FastQCFastQC Report
Wed 25 May 2016
SRR1780532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT920.9097201621675072TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT920.9097201621675072No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG280.27687135370315435No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC180.17798872738059923No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC180.17798872738059923No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC150.14832393948383268No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC140.13843567685157718No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAA200.00675518252.50000459
GGAGCCT200.00675518252.50000413
TCTGCTT602.2755376E-740.83333253
GTCTTCT602.2755376E-740.83333249
CGTATGC602.2755376E-740.83333241
TCTTCTG602.2755376E-740.83333250
CTTCTGC602.2755376E-740.83333251
CGTCTTC556.0646453E-638.1818248
TTCTGCT654.2647116E-737.69230752
CTGCTTG654.2647116E-737.69230754
TTGAAAA654.2647116E-737.69230758
CTTGAAA654.2647116E-737.69230757
GTATGCC601.1017746E-535.042
TATGCCG601.1017746E-535.043
CCGTCTT601.1017746E-535.047
TGCTTGA707.622166E-735.055
GCTTGAA707.622166E-735.056
TCGTATG601.1017746E-535.040
GCCGTCT601.1017746E-535.046
TGAAAAA707.622166E-735.059