##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780528_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32472 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.368656072924367 34.0 27.0 34.0 12.0 34.0 2 30.44542990884454 34.0 32.0 34.0 21.0 34.0 3 30.62318305001232 34.0 32.0 34.0 21.0 34.0 4 30.895941118502094 34.0 32.0 34.0 21.0 34.0 5 30.90410199556541 34.0 33.0 34.0 21.0 34.0 6 33.47348484848485 38.0 34.0 38.0 21.0 38.0 7 32.623275437299824 37.0 31.0 38.0 11.0 38.0 8 32.15579576250308 37.0 31.0 38.0 11.0 38.0 9 32.77685390490269 37.0 32.0 38.0 12.0 38.0 10-11 33.09425043114068 37.0 34.0 38.0 16.0 38.0 12-13 33.281365484109386 37.5 34.0 38.0 16.0 38.0 14-15 33.089045947277654 37.0 34.0 38.0 11.5 38.0 16-17 32.89832779009608 37.0 34.0 38.0 11.5 38.0 18-19 32.90072985957132 37.0 33.0 38.0 11.0 38.0 20-21 33.03569228874107 37.0 34.0 38.0 16.0 38.0 22-23 32.92407304754866 37.0 33.5 38.0 11.0 38.0 24-25 33.28977272727273 37.5 34.0 38.0 16.0 38.0 26-27 33.28039541759054 38.0 34.0 38.0 15.5 38.0 28-29 33.3989591032274 38.0 34.0 38.0 11.0 38.0 30-31 33.36114190687361 38.0 34.0 38.0 11.0 38.0 32-33 32.989406257698946 37.0 34.0 38.0 11.0 38.0 34-35 33.099116161616166 37.0 34.0 38.0 11.0 38.0 36-37 33.11559189455531 37.0 34.0 38.0 11.0 38.0 38-39 33.41346390736635 38.0 34.0 38.0 11.0 38.0 40-41 32.99785969450603 37.5 34.0 38.0 11.0 38.0 42-43 33.31909029317566 37.5 34.0 38.0 16.0 38.0 44-45 32.430647942843066 37.0 31.5 38.0 11.0 38.0 46-47 33.13078960335058 37.0 34.0 38.0 15.5 38.0 48-49 32.93198755851195 37.0 33.5 38.0 11.0 38.0 50-51 32.75951589061346 37.0 32.5 38.0 11.0 38.0 52-53 32.79982754372999 37.0 32.5 38.0 11.0 38.0 54-55 32.62824895294408 37.0 32.0 38.0 11.0 38.0 56-57 32.61057218526731 37.0 32.0 38.0 11.0 38.0 58-59 32.54092756836659 37.0 31.5 38.0 11.0 38.0 60-61 32.58080808080808 37.0 31.5 38.0 11.0 38.0 62-63 32.52777777777778 37.0 31.5 38.0 11.0 38.0 64-65 32.654933481152995 37.0 32.0 38.0 11.0 38.0 66-67 32.66571199802907 37.0 31.5 38.0 11.0 38.0 68-69 32.04705592510471 37.0 29.5 38.0 11.0 38.0 70-71 32.112635501355015 37.0 31.0 38.0 11.0 38.0 72-73 32.214215323971416 37.0 31.0 38.0 11.0 38.0 74-75 31.733231707317074 37.0 29.0 38.0 11.0 38.0 76 30.89908228627741 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.857827233298881 1101 2 -2.036224333078856 1101 3 -0.8492310478599947 1101 4 -1.8752212067765655 1101 5 -2.1832856617632004 1101 6 -2.230899720716021 1101 7 -1.6614372524293444 1101 8 -2.267080347251948 1101 9 -1.1232058906927982 1101 10-11 -2.1077919418341224 1101 12-13 -2.438410824509596 1101 14-15 -1.6376359415355317 1101 16-17 -2.832741690486632 1101 18-19 -1.7481491868484405 1101 20-21 -2.078202250135 1101 22-23 -1.5657297598974154 1101 24-25 -2.4016657418913994 1101 26-27 -1.7665515078606298 1101 28-29 -1.5652312480998916 1101 30-31 -2.971754263534301 1101 32-33 -1.9468432093212442 1101 34-35 -2.6626552237954044 1101 36-37 -1.7492413163599565 1101 38-39 -1.4596802559831374 1101 40-41 -0.9309459623585603 1101 42-43 -1.5471483351202124 1101 44-45 -1.4553698068344758 1101 46-47 -0.8732256325211836 1101 48-49 -1.2217686793007445 1101 50-51 -2.6321581803513965 1101 52-53 -1.2208614304720626 1101 54-55 -2.5506090368893055 1101 56-57 -2.939818679958009 1101 58-59 -1.527619015278706 1101 60-61 -0.8931660587519445 1101 62-63 -2.5584332562825765 1101 64-65 -2.027064541147336 1101 66-67 -1.6183667865707037 1101 68-69 -0.8510946820568961 1101 70-71 -0.8711006064315825 1101 72-73 -0.8719237827850996 1101 74-75 -2.4330392797598073 1101 76 -3.1898377005563994 1102 1 0.8534822905106445 1102 2 -0.7832481426026661 1102 3 0.19541180928286295 1102 4 1.2438264122710514 1102 5 1.2408214810939455 1102 6 1.2497550411887417 1102 7 1.9367770332849403 1102 8 2.1659553670337672 1102 9 0.8440560140691034 1102 10-11 -0.20600622754841424 1102 12-13 -3.7553751102238806 1102 14-15 -0.6071299891545792 1102 16-17 -2.2241107381056793 1102 18-19 -0.7585658535151083 1102 20-21 1.1740298927221389 1102 22-23 0.46254404962638773 1102 24-25 1.5871735438228853 1102 26-27 -4.017295555479677 1102 28-29 1.1051556566620135 1102 30-31 0.7767576412276043 1102 32-33 -6.830027733130766 1102 34-35 -0.004917128557309525 1102 36-37 1.1257586836400435 1102 38-39 1.3587721249692422 1102 40-41 0.9991135614509687 1102 42-43 1.0190719029750213 1102 44-45 -4.622780521120191 1102 46-47 0.43481008176453173 1102 48-49 0.7960884635563943 1102 50-51 -5.8977831803513965 1102 52-53 -0.7878257161863473 1102 54-55 0.9017719154916506 1102 56-57 -0.5581222513865782 1102 58-59 1.6368155085308231 1102 60-61 1.1730541793432891 1102 62-63 -3.482540399139719 1102 64-65 0.6544830779002808 1102 66-67 1.2730022610483473 1102 68-69 -5.929219682056896 1102 70-71 0.667589869758892 1102 72-73 1.2858143124529988 1102 74-75 -5.235122613093143 1102 76 -1.359480557699257 1103 1 1.0028049506091676 1103 2 0.6527124485303375 1103 3 0.9632689521400053 1103 4 0.9193190231084891 1103 5 0.20572295892645442 1103 6 -0.3986439735895857 1103 7 0.09502826481203641 1103 8 1.4969282734377103 1103 9 1.2532308909163987 1103 10-11 2.0430701271313936 1103 12-13 0.029261589283507305 1103 14-15 0.8409919320276913 1103 16-17 0.3123732520421001 1103 18-19 0.6024255257952404 1103 20-21 0.31673309469258726 1103 22-23 0.12679897573476495 1103 24-25 0.502967878798259 1103 26-27 -0.04869949636637472 1103 28-29 0.8613132921299993 1103 30-31 0.5275273456610989 1103 32-33 -2.95456591047067 1103 34-35 -0.9862902812666725 1103 36-37 0.5093793732952179 1103 38-39 1.0841415830973204 1103 40-41 1.2001603594805204 1103 42-43 1.1663933315464519 1103 44-45 -4.814744806834476 1103 46-47 0.34229160885812604 1103 48-49 0.6898692517337395 1103 50-51 0.39963061275204836 1103 52-53 0.1756902936658662 1103 54-55 0.521230043570462 1103 56-57 0.39153907866268156 1103 58-59 0.5673881686293427 1103 60-61 0.6414962975698941 1103 62-63 0.6333770885450107 1103 64-65 0.9258808611515121 1103 66-67 -0.5867575911684 1103 68-69 -2.4637024406775865 1103 70-71 0.6292585889707212 1103 72-73 0.9822428838815682 1103 74-75 -1.9387864061965914 1103 76 -0.23725149365984777 1104 1 -0.9022261404027034 1104 2 0.4210844100905433 1104 3 0.315727968533448 1104 4 0.3372104872125057 1104 5 -0.10490451422221625 1104 6 -0.20955409230072064 1104 7 1.3744234033083629 1104 8 1.510071292092313 1104 9 0.5257012131323364 1104 10-11 -0.29016899101445404 1104 12-13 -0.34927148024730315 1104 14-15 -0.13413525847542473 1104 16-17 0.26220366470462153 1104 18-19 0.9683808677963697 1104 20-21 1.1075047170781147 1104 22-23 0.08864182480203908 1104 24-25 0.4136518810594154 1104 26-27 0.8711704866748917 1104 28-29 -0.10356799673376571 1104 30-31 -1.3341176515124396 1104 32-33 -0.31868405631578156 1104 34-35 