##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780530_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19075 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.79035386631717 34.0 31.0 34.0 12.0 34.0 2 31.361834862385322 34.0 32.0 34.0 23.0 34.0 3 31.48791612057667 34.0 32.0 34.0 23.0 34.0 4 32.15716906946265 34.0 34.0 34.0 27.0 34.0 5 32.193289646133685 34.0 34.0 34.0 27.0 34.0 6 33.9707994757536 37.0 34.0 38.0 24.0 38.0 7 32.80288335517693 37.0 31.0 38.0 21.0 38.0 8 32.6959370904325 37.0 32.0 38.0 21.0 38.0 9 34.01399737876802 37.0 34.0 38.0 24.0 38.0 10-11 34.45669724770642 37.0 34.0 38.0 26.0 38.0 12-13 34.83307994757536 38.0 35.5 38.0 26.5 38.0 14-15 34.72348623853211 38.0 35.0 38.0 26.5 38.0 16-17 34.6205242463958 38.0 35.0 38.0 26.0 38.0 18-19 34.54094364351245 38.0 35.0 38.0 25.0 38.0 20-21 34.59454783748362 38.0 35.0 38.0 26.0 38.0 22-23 34.63402359108781 37.5 35.0 38.0 25.5 38.0 24-25 35.099868938401045 38.0 36.0 38.0 27.5 38.0 26-27 35.125268676277855 38.0 36.0 38.0 27.0 38.0 28-29 35.372110091743124 38.0 36.5 38.0 31.0 38.0 30-31 35.27612057667103 38.0 36.0 38.0 28.5 38.0 32-33 34.85032765399738 38.0 36.0 38.0 26.5 38.0 34-35 34.97389252948886 38.0 36.0 38.0 27.0 38.0 36-37 34.928073394495414 38.0 36.0 38.0 27.0 38.0 38-39 35.4343119266055 38.0 36.5 38.0 31.0 38.0 40-41 34.852686762778504 38.0 36.0 38.0 27.0 38.0 42-43 35.20348623853211 38.0 36.0 38.0 27.0 38.0 44-45 34.23685452162516 37.0 34.0 38.0 24.5 38.0 46-47 34.9097247706422 37.0 36.0 38.0 27.0 38.0 48-49 34.70183486238532 37.0 35.5 38.0 26.5 38.0 50-51 34.58254259501966 37.0 34.5 38.0 26.0 38.0 52-53 34.475203145478375 37.0 34.5 38.0 25.5 38.0 54-55 34.25218872870249 37.0 34.0 38.0 24.5 38.0 56-57 34.329567496723456 37.0 34.5 38.0 25.0 38.0 58-59 34.21415465268676 37.0 34.5 38.0 25.0 38.0 60-61 34.22807339449541 37.0 34.5 38.0 24.5 38.0 62-63 34.34070773263434 37.0 34.0 38.0 25.0 38.0 64-65 34.43824377457405 37.0 34.5 38.0 25.5 38.0 66-67 34.36694626474443 37.0 34.0 38.0 25.0 38.0 68-69 33.808571428571426 37.0 34.0 38.0 23.5 38.0 70-71 33.856068152031455 37.0 34.0 38.0 24.0 38.0 72-73 33.96112712975098 37.0 34.0 38.0 24.0 38.0 74-75 33.58361730013106 37.0 33.0 38.0 23.0 38.0 76 32.845399737876804 37.0 31.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.473284409894884 1101 2 -0.9887238729382766 1101 3 -0.12100559882461326 1101 4 -1.8256383640149814 1101 5 -1.2739473494088145 1101 6 -2.690087542964619 1101 7 -1.931763321210763 1101 8 -2.920270613463696 1101 9 -1.4520732290807032 1101 10-11 -0.6362806667315866 1101 12-13 -1.0280612540669694 1101 14-15 -0.08413016376407967 1101 16-17 -1.132753842357559 1101 18-19 0.21693669404839966 1101 20-21 -0.4527890389622726 1101 22-23 -0.9277584418155342 1101 24-25 -0.09316262923688612 1101 26-27 -1.1364508320214455 1101 28-29 -0.14107426278780366 1101 30-31 -2.1860481800298572 1101 32-33 -1.0100157052672571 1101 34-35 -1.197256003088107 1101 36-37 0.23753633456977497 1101 38-39 -0.9300397413158521 1101 40-41 0.22180440929768963 1101 42-43 -0.4546183983823582 1101 44-45 0.08454111415348109 1101 46-47 0.5615804596658194 1101 48-49 -0.807598753556384 1101 50-51 -1.7664667588820464 1101 52-53 -0.2769029599649002 1101 54-55 -1.4129891264496308 1101 56-57 -1.4317954256799155 1101 58-59 -0.40550495625396366 1101 60-61 0.12124794941722428 1101 62-63 -2.2031821138420327 1101 64-65 0.1921482261852674 1101 66-67 -1.0188207744941877 1101 68-69 1.4590802858166185 1101 70-71 1.276784172822488 1101 72-73 0.7929173379467755 1101 74-75 -1.6405684995652692 1101 76 -3.522910298671995 1102 1 0.5007130838394502 1102 2 -1.323310339103692 1102 3 -0.02451437075443863 1102 4 -0.7307135519849055 1102 5 -0.22068920404540648 1102 6 -0.03939831990697229 1102 7 1.1948030948293393 1102 8 1.328476253704224 1102 9 0.7042550917213006 1102 10-11 -0.6394134988117912 1102 12-13 -4.946450978377747 1102 14-15 -2.1033970810573095 1102 16-17 -3.514019506517961 1102 18-19 -0.40978636359570686 1102 20-21 0.17675356755401594 1102 22-23 -1.5286356347979861 1102 24-25 -0.1271538573070643 1102 26-27 -5.282440806958782 1102 28-29 -1.8307672452439405 1102 30-31 -1.0203213629872536 1102 32-33 -8.107760066169512 1102 34-35 -1.5902697875492606 1102 36-37 -0.26684963034250586 1102 38-39 -0.1330472601128463 1102 40-41 0.39285704087663476 1102 42-43 0.1210394963544843 1102 44-45 -6.6302145249442646 1102 46-47 -1.132028562890575 1102 48-49 0.9845378379223177 1102 50-51 -6.036673525799337 1102 52-53 -1.914121005077675 1102 54-55 -0.012299903391983946 1102 56-57 -2.004633771544576 1102 58-59 -0.06715909159230193 1102 60-61 0.22102865117160775 1102 62-63 -4.610450284268097 1102 64-65 -1.1554394931129792 1102 66-67 0.2358784736261086 1102 68-69 -8.1615337492711 1102 70-71 -0.9337421429669917 1102 72-73 0.32628199960091564 1102 74-75 -8.535618624878548 1102 76 -2.303612053057961 1103 1 0.4194756592921678 1103 2 0.6213576689446008 1103 3 0.024781403893449294 1103 4 0.2529383594839061 1103 5 0.5745824257332686 1103 6 -2.0300924848969117 1103 7 0.7833342819520723 1103 8 2.304834402597585 1103 9 1.427343622908424 1103 10-11 0.5250734227173837 1103 12-13 -1.4151381011141666 1103 14-15 -0.7164504009768251 1103 16-17 0.08432053372348491 1103 18-19 -0.5879805285856463 1103 20-21 -0.7846397926069528 1103 22-23 0.010096759568206437 1103 24-25 0.6483743117070162 1103 26-27 -0.2638291369386607 1103 28-29 0.6225778251785954 1103 30-31 0.44874302333065685 1103 32-33 -5.487035720093054 1103 34-35 -0.8504559042494577 1103 36-37 -0.39713132048335353 1103 38-39 0.3679587761044587 1103 40-41 0.8752514070738187 1103 42-43 0.8437507639601165 1103 44-45 -4.586326194964386 1103 46-47 -0.19490582647206622 1103 48-49 0.3186676165943467 1103 50-51 0.787647399753979 1103 52-53 -0.06452341956459406 1103 54-55 0.6413721288011729 1103 56-57 1.2668702526002917 1103 58-59 1.25276042551031 1103 60-61 1.0969089328617514 1103 62-63 0.47003261311621003 1103 64-65 0.8331168449151889 1103 66-67 0.253232598374602 1103 68-69 -3.4203365661725087 1103 70-71 1.2775254626668158 1103 72-73 1.8259047360194245 1103 74-75 -0.5881840172326704 1103 76 0.09779639764626324 1104 1 -1.3455022679729147 1104 2 1.0317003218771212 1104 3 1.3411572710366073 1104 4 0.9630497742411066 1104 5 -0.28625423602834843 1104 6 0.6175846225237294 1104 7 0.7864875297973555 1104 8 2.9260242281178677 1104 9 0.40548110451447883 1104 10-11 1.0242325566253072 1104 12-13 -0.6355501254992788 1104 14-15 0.7485485479405583 1104 16-17 0.5390188730129495 1104 18-19 0.9626816534618641 1104 20-21 1.598795145379178 1104 22-23 0.5198718278885792 