##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780532_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10113 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37506180164145 34.0 34.0 34.0 31.0 34.0 2 33.12429546128745 34.0 34.0 34.0 32.0 34.0 3 33.320181944032434 34.0 34.0 34.0 33.0 34.0 4 33.27133392662909 34.0 34.0 34.0 33.0 34.0 5 33.3103925640265 34.0 34.0 34.0 33.0 34.0 6 36.9425491941066 38.0 37.0 38.0 35.0 38.0 7 36.57371699792346 38.0 37.0 38.0 34.0 38.0 8 36.53129635123109 38.0 37.0 38.0 34.0 38.0 9 36.29664787896767 38.0 37.0 38.0 34.0 38.0 10-11 35.975131019479875 38.0 36.5 38.0 32.5 38.0 12-13 35.90843468802531 38.0 36.0 38.0 32.0 38.0 14-15 36.19707307426086 38.0 36.5 38.0 33.0 38.0 16-17 36.308019380994764 38.0 37.0 38.0 34.0 38.0 18-19 36.334223276970235 38.0 37.0 38.0 34.0 38.0 20-21 36.0083061406111 38.0 36.5 38.0 32.0 38.0 22-23 36.019677642638186 38.0 36.5 38.0 32.5 38.0 24-25 35.16696331454564 37.5 35.0 38.0 29.0 38.0 26-27 35.47414219321665 37.5 36.0 38.0 31.0 38.0 28-29 35.28201325027193 37.5 36.0 38.0 29.0 38.0 30-31 34.80801938099476 37.0 35.0 38.0 27.0 38.0 32-33 35.49470977949174 37.5 36.0 38.0 31.0 38.0 34-35 35.56298823296747 37.5 36.5 38.0 31.5 38.0 36-37 35.781024424008706 38.0 36.5 38.0 31.5 38.0 38-39 35.35612577870068 38.0 36.0 38.0 29.5 38.0 40-41 34.64308316028874 37.0 34.5 38.0 26.5 38.0 42-43 35.17576386828834 37.0 35.5 38.0 29.0 38.0 44-45 35.48600810837536 38.0 36.0 38.0 31.0 38.0 46-47 35.80421239988134 38.0 36.5 38.0 32.0 38.0 48-49 35.502027093839615 38.0 36.0 38.0 31.0 38.0 50-51 35.71942054780975 38.0 36.0 38.0 31.5 38.0 52-53 35.65361415999209 38.0 36.0 38.0 31.0 38.0 54-55 35.89093246316622 38.0 36.5 38.0 32.5 38.0 56-57 35.98566201918323 38.0 37.0 38.0 32.5 38.0 58-59 36.087857213487595 38.0 37.0 38.0 34.0 38.0 60-61 35.595322851774945 38.0 36.5 38.0 29.5 38.0 62-63 35.86902996143578 38.0 36.5 38.0 32.0 38.0 64-65 35.94605952734105 38.0 37.0 38.0 33.0 38.0 66-67 35.153169188173635 38.0 36.0 38.0 28.5 38.0 68-69 34.45649164441808 37.0 34.0 38.0 25.0 38.0 70-71 34.455255611589045 37.0 34.0 38.0 25.0 38.0 72-73 33.87417185800455 37.0 34.0 38.0 23.5 38.0 74-75 34.56076337387521 37.0 34.5 38.0 25.0 38.0 76 33.945614555522596 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.21193411301057807 1101 2 0.7871181292182214 1101 3 0.06871422897894064 1101 4 0.583975058797435 1101 5 0.7533005760405374 1101 6 -2.577038256683437 1101 7 -0.313711226054771 1101 8 0.5914913170998517 1101 9 0.3097383312560993 1101 10-11 -0.3093500535571394 1101 12-13 0.501434197370962 1101 14-15 1.427588204550844 1101 16-17 0.41048504664389895 1101 18-19 -0.3543662878547025 1101 20-21 -0.14124478644079375 1101 22-23 1.2004720440724483 1101 24-25 -1.0689935539296087 1101 26-27 1.4830312334762965 1101 28-29 2.504850909761686 1101 30-31 1.9258340764639001 1101 32-33 0.34744587611738353 1101 34-35 1.227749482524473 1101 36-37 -0.623040507589117 1101 38-39 0.08824359195548226 1101 40-41 1.849255265826102 1101 42-43 1.8556601289286832 1101 44-45 1.1593290506913192 1101 46-47 1.188868387943856 1101 48-49 -1.7670047412290302 1101 50-51 -0.6855581769697849 1101 52-53 -1.2289619857467287 1101 54-55 0.13272270721711266 1101 56-57 -0.34896261067424916 1101 58-59 0.12031946914911629 1101 60-61 -2.074936113802792 1101 62-63 0.23090829786814027 1101 64-65 -1.6326200024875988 1101 66-67 -1.7238214842077184 1101 68-69 0.7003746499807946 