FastQCFastQC Report
Wed 25 May 2016
SRR1780528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32472
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACT150.002205996369.9953815
CTTACGC150.002205996369.9953812
AGATCTC200.00689341852.49653237
GCTGAGC200.00689341852.4965328
CCACTGC200.00689341852.49653248
TAGATCT200.00689341852.49653236
AGTACGT200.00689341852.4965325
GACGAGC900.005171294319.44316169
CCGACGA1450.00461163414.48180267
GCCGACG1500.005775321313.99907666
Produced by FastQC (version 0.11.5)