1104 24-25 1.1163162919466671 1104 26-27 0.6434653764100844 1104 28-29 0.37725514281576267 1104 30-31 -0.3771976956098513 1104 32-33 -1.4393165693678114 1104 34-35 0.44584428494540873 1104 36-37 0.9458369368216708 1104 38-39 -0.08400674838576805 1104 40-41 0.8070361453542461 1104 42-43 0.9354051679962794 1104 44-45 -2.389143096372834 1104 46-47 -0.39482173986285574 1104 48-49 1.4521551087112314 1104 50-51 1.2979480093818978 1104 52-53 1.0267105514255235 1104 54-55 0.14095169575513466 1104 56-57 0.24416686811427013 1104 58-59 0.8187684137381837 1104 60-61 0.5932301437089222 1104 62-63 1.1112981165847842 1104 64-65 1.0078067755437417 1104 66-67 0.6732452113659875 1104 68-69 -5.204444197032295 1104 70-71 -1.2471749787878892 1104 72-73 1.387182852479377 1104 74-75 -0.7267167059019997 1104 76 -0.7886866799236358 1105 1 -0.10669629782366385 1105 2 0.21080479948906117 1105 3 -0.3304845200081701 1105 4 -0.02202485262456122 1105 5 -0.032522892744765386 1105 6 0.438480144911793 1105 7 2.1894726044242176 1105 8 1.6722928848342846 1105 9 0.047272149290598975 1105 10-11 1.0167698700581411 1105 12-13 -1.3221172896783884 1105 14-15 0.3828769061495123 1105 16-17 1.1211084252517622 1105 18-19 0.8432786683872351 1105 20-21 0.725660817020966 1105 22-23 1.6840509323661976 1105 24-25 1.0267640531406954 1105 26-27 0.4792862719324731 1105 28-29 -0.1451329168857356 1105 30-31 0.913847080509548 1105 32-33 0.9487431321247257 1105 34-35 0.5801726431543628 1105 36-37 0.975687683090328 1105 38-39 0.1622619083306489 1105 40-41 0.12793166774230258 1105 42-43 0.3160021829216504 1105 44-45 1.1183195901943321 1105 46-47 0.9708499019281902 1105 48-49 0.10140884005451056 1105 50-51 -0.27667885628974176 1105 52-53 0.09387473052999695 1105 54-55 -0.06054084155829287 1105 56-57 0.1471519427411394 1105 58-59 0.48294751821579496 1105 60-61 0.13054357654473847 1105 62-63 1.1112981165847842 1105 64-65 0.007806775543741651 1105 66-67 0.5165287934555352 1105 68-69 2.2209289372960654 1105 70-71 1.1259593495703228 1105 72-73 0.5438992703898293 1105 74-75 -0.8311943178423036 1105 76 -0.9080896649982577 1106 1 0.10150000877891685 1106 2 0.7820165374096284 1106 3 -2.2024804724493556 1106 4 0.078911145351654 1106 5 0.557155832274205 1106 6 1.125553253942904 1106 7 0.9974665330859978 1106 8 1.9804132997090633 1106 9 2.081676399227355 1106 10-11 1.3442426755223522 1106 12-13 1.0034582225907727 1106 14-15 1.2236307639790098 1106 16-17 1.2265979268960763 1106 18-19 2.0507160577117958 1106 20-21 1.352147030023751 1106 22-23 0.19594063638844972 1106 24-25 1.0730580070997178 1106 26-27 0.9033945441308049 1106 28-29 0.1427497039085992 1106 30-31 0.3298602350757065 1106 32-33 1.489092234072622 1106 34-35 1.438634570804254 1106 36-37 0.8051842679625807 1106 38-39 1.2010931757225052 1106 40-41 1.151331617147818 1106 42-43 1.037353055676519 1106 44-45 1.8396704629492007 1106 46-47 1.1663975872304917 1106 48-49 1.2903792422806717 1106 50-51 1.6532477817067175 1106 52-53 1.1615448544864861 1106 54-55 1.1484143823223008 1106 56-57 2.0285078749445304 1106 58-59 1.070855436252728 1106 60-61 1.3386133969343206 1106 62-63 1.4258693283227046 1106 64-65 1.7672299984124464 1106 66-67 2.006156923989309 1106 68-69 2.154144216830595 1106 70-71 1.6171053146601295 1106 72-73 