1101 70-71 -0.6050584502229484 1101 72-73 -0.18166082672966866 1101 74-75 0.3063114184665423 1101 76 1.3716662876020678 1102 1 -0.011934113010575231 1102 2 0.5537847958848943 1102 3 -0.3979524376877279 1102 4 0.6173083921307736 1102 5 0.7199672427072059 1102 6 0.7229617433165672 1102 7 -0.11371122605477524 1102 8 0.6581579837665217 1102 9 1.2097383312560979 1102 10-11 1.1406499464428634 1102 12-13 1.5681008640376248 1102 14-15 0.8109215378841839 1102 16-17 0.8938183799772332 1102 18-19 0.5123003788119576 1102 20-21 1.192088546892542 1102 22-23 1.0338053774057911 1102 24-25 -3.268993553929608 1102 26-27 -0.6003020998570392 1102 28-29 0.2715175764283515 1102 30-31 1.8591674097972302 1102 32-33 0.6141125427840421 1102 34-35 1.2444161491911387 1102 36-37 -3.773040507589119 1102 38-39 0.604910258622148 1102 40-41 2.1825885991594305 1102 42-43 1.4556601289286846 1102 44-45 1.159329050691312 1102 46-47 1.2055350546105217 1102 48-49 -2.7003380745623673 1102 50-51 -0.5022248436364549 1102 52-53 0.9377046809199427 1102 54-55 0.6327227072171127 1102 56-57 1.1510373893257437 1102 58-59 1.2536528024824491 1102 60-61 -5.724936113802784 1102 62-63 -3.202425035465197 1102 64-65 0.8173799975124041 1102 66-67 1.4761785157922844 1102 68-69 0.18370798331412175 1102 70-71 -1.5883917835562826 1102 72-73 -6.931660826729669 1102 74-75 -2.743688581533455 1102 76 0.43833295426873775 1103 1 0.9342197331432658 1103 2 0.5460924881925848 1103 3 0.5020475623122707 1103 4 0.7583340331564088 1103 5 0.44560826834823075 1103 6 0.46398738434220377 1103 7 1.1580836457400991 1103 8 0.7094400350485728 1103 9 0.6251229466407153 1103 10-11 0.7432140490069656 1103 12-13 0.6360495819863488 1103 14-15 -0.3288220518594045 1103 16-17 -0.024130337971485005 1103 18-19 0.08281319932478226 1103 20-21 -0.35662940182540837 1103 22-23 0.587651531251943 1103 24-25 -3.326685861621918 1103 26-27 0.37790302834809353 1103 28-29 -0.02591832100755198 1103 30-31 2.600193050822874 1103 32-33 1.3602663889378945 1103 34-35 0.12005717483216927 1103 36-37 -1.3935533281019374 1103 38-39 -1.13098717727528 1103 40-41 0.7274603940312261 1103 42-43 0.12360884687740281 1103 44-45 2.1439444353067003 1103 46-47 1.6145094135848836 1103 48-49 -0.8349534591777541 1103 50-51 0.9400828486712385 1103 52-53 0.9787303219455836 1103 54-55 0.5891329636273639 1103 56-57 0.6959091841975464 1103 58-59 -0.6399369411072939 1103 60-61 -3.1646797035463763 1103 62-63 0.003985220945061485 1103 64-65 0.4084056385380421 1103 66-67 0.035152874766637865 1103 68-69 0.615759265365412 1103 70-71 1.16801847285398 1103 72-73 -2.7496095446783855 1103 74-75 -1.148816786661655 1103 76 -2.3283337123979315 1104 1 -0.9021301914419517 1104 2 0.11849067823783344 1104 3 0.5706750132926643 1104 4 -0.8336720000260911 1104 5 -1.3349347180771112 1104 6 0.36217742959107113 1104 7 0.0019750484550300484 1104 8 0.618942297492012 1104 9 1.3528755861580635 1104 10-11 -0.15836966140027897 1104 12-13 0.7220224326650779 1104 14-15 0.6383725182763413 1104 16-17 0.16930857605566985 1104 18-19 0.3848493984198029 1104 20-21 0.3469905076768498 1104 22-23 1.14458969113128 1104 24-25 0.9761044852860792 1104 26-27 1.509501821711595 1104 28-29 2.6117136548597273 1104 30-31 1.4101478019540963 1104 32-33 1.2935243074899319 1104 34-35 -0.8075446351225821 1104 36-37 1.4710771394697062 1104 38-39 1.0843220233280348 1104 40-41 2.1384709521006044 1104 42-43 0.959581697556132 