FastQCFastQC Report
Wed 25 May 2016
SRR1780530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19075
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTG150.002191377870.046
ATGGGCG150.002191377870.011
TTGTTGG150.002191377870.050
GTATGAC150.002191377870.03
ATGACTA150.002191377870.05
TTACTGG150.002191377870.032
GGCGTTG207.7305005E-570.014
GTGTATA150.002191377870.056
GAATGTT150.002191377870.055
TATGACT150.002191377870.04
ACATTGT150.002191377870.043
TTGATTA150.002191377870.018
GCGTTGA150.002191377870.015
TGGGCGT150.002191377870.012
CTATGGG150.002191377870.09
CGTTGAT150.002191377870.016
TGTATAT150.002191377870.057
GTAGTAG150.002191377870.025
AACATTG150.002191377870.042
GGTGTAT150.002191377870.055
Produced by FastQC (version 0.11.5)