FastQCFastQC Report
Wed 25 May 2016
SRR1780529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287363
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT3470.12075319369577853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG250.002350366235.1983323
CGTTCGG250.002350366235.1983324
TTCGGTA250.002350366235.1983326
CGGTAAG250.002350366235.1983328
CATCGAC353.2127023E-431.427087
GACTATC353.2127023E-431.4270838
ATCGACC353.2127023E-431.427088
ACGGGTG300.005739152429.3319427
TCGCTAT300.005739152429.33194211
CTCGAAT300.005739152429.33194233
GCGTCAA300.005739152429.33194226
CGTCAAA300.005739152429.33194227
TCGGTAA300.005739152429.33194227
GGAGTTT701.127717E-625.14166533
GTACGGA450.001394595324.4475386
AAAGCGA450.001396010424.44328525
CCATAGA551.5881346E-424.0030382
AGTTGTT852.3407847E-723.2930120
GCCATAG602.8427847E-422.0257841
CTATGGC602.8629514E-422.0027873
Produced by FastQC (version 0.11.5)