1.6448352683660517 1106 74-75 0.01613682306334141 1106 76 -0.24429001915965642 1107 1 -1.4229411604669657 1107 2 0.4292776526147364 1107 3 -1.0897317620587863 1107 4 0.40929679998196633 1107 5 -0.5462585622234606 1107 6 0.26262031363339844 1107 7 -2.1933956629346376 1107 8 -0.1254574937077848 1107 9 -0.9021113679060306 1107 10-11 -0.5985232296606497 1107 12-13 -0.19968596802578276 1107 14-15 -0.6646030851980953 1107 16-17 0.9572537854075023 1107 18-19 0.9583554583504608 1107 20-21 0.3435496338066031 1107 22-23 -0.713418243493642 1107 24-25 -0.23313211817662705 1107 26-27 0.16217617026688913 1107 28-29 -1.1704411582874243 1107 30-31 -0.6334166010824873 1107 32-33 1.4346829980126827 1107 34-35 0.2970231731132671 1107 36-37 0.555614137773432 1107 38-39 0.9021494272577542 1107 40-41 0.46169762058677577 1107 42-43 -0.32189528625421104 1107 44-45 -0.171751792025006 1107 46-47 0.15006253441791273 1107 48-49 -0.3773928038996317 1107 50-51 0.5897498696458001 1107 52-53 0.05223510258278452 1107 54-55 -0.0617305452139334 1107 56-57 0.45874614563968663 1107 58-59 1.0384309705194923 1107 60-61 -0.5171959865095488 1107 62-63 -0.9903674685331083 1107 64-65 -0.22699746412747146 1107 66-67 1.2687459684501263 1107 68-69 1.0033213231926652 1107 70-71 0.3778520599315556 1107 72-73 -0.35086603352537793 1107 74-75 0.31557185131192256 1107 76 -0.1369453863912966 1108 1 0.5635011022726317 1108 2 0.07069887748617276 1108 3 -0.04064340301250269 1108 4 0.11663667842984182 1108 5 0.5823061876596611 1108 6 0.03260628838796009 1108 7 1.5127754450597521 1108 8 1.2628080509392419 1108 9 1.6322504834263682 1108 10-11 1.0956095426628707 1108 12-13 0.8291826621752421 1108 14-15 0.7286881723989111 1108 16-17 -0.08832826227833124 1108 18-19 0.5069763092153536 1108 20-21 0.5266959638673825 1108 22-23 -0.6737969251205627 1108 24-25 0.30228162656390367 1108 26-27 -0.2593993323044046 1108 28-29 0.9252811418528282 1108 30-31 1.065508130100305 1108 32-33 0.8265717310655063 1108 34-35 -1.349774395844186 1108 36-37 0.21702133739943008 1108 38-39 1.381929698412499 1108 40-41 0.36866349248953867 1108 42-43 0.34886207699964444 1108 44-45 0.5221472262078066 1108 46-47 0.9601373357269338 1108 48-49 0.5988811558946665 1108 50-51 0.9860799304218659 1108 52-53 0.39117853409283043 1108 54-55 0.8580918016771406 1108 56-57 1.2761842008056519 1108 58-59 -1.5141636998872414 1108 60-61 0.25933510278451166 1108 62-63 0.2731902687272978 1108 64-65 0.07641534559670049 1108 66-67 -1.2093980240697348 1108 68-69 1.0521759281482588 1108 70-71 2.6791610828394568 1108 72-73 1.4890124143474566 1108 74-75 0.240303034107626 1108 76 0.5996137533936547 1109 1 -0.6203186621922967 1109 2 0.03383251803916565 1109 3 0.6500888038487389 1109 4 -0.7128564091277632 1109 5 0.4578822245260241 1109 6 1.1887762832676287 1109 7 -0.7655143654881229 1109 8 0.26101593624390773 1109 9 0.6665566790228894 1109 10-11 0.8248722154739241 1109 12-13 0.19541410098732825 1109 14-15 0.5106902205299875 1109 16-17 -1.0596544271528785 1109 18-19 0.22612633481699618 1109 20-21 0.9674281707286241 1109 22-23 0.983269127106773 1109 24-25 0.3341556665024612 1109 26-27 -0.24672652124449712 1109 28-29 -0.5896958166725099 1109 30-31 -0.3715118391777281 1109 32-33 -0.21093466934411964 1109 34-35 -0.01388089866036779 1109 36-37 -0.22319883669170792 1109 38-39 0.5574289303633435 1109 40-41 0.3888887869083817 1109 42-43 -9.84313169325901E-4 1109 44-45 -1.1589843662140993 1109 46-47 0.12590794504593106 1109 48-49 -0.08788279699832202 1109 50-51 0.10320742658161919 1109 52-53 -1.2038035447602198 1109 54-55 0.505557239465162 1109 56-57 0.57572337131257 1109 58-59 0.1550631306299124 1109 60-61 0.700195317838272 1109 62-63 -0.2691804429982554 1109 64-65 -0.6207172708907578 1109 66-67 0.9481800609277045 1109 68-69 1.6102916475542912 1109 70-71 0.026575317350470584 1109 72-73 -1.0278846670657487 1109 74-75 1.188587724327796 1109 76 -0.0893263387722456 1110 1 0.8130446202275898 1110 2 1.5217031379852592 1110 3 -0.6565898424525507 1110 4 0.7831004641876049 1110 5 0.177558774391251 1110 6 1.1354669092035365 1110 7 1.6786305880907904 1110 8 2.9234897307931718 1110 9 -2.2630629675778913 1110 10-11 -0.07404961741016791 1110 12-13 0.12817840167615913 1110 14-15 0.3816096574871679 1110 16-17 0.5105761808135725 1110 18-19 0.737956224005373 1110 20-21 -0.25434481519531005 1110 22-23 0.8534398369001295 1110 24-25 1.3669499162778393 1110 26-27 -0.13082355628493048 1110 28-29 1.3873931321145463 1110 30-31 0.6087217636973818 1110 32-33 1.463668505259058 1110 34-35 0.6134027191892883 1110 36-37 0.2661692375820195 1110 38-39 0.9761171603723255 1110 40-41 0.8504042106879552 1110 42-43 1.2141998737129711 1110 44-45 1.77123426211773 1110 46-47 1.0357611945218252 1110 48-49 1.625204953825282 1110 50-51 0.4500178488637374 1110 52-53 -0.9889188487434453 1110 54-55 -0.6251705233380704 1110 56-57 0.05281232009962622 1110 58-59 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56-57 -0.9410833513765127 2116 58-59 -1.0146380831889417 2116 60-61 -1.0252948782401603 2116 62-63 0.3538354300176181 2116 64-65 0.24529580100467285 2116 66-67 -0.7231551398192622 2116 68-69 -0.9041808080946296 2116 70-71 -4.389624495908169 2116 72-73 -0.5166801852730316 2116 74-75 -3.54717324672729 2116 76 -2.333023817763845 2117 1 0.14414626356295202 2117 2 0.6190859742603649 2117 3 0.7335501453745934 2117 4 1.0843786139137137 2117 5 0.06617715540159708 2117 6 1.0971224174202163 2117 7 -1.503353587198312 2117 8 -1.7210629168026905 2117 9 1.1483795156844323 2117 10-11 0.3778676071789988 2117 12-13 2.1598278234655695 2117 14-15 0.7206236562698791 2117 16-17 1.0890910925603805 2117 18-19 1.2489117930863216 2117 20-21 1.14766370580287 2117 22-23 1.7052353329439498 2117 24-25 0.5038945297897044 2117 26-27 1.2647942160826915 2117 28-29 0.17528114185283528 2117 30-31 0.8397016784874083 2117 32-33 1.6410878600977625 2117 34-35 0.10183850738161482 2117 36-37 -0.7587851142134738 2117 38-39 0.6319296984124989 2117 40-41 -0.5990784429943332 2117 42-43 -0.9656540520326118 2117 44-45 1.1753730326594223 2117 46-47 0.21013733572693383 2117 48-49 0.6714618010559619 2117 50-51 1.3489831562283143 2117 52-53 2.2298882115121827 2117 54-55 0.6161563178061726 2117 56-57 -0.046396444355629285 2117 58-59 0.14712662269340626 2117 60-61 0.4367544576232234 2117 62-63 1.055448333243426 2117 64-65 0.6731895391450848 2117 66-67 -0.4432689918116708 2117 68-69 0.3424985087934118 2117 70-71 1.6307739860652646 2117 72-73 1.359980156282944 2117 74-75 0.41772238894633773 2117 76 0.29316214049042344 2118 1 0.8538236829177919 2118 