 34-35 2.1972177394758674 2119 36-37 -2.2086622713887216 2119 38-39 1.0392119987611252 2119 40-41 -0.0907924480521487 2119 42-43 -3.1200532651364945 2119 44-45 -2.3116034564833043 2119 46-47 -0.45919151555808213 2119 48-49 0.44277846354094663 2119 50-51 0.6803482889991557 2119 52-53 -1.4810034052950627 2119 54-55 0.9155703666180344 2119 56-57 1.3125512244569961 2119 58-59 2.805188837366593 2119 60-61 1.5164021946076645 2119 62-63 0.8631114573224252 2119 64-65 0.9947240880760262 2119 66-67 1.3256845513091093 2119 68-69 1.5385668849720062 2119 70-71 2.4135400666829625 2119 72-73 1.5383287075986658 2119 74-75 0.2650790400314875 2119 76 2.2787591015659068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 19.0 15 38.0 16 85.0 17 95.0 18 122.0 19 103.0 20 102.0 21 100.0 22 109.0 23 90.0 24 122.0 25 143.0 26 157.0 27 183.0 28 260.0 29 272.0 30 345.0 31 438.0 32 617.0 33 793.0 34 1039.0 35 1402.0 36 2065.0 37 1408.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.64312008755348 22.137100785991443 10.645706894836335 30.574072231618743 2 18.57015722337585 23.35607633738752 34.10461781864926 23.96914862058736 3 17.996637990705032 23.761495105309997 30.040541876792247 28.20132502719272 4 13.497478493028774 17.067141303273015 37.031543557796894 32.403836645901315 5 13.111836250370809 36.08227034510036 34.45070701077821 16.355186393750618 6 27.430040541876792 36.24048254721645 16.760605161673094 19.56887174923366 7 26.075348561257787 30.950262038959753 22.485909225749037 20.488480174033423 8 22.495797488381292 32.49283100959162 20.191832295065755 24.819539206961334 9 22.624344902600612 17.205576980124594 22.228814397310394 37.941263719964404 10-11 23.771383367942253 26.174231187580343 27.247107683180065 22.80727776129734 12-13 22.372194205478095 23.979036883219617 27.47453772372194 26.174231187580343 14-15 20.36487689113023 25.996242460199742 25.402946702264412 28.235933946405616 16-17 22.23375852862652 28.349648966676554 26.114901611786813 23.301690892910116 18-19 20.81973697221398 28.102442400870164 27.316325521605854 23.761495105309997 20-21 21.047167012755857 27.336102046870366 27.494314248986452 24.122416691387325 22-23 22.530406407594185 27.3064372589736 27.494314248986452 22.66884208444576 24-25 21.180658558291306 28.483140512212003 27.26194007712845 23.07426085236824 26-27 20.078117274794817 29.56590527044398 27.583308612676756 22.772668842084446 28-29 21.8382280233363 27.148225056857513 28.839117966973205 22.174428952832987 30-31 21.408088598833185 27.158113319489768 28.1617719766637 23.27202610501335 32-33 19.83585484030456 28.82428557302482 27.860179966379906 23.479679620290714 34-35 21.70473647780085 27.840403441115395 28.00850390586374 22.446356175220014 36-37 22.23375852862652 27.489370117670326 27.642638188470286 22.63423316523287 38-39 21.061999406704242 27.889844754276673 28.265598734302383 22.7825571047167 40-41 21.427865124097696 25.496885197270842 28.938000593295758 