1104 44-45 1.8328584624560236 1104 46-47 1.6427899565713062 1104 48-49 0.5055442783788067 1104 50-51 0.8997359406772745 1104 52-53 0.421038014253277 1104 54-55 -0.14276748886132395 1104 56-57 -0.6381782969487588 1104 58-59 0.005613586796172854 1104 60-61 0.13584819992270525 1104 62-63 0.747574964534806 1104 64-65 0.0713015661398515 1104 66-67 -0.9610763861685001 1104 68-69 2.0984138656670694 1104 70-71 1.1812160595809758 1104 72-73 1.5075548595448396 1104 74-75 -0.5270219148667863 1104 76 0.04421530720991029 1105 1 0.6436214425449762 1105 2 0.5760070181071129 1105 3 0.8076031178678278 1105 4 0.4228639476863236 1105 5 0.5588561315960945 1105 6 0.9896284099832329 1105 7 0.8140665517230019 1105 8 0.04704687265540741 1105 9 0.6208494423672093 1105 10-11 0.32120550199841347 1105 12-13 0.6403230862598477 1105 14-15 0.427588204550851 1105 16-17 -0.12562606446721247 1105 18-19 0.7484114899230718 1105 20-21 0.9115329913369763 1105 22-23 0.39213871073912543 1105 24-25 1.1198953349592813 1105 26-27 1.5552534556985194 1105 28-29 1.35762868753946 1105 30-31 2.014722965352796 1105 32-33 0.014112542784047832 1105 34-35 -0.3083616285866384 1105 36-37 0.06584838129976589 1105 38-39 0.12435470306659369 1105 40-41 1.7936997102705448 1105 42-43 1.2945490178175731 1105 44-45 1.717662384024642 1105 46-47 1.366646165721633 1105 48-49 0.966328592104297 1105 50-51 1.7061084896968879 1105 52-53 1.1432602364754985 1105 54-55 0.48550048499488696 1105 56-57 0.7760373893257437 1105 58-59 1.1036528024824435 1105 60-61 1.3972861084194363 1105 62-63 -0.7802028132429726 1105 64-65 0.6007133308457355 1105 66-67 1.1067340713478373 1105 68-69 0.055930205536348865 1105 70-71 1.6477193275548316 1105 72-73 1.09333917327033 1105 74-75 -0.47146635931122916 1105 76 1.1994440653798506 1106 1 -0.901181424838537 1106 2 0.5312041507236032 1106 3 0.8353808956456064 1106 4 0.23558796202324572 1106 5 -0.05315103686268685 1106 6 0.8337144314886089 1106 7 -1.9287649894956331 1106 8 0.7119214246267376 1106 9 0.5473727398582469 1106 10-11 0.6997897313890888 1106 12-13 0.9772406489838659 1106 14-15 -0.8412289997502285 1106 16-17 -0.12338592109803415 1106 18-19 0.12251543257540476 1106 20-21 0.8232713425914611 1106 22-23 1.1327301085885821 1106 24-25 -1.0727569947898274 1106 26-27 0.7281925237988816 1106 28-29 1.8123777914821204 1106 30-31 1.0989523560337986 1106 32-33 0.11088673633243218 1106 34-35 1.0890398051051235 1106 36-37 1.5721207827334567 1106 38-39 0.09523283926731807 1106 40-41 0.077749889482007 1106 42-43 1.0454450751652473 1106 44-45 1.614167760368737 1106 46-47 1.5996210761158949 1106 48-49 1.8238554738247288 1106 50-51 0.3337966617398962 1106 52-53 0.6065218852210208 1106 54-55 0.3596044276472199 1106 56-57 0.3580266366375753 1106 58-59 1.4638678562458836 1106 60-61 1.559472488347751 1106 62-63 1.247574964534806 1106 64-65 -1.3992866691542645 1106 66-67 -0.5711333121647044 1106 68-69 2.236933789765743 1106 70-71 -0.06688640721218775 1106 72-73 0.8016725066036656 1106 74-75 1.6100748593267653 1106 76 1.2093006962042168 1107 1 0.9740307992701247 1107 2 -0.9093730988519511 1107 3 0.5985387903824488 1107 4 0.4155540061658556 1107 5 -0.009857318696305128 1107 6 0.6299792871762122 1107 7 1.3827800020154015 1107 8 -1.4909648232510264 1107 9 -1.7709634231298637 1107 10-11 0.5924043324077672 1107 12-13 -0.024881592102723005 1107 14-15 -0.23469249720353957 1107 16-17 -0.06056758493504333 1107 