2 0.0061827484539129784 2118 3 0.7658082098907215 2118 4 1.0843786139137137 2118 5 1.0016610263693408 2118 6 2.1938966109686007 2118 7 1.3030980257049123 2118 8 -3.0113854974478507 2118 9 -2.367749516573628 2118 10-11 -1.3318098121758482 2118 12-13 1.4501504041107296 2118 14-15 -0.6180860211494732 2118 16-17 -0.007683100988010949 2118 18-19 0.2972988898605138 2118 20-21 -2.4007233909713293 2118 22-23 -0.3270227315721783 2118 24-25 0.24583001366067947 2118 26-27 1.5389877644697876 2118 28-29 1.2881843676592837 2118 30-31 0.19454038816481756 2118 32-33 0.7056039891300259 2118 34-35 1.0050643138332234 2118 36-37 -0.08136575937476209 2118 38-39 0.664187762928627 2118 40-41 -1.8894010236394934 2118 42-43 0.29241046409642024 2118 44-45 0.9173085165303974 2118 46-47 0.46820185185596586 2118 48-49 -0.8285381989440381 2118 50-51 -1.0865007147394294 2118 52-53 0.2137591792541258 2118 54-55 0.30970470490294133 2118 56-57 1.0665067814508191 2118 58-59 0.08261049366115003 2118 60-61 -1.2084068326993602 2118 62-63 -0.49293876353076627 2118 64-65 -0.24616529956459487 2118 66-67 -2.28197866923103 2118 68-69 1.5683049604063228 2118 70-71 -0.4498711752250557 2118 72-73 -0.9142133921041591 2118 74-75 0.5144965824947221 2118 76 1.422194398554943 2119 1 0.2421152002415674 2119 2 -0.4741039898973405 2119 3 -0.2652551114951791 2119 4 -0.6599463562177093 2119 5 1.1510039176477136 2119 6 1.8713159658073124 2119 7 0.5731099731362193 2119 8 0.5202751955031673 2119 9 0.05280006526627545 2119 10-11 -0.7941754035736963 2119 12-13 0.8276892332624612 2119 14-15 0.9762986861384562 2119 16-17 -0.9670618345602904 2119 18-19 -2.8717572630666055 2119 20-21 -1.6331009298004773 2119 22-23 -0.07493193109428375 2119 24-25 -1.097061264714469 2119 26-27 -1.720271494789472 2119 28-29 0.16691793994124282 2119 30-31 -0.28156474923063257 2119 32-33 -0.7400351984446445 2119 34-35 0.3300344452549737 2119 36-37 -0.590923704416582 2119 38-39 -0.11717424423983402 2119 40-41 0.30952370754330616 2119 42-43 -0.8396086517936681 2119 44-45 -1.0372912445209863 2119 46-47 -0.11483279569480942 2119 48-49 -0.24132195043747373 2119 50-51 1.8095566329308213 2119 52-53 -0.1297294706861436 2119 54-55 -0.48121524730733256 2119 56-57 -0.5565517609625701 2119 58-59 0.8137932893600777 2119 60-61 -1.0405454229024684 2119 62-63 -1.6183867922046034 2119 64-65 0.3422456920722112 2119 66-67 -0.022719409971770688 2119 68-69 -0.11986708260442924 2119 70-71 -0.4522606614855107 2119 72-73 -0.742170381351464 2119 74-75 0.8155718513119297 2119 76 2.566758317312413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 7.0 15 24.0 16 57.0 17 82.0 18 95.0 19 96.0 20 70.0 21 84.0 22 111.0 23 100.0 24 132.0 25 206.0 26 212.0 27 289.0 28 317.0 29 415.0 30 518.0 31 662.0 32 871.0 33 1209.0 34 1777.0 35 2762.0 36 4671.0 37 4303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.218014977323065 19.175192490243646 10.515768378862989 27.0910241535703 2 18.254259501965926 18.89908256880734 40.71821756225426 22.128440366972477 3 18.427260812581913 24.262123197903016 30.631716906946266 26.678899082568808 4 14.589777195281783 18.422018348623855 39.31847968545216 27.6697247706422 5 13.892529488859765 37.085190039318476 36.471821756225424 12.55045871559633 6 28.854521625163827 37.876802096985585 17.49934469200524 