FastQCFastQC Report
Wed 25 May 2016
SRR1780528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32472
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT9672.9779502340477952No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT5841.7984725301798472No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC3261.0039418576003942No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC1430.4403794037940379No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT890.2740822862774082No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG630.19401330376940135No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG470.14474008376447403No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC430.13242177876324218No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC390.12010347376201036No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC370.11394432126139445No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAA150.00220541970.054
CTACGTC150.00220541970.056
CTTAATA150.00220541970.01
CAGGCGA150.00220541970.07
ACAGGCG150.00220541970.06
TTAATAC150.00220541970.02
ACCACTC252.3564599E-456.00000470
GGCGAAC200.00689161852.4999969
AGGCGAA200.00689161852.4999968
AGGTGTG200.00689161852.4999965
ATTACAG200.00689161852.4999963
CGAACAT200.00689161852.49999611
TCATGAT200.00689161852.49999610
GTTAGGA200.00689161852.4999969
ATTGAAT200.00689161852.49999669
TTAGGAT200.00689161852.49999610
ATACTTA200.00689161852.49999616
TTAATGC200.00689161852.49999641
TACTTAC200.00689161852.49999617
ACAATTG200.00689161852.49999666
Produced by FastQC (version 0.11.5)