18-19 0.928967045478629 1107 20-21 1.1008604767171022 1107 22-23 0.3636299388092894 1107 24-25 0.2222345162458339 1107 26-27 0.043557549265777595 1107 28-29 1.1039737167792296 1107 30-31 0.31881653260425225 1107 32-33 1.106217805941938 1107 34-35 -0.12488209642289405 1107 36-37 0.7427489660950926 1107 38-39 0.6601734165168907 1107 40-41 -0.4358324534721447 1107 42-43 0.5416250412093859 1107 44-45 0.522486945428156 1107 46-47 1.3300964581193 1107 48-49 0.615451399121838 1107 50-51 1.427599717767059 1107 52-53 0.8026169616217018 1107 54-55 0.8897402510767591 1107 56-57 1.2650724770450452 1107 58-59 1.0334773638859573 1107 60-61 1.2627831844428243 1107 62-63 1.273890754008491 1107 64-65 1.1928185940036329 1107 66-67 1.483196059651931 1107 68-69 1.4784448254193876 1107 70-71 1.0782748831103888 1107 72-73 1.3937777697615559 1107 74-75 -0.04017980960362877 1107 76 -0.18798283520494863 1108 1 -0.21748966856613094 1108 2 -0.9795485374484443 1108 3 -0.05350799324327937 1108 4 0.6728639476863236 1108 5 0.3921894649294231 1108 6 0.5174061877610114 1108 7 0.28628877394522334 1108 8 -1.0918420162334783 1108 9 -0.795817224299455 1108 10-11 0.598983279776192 1108 12-13 1.556989752926519 1108 14-15 0.7609215378841867 1108 16-17 -0.2645149533560982 1108 18-19 0.3178559343675147 1108 20-21 1.3420885468925405 1108 22-23 0.350472044072454 1108 24-25 1.7171175571815027 1108 26-27 0.4580312334762979 1108 28-29 0.2326286875394601 1108 30-31 -0.38805481242498985 1108 32-33 0.9724458761173764 1108 34-35 -0.1000282952533027 1108 36-37 1.871403936855323 1108 38-39 1.9437991475110366 1108 40-41 1.140921932492759 1108 42-43 -0.5248954266268697 1108 44-45 1.2732179395801992 1108 46-47 1.8805350546105188 1108 48-49 1.0635508143265184 1108 50-51 0.6783307119191022 1108 52-53 -0.6067397635245015 1108 54-55 -1.6256106261162202 1108 56-57 -0.237851499563142 1108 58-59 1.1730972469268863 1108 60-61 0.8556194417527649 1108 62-63 1.1642416312014703 1108 64-65 1.3090466641790641 1108 66-67 0.6761785157922873 1108 68-69 1.4309302055363489 1108 70-71 -1.0328362280007255 1108 72-73 -1.1844386045074486 1108 74-75 -0.8603552482001149 1108 76 2.0605551764909578 1109 1 0.1356849346084701 1109 2 0.5109276530277498 1109 3 0.40680946707417576 1109 4 0.3554036302260073 1109 5 0.2675862903262498 1109 6 0.6134379337927527 1109 7 -1.6375207498643007 1109 8 0.6391103647189027 1109 9 1.3049764264941928 1109 10-11 1.059697565490481 1109 12-13 1.3657199116566758 1109 14-15 1.2513977283603737 1109 16-17 1.2319136180724684 1109 18-19 1.1146813311929122 1109 20-21 1.6587552135592034 1109 22-23 1.0290434726438846 1109 24-25 -0.6856602205962758 1109 26-27 0.5687455191905855 1109 28-29 -0.7046728997621301 1109 30-31 1.1210721717019965 1109 32-33 1.342683971355477 1109 34-35 0.7563209110958979 1109 36-37 1.3412452066965912 1109 38-39 2.0906245443364426 1109 40-41 0.7611600277308597 1109 42-43 0.41042203369059393 1109 44-45 0.802186193548458 1109 46-47 1.0650588641343361 1109 48-49 1.5782333540090576 1109 50-51 0.45729896588736096 1109 52-53 0.7853237285389909 1109 54-55 -1.133943959449553 1109 56-57 0.4819897702781262 1109 58-59 0.8417480405776843 1109 60-61 0.5774448385781596 1109 62-63 0.6047178216776672 1109 64-65 1.6292847594171604 1109 66-67 1.7952261348399006 1109 68-69 -0.30914915954301136 1109 70-71 0.06398916882466921 1109 72-73 -1.512613207682051 1109 74-75 -0.4913076291525016 1109 76 1.0240472399830196 