FastQCFastQC Report
Wed 25 May 2016
SRR1780530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19075
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG150.002192355569.9921346
TCGGCTT150.002192355569.9921324
CAATCAA150.002192355569.992139
AAGACGC150.002192355569.9921315
GACGCCC150.002192355569.9921317
AGACGCC150.002192355569.9921316
CAAAGGT150.002192355569.9921361
CGGCTTA150.002192355569.9921325
TCTCTTC150.002192355569.9921334
CCCTCGG150.002192355569.9921321
CTTCTCT150.002192355569.9921332
AATGACA150.002192355569.9921352
ACTACAC207.734811E-569.992133
TTACTAC200.006639425652.910711
GCCCTCG200.00685112352.49409520
TAAATTA200.00685112352.49409522
ACGCCCT200.00685112352.49409518
TTACTTC200.00685112352.49409529
CGCCCTC200.00685112352.49409519
ATGACAT200.00685112352.49409553
Produced by FastQC (version 0.11.5)