15.769331585845347 7 27.22935779816514 29.546526867627787 23.150720838794232 20.073394495412845 8 24.267365661861074 34.07601572739188 20.87024901703801 20.786369593709043 9 24.938401048492793 15.732634338138926 22.883355176933158 36.44560943643512 10-11 25.769331585845347 25.60419397116645 27.520314547837483 21.106159895150718 12-13 24.623853211009177 23.98951507208388 26.69724770642202 24.68938401048493 14-15 22.228047182175622 25.85058977719528 24.60288335517693 27.318479685452164 16-17 22.37745740498034 27.918741808650065 27.25294888597641 22.450851900393186 18-19 22.477064220183486 27.29488859764089 27.313237221494102 22.91480996068152 20-21 21.903014416775886 27.494102228047186 27.190039318479688 23.412844036697248 22-23 22.92529488859764 26.380078636959368 27.850589777195285 22.84403669724771 24-25 22.102228047182177 27.6697247706422 28.020969855832238 22.20707732634338 26-27 21.242463958060288 28.408912188728703 28.676277850589777 21.672346002621232 28-29 22.102228047182177 27.258191349934467 29.255570117955436 21.384010484927916 30-31 22.671035386631715 27.15858453473132 28.220183486238533 21.950196592398427 32-33 21.200524246395805 28.503276539973786 27.82961992136304 22.466579292267365 34-35 22.642201834862384 27.926605504587155 28.188728702490167 21.242463958060288 36-37 22.90956749672346 27.6173001310616 27.63826998689384 21.834862385321102 38-39 22.013106159895152 27.879423328964613 28.47444298820446 21.63302752293578 40-41 22.424639580602886 26.946264744429882 28.445609436435127 22.18348623853211 42-43 22.031454783748362 27.465268676277848 28.078636959370908 22.424639580602886 44-45 21.78768020969856 27.436435124508517 27.82437745740498 22.95150720838794 46-47 21.968545216251638 27.313237221494102 28.435124508519007 22.283093053735257 48-49 21.11926605504587 27.289646133682833 29.106159895150718 22.484927916120576 50-51 21.520314547837483 26.988204456094365 28.52948885976409 22.96199213630406 52-53 21.4521625163827 26.744429882044564 28.917431192660555 22.88597640891219 54-55 20.37221494102228 27.363040629095675 29.520314547837483 22.74442988204456 56-57 20.36697247706422 26.673656618610746 29.66710353866317 23.29226736566186 58-59 21.404980340760158 26.83355176933159 28.980340760157276 22.781127129750985 60-61 20.55045871559633 26.66579292267366 29.580602883355176 23.203145478374836 62-63 20.144167758846656 27.231979030144167 29.231979030144167 23.391874180865006 64-65 21.148099606815205 27.386631716906944 28.401048492791613 23.06422018348624 66-67 20.096985583224114 27.07470511140236 28.917431192660555 23.910878112712975 68-69 19.8086500655308 27.528178243774576 28.972477064220183 23.690694626474443 70-71 21.069462647444297 27.32634338138925 28.335517693315857 23.268676277850588 72-73 21.192660550458715 26.309305373525554 27.672346002621236 24.825688073394495 74-75 20.78899082568807 26.38532110091743 28.621231979030142 24.204456094364353 76 21.719528178243774 25.95543905635649 27.769331585845347 24.55570117955439 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.5 17 2.0 18 1.5 19 1.5 20 8.0 21 14.0 22 13.5 23 20.0 24 29.5 25 32.0 26 45.0 27 70.5 28 117.0 29 151.0 30 161.0 31 219.0 32 303.5 33 340.0 34 405.5 35 512.0 36 613.5 37 674.0 38 758.0 39 937.0 40 1112.5 41 1193.0 42 1193.0 43 1279.5 44 1411.0 45 1410.5 46 1365.0 47 1341.0 48 1264.5 49 1201.5 50 1191.0 51 1077.0 52 853.5 53 674.5 54 605.0 55 569.5 56 460.0 57 340.5 58 295.0 59 239.0 60 151.5 61 105.0 62 90.0 63 68.5 64 35.0 65 19.5 66 12.5 67 9.0 68 7.0 69 5.5 70 5.0 71 4.0 72 5.0 73 5.0 74 3.0 75 2.0 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5923984272608126 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 19075.