1110 1 -2.2928864939629605 1110 2 -1.9462152041151093 1110 3 -0.16461910435439364 1110 4 0.12683220165457953 1110 5 -0.01812799538803489 1110 6 -0.729419209064389 1110 7 0.5624792501357021 1110 8 0.43911036471889986 1110 9 1.219262140779911 1110 10-11 -0.2688738630809553 1110 12-13 1.3228627687995314 1110 14-15 1.2942548712175181 1110 16-17 1.2604850466439004 1110 18-19 0.9861099026214859 1110 20-21 1.8301837849877742 1110 22-23 -0.6852422416418307 1110 24-25 -0.8428030777391342 1110 26-27 1.7544598049048687 1110 28-29 0.8238985288093019 1110 30-31 -0.2503563997265772 1110 32-33 1.1712553999269062 1110 34-35 1.7420351968101926 1110 36-37 2.1126737781251634 1110 38-39 1.1763388300507174 1110 40-41 0.13258859915943333 1110 42-43 0.738993462262016 1110 44-45 1.9164719078341719 1110 46-47 1.8936302927057653 1110 48-49 2.235376211151916 1110 50-51 0.8430132516016471 1110 52-53 1.085323728538988 1110 54-55 0.4231988976933039 1110 56-57 1.1677040559924095 1110 58-59 1.127462326291976 1110 60-61 1.320301981435307 1110 62-63 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-1.3996253500192068 2116 70-71 1.011608216443726 2116 72-73 -1.4316608267296687 2116 74-75 -2.3436885815334527 2116 76 -3.1950003790645987 2117 1 0.9213992203227548 2117 2 0.3537847958848914 2117 3 0.6210951813598911 2117 4 -0.858882084059708 2117 5 0.6104434331833914 2117 6 -0.8437049233501028 2117 7 1.119622107278559 2117 8 -1.9608896352810987 2117 9 0.604976426494197 2117 10-11 0.6168404226333308 2117 12-13 -3.5342800883433227 2117 14-15 -1.227173700211054 2117 16-17 -2.818086381927529 2117 18-19 -4.463890097378513 2117 20-21 -0.7412447864407952 2117 22-23 -2.006670813070407 2117 24-25 -3.042803077739137 2117 26-27 -2.059825909380848 2117 28-29 -4.211815756904983 2117 30-31 0.3996436002734285 2117 32-33 -2.271601742930237 2117 34-35 0.5348923396673371 2117 36-37 0.17695949241088016 2117 38-39 0.4477674014792896 2117 40-41 -1.8531256865548542 2117 42-43 -0.8324351091665534 2117 44-45 -2.3478138064515477 2117 46-47 -3.5063697072942404 2117 48-49 -2.093195217419506 2117 50-51 -3.5855581769697835 2117 52-53 -0.22181912860386177 2117 54-55 -0.048229673735271206 2117 56-57 0.49627548456384574 2117 58-59 -2.5868233879937392 2117 60-61 -1.4439837328504055 2117 62-63 -0.4309964640366246 2117 64-65 -1.2564295262971257 2117 66-67 -2.490488150874384 2117 68-69 -1.6662920166858726 2117 70-71 -0.9217251168896112 2117 72-73 2.515958220889381 2117 74-75 2.4729780851332137 2117 76 1.124047239983021 2118 1 -0.2928864939629605 2118 2 0.6394990815991761 2118 3 -0.6646191043543936 2118 4 -1.0731677983454162 2118 5 0.39615771889768325 2118 6 -1.4151334947786722 2118 7 -0.8089493212928716 2118 8 0.2533960790046166 2118 9 -2.752166430648664 2118 10-11 -1.1331595773666692 2118 12-13 -1.7128515169147533 2118 14-15 0.0585405855032306 2118 16-17 -2.7466578104989523 2118 18-19 -0.32103295452137104 2118 20-21 -0.8126733578693646 2118 22-23 0.6361863297867387 2118 24-25 0.17148263654658535 2118 26-27 -3.666968766523702 2118 28-29 -0.8546728997621216 2118 30-31 -2.6360706854408633 2118 32-33 -1.2358874572159522 2118 34-35 -0.17939337461838534 2118 36-37 -8.00161193616055 2118 38-39 -1.337946884234995 2118 40-41 -1.138839972269139 2118 42-43 0.7747077479763078 2118 44-45 -4.2406709493086865 2118 46-47 0.49363029270575964 2118 48-49 0.9068047825804939 2118 50-51 -2.406986748398353 2118 52-53 -4.078961985746723 