0.15821226254338683 1104 36-37 0.6278078639679165 1104 38-39 -0.4784644089886072 1104 40-41 0.40733955676712696 1104 42-43 0.4553277577759616 1104 44-45 -3.2307284133918515 1104 46-47 0.2025940396099628 1104 48-49 0.6952395174205677 1104 50-51 0.6472987868617182 1104 52-53 0.561925454773835 1104 54-55 -0.7138604030095266 1104 56-57 0.2853350085665767 1104 58-59 1.0312095366338596 1104 60-61 -0.12327193306888518 1104 62-63 0.36062412076660877 1104 64-65 0.28526469382534003 1104 66-67 -0.19896787946687766 1104 68-69 -3.546022960745418 1104 70-71 -1.0710664534261127 1104 72-73 -0.2821013784135147 1104 74-75 -1.3401431048964234 1104 76 -0.42959179891705546 1105 1 -1.9889247942744888 1105 2 0.8616415205796812 1105 3 -0.7155928364778816 1105 4 0.11563245176001757 1105 5 1.0684927935213508 1105 6 1.4136632874140602 1105 7 1.3736237231804083 1105 8 0.7072590836423629 1105 9 -0.39353109394483 1105 10-11 0.7163950500357998 1105 12-13 -1.218390499306345 1105 14-15 -0.10041541308024193 1105 16-17 0.5097379843101137 1105 18-19 0.6492085367287928 1105 20-21 -0.2505853802163003 1105 22-23 -0.08198992249904791 1105 24-25 0.3799653556695759 1105 26-27 -0.0926439875354248 1105 28-29 -0.8331428334657431 1105 30-31 0.09277825679090768 1105 32-33 1.3551130914917664 1105 34-35 0.7303833940907722 1105 36-37 0.7731164072172803 1105 38-39 0.9249742155615763 1105 40-41 0.6628040376414361 1105 42-43 1.258873006343201 1105 44-45 1.8732633232468245 1105 46-47 1.1684410341454878 1105 48-49 1.0165443288293332 1105 50-51 0.32985909607136676 1105 52-53 -0.43630858494361036 1105 54-55 -0.8422757035559734 1105 56-57 0.1609181086598781 1105 58-59 -0.3958862917014656 1105 60-61 0.735517884337483 1105 62-63 1.5731724347743352 1105 64-65 0.521970011698194 1105 66-67 1.0056169532667028 1105 68-69 1.6652925130650544 1105 70-71 1.7023241903163893 1105 72-73 0.9873241846945788 1105 74-75 -1.4492994423614363 1105 76 -0.6766263184425796 1106 1 -0.0705853453932761 1106 2 0.08748510644916507 1106 3 0.04733974860018364 1106 4 0.42824486991959887 1106 5 -0.33455778565700456 1106 6 0.4992809577500523 1106 7 1.7627884112874668 1106 8 1.5252315996507093 1106 9 2.4335787700741633 1106 10-11 -0.2836768975863322 1106 12-13 0.01900067991518739 1106 14-15 0.2213703269010523 1106 16-17 0.7620223213127773 1106 18-19 0.059741668608786824 1106 20-21 -0.933152471373937 1106 22-23 -0.20879760650508672 1106 24-25 -0.07934826401529449 1106 26-27 -0.32402754030901093 1106 28-29 -0.6067596404302762 1106 30-31 -0.5206111956876924 1106 32-33 0.2220819381713781 1106 34-35 0.47331454021639274 1106 36-37 0.46978523231260993 1106 38-39 -0.1740976601129347 1106 40-41 0.43566539457359 1106 42-43 0.5259066058827315 1106 44-45 -0.04593949709995826 1106 46-47 0.9796504736735088 1106 48-49 -0.6382509801856955 1106 50-51 0.5668185895601141 1106 52-53 0.1375456491739513 1106 54-55 -0.3142521047359139 1106 56-57 0.424534196148187 1106 58-59 -0.4706038972846045 1106 60-61 -0.19566421509413345 1106 62-63 0.1014118764607872 1106 64-65 0.47081525236298916 1106 66-67 0.12425858216086283 1106 68-69 0.36613430024398497 1106 70-71 0.6223544083176833 1106 72-73 -0.4303677355874598 1106 74-75 -0.6247796927391605 1106 76 -0.8236872580785217 1107 1 -0.6096388275017794 1107 2 -0.7469127388759595 1107 3 -1.058470178294776 1107 4 -0.8125400473562756 1107 5 -0.42051392263276455 1107 6 1.0200966560955749 