FastQCFastQC Report
Wed 25 May 2016
SRR1780527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503718
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTT250.002344150635.2224438
ACCTGGT300.00570733229.3695615
TCGCTAC406.955602E-427.54766820
AACGTTA407.077953E-427.4656248
TATCGTC450.001388678624.46976313
GACTATC450.001391902224.46002838
AGATATC450.001392709124.45759440
CGTTATC450.00139513224.450310
ACGTTAT450.001407299124.413899
CCCGGTC450.001407299124.413898
CCCGTGG500.00255255922.03813620
GCGCTAT602.8380376E-422.0359419
GTAGAGC500.00256883922.01402538
ACATGTT703.2268188E-521.98559629
TCTCGGG1158.867573E-921.01717643
TCAATCC1301.5825208E-920.34289621
GCGACAA654.8858166E-420.33277316
GGCAATG654.8858166E-420.33277316
TCGTCAC654.8858166E-420.33277315
CCGTTTA1301.5970727E-920.32872613