2118 54-55 -3.0125153880209865 2118 56-57 0.13913262742098453 2118 58-59 -0.08682338799374634 2118 60-61 -2.515412304278975 2118 62-63 -3.1809964640366246 2118 64-65 0.13642761656002733 2118 66-67 -0.27620243658866883 2118 68-69 -2.130577730971588 2118 70-71 -2.6717251168896112 2118 72-73 -0.9483274933963344 2118 74-75 1.187263799418929 2118 76 2.1954758114115904 2119 1 1.4213992203227548 2119 2 -1.5462152041151072 2119 3 -1.864619104354393 2119 4 -0.916024941202565 2119 5 -1.446699423959462 2119 6 -1.0437049233501057 2119 7 -0.7803778927214395 2119 8 -0.9751753495668112 2119 9 -1.0235950020772364 2119 10-11 -1.2260167202238108 2119 12-13 -2.198565802629041 2119 14-15 -1.1557451287824847 2119 16-17 -1.839514953356101 2119 18-19 -1.1710329545213654 2119 20-21 -3.491244786440795 2119 22-23 -1.149527955927546 2119 24-25 1.0643397794037242 2119 26-27 -0.21696876652369923 2119 28-29 -0.36181575690498846 2119 30-31 -0.007499256869429871 2119 32-33 0.26411254278404783 2119 34-35 1.477749482524473 2119 36-37 -0.5230405075891227 2119 38-39 -0.2950897413778506 2119 40-41 0.2825885991594319 2119 42-43 -3.211006537737987 2119 44-45 -2.640670949308692 2119 46-47 -1.0777982787228098 2119 48-49 -0.10033807456237298 2119 50-51 1.6644418230302165 2119 52-53 0.5210380142532784 2119 54-55 0.1660560405504441 2119 56-57 -1.0822959440075905 2119 58-59 -1.3796805308508837 2119 60-61 -1.1582694471361208 2119 62-63 -3.1524250354651926 2119 64-65 0.7507133308457412 2119 66-67 -0.09048815087437845 2119 68-69 -1.016292016685874 2119 70-71 1.2282748831103873 2119 72-73 0.7016725066036713 2119 74-75 -2.0270219148667863 2119 76 0.33833295426873633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 3.0 24 6.0 25 13.0 26 28.0 27 45.0 28 96.0 29 155.0 30 248.0 31 331.0 32 502.0 33 780.0 34 1109.0 35 1587.0 36 2352.0 37 2857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.48640363888065 22.13982003362009 11.885691683971126 28.488084643528133 2 18.57015722337585 19.578760011865914 36.3195886482745 25.53149411648373 3 17.551666172253537 24.68110353010976 30.920597251062986 26.846633046573714 4 13.032730149312766 19.430436072382083 37.931375457332145 29.605458320973003 5 12.36032829031939 36.33936517353901 35.61752200138436 15.682784534757243 6 26.34233165232869 39.15752002373183 18.036191041234055 16.46395728270543 7 26.322555127064174 30.97992682685652 23.504400276871355 19.19311776920795 8 22.021160882033026 35.43953327400376 20.775239790368833 21.764066053594384 9 21.833283892020173 17.067141303273015 22.466132700484525 38.63344210422229 10-11 22.930881044200536 25.852862652032037 28.240878077721742 22.975378226045684 12-13 23.128646296845645 23.766439236626123 27.01473351132206 26.090180955206172 14-15 20.523089093246316 24.78987441906457 25.002472065658065 29.684564422031052 16-17 21.769010184910513 28.522693562741026 26.19400771284485 23.514288539503607 18-19 21.34875902303965 28.428755067734603 26.40166122812222 23.82082468110353 20-21 20.29565905270444 27.430040541876792 27.177889844754276 25.096410560664488 22-23 21.55146840700089 28.760011865915157 26.500543854444775 23.18797587263918 24-25 21.828339760704043 29.654899634134285 26.184119450212595 22.332641154949076 26-27 21.892613467813703 29.34341936121823 26.856521309205974 21.90744586176209 28-29 22.772668842084446 27.766241471373483 28.00850390586374 21.452585780678334 30-31 23.830712943735787 26.732918026302777 