1107 7 -0.010893774168472703 1107 8 1.3989522614437035 1107 9 -0.9920464704029435 1107 10-11 0.2867732755571808 1107 12-13 -0.6030847375530684 1107 14-15 -0.21702905747756063 1107 16-17 -0.017161980341704464 1107 18-19 -0.037641940471630164 1107 20-21 0.34068361943021586 1107 22-23 -0.5425413541003152 1107 24-25 -0.025442915804440247 1107 26-27 0.5219901588060409 1107 28-29 -0.3717076973752498 1107 30-31 0.14799211327729722 1107 32-33 0.5931477327077417 1107 34-35 0.5309136167843036 1107 36-37 -0.051415229403431795 1107 38-39 0.5662255411183139 1107 40-41 0.7512098347428875 1107 42-43 -0.17382405975789794 1107 44-45 0.4906899757742238 1107 46-47 0.4191656718266472 1107 48-49 0.011383494612296374 1107 50-51 0.9891733413877404 1107 52-53 1.431312482571414 1107 54-55 0.6867098036904054 1107 56-57 1.1603171896072126 1107 58-59 -0.27983821817725385 1107 60-61 -0.5399504790418028 1107 62-63 0.21276239589133894 1107 64-65 0.1782796617512119 1107 66-67 1.1780100250235037 1107 68-69 0.8718672744648472 1107 70-71 0.19946098777132448 1107 72-73 0.6311559273598277 1107 74-75 0.1510186912546807 1107 76 0.19385795161751318 1108 1 -0.10926401490807436 1108 2 0.4199538278406827 1108 3 0.5149930900710409 1108 4 -0.11516373551219772 1108 5 1.1281367520299028 1108 6 1.2823905091690335 1108 7 0.0691661958465204 1108 8 0.7727903424032263 1108 9 -0.5226311780491244 1108 10-11 0.2628977133382904 1108 12-13 0.4301236582490233 1108 14-15 0.810819518234581 1108 16-17 -0.11004054106134475 1108 18-19 1.240356560277995 1108 20-21 1.2175951636581033 1108 22-23 -0.39906309323075106 1108 24-25 0.21417477534998142 1108 26-27 0.12371429673707013 1108 28-29 0.4259684645437929 1108 30-31 1.3076997594542092 1108 32-33 0.8902616757362267 1108 34-35 0.35853730494022784 1108 36-37 0.6473104077779723 1108 38-39 1.4332795141317973 1108 40-41 0.5277465663770755 1108 42-43 1.2138071246498967 1108 44-45 0.6464620897172537 1108 46-47 1.1784985054098485 1108 48-49 0.4700416655268427 1108 50-51 0.7185961299934291 1108 52-53 0.5851730522865566 1108 54-55 1.6332990090877075 1108 56-57 1.0122287338350944 1108 58-59 -0.850715279646522 1108 60-61 1.3699445734319582 1108 62-63 0.7238943299243203 1108 64-65 0.5939843094273769 1108 66-67 0.814104477797116 1108 68-69 1.6095734213913744 1108 70-71 1.7628792786258956 1108 72-73 1.602932539053981 1108 74-75 1.9491446282861666 1108 76 2.038610575305672 1109 1 -0.6369570268092026 1109 2 -1.5673821501880028 1109 3 -0.18274874697504018 1109 4 -0.25317105928393957 1109 5 0.0813714178828171 1109 6 0.021404851555367088 1109 7 -2.5646452170311136 1109 8 -1.0499896392873467 1109 9 0.28313629219805847 1109 10-11 0.5703054032986188 1109 12-13 -0.5871940103503022 1109 14-15 0.07535262778600327 1109 16-17 -0.44151750169606885 1109 18-19 -0.45353266767440203 1109 20-21 0.23941390030747556 1109 22-23 -0.005257783496233515 1109 24-25 -0.4200562286170637 1109 26-27 0.24234414110691915 1109 28-29 -0.28817556963381463 1109 30-31 0.236025972453902 1109 32-33 0.6645598142775739 1109 34-35 -0.3762429819075024 1109 36-37 -0.6098607853865019 1109 38-39 -0.6519737663076199 1109 40-41 -0.28114876471843786 1109 42-43 0.21174731384734002 1109 44-45 -0.3468244528521751 1109 46-47 -1.073446871459236 1109 48-49 -0.8727642545219823 1109 50-51 0.4561991205335616 1109 52-53 0.0711739677580212 1109 54-55 -0.7611547596031727 