FastQCFastQC Report
Wed 25 May 2016
SRR1780529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287363
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCT207.8305404E-444.01830338
CCTTAGG207.87772E-443.9646571
CATTGAT207.87772E-443.96465727
CGCAAAG250.002335010335.24535812
GCCCAAT250.00234502335.2146437
GGATCCG353.181104E-431.48005321
CCGAAGT353.181104E-431.48005314
CGTCCAA353.2138126E-431.4252122
AATTATC701.035005E-931.40879430
GGGTAAG353.2269736E-431.4033261
CCATAGC502.3776447E-630.7591979
CCGCCTA602.8859904E-729.38651315
ATTAACG300.005692080629.38138618
CCCAACG300.005692080629.38138621
GGGCACT300.00571153229.3608911
GGCTCGA300.00571153229.3608911
TGCTTAT300.005726155429.34553740
CCGACTC300.005726155429.34553737
CCAACGT300.00574080829.33019822
TTAGATC300.00576039129.30977241
Produced by FastQC (version 0.11.5)