FastQCFastQC Report
Wed 25 May 2016
SRR1780531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135675
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC207.84679E-443.98303228
AGACTAT207.875359E-443.9506076
TGATACT250.002349896635.18642429
GATTGAT250.002349896635.18642426
TCTGTAT250.002358419635.1604848
CTCGTGG300.00568650329.376216
CATGACA300.005748431729.31120941
ATATCTG300.005748431729.31120943
GATGTGC300.005758804729.3004066
CCGTGCG300.005758804729.3004069
TATTAGA508.3515246E-526.3703652
CAGATGT450.001400969124.4170064
GTCGGTT551.5757218E-424.01732322
GAAGTAA551.5915927E-423.98189732
AAGAGAG602.847791E-422.00775325
GAGATTG500.002563486822.00775125
GTTAAGA500.00257450521.99151626
TTGAACC602.862055E-421.99151640
TGCTCGG500.00257450521.99151610
GAATTGA602.862055E-421.99151637
Produced by FastQC (version 0.11.5)