27.78601799663799 21.650351033323446 32-33 22.569959458123208 27.21249876396717 28.764955997231283 21.452585780678334 34-35 22.03599327598141 27.63274992583803 29.521408088598832 20.809848709581726 36-37 22.27825571047167 27.667358845050927 28.73529120933452 21.319094235142884 38-39 21.269652921981606 27.425096410560663 27.756353208741224 23.548897458716503 40-41 20.191832295065755 25.946801147038467 30.29763670523089 23.563729852664885 42-43 21.294373578562247 25.941857015722334 30.040541876792247 22.723227528923168 44-45 19.504598042123998 26.807079996044695 30.094927321269655 23.593394640561655 46-47 21.047167012755857 24.250964105606645 30.549787402353406 24.15208147928409 48-49 19.672698506872344 24.364679125877583 30.81182636210818 25.150796005141896 50-51 18.332838920201723 25.21506971225156 31.637496291901513 24.81459507564521 52-53 18.05102343518244 25.24967863146445 33.03668545436567 23.66261247898744 54-55 16.098091565311975 25.936912884406212 32.49777514090774 25.467220409374075 56-57 18.085632354395333 25.402946702264412 30.99970335212103 25.511717591219224 58-59 17.215465242756846 26.688420844457628 30.554731533669532 25.54138237911599 60-61 18.219123899930782 27.56847621872837 28.829229704340946 25.383170176999904 62-63 16.933649757737566 28.908335805398995 27.76129734005735 26.396717096806093 64-65 17.309403737763272 29.931770987837435 26.381884702857704 26.376940571541578 66-67 16.78532581825373 29.837832492831012 25.71937110649659 27.657470582418668 68-69 16.77049342430535 29.872441412043905 25.012360328290317 28.344704835360428 70-71 18.1152971422921 28.89350341145061 24.868980520122612 28.12221892613468 72-73 19.029961435775732 28.018392168496 24.765153762483934 28.186492633244338 74-75 20.22644121427865 26.777415208147932 24.55255611589044 28.443587461682984 76 20.21160882033027 26.757638682883417 24.98763967170968 28.043112825076633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 1.5 17 3.0 18 1.5 19 2.0 20 4.0 21 4.0 22 4.0 23 7.5 24 12.5 25 14.0 26 21.5 27 31.0 28 46.0 29 59.0 30 68.0 31 88.5 32 136.0 33 172.0 34 180.0 35 219.5 36 265.0 37 279.0 38 325.0 39 451.5 40 590.5 41 697.0 42 745.0 43 780.0 44 845.0 45 842.0 46 809.0 47 823.5 48 804.5 49 679.5 50 588.0 51 553.5 52 467.0 53 362.0 54 309.0 55 287.0 56 211.0 57 134.5 58 112.0 59 89.0 60 48.0 61 21.0 62 12.0 63 7.5 64 2.0 65 1.0 66 1.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 10113.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.80727776129734 #Duplication Level Percentage of deduplicated Percentage of total 1 77.29186472905066 56.27410264016612 2 15.550726606002987 22.644121427865123 3 4.617683009642809 10.086027884900622 4 1.4667934265924216 4.271729457134382 5 0.6247453483634388 2.274300405418768 6 0.20372130924894744 0.8899436369029962 7 0.10865136493277197 0.5537427074063087 8 0.013581420616596496 0.07910610105804411 9 0.013581420616596496 0.08899436369029962 >10 0.08148852369957897 1.0184910511223177 >50 0.027162841233192993 1.8194403243350143 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT 92 0.9097201621675072 TruSeq Adapter, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 92 0.9097201621675072 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 28 0.27687135370315435 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 18 0.17798872738059923 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 18 0.17798872738059923 