1109 56-57 -0.5621914675686277 1109 58-59 -0.9927277910899157 1109 60-61 0.131769413224454 1109 62-63 -0.4339863536277093 1109 64-65 -1.166352889229934 1109 66-67 -0.45096265677719316 1109 68-69 0.8749838577661109 1109 70-71 -0.17410576867346705 1109 72-73 -0.7489518063839213 1109 74-75 1.233627386906857 1109 76 -0.23076690409622103 1110 1 0.9389940725430463 1110 2 -0.03987553582799919 1110 3 0.937495756263715 1110 4 1.0315657348041896 1110 5 0.8827581526697905 1110 6 0.22968876725648357 1110 7 1.2760627475706556 1110 8 1.4858191372841318 1110 9 0.7178594014034232 1110 10-11 -0.7456785397722712 1110 12-13 0.5944500002326762 1110 14-15 0.8123747972960871 1110 16-17 0.7037703370047765 1110 18-19 1.1070913629797374 1110 20-21 0.9180928529577841 1110 22-23 0.4669162882125448 1110 24-25 0.8681474024385025 1110 26-27 -0.7862572638743757 1110 28-29 0.679345641934475 1110 30-31 0.3957285543351219 1110 32-33 1.6356847116409554 1110 34-35 1.0868078346238406 1110 36-37 0.8100370341555063 1110 38-39 0.8919090910958971 1110 40-41 1.3088521476070767 1110 42-43 1.9305685892784155 1110 44-45 2.091183028217067 1110 46-47 1.063630037581909 1110 48-49 1.1023292588435893 1110 50-51 0.7310827990300481 1110 52-53 0.15027259014649275 1110 54-55 0.2642535060660194 1110 56-57 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0.9395159280185013 2116 54-55 1.2080387618528334 2116 56-57 0.2650751879665165 2116 58-59 0.8286113306332439 2116 60-61 -1.2751432600098056 2116 62-63 1.3531233474910138 2116 64-65 1.4943584148275093 2116 66-67 1.4083627731777284 2116 68-69 1.3514406953015907 2116 70-71 -1.0949606693246636 2116 72-73 1.0553560914287345 2116 74-75 -0.21920280177238638 2116 76 0.9021434315190717 2117 1 -0.2252514757231232 2117 2 -1.2994819088364338 2117 3 -0.15036741149635802 2117 4 0.01493030837495013 2117 5 -0.7770356617632004 2117 6 0.628001794435491 2117 7 -2.4284827069747976 2117 8 -0.8040121654337646 2117 9 -1.1762361937231027 2117 10-11 0.44902623998406455 2117 12-13 1.055907357308591 2117 14-15 -0.29341245668704374 2117 16-17 -0.5978932056381439 2117 18-19 0.22154778284853194 2117 20-21 0.5624227498650001 2117 22-23 0.1804823613147022 2117 24-25 1.2020274399267805 2117 26-27 1.071990158806038 2117 28-29 0.10190890341526426 2117 30-31 0.9013517970717615 2117 32-33 1.149274214921185 2117 34-35 0.0125341701439865 2117 36-37 -0.9765140436326831 2117 38-39 0.21266822886533987 2117 40-41 -0.29552929569189246 2117 42-43 -0.2313339411808144 2117 44-45 0.1852551931655242 2117 46-47 -0.27095290524844984 2117 48-49 0.4089131388810685 2117 50-51 0.10221681964860352 2117 52-53 -0.14131597592660938 2117 54-55 0.5819667206864523 2117 56-57 -0.02930731632164907 2117 58-59 -0.10385007588476469 2117 60-61 1.1556028806419931 2117 62-63 0.3563394709901573 2117 64-65 -0.9067993896321838 2117 66-67 -0.9895789077828212 2117 68-69 -1.770128772965986 2117 70-71 1.1128009087199402 2117 72-73 0.8231519747906617 2117 74-75 0.5063546596341304 2117 76 0.2533441176254172 2118 1 0.7747485242768768 2118 2 0.6777908184362929 2118 3 0.008723497594552043 2118 4 0.01493030837495013 2118 5 -0.5270356617632004 2118 6 0.014365430799131218 2118 7 1.3215172930252024 2118 8 -1.0085576199792214 2118 9 -0.2444180119049193 2118 10-11 -0.039610123652309426 2118 12-13 1.0672709936722242 2118 14-15 -0.2138670021415905 2118 16-17 0.5498340670891295 2118 