24.137249085335707 42-43 20.641748244833384 26.075348561257787 28.73529120933452 24.54761198457431 44-45 19.489765648175617 25.818253732819148 30.040541876792247 24.651438742212996 46-47 20.167111638485117 25.595767823593395 30.47562543261149 23.761495105309997 48-49 19.578760011865914 25.813309601503015 30.534955008405024 24.072975378226047 50-51 18.238900425195293 26.846633046573714 29.837832492831012 25.07663403539998 52-53 17.927420152279243 26.49065559181252 30.282804311282508 25.29911994462573 54-55 17.23524176802136 26.456046672599626 30.045486008108373 26.26322555127064 56-57 17.798872738059924 26.15445466231583 29.87738554336003 26.169287056264213 58-59 17.82853752595669 26.15445466231583 29.783447048353608 26.233560763373877 60-61 18.55532482942747 25.991298328883616 28.834173835657072 26.61920300603184 62-63 17.858202313853454 25.838030258083656 28.769900128547416 27.533867299515475 64-65 19.074458617620884 25.882527439928804 27.904677148225055 27.138336794225253 66-67 19.499653910807872 25.734203500444973 26.90596262236725 27.860179966379906 68-69 19.094235142885395 25.29911994462573 26.391772965489963 29.21487194699891 70-71 20.53297735587857 25.80836547018689 26.114901611786813 27.54375556214773 72-73 20.770295659052703 25.590823692277265 24.517947196677543 29.120933451992485 74-75 21.605853851478297 25.818253732819148 24.443785226935628 28.132107188766938 76 21.536636013052508 24.12736082270345 24.493226540096906 29.842776624147138 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 1.0 14 1.0 15 1.0 16 1.0 17 0.0 18 1.5 19 1.5 20 1.5 21 3.0 22 3.0 23 4.5 24 10.0 25 14.0 26 23.5 27 34.0 28 44.0 29 53.0 30 64.5 31 79.0 32 113.5 33 145.0 34 159.0 35 222.0 36 281.0 37 291.0 38 334.0 39 434.5 40 554.0 41 640.5 42 665.0 43 667.0 44 693.0 45 755.0 46 793.0 47 796.5 48 763.0 49 698.0 50 670.0 51 607.0 52 511.5 53 436.0 54 393.0 55 346.5 56 260.0 57 194.0 58 168.0 59 133.5 60 80.5 61 51.0 62 40.0 63 28.0 64 16.0 65 15.0 66 13.5 67 13.0 68 9.0 69 5.0 70 4.5 71 4.0 72 2.5 73 1.5 74 2.5 75 3.0 76 1.5 77 1.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 1.0 86 1.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6130722831998417 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 10113.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.00207653515277 #Duplication Level Percentage of deduplicated Percentage of total 1 85.32284965451512 70.81973697221399 2 10.578984989278057 17.56155443488579 3 2.918751489158923 7.267873034707802 4 0.7981891827495831 2.650054385444477 5 0.28591851322373124 1.1865915158706615 6 0.05956635692161067 0.29664787896766537 7 0.02382654276864427 0.13843567685157718 8 0.011913271384322134 0.07910610105804411 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.019776525264511024 0.0 2 0.0 0.0 0.0 0.019776525264511024 0.0 3 0.0 0.0 0.0 0.019776525264511024 0.0 4 0.0 0.0 0.0 0.019776525264511024 0.0 5 0.0 0.0 0.0 0.019776525264511024 0.0 6 0.0 0.0 