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.43643512450852 #Duplication Level Percentage of deduplicated Percentage of total 1 80.31838949171902 58.98296199213631 2 11.914620217018847 17.49934469200524 3 3.997715591090805 8.807339449541285 4 1.7061679040548259 5.011795543905636 5 0.9066247858366648 3.328964613368283 6 0.49257567104511707 2.1703800786369594 7 0.1927470017133067 0.9908256880733944 8 0.20702455739577386 1.2162516382699868 9 0.07852655625356939 0.5190039318479686 >10 0.18560822387207312 1.473132372214941 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.005242463958060288 0.0 26 0.0 0.0 0.0 0.005242463958060288 0.0 27 0.0 0.0 0.0 0.020969855832241154 0.0 28 0.0 0.0 0.0 0.020969855832241154 0.0 29 0.0 0.0 0.0 0.020969855832241154 0.0 30 0.0 0.0 0.0 0.020969855832241154 0.0 31 0.0 0.0 0.0 0.02621231979030144 0.0 32 0.0 0.0 0.0 0.03669724770642202 0.0 33 0.0 0.0 0.0 0.05242463958060288 0.0 34 0.0 0.0 0.0 0.06815203145478375 0.0 35 0.0 0.0 0.0 0.07339449541284404 0.0 36 0.0 0.0 0.0 0.09436435124508519 0.0 37 0.0 0.0 0.0 0.1258191349934469 0.0 38 0.0 0.0 0.0 0.22018348623853212 0.0 39 0.0 0.0 0.0 0.3984272608125819 0.0 40 0.0 0.0 0.0 0.581913499344692 0.0 41 0.0 0.0 0.0 0.7182175622542595 0.0 42 0.0 0.0 0.0 0.9174311926605505 0.0 43 0.0 0.0 0.0 1.2791612057667103 0.0 44 0.0 0.0 0.0 1.5727391874180865 0.0 45 0.0 0.0 0.0 1.908256880733945 0.0 46 0.0 0.0 0.0 2.2961992136304064 0.0 47 0.0 0.0 0.0 2.6264744429882043 0.0 48 0.0 0.0 0.0 2.977719528178244 0.0 49 0.0 0.0 0.0 3.323722149410223 0.0 50 0.0 0.0 0.0 3.769331585845347 0.0 51 0.0 0.0 0.0 4.18872870249017 0.0 52 0.0 0.0 0.0 4.697247706422019 0.0 53 0.0 0.0 0.0 5.237221494102228 0.0 54 0.0 0.0 0.0 5.834862385321101 0.0 55 0.0 0.0 0.0 6.296199213630406 0.0 56 0.0 0.0 0.0 6.783748361730013 0.0 57 0.0 0.0 0.0 7.323722149410223 0.0 58 0.0 0.0 0.0 7.785058977719528 0.0 59 0.0 0.0 0.0 8.325032765399738 0.0 60 0.0 0.0 0.0 8.812581913499345 0.0 61 0.0 0.0 0.0 9.357798165137615 0.0 62 0.0 0.0 0.0 9.771952817824378 0.0 63 0.0 0.0 0.0 10.285714285714286 0.0 64 0.0 0.0 0.0 10.830930537352556 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGAG 15 0.0021923555 69.99213 46 TCGGCTT 15 0.0021923555 69.99213 24 CAATCAA 15 0.0021923555 69.99213 9 AAGACGC 15 0.0021923555 69.99213 15 GACGCCC 15 0.0021923555 69.99213 17 AGACGCC 15 0.0021923555 69.99213 16 CAAAGGT 15 0.0021923555 69.99213 61 CGGCTTA 15 0.0021923555 69.99213 25 TCTCTTC 15 0.0021923555 69.99213 34 CCCTCGG 15 0.0021923555 69.99213 21 CTTCTCT 15 0.0021923555 69.99213 32 AATGACA 15 0.0021923555 69.99213 52 ACTACAC 20 7.734811E-5 69.99213 3 TTACTAC 20 0.0066394256 52.91071 1 GCCCTCG 20 0.006851123 52.494095 20 TAAATTA 20 0.006851123 52.494095 22 ACGCCCT 20 0.006851123 52.494095 18 TTACTTC 20 0.006851123 52.494095 29 CGCCCTC 20 0.006851123 52.494095 19 ATGACAT 20 0.006851123 52.494095 53 >>END_MODULE