FastQCFastQC Report
Wed 25 May 2016
SRR1780531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135675
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT23851.7578772802653397No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT8370.6169154228855721TruSeq Adapter, Index 18 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG7690.5667956513727658No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC2920.21522019531969783No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC1670.12308826239174497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGTC207.8322436E-443.9996341
ATAGAGT207.8322436E-443.999636
TCAATGC207.8322436E-443.9996315
GTCAATG207.8322436E-443.9996314
CCGTCTT950.041.6838644
GCCGTCT1000.039.59966743
ACAGTTG250.002345557335.19970325
GGAGCAA250.002345557335.19970334
CACATGT250.002345557335.19970328
ATGCTCT250.002345557335.19970318
CTGAAAT250.002345557335.19970323
AATGCTC250.002345557335.19970317
TCAATTA250.002345557335.19970342
CTATACA401.80379E-533.0118941
GTACCTA300.005717199829.3439041
TGGAGCA300.00572751729.33308833
TCTGAAC300.00572751729.33308813
TACCCGC300.00572751729.33308810
GCGACAG300.00572751729.33308835
ACACATG300.00572751729.33308827
Produced by FastQC (version 0.11.5)