18-19 -0.9716340353332882 2118 20-21 -2.27848634104409 2118 22-23 0.4304823613147022 2118 24-25 -0.7979725600732195 2118 26-27 1.1628992497151316 2118 28-29 0.715545267051624 2118 30-31 1.0377154334353946 2118 32-33 1.6720014876484512 2118 34-35 1.4784432610530729 2118 36-37 0.6257586836400435 2118 38-39 1.087668228865347 2118 40-41 0.045379795217201035 2118 42-43 0.22321151336463885 2118 44-45 0.5943461022564307 2118 46-47 -0.48686199615755044 2118 48-49 -0.23881413384619776 2118 50-51 -0.06823772580594323 2118 52-53 -1.1526796122902425 2118 54-55 -0.4407605520408211 2118 56-57 1.0161472291328977 2118 58-59 0.9188771968425087 2118 60-61 -0.5034880284489134 2118 62-63 0.9813394709901502 2118 64-65 0.6500187921859961 2118 66-67 0.05587563767172554 2118 68-69 1.638962136124924 2118 70-71 -0.6712900003709734 2118 72-73 -0.43821166157297853 2118 74-75 1.165445568725037 2118 76 -0.6102922460109461 2119 1 0.24633943336778685 2119 2 1.6096090002544763 2119 3 0.3070189521400053 2119 4 0.1143621265567667 2119 5 1.3167143382367996 2119 6 0.008683612617311098 2119 7 0.11981274757065563 2119 8 1.576669652748052 2119 9 0.897627442640534 2119 10-11 -0.5296669418341224 2119 12-13 0.8428391754904041 2119 14-15 -1.4865942748688639 2119 16-17 0.021424976180036026 2119 18-19 0.5851841464848917 2119 20-21 -1.171952250135 2119 22-23 0.9446869067692489 2119 24-25 1.0983342581086006 2119 26-27 0.6188651588060381 2119 28-29 0.7837270852334441 2119 30-31 -0.5446709302009651 2119 32-33 0.011490124012091485 2119 34-35 1.2696364428712599 2119 36-37 1.5163836836400435 2119 38-39 0.7069864106835269 2119 40-41 0.40759570430810754 2119 42-43 1.1820183315464519 2119 44-45 1.0446301931655242 2119 46-47 1.3767743674788164 2119 48-49 0.1532313206992555 2119 50-51 0.9147168196486035 2119 52-53 -1.2364864304720626 2119 54-55 0.006682629777358784 2119 56-57 0.6851813200419912 2119 58-59 0.35779765138796193 2119 60-61 1.2266256079147198 2119 62-63 -1.1834332562825765 2119 64-65 1.2281437921859961 2119 66-67 -0.37878345323736795 2119 68-69 -0.10109468205689609 2119 70-71 -0.00651727309824679 2119 72-73 -1.8146321161184318 2119 74-75 1.358627386906857 2119 76 2.6851622994436006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 10.0 13 34.0 14 172.0 15 365.0 16 505.0 17 690.0 18 703.0 19 635.0 20 564.0 21 459.0 22 342.0 23 271.0 24 287.0 25 318.0 26 343.0 27 369.0 28 430.0 29 606.0 30 690.0 31 928.0 32 1209.0 33 1653.0 34 2508.0 35 3820.0 36 6790.0 37 7771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.3691383262095 25.051105742427062 9.604782258564084 27.974973672799358 2 19.62921901946292 22.16371027346637 30.512441488051245 27.69462921901946 3 17.381128356738113 23.894432126139442 28.27358955407736 30.450849963045084 4 12.746366100024636 18.48361665434836 34.192535107169256 34.577482138457746 5 12.281350086228136 36.391352549889135 32.07070707070707 19.25659029317566 6 26.068612958856864 36.16346390736634 18.184897758068487 19.583025375708303 7 25.172456270017246 29.76410445922641 20.37139689578714 24.692042374969205 8 21.932742054693275 35.787755604828774 18.646834195614684 23.632668144863267 9 21.473885193397386 20.605444690810547 20.288248337028826 37.632421778763245 10-11 22.29151268785415 26.13790342448879 26.271864991377186 25.29871889627987 12-13 21.92042374969204 24.83062330623306 26.079391475732937 27.169561468341957 