FastQCFastQC Report
Wed 25 May 2016
SRR1780527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503718
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT167603.3272585057512334No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT109412.1720486462663633Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC32580.646790466094124No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG29440.5844540000555867No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC18690.3710409395733327No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC14320.2842860489400815No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT11430.226912677331364No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC7030.13956221536653446No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG6150.12209212297356854No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC5630.11176888655954323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT207.855233E-443.99662435
TGACGAC207.855233E-443.99662419
AAACGTT207.855233E-443.99662435
CGACTTA207.855233E-443.99662422
CCGTCTT11300.040.88181744
GCCGTCT12850.035.9505543
CAGCGTA250.002343227635.2252731
GACGACT250.002352379535.197320
CATTTGC250.002352379535.197329
ACGACAG250.002352379535.197334
ATTGGCG353.2166642E-431.4261621
TTACACA5650.029.9829793
CTTTACA5750.029.4820231
CGTAGCG300.005735669329.3398215
GACGACA300.005744025629.33108533
CGAATTT407.0295524E-427.4978918
TTTACAC6150.027.1876072
CTATACA3150.025.8598231
GCAAAGT450.001397707624.4425722
TGCCGTC18950.024.37807842
Produced by FastQC (version 0.11.5)