0.0 0.019776525264511024 0.0 7 0.0 0.0 0.0 0.019776525264511024 0.0 8 0.0 0.0 0.0 0.019776525264511024 0.0 9 0.0 0.0 0.0 0.019776525264511024 0.0 10 0.0 0.0 0.0 0.019776525264511024 0.0 11 0.0 0.0 0.0 0.029664787896766538 0.0 12 0.0 0.0 0.0 0.029664787896766538 0.0 13 0.0 0.0 0.0 0.029664787896766538 0.0 14 0.0 0.0 0.0 0.029664787896766538 0.0 15 0.0 0.0 0.0 0.029664787896766538 0.0 16 0.0 0.0 0.0 0.029664787896766538 0.0 17 0.0 0.0 0.0 0.029664787896766538 0.0 18 0.0 0.0 0.0 0.03955305052902205 0.0 19 0.0 0.0 0.0 0.03955305052902205 0.0 20 0.0 0.0 0.0 0.03955305052902205 0.0 21 0.0 0.0 0.0 0.03955305052902205 0.0 22 0.0 0.0 0.0 0.03955305052902205 0.0 23 0.0 0.0 0.0 0.03955305052902205 0.0 24 0.0 0.0 0.0 0.03955305052902205 0.0 25 0.0 0.0 0.0 0.03955305052902205 0.0 26 0.0 0.0 0.0 0.03955305052902205 0.0 27 0.0 0.0 0.0 0.059329575793533075 0.0 28 0.0 0.0 0.0 0.08899436369029962 0.0 29 0.0 0.0 0.0 0.11865915158706615 0.0 30 0.0 0.0 0.0 0.1582122021160882 0.0 31 0.0 0.0 0.0 0.19776525264511025 0.0 32 0.0 0.0 0.0 0.2472065658063878 0.0 33 0.0 0.0 0.0 0.3065361415999209 0.0 34 0.0 0.0 0.0 0.41530703055473156 0.0 35 0.0 0.0 0.0 0.5834074953030752 0.0 36 0.0 0.0 0.0 0.8701671116384851 0.0 37 0.0 0.0 0.0 1.2755858795609611 0.0 38 0.0 0.0 0.0 1.9380994759220804 0.0 39 0.0 0.0 0.0 2.7884900622960545 0.0 40 0.0 0.0 0.0 3.7179867497280727 0.0 41 0.0 0.0 0.0 4.795807376643924 0.0 42 0.0 0.0 0.0 5.952734104617819 0.0 43 0.0 0.0 0.0 7.22831998417878 0.0 44 0.0 0.0 0.0 8.37535844952042 0.0 45 0.0 0.0 0.0 9.65094432908138 0.0 46 0.0 0.0 0.0 11.015524572332641 0.0 47 0.0 0.0 0.0 12.083456936616237 0.0 48 0.0 0.0 0.0 12.953624048254722 0.0 49 0.0 0.0 0.0 14.100662513596362 0.0 50 0.0 0.0 0.0 15.326807079996044 0.0 51 0.0 0.0 0.0 16.48373380796994 0.0 52 0.0 0.0 0.0 17.60110748541481 0.0 53 0.0 0.0 0.0 18.72836942549194 0.0 54 0.0 0.0 0.0 19.776525264511026 0.0 55 0.0 0.0 0.0 20.93345199248492 0.0 56 0.0 0.0 0.0 21.872836942549196 0.0 57 0.0 0.0 0.0 22.930881044200532 0.0 58 0.0 0.0 0.0 24.068031246909918 0.0 59 0.0 0.0 0.0 24.928310095916146 0.0 60 0.0 0.0 0.0 25.927024621773953 0.0 61 0.0 0.0 0.0 26.79719173341244 0.0 62 0.0 0.0 0.0 27.85523583506378 0.0 63 0.0 0.0 0.0 29.09126866409572 0.0 64 0.0 0.0 0.0 30.089983189953525 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTTAA 20 0.006755182 52.500004 37 ACGAGCA 40 0.002308042 35.000004 69 CGAGCAG 40 0.002308042 35.000004 70 GCCGACG 115 9.170789E-4 18.26087 70 TGCCGAC 140 1.6455528E-4 17.5 69 CTGCCGA 145 2.1525304E-4 16.896551 68 GCTGCCG 150 2.789033E-4 16.333332 67 CGCTGCC 155 3.5817525E-4 15.806453 66 ACGCTGC 160 4.5616415E-4 15.312501 65 CTGACGC 160 4.5616415E-4 15.312501 62 GACGCTG 165 5.7643815E-4 14.848486 64 TGACGCT 165 5.7643815E-4 14.848486 63 TCTGACG 170 7.2309095E-4 14.411765 61 CATCTGA 190 0.0016769388 12.894736 59 ATCTGAC 190 0.0016769388 12.894736 60 ACATCTG 230 4.898212E-4 12.173914 58 CACATCT 250 9.878138E-4 11.2 57 >>END_MODULE