14-15 20.751724562700172 26.019339738851933 23.569536831731956 29.659398866715943 16-17 21.430771125893077 28.349039172209906 24.89529440748953 25.32489529440749 18-19 20.349839862034987 27.412847992116284 26.113266814486323 26.124045331362407 20-21 20.03880266075388 27.703867947770387 26.193335796994333 26.0639935944814 22-23 21.832655826558263 27.47905888149791 25.477334318797734 25.210950973146097 24-25 20.779440748952943 28.801736881005173 26.043976348854397 24.374846021187484 26-27 19.749322493224934 29.23441734417344 26.505912786400593 24.510347376201036 28-29 20.8009977827051 27.87478442966248 27.92867701404287 23.395540773589556 30-31 20.670115792067012 27.44210396649421 27.71772604089677 24.170054200542005 32-33 19.36129588568613 29.49772111357477 26.778455284552845 24.362527716186253 34-35 20.55309189455531 28.168883961566888 27.084873121458486 24.193151022419315 36-37 20.497659522049766 28.071877309682186 27.07717418083272 24.353288987435327 38-39 19.898681941364867 27.548349347129836 28.422949002217297 24.130019709288003 40-41 20.291327913279133 26.801552106430155 27.648435575264845 25.258684405025868 42-43 19.36129588568613 26.969389012071936 28.2889874353289 25.380327666913033 44-45 19.270448386302043 27.047918206454792 28.783259423503328 24.898373983739837 46-47 19.53529194382853 26.967849223946782 28.670854890367085 24.8260039418576 48-49 18.674550381867455 27.499076127124905 28.61080315348608 25.21557033752156 50-51 18.589862034983987 27.362034983986206 28.28590785907859 25.76219512195122 52-53 18.09866962305987 27.06793545208179 28.660076373491005 26.17331855136733 54-55 17.980105937423012 27.73620349839862 28.05032027593003 26.233370288248338 56-57 18.345035723084504 27.63611727026361 27.506775067750677 26.512071938901205 58-59 18.52981029810298 27.095651638334566 27.534491254003452 26.840046809559006 60-61 17.873860556787385 26.958610495195863 27.91019955654102 27.257329391475732 62-63 18.2280118255728 27.04483863020448 27.788556294653855 26.93859324956886 64-65 18.770017245627002 27.3589554077359 26.836967233308695 27.034060113328408 66-67 19.01638334565164 27.82859078590786 25.35569105691057 27.799334811529935 68-69 18.774636610002464 27.716186252771617 25.47579453067258 28.033382606553335 70-71 19.753941857600392 27.098731214584877 25.68058635131806 27.466740576496672 72-73 19.817073170731707 27.07409460458241 25.078529194382853 28.030303030303028 74-75 20.30672579453067 27.141845282089182 24.511887164326186 28.039541759053954 76 20.49150036954915 26.02857846760286 24.6119733924612 28.86794777038679 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.0 14 1.5 15 2.0 16 4.5 17 6.0 18 8.5 19 13.0 20 17.5 21 20.0 22 24.0 23 37.0 24 55.0 25 64.0 26 82.5 27 118.0 28 171.0 29 207.0 30 243.0 31 310.0 32 424.0 33 507.0 34 561.0 35 705.0 36 905.0 37 1015.0 38 1127.0 39 1340.5 40 1610.5 41 1893.5 42 2008.0 43 2009.0 44 2139.0 45 2277.0 46 2286.0 47 2255.5 48 2233.5 49 2135.0 50 2028.0 51 1887.5 52 1601.0 53 1335.5 54 1216.0 55 1173.5 56 1004.5 57 760.5 58 643.0 59 554.0 60 411.5 61 303.5 62 249.0 63 208.0 64 141.5 65 108.0 66 78.5 67 57.0 68 47.5 69 35.0 70 26.5 71 21.0 72 12.5 73 7.5 74 9.0 75 7.0 76 8.5 77 8.5 78 7.5 79 8.0 80 7.0 81 4.5 82 3.5 83 4.0 84 4.0 85 3.5 86 2.0 87 1.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5728011825572801 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 32472.