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 15 0.14832393948383268 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 14 0.13843567685157718 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.019776525264511024 0.0 2 0.0 0.0 0.0 0.019776525264511024 0.0 3 0.0 0.0 0.0 0.019776525264511024 0.0 4 0.0 0.0 0.0 0.019776525264511024 0.0 5 0.0 0.0 0.0 0.019776525264511024 0.0 6 0.0 0.0 0.0 0.019776525264511024 0.0 7 0.0 0.0 0.0 0.019776525264511024 0.0 8 0.0 0.0 0.0 0.019776525264511024 0.0 9 0.0 0.0 0.0 0.019776525264511024 0.0 10 0.0 0.0 0.0 0.019776525264511024 0.0 11 0.0 0.0 0.0 0.029664787896766538 0.0 12 0.0 0.0 0.0 0.029664787896766538 0.0 13 0.0 0.0 0.0 0.029664787896766538 0.0 14 0.0 0.0 0.0 0.029664787896766538 0.0 15 0.0 0.0 0.0 0.029664787896766538 0.0 16 0.0 0.0 0.0 0.029664787896766538 0.0 17 0.0 0.0 0.0 0.029664787896766538 0.0 18 0.0 0.0 0.0 0.03955305052902205 0.0 19 0.0 0.0 0.0 0.03955305052902205 0.0 20 0.0 0.0 0.0 0.03955305052902205 0.0 21 0.0 0.0 0.0 0.03955305052902205 0.0 22 0.0 0.0 0.0 0.03955305052902205 0.0 23 0.0 0.0 0.0 0.03955305052902205 0.0 24 0.0 0.0 0.0 0.03955305052902205 0.0 25 0.0 0.0 0.0 0.03955305052902205 0.0 26 0.0 0.0 0.0 0.03955305052902205 0.0 27 0.0 0.0 0.0 0.059329575793533075 0.0 28 0.0 0.0 0.0 0.0791061010580441 0.0 29 0.0 0.0 0.0 0.09888262632255512 0.0 30 0.0 0.0 0.0 0.13843567685157718 0.0 31 0.0 0.0 0.0 0.18787699001285474 0.0 32 0.0 0.0 0.0 0.25709482843864334 0.0 33 0.0 0.0 0.0 0.32631266686443194 0.0 34 0.0 0.0 0.0 0.4449718184514981 0.0 35 0.0 0.0 0.0 0.6427370710966084 0.0 36 0.0 0.0 0.0 0.9097201621675072 0.0 37 0.0 0.0 0.0 1.3349154553544942 0.0 38 0.0 0.0 0.0 2.0172055769801247 0.0 39 0.0 0.0 0.0 2.917037476515376 0.0 40 0.0 0.0 0.0 4.0047463660634826 0.0 41 0.0 0.0 0.0 5.141896568772867 0.0 42 0.0 0.0 0.0 6.358152872540295 0.0 43 0.0 0.0 0.0 7.693068327894789 0.0 44 0.0 0.0 0.0 8.87965984376545 0.0 45 0.0 0.0 0.0 10.20468703648769 0.0 46 0.0 0.0 0.0 11.628596855532482 0.0 47 0.0 0.0 0.0 12.84485315929991 0.0 48 0.0 0.0 0.0 13.764461584099674 0.0 49 0.0 0.0 0.0 15.000494413131612 0.0 50 0.0 0.0 0.0 16.365074656382873 0.0 51 0.0 0.0 0.0 17.610995748047067 0.0 52 0.0 0.0 0.0 18.81736378918224 0.0 53 0.0 0.0 0.0 19.96440225452388 0.0 54 0.0 0.0 0.0 21.150993770394543 0.0 55 0.0 0.0 0.0 22.357361811529714 0.0 56 0.0 0.0 0.0 23.395629387916543 0.0 57 0.0 0.0 0.0 24.50311480272916 0.0 58 0.0 0.0 0.0 25.719371106496588 0.0 59 0.0 0.0 0.0 26.57964995550282 0.0 60 0.0 0.0 0.0 27.617917531889645 0.0 61 0.0 0.0 0.0 28.547414219321666 0.0 62 0.0 0.0 0.0 29.625234846237515 0.0 63 0.0 0.0 0.0 30.8513794126372 0.0 64 0.0 0.0 0.0 31.88964698902403 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAACAA 20 0.006755182 52.500004 59 GGAGCCT 20 0.006755182 52.500004 13 TCTGCTT 60 2.2755376E-7 40.833332 53 GTCTTCT 60 2.2755376E-7 40.833332 49 CGTATGC 60 2.2755376E-7 40.833332 41 TCTTCTG 60 2.2755376E-7 40.833332 50 CTTCTGC 60 2.2755376E-7 40.833332 51 CGTCTTC 55 6.0646453E-6 38.18182 48 TTCTGCT 65 4.2647116E-7 37.692307 52 CTGCTTG 65 4.2647116E-7 37.692307 54 TTGAAAA 65 4.2647116E-7 37.692307 58 CTTGAAA 65 4.2647116E-7 37.692307 57 GTATGCC 60 1.1017746E-5 35.0 42 TATGCCG 60 1.1017746E-5 35.0 43 CCGTCTT 60 1.1017746E-5 35.0 47 TGCTTGA 70 7.622166E-7 35.0 55 GCTTGAA 70 7.622166E-7 35.0 56 TCGTATG 60 1.1017746E-5 35.0 40 GCCGTCT 60 1.1017746E-5 35.0 46 TGAAAAA 70 7.622166E-7 35.0 59 >>END_MODULE