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.6528701650653 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11349018969307 62.121212121212125 2 10.966376292436701 16.592756836659277 3 3.622893429943825 8.222468588322247 4 1.5631360416836277 4.730229120473023 5 0.7652853537409428 2.89480167528948 6 0.4152080110722136 1.8847006651884701 7 0.22388667263697792 1.1856368563685638 8 0.13433200358218678 0.8130081300813009 9 0.08141333550435562 0.5543237250554324 >10 0.11397866970609787 1.0008622813500863 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0030795762503079576 0.0 5 0.0 0.0 0.0 0.0030795762503079576 0.0 6 0.0 0.0 0.0 0.0030795762503079576 0.0 7 0.0 0.0 0.0 0.0030795762503079576 0.0 8 0.0 0.0 0.0 0.006159152500615915 0.0 9 0.0 0.0 0.0 0.006159152500615915 0.0 10 0.0 0.0 0.0 0.009238728750923873 0.0 11 0.0 0.0 0.0 0.009238728750923873 0.0 12 0.0 0.0 0.0 0.009238728750923873 0.0 13 0.0 0.0 0.0 0.009238728750923873 0.0 14 0.0 0.0 0.0 0.015397881251539788 0.0 15 0.0 0.0 0.0 0.015397881251539788 0.0 16 0.0 0.0 0.0 0.015397881251539788 0.0 17 0.0 0.0 0.0 0.018477457501847747 0.0 18 0.0 0.0 0.0 0.018477457501847747 0.0 19 0.0 0.0 0.0 0.02463661000246366 0.0 20 0.0 0.0 0.0 0.02463661000246366 0.0 21 0.0 0.0 0.0 0.02771618625277162 0.0 22 0.0 0.0 0.0 0.02771618625277162 0.0 23 0.0 0.0 0.0 0.03387533875338753 0.0 24 0.0 0.0 0.0 0.03387533875338753 0.0 25 0.0 0.0 0.0 0.03695491500369549 0.0 26 0.0 0.0 0.0 0.046193643754619367 0.0 27 0.0 0.0 0.0 0.06159152500615915 0.0 28 0.0 0.0 0.0 0.09546686375954669 0.0 29 0.0 0.0 0.0 0.11394432126139444 0.0 30 0.0 0.0 0.0 0.1293422025129342 0.0 31 0.0 0.0 0.0 0.1816949987681695 0.0 32 0.0 0.0 0.0 0.218649913771865 0.0 33 0.0 0.0 0.0 0.26484355752648436 0.0 34 0.0 0.0 0.0 0.3202759300320276 0.0 35 0.0 0.0 0.0 0.38802660753880264 0.0 36 0.0 0.0 0.0 0.49889135254988914 0.0 37 0.0 0.0 0.0 0.7113821138211383 0.0 38 0.0 0.0 0.0 1.0039418576003942 0.0 39 0.0 0.0 0.0 1.3673318551367333 0.0 40 0.0 0.0 0.0 1.7553584626755359 0.0 41 0.0 0.0 0.0 2.3651145602365116 0.0 42 0.0 0.0 0.0 3.122690317812269 0.0 43 0.0 0.0 0.0 3.852549889135255 0.0 44 0.0 0.0 0.0 4.5423749692042374 0.0 45 0.0 0.0 0.0 5.182926829268292 0.0 46 0.0 0.0 0.0 5.952820891845282 0.0 47 0.0 0.0 0.0 6.639566395663957 0.0 48 0.0 0.0 0.0 7.200049273220005 0.0 49 0.0 0.0 0.0 7.889874353288987 0.0 50 0.0 0.0 0.0 8.511948755851195 0.0 51 0.0 0.0 0.0 9.112466124661246 0.0 52 0.0 0.0 0.0 9.830007390983 0.0 53 0.0 0.0 0.0 10.63069721606307 0.0 54 0.0 0.0 0.0 11.471421532397143 0.0 55 0.0 0.0 0.0 12.111973392461197 0.0 56 0.0 0.0 0.0 12.783321015028331 0.0 57 0.0 0.0 0.0 13.470066518847007 0.0 58 0.0 0.0 0.0 14.030549396403055 0.0 59 0.0 0.0 0.0 14.584873121458488 0.0 60 0.0 0.0 0.0 15.105321507760532 0.0 61 0.0 0.0 0.0 15.551860064055186 0.0 62 0.0 0.0 0.0 16.112342941611235 0.0 63 0.0 0.0 0.0 16.765213106676523 0.0 64 0.0 0.0 0.0 17.40268539049027 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTACT 15 0.0022059963 69.99538 15 CTTACGC 15 0.0022059963 69.99538 12 AGATCTC 20 0.006893418 52.496532 37 GCTGAGC 20 0.006893418 52.496532 8 CCACTGC 20 0.006893418 52.496532 48 TAGATCT 20 0.006893418 52.496532 36 AGTACGT 20 0.006893418 52.496532 5 GACGAGC 90 0.0051712943 19.443161 69 CCGACGA 145 0.004611634 14.481802 67 GCCGACG 150 0.